JPH0522513B2 - - Google Patents

Info

Publication number: JPH0522513B2
Authority: JP; Japan
Prior art keywords: plasmid; pig; adenylate kinase; pvu; restriction enzymes
Prior art date: 1987-08-24
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

JP20833387A

Other languages

English (en)

Japanese (ja)

Other versions

JPS6451088A (en

Inventor

Kinichiro Miura

Izumi Kumagai

Takeshi Hibino

Satoru Misawa

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

NITSUKO KYOSEKI KK

Original Assignee

NITSUKO KYOSEKI KK

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-08-24

Filing date

1987-08-24

Publication date

1993-03-29

1987-08-24 Application filed by NITSUKO KYOSEKI KK filed Critical NITSUKO KYOSEKI KK

1987-08-24 Priority to JP20833387A priority Critical patent/JPS6451088A/ja

1989-02-27 Publication of JPS6451088A publication Critical patent/JPS6451088A/ja

1993-03-29 Publication of JPH0522513B2 publication Critical patent/JPH0522513B2/ja

Status Granted legal-status Critical Current

Links

239000013612 plasmid Substances 0.000 claims description 43
108020000543 Adenylate kinase Proteins 0.000 claims description 18
108091008146 restriction endonucleases Proteins 0.000 claims description 13
239000013604 expression vector Substances 0.000 claims description 10
108020005091 Replication Origin Proteins 0.000 claims description 7
238000005520 cutting process Methods 0.000 claims description 6
125000003275 alpha amino acid group Chemical group 0.000 claims description 3
102000002281 Adenylate kinase Human genes 0.000 claims 4
239000012634 fragment Substances 0.000 claims 1
239000002773 nucleotide Substances 0.000 claims 1
125000003729 nucleotide group Chemical group 0.000 claims 1
102100032534 Adenosine kinase Human genes 0.000 description 14
238000000034 method Methods 0.000 description 11
239000002299 complementary DNA Substances 0.000 description 8
230000014509 gene expression Effects 0.000 description 8
241000588724 Escherichia coli Species 0.000 description 6
108090000623 proteins and genes Proteins 0.000 description 6
101100083745 Caenorhabditis elegans pmk-2 gene Proteins 0.000 description 5
108020004705 Codon Proteins 0.000 description 5
102000012410 DNA Ligases Human genes 0.000 description 5
108010061982 DNA Ligases Proteins 0.000 description 5
239000000047 product Substances 0.000 description 5
108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
102000004190 Enzymes Human genes 0.000 description 4
108090000790 Enzymes Proteins 0.000 description 4
229930182817 methionine Natural products 0.000 description 4
125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 4
XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 3
ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 3
XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 description 3
ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 3
UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 3
230000001580 bacterial effect Effects 0.000 description 3
238000005119 centrifugation Methods 0.000 description 3
230000000694 effects Effects 0.000 description 3
244000005700 microbiome Species 0.000 description 3
239000006228 supernatant Substances 0.000 description 3
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
238000002965 ELISA Methods 0.000 description 2
102100034343 Integrase Human genes 0.000 description 2
101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
238000006243 chemical reaction Methods 0.000 description 2
238000009396 hybridization Methods 0.000 description 2
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
238000004519 manufacturing process Methods 0.000 description 2
210000003205 muscle Anatomy 0.000 description 2
238000002703 mutagenesis Methods 0.000 description 2
231100000350 mutagenesis Toxicity 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
102000004169 proteins and genes Human genes 0.000 description 2
239000000523 sample Substances 0.000 description 2
239000013598 vector Substances 0.000 description 2
108010052418 (N-(2-((4-((2-((4-(9-acridinylamino)phenyl)amino)-2-oxoethyl)amino)-4-oxobutyl)amino)-1-(1H-imidazol-4-ylmethyl)-1-oxoethyl)-6-(((-2-aminoethyl)amino)methyl)-2-pyridinecarboxamidato) iron(1+) Proteins 0.000 description 1
QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
108010077805 Bacterial Proteins Proteins 0.000 description 1
108020004414 DNA Proteins 0.000 description 1
102000053602 DNA Human genes 0.000 description 1
108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 description 1
101710203526 Integrase Proteins 0.000 description 1
102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
SVSKFMJQWMZCRD-MCDZGGTQSA-L MgADP Chemical compound [Mg+2].C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]1O SVSKFMJQWMZCRD-MCDZGGTQSA-L 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
108091000080 Phosphotransferase Proteins 0.000 description 1
102000013009 Pyruvate Kinase Human genes 0.000 description 1
108020005115 Pyruvate Kinase Proteins 0.000 description 1
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
238000012258 culturing Methods 0.000 description 1
238000000432 density-gradient centrifugation Methods 0.000 description 1
238000001952 enzyme assay Methods 0.000 description 1
ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
239000000203 mixture Substances 0.000 description 1
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
OJMIONKXNSYLSR-UHFFFAOYSA-N phosphorous acid Chemical compound OP(O)O OJMIONKXNSYLSR-UHFFFAOYSA-N 0.000 description 1
102000020233 phosphotransferase Human genes 0.000 description 1
230000001172 regenerating effect Effects 0.000 description 1
101150039622 so gene Proteins 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
230000003381 solubilizing effect Effects 0.000 description 1
238000010186 staining Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

Genetics & Genomics (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Molecular Biology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Enzymes And Modification Thereof (AREA)

JP20833387A 1987-08-24 1987-08-24 Manifestation vector Granted JPS6451088A (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP20833387A JPS6451088A (en)	1987-08-24	1987-08-24	Manifestation vector

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP20833387A JPS6451088A (en)	1987-08-24	1987-08-24	Manifestation vector

Publications (2)

Publication Number	Publication Date
JPS6451088A JPS6451088A (en)	1989-02-27
JPH0522513B2 true JPH0522513B2 (de)	1993-03-29

Family

ID=16554531

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP20833387A Granted JPS6451088A (en)	1987-08-24	1987-08-24	Manifestation vector

Country Status (1)

Country	Link
JP (1)	JPS6451088A (de)

1987
- 1987-08-24 JP JP20833387A patent/JPS6451088A/ja active Granted

Also Published As

Publication number	Publication date
JPS6451088A (en)	1989-02-27

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