JPH05273218A - Dispensation - Google Patents
DispensationInfo
- Publication number
- JPH05273218A JPH05273218A JP6736392A JP6736392A JPH05273218A JP H05273218 A JPH05273218 A JP H05273218A JP 6736392 A JP6736392 A JP 6736392A JP 6736392 A JP6736392 A JP 6736392A JP H05273218 A JPH05273218 A JP H05273218A
- Authority
- JP
- Japan
- Prior art keywords
- nozzle
- sample
- container
- discharged
- tip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 3
- 238000011109 contamination Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000001174 ascending effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、自動分注装置において
行われる液状試料の分注方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid sample dispensing method performed in an automatic dispensing apparatus.
【０００２】[0002]
【従来の技術】液状試料をノズルによって吸引し他の容
器へ分注を行う自動分注装置が知られており、例えば人
体から採取された血液などの分注を行うものとして用い
られている。2. Description of the Related Art An automatic dispensing device for sucking a liquid sample by a nozzle and dispensing it into another container is known, and is used, for example, for dispensing blood collected from a human body.
【０００３】従来の自動分注装置で行われる分注方法に
ついて説明する。まず、ＸＹＺロボットによって支持さ
れたノズルが試料を入れた試験管上部まで搬送され、そ
の後、ノズル先端が試料内に侵入するまでノズルを降下
させる。その状態でポンプを駆動しノズル内に所定量の
試料を吸引する。その吸引後、ノズルを上昇させ、分注
を行うべき容器までノズルを移動させる。容器上方にノ
ズルが位置した状態で、ノズルを降下させて所定の降下
位置でノズルを停止させ、ノズル内に含まれる試料の一
部または全部を容器へ吐出する。吐出後、ノズルを上昇
させ、他の容器又は初期位置へノズルを移動させる。A dispensing method performed by a conventional automatic dispensing apparatus will be described. First, the nozzle supported by the XYZ robot is conveyed to the upper part of the test tube containing the sample, and then the nozzle is lowered until the tip of the nozzle enters the sample. In that state, the pump is driven to suck a predetermined amount of sample into the nozzle. After the suction, the nozzle is moved up and moved to the container where dispensing is to be performed. With the nozzle positioned above the container, the nozzle is lowered to stop the nozzle at a predetermined lowering position, and a part or all of the sample contained in the nozzle is discharged into the container. After the ejection, the nozzle is moved up and moved to another container or the initial position.
【０００４】ところで、ノズル先端が容器内試料に侵入
しない位置で吐出した場合、容器への試料吐出後ノズル
先端に、試料が球状になって残留することがある。つま
り、ノズル内壁に付着していた試料が降下してノズル先
端外側に試料球が生じる。この最後の一滴は、液体試料
の表面張力によるものであり、その最後の一滴がノズル
先端に付いた状態でノズルの移動を行えば、移動中にい
わゆるぼた落ちにより液状試料が飛散し、汚染を生じさ
せてしまう。By the way, when the sample is discharged at the position where the tip of the nozzle does not enter the sample in the container, the sample may remain in a spherical shape at the tip of the nozzle after the sample is discharged into the container. That is, the sample adhering to the inner wall of the nozzle descends and a sample sphere is formed outside the tip of the nozzle. This last drop is due to the surface tension of the liquid sample.If you move the nozzle while the last drop is attached to the tip of the nozzle, the liquid sample will scatter due to so-called dripping during movement, and contamination will occur. Will be caused.
【０００５】そこで従来においては、吐出位置を下げ、
容器内試料にノズル先端が侵入するようにして試料の吐
出を行っていた。このようにノズル先端を容器内試料に
侵入させて、ゆっくりと上昇させることにより、上述し
た最後の一滴が容器内の試料側へ吸い取られ、ノズル先
端に最後の一滴が残ることが防止される。Therefore, conventionally, the discharge position is lowered,
The sample was discharged with the tip of the nozzle penetrating the sample in the container. In this way, by making the tip of the nozzle enter the sample in the container and slowly ascending, the last drop described above is sucked to the sample side in the container, and the last drop is prevented from remaining at the tip of the nozzle.
【０００６】[0006]
【発明が解決しようとする課題】しかしながら、所定量
ずつ複数の容器へ分注を行う場合には、容器相互間で汚
染が生じるおそれがあり、このためノズル先端を容器内
試料に触れさせずに分注を行う要請がある。つまり、試
料が吐出される容器内に予め試薬などを入れてある所へ
試料の吐出を行う場合があり、容器相互間で試料の混合
が生じることを防止しなければならない。However, when a predetermined amount is dispensed into a plurality of containers, there is a possibility that contamination may occur between the containers, so that the tip of the nozzle should not touch the sample in the container. There is a request to dispense. That is, the sample may be discharged to a place where a reagent or the like is previously put in the container in which the sample is discharged, and it is necessary to prevent mixing of the sample between the containers.
【０００７】ところが、上述したように、ノズル先端が
容器内試料に触れないようにある程度の高さにノズルを
位置して吐出を行えば、ノズル内に残存した試料により
ノズル先端に試料球が残ることになり、ノズル移動中の
試料飛散を防止することができない。However, as described above, if the nozzle is positioned at a certain height so that the tip of the nozzle does not touch the sample in the container and discharge is performed, the sample sphere remains at the tip of the nozzle due to the sample remaining in the nozzle. Therefore, it is impossible to prevent the sample from scattering during the movement of the nozzle.
【０００８】本発明は、上記従来の課題に鑑みなされた
ものであり、その目的は、試料吐出後にノズル先端に残
る試料球を飛散させることなくノズルを移動させること
ができる分注方法を提供することにある。The present invention has been made in view of the above conventional problems, and an object thereof is to provide a dispensing method capable of moving a nozzle without scattering sample spheres remaining at the nozzle tip after sample ejection. Especially.
【０００９】[0009]
【課題を解決するための手段】上記目的を達成するため
に、本発明は、ノズルによって吸引された試料を容器に
吐出する工程の後に、少量の空気を吸引してノズル内先
端にエア層を形成するエア層形成工程が設けられ、前記
エア層をノズル内先端に形成した後、前記ノズルの移動
を行うことを特徴とする。In order to achieve the above object, according to the present invention, after a step of discharging a sample sucked by a nozzle into a container, a small amount of air is sucked to form an air layer at the tip of the nozzle. A step of forming an air layer is provided, and the nozzle is moved after forming the air layer at the tip of the nozzle.
【００１０】[0010]
【作用】上記構成によれば、試料吐出後に、少量の空気
が吸引されノズル内先端にエア層が形成されるので、ノ
ズル先端に一度生じた試料球はノズル内に吸引され、か
つその試料とノズル先端開口との間にはエア層が存在す
るため、ノズルからのぼた落ちを防ぎ、汚染を防止する
ことができる。したがって、ノズル先端を容器内試料に
触れさせずに吐出を行っても試料球による問題を回避で
きる。According to the above structure, after the sample is discharged, a small amount of air is sucked and an air layer is formed at the tip of the nozzle, so that the sample sphere once generated at the tip of the nozzle is sucked into the nozzle and Since the air layer is present between the nozzle tip opening, the nozzle can be prevented from falling down and contamination can be prevented. Therefore, even if discharging is performed without the tip of the nozzle touching the sample in the container, the problem caused by the sample sphere can be avoided.
【００１１】[0011]
【実施例】以下、本発明の好適な実施例を図面に基づい
て説明する。図１には、本発明に係る分注方法の好適な
実施例が示されている。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT A preferred embodiment of the present invention will be described below with reference to the drawings. FIG. 1 shows a preferred embodiment of the dispensing method according to the present invention.
【００１２】この分注方法は、試料の分注を行う自動分
注装置において採用されるものであり、図１には各工程
が模式的に示されている。This dispensing method is adopted in an automatic dispensing apparatus for dispensing a sample, and each step is schematically shown in FIG.
【００１３】ノズル１０によって試料１２が所定量吸引
され、その後、ノズル１０は吐出を行うべき容器１４の
上方に移動され、その後ノズル１０が降下する。なお、
その降下位置として所定のダウン位置が定められ、その
位置は吐出後も容器内試料１６がノズル先端に触れない
高さである。A predetermined amount of the sample 12 is sucked by the nozzle 10, after which the nozzle 10 is moved above the container 14 to be discharged, and then the nozzle 10 descends. In addition,
A predetermined down position is defined as the descending position, and the position is a height at which the in-container sample 16 does not touch the tip of the nozzle even after the ejection.
【００１４】（Ａ）に示すノズル降下工程が終了した
後、図示されていないポンプが駆動され、ノズル１０内
の試料１２が容器１４へ吐出される。After the nozzle lowering step shown in (A) is completed, a pump (not shown) is driven and the sample 12 in the nozzle 10 is discharged into the container 14.
【００１５】すると、（Ｂ）に示すように、ノズル１０
の先端にノズル１０内壁に残存した試料によって試料球
１２ａが付着することになる。もちろん、最後の一滴が
重力によって落下する場合もある。Then, as shown in FIG.
The sample sphere 12a is attached to the tip of the sample sphere by the sample remaining on the inner wall of the nozzle 10. Of course, the last drop may fall due to gravity.
【００１６】その後、（Ｃ）に示すようにノズル１０が
上昇され、あるいはそのダウン位置の状態で、ポンプが
駆動され所定量の空気の吸引が行われる。ここで、その
所定量とは例えば４０μｌである。Thereafter, as shown in (C), the nozzle 10 is raised or in its down position, the pump is driven to suck a predetermined amount of air. Here, the predetermined amount is, for example, 40 μl.
【００１７】（Ｄ）にはエア層２０が示されている。図
示されるように、吸引された試料１２ａの下方にエア層
２０が形成されキャップされているため、いわゆるぼた
落ちが生じることはない。The air layer 20 is shown in FIG. As shown in the figure, since the air layer 20 is formed and capped below the sucked sample 12a, so-called dripping does not occur.
【００１８】そして、エア層２０が形成された後、
（Ｅ）に示すようにノズル１０が初期位置へ戻される。After the air layer 20 is formed,
The nozzle 10 is returned to the initial position as shown in (E).
【００１９】なお、本実施例では、ノズル１０内の試料
１２を、ノズル１０内壁に存在するものを除いて全て吐
出したが、所定量ずつ複数の容器へ分注を行う場合にも
エア層２０の形成は有効である。すなわち、ノズル１０
内の試料１２のうちの一部を吐出した後であっても、試
料球１２ａが付着する場合が多く、エア層２０の形成に
よればそのような試料球をノズル１０内に吸引してノズ
ル１０の移動中に生ずる試料の飛散を有効に防止でき
る。In this embodiment, all the samples 12 in the nozzle 10 except those existing on the inner wall of the nozzle 10 are discharged, but the air layer 20 is also dispensed when a predetermined amount is dispensed into a plurality of containers. Is effective. That is, the nozzle 10
Even after a part of the sample 12 in the inside is ejected, the sample sphere 12a often adheres, and according to the formation of the air layer 20, such a sample sphere is sucked into the nozzle 10 and the nozzle It is possible to effectively prevent the sample from scattering during the movement of 10.
【００２０】[0020]
【発明の効果】以上説明したように、本発明によれば、
試料吐出後にノズル先端内部にエア層を形成したので、
ノズル先端に付着する試料球の飛散による汚染を防止し
て、信頼性の高い分注を行うことができる。As described above, according to the present invention,
Since an air layer was formed inside the nozzle tip after sample ejection,
It is possible to prevent contamination due to scattering of the sample spheres attached to the tip of the nozzle, and perform highly reliable dispensing.
【図１】本発明に係る分注方法の具体的な工程を示す説
明図である。FIG. 1 is an explanatory diagram showing specific steps of a dispensing method according to the present invention.
１０ ノズル １２ 試料 １４ 容器 １６ 容器内試料 ２０ エア層 10 Nozzle 12 Sample 14 Container 16 Sample in Container 20 Air Layer
Claims (1)
吐出する工程の後に、少量の空気を吸引してノズル内先
端にエア層を形成するエア層形成工程が設けられ、前記
エア層をノズル内先端に形成して前記ノズルの移動を行
うことを特徴とする分注方法。1. An air layer forming step of sucking a small amount of air to form an air layer at the inner tip of the nozzle is provided after the step of discharging the sample sucked by the nozzle into the container. A dispensing method characterized in that the nozzle is formed at the tip and the nozzle is moved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4067363A JP2537584B2 (en) | 1992-03-25 | 1992-03-25 | Dispensing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4067363A JP2537584B2 (en) | 1992-03-25 | 1992-03-25 | Dispensing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05273218A true JPH05273218A (en) | 1993-10-22 |
| JP2537584B2 JP2537584B2 (en) | 1996-09-25 |
Family
ID=13342859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4067363A Expired - Lifetime JP2537584B2 (en) | 1992-03-25 | 1992-03-25 | Dispensing method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2537584B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011107089A (en) * | 2009-11-20 | 2011-06-02 | Hitachi High-Technologies Corp | Liquid mixing method and dispensing apparatus |
| JP2011149721A (en) * | 2010-01-19 | 2011-08-04 | Hitachi Plant Technologies Ltd | Reagent aliquot and dispensation method using reagent dispensation nozzle, and reagent aliquot and dispensation mechanism |
| JP2020118600A (en) * | 2019-01-25 | 2020-08-06 | 株式会社日立製作所 | Luminescence measuring device, luminescence measuring system and luminescence measuring method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5320229A (en) * | 1976-07-29 | 1978-02-24 | Tachikawa Spring Co | Structure for head rest of automotive seat |
| JPS5742935U (en) * | 1980-08-25 | 1982-03-09 | ||
| JPS5836355U (en) * | 1981-08-31 | 1983-03-09 | 株式会社島津製作所 | automatic pipette |
| JPS5933211A (en) * | 1982-08-20 | 1984-02-23 | Sato Seiyaku Kk | Oleaginous ointment |
-
1992
- 1992-03-25 JP JP4067363A patent/JP2537584B2/en not_active Expired - Lifetime
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5320229A (en) * | 1976-07-29 | 1978-02-24 | Tachikawa Spring Co | Structure for head rest of automotive seat |
| JPS5742935U (en) * | 1980-08-25 | 1982-03-09 | ||
| JPS5836355U (en) * | 1981-08-31 | 1983-03-09 | 株式会社島津製作所 | automatic pipette |
| JPS5933211A (en) * | 1982-08-20 | 1984-02-23 | Sato Seiyaku Kk | Oleaginous ointment |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011107089A (en) * | 2009-11-20 | 2011-06-02 | Hitachi High-Technologies Corp | Liquid mixing method and dispensing apparatus |
| JP2011149721A (en) * | 2010-01-19 | 2011-08-04 | Hitachi Plant Technologies Ltd | Reagent aliquot and dispensation method using reagent dispensation nozzle, and reagent aliquot and dispensation mechanism |
| JP2020118600A (en) * | 2019-01-25 | 2020-08-06 | 株式会社日立製作所 | Luminescence measuring device, luminescence measuring system and luminescence measuring method |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2537584B2 (en) | 1996-09-25 |
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