JPH0510636Y2 - - Google Patents
Info
- Publication number
- JPH0510636Y2 JPH0510636Y2 JP19333585U JP19333585U JPH0510636Y2 JP H0510636 Y2 JPH0510636 Y2 JP H0510636Y2 JP 19333585 U JP19333585 U JP 19333585U JP 19333585 U JP19333585 U JP 19333585U JP H0510636 Y2 JPH0510636 Y2 JP H0510636Y2
- Authority
- JP
- Japan
- Prior art keywords
- culture medium
- roller
- medium
- type culture
- microorganisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000001963 growth medium Substances 0.000 claims description 24
- 230000002093 peripheral effect Effects 0.000 claims description 4
- 238000000034 method Methods 0.000 description 9
- 244000005700 microbiome Species 0.000 description 9
- 239000002609 medium Substances 0.000 description 8
- 229920001817 Agar Polymers 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【考案の詳細な説明】
〔産業上の利用分野〕
本考案はローラー型培地体、特に微生物採取に
好適なローラー型培地体に関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a roller-type culture medium, particularly a roller-type culture medium suitable for collecting microorganisms.
食品や食品の製造装置あるいは衛生管理が必要
とされる対象物の汚染度を検査するために、一般
にスタンプ法と呼ばれる微生物採取法が広く取り
入れられている。
BACKGROUND ART A microbial sampling method generally referred to as the stamp method is widely used to inspect the degree of contamination of foods, food manufacturing equipment, or objects that require sanitary control.
而して、当該スタンプ法としては、従来ポリエ
チレン等からなる一端を閉じた筒状の袋内に寒天
培地を分注し、開口部を閉じたソーセージ状のも
のを、一方から減菌ナイフで切つて培地面を露出
させ、検査対象物にその面をスタンプした後再び
その面を含むように切り離して減菌シヤーレに入
れて培養する方法と、シヤーレ状の容器に盛り上
がらせて作製した培地を、やはり検査対象物にス
タンプした後フタをして培養する方法の２通りが
ある。 Conventionally, the stamping method involves dispensing agar medium into a cylindrical bag made of polyethylene or the like with one end closed, and cutting the sausage-shaped bag with a sterile knife from one end with the opening closed. A method of stamping the surface of the medium on the test object, cutting it again to include that surface, and placing it in a sterilized shear dish for culturing. There are two methods, one is to stamp the object to be tested, then cover it and culture it.
然しながら、従来の培地面は何れも平面状であ
るため、彎曲面を有する検査対象物より微生物を
採取することは極めて困難なことであつた。
However, since all conventional culture medium surfaces are flat, it is extremely difficult to collect microorganisms from a test object having a curved surface.
そこで、本考案者は斯かる従来の欠点を解消す
べく鋭意検討を重ねた結果、本考案ローラー型培
地体を案出したものである。 Therefore, the inventor of the present invention has made extensive studies to overcome these conventional drawbacks, and has devised the roller-type culture medium of the present invention.
すなわち、本考案は円筒芯体外周面に培地層を
有するローラー型培地体である。
That is, the present invention is a roller type culture medium having a culture medium layer on the outer peripheral surface of a cylindrical core.
以下実施例を示す図面と共に本考案を更に説明
する。
The present invention will be further explained below with reference to drawings showing embodiments.
２はローラー型培地体で、円筒芯体は２２外周
面に培地層２Ｐを有するものである。 2 is a roller type culture medium, and the cylindrical core body 22 has a culture medium layer 2P on its outer peripheral surface.
斯かるローラー型培地体２の具体的製法はその
如何を問わないが、例えば容器体３内に、中空軸
芯部２１を有する円筒芯体２２を、容器体３内壁
との間に適宜間隔３１を形成せしめて挿置し、当
該間隔３１に寒天培地液Ｐを分注して凝固せしめ
た後、該容器体３を取り外して作製するのが簡便
で有利である。尚、その際容器体３としては縦二
分割可能な容易を用いれば、その取り外しが容易
である。また円筒芯体２２としてはその周壁面に
適宜数の突起を形設したものが培地の保持上有利
である。 The specific manufacturing method of such a roller-type culture medium 2 does not matter, but for example, a cylindrical core body 22 having a hollow shaft core 21 is placed in a container body 3, and an appropriate distance 31 is set between the inner wall of the container body 3 and the inner wall of the container body 3. It is simple and advantageous to form and insert the agar medium solution P into the space 31 and solidify it, and then remove the container body 3 and produce it. In this case, if a container body 3 that can be vertically divided into two is used, it can be easily removed. Further, it is advantageous for the cylindrical core body 22 to have an appropriate number of protrusions formed on its peripheral wall surface in terms of retaining the culture medium.
また、ローラー型培地体２の培地は、例えばロ
ーラー面の半分を一般細菌数測定用の標準寒天培
地とし、他半分を真菌用寒天培地とする如く、複
数種類の培地とすれば、一個の培地体２であつて
も、一度に複数種の培地に微生物の採取を行うこ
とができる。 In addition, if the roller-type culture medium 2 has multiple types of media, for example, one half of the roller surface is a standard agar medium for measuring the number of bacteria, and the other half is an agar medium for fungi, one medium can be used. Even in the case of body 2, microorganisms can be collected in multiple types of culture media at once.
ローラー型培地体２の使用に当たつては、例え
ば把持杆１の端部に該ローラー型培地体２を回転
可能かつ着脱自在に取り付けて行なうのが良い結
果を与える。 When using the roller type culture medium 2, it is preferable to rotatably and detachably attach the roller type culture medium 2 to the end of the gripping rod 1, for example, to obtain good results.
培地体２の把持杆１への具体的取り付け法は、
回転可能かつ着脱自在であればその如何を問わな
いが、例えば把持杆１の端部に、支杆１１を介し
て支軸１２を一方向のみに、或いは２方向より対
向的に形成し、当該支軸１２を培地体２の中空軸
芯部２１に挿入軸支せしめて取り付ける方法、或
いは把持杆１の端部に、支軸１２を有する〓体１
３を形設し、当該支軸１２を培地体２の中空軸芯
部２１に挿入軸支せしめて取り付ける方法等が挙
げられる。 The specific method for attaching the culture medium 2 to the gripping rod 1 is as follows.
As long as it is rotatable and detachable, it does not matter. A method of attaching the support shaft 12 by inserting it into the hollow shaft core 21 of the culture medium 2, or attaching the support shaft 12 to the end of the gripping rod 1.
3, and the supporting shaft 12 is inserted into the hollow shaft core 21 of the culture medium 2 to be supported thereon.
尚、斯かる把持杆１を取り付けて微生物の採取
を行つた場合には、採取後該ローラー型培地体２
を把持杆１より取り外し、適宜培養容器４に入れ
て培養すればよい。因に、該培養容器４として
は、例えば培養容器側面に、培地体２の軸芯部２
１を受支する切欠部又は突起を形設するのが、培
地面と容器壁の接触が防止せられるので有利であ
る。 In addition, when collecting microorganisms with such a gripping rod 1 attached, the roller-type culture medium 2 is
may be removed from the gripping rod 1, placed in the culture container 4 as appropriate, and cultured. Incidentally, as for the culture container 4, for example, the axis portion 2 of the culture medium 2 is attached to the side surface of the culture container.
It is advantageous to form a notch or a protrusion for supporting the container 1, since this prevents contact between the culture medium surface and the container wall.
以上の如く本考案は構成されるので、検査対象
面にローラー型培地体２を接触しつつ回転せしめ
ることにより、当該培地面に微生物が採取され
る。
Since the present invention is configured as described above, by rotating the roller type culture medium 2 while contacting the surface to be inspected, microorganisms are collected on the surface of the medium.
従つて、本考案によればスタンプ方式ではなく
回転方式により微生物の採取を行い得るものであ
るため、湾曲面よりの微生物採取をも極めて容易
に行うことができるものである。しかも、本考案
によれば部分回転により好みの部位（培地）に微
生物の採取をなし得るので、効率の良い微生物の
検査培養を行うことができる。
Therefore, according to the present invention, since microorganisms can be collected by a rotation method rather than a stamp method, microorganisms can be collected extremely easily from a curved surface. Moreover, according to the present invention, microorganisms can be collected from a desired site (medium) by partial rotation, so that microorganisms can be tested and cultured efficiently.
図面は本考案の実施例を示すもので、第１図は
把持杆を取り付けた状態の全体斜視図、第２図
１，２，３はそれぞれ把持杆の実施例を示す形態
説明図、第３図は採取状態を示す概略説明図、第
４図は培養容器の斜視図、第５図１，２は本考案
培地体製造工程例を示す概略説明図である。
The drawings show an embodiment of the present invention, and FIG. 1 is an overall perspective view with a gripping rod attached, FIG. 2 is a form explanatory diagram showing an embodiment of the gripping rod, and FIG. The figure is a schematic explanatory diagram showing a state of collection, FIG. 4 is a perspective view of a culture container, and FIGS. 5 1 and 2 are schematic explanatory diagrams showing an example of the manufacturing process of the culture medium of the present invention.
Claims (1)
地体。 A roller type culture medium having a culture medium layer on the outer peripheral surface of a cylindrical core.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19333585U JPH0510636Y2 (en) | 1985-12-16 | 1985-12-16 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19333585U JPH0510636Y2 (en) | 1985-12-16 | 1985-12-16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6299999U JPS6299999U (en) | 1987-06-25 |
| JPH0510636Y2 true JPH0510636Y2 (en) | 1993-03-16 |
Family
ID=31149263
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19333585U Expired - Lifetime JPH0510636Y2 (en) | 1985-12-16 | 1985-12-16 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0510636Y2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7767935B2 (en) * | 2022-01-19 | 2025-11-12 | 株式会社豊田中央研究所 | Method for extracting nucleic acid from biological material and apparatus for extracting nucleic acid from biological material |
-
1985
- 1985-12-16 JP JP19333585U patent/JPH0510636Y2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6299999U (en) | 1987-06-25 |
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