JPH0528520Y2 - - Google Patents

Description

[Detailed explanation of the idea]

[Industrial Application Field] This invention relates to a fecal occult blood test tool, specifically a fecal occult blood test to detect blood contained in stool for early diagnosis of cancer, ulcers, etc. in the digestive organs of humans and animals. It is a tool. [Prior Art] A method for chemically or immunologically detecting hemoglobin from human or animal stool specimens is known as a means for early detection of abnormalities in the digestive organs of humans or animals, such as cancer or ulcers. ing. In general, chemical detection methods are extremely sensitive and may result in false positive results, while immunological detection methods require a considerable amount of time for the reaction between hemoglobin and anti-hemoglobin antibodies to proceed to a detectable level. I need it. As a method to improve the drawbacks of fecal occult blood, it has been carried out to use two types of occult blood reactions, a high-sensitivity method and a low-sensitivity method, in combination to determine the presence or absence of bleeding in a stool sample. Such a fecal occult blood test device is as shown in Japanese Patent Application Laid-open No. 190266/1983, and includes a control area having a positive monitor and a negative monitor at the opening of the rear plate of the sheet, and a flap that can be opened and closed at that area. Slides for specimen testing are known. [Problems to be solved by the invention] However, in such fecal occult blood test tools, the guaiac-treated paper easily deteriorates, the test paper becomes water-repellent, and the penetration of the coloring liquid into the test paper is delayed, prolonging the color reaction time. There were flaws. The inventor of the present invention made extensive studies to improve these shortcomings, and found that when such a fecal occult blood test tool was stored in a plastic bag together with a desiccant, the color reaction time was slightly faster. Reached. [Means for Solving the Problem] That is, the present invention is a flat sheet formed by folding flat areas divided by folding lines provided parallel to each other in the lateral direction on a rectangular sheet made of a hydrophobic resin. A fecal occult blood testing device having the following configuration: a bottom area in which a test strip is placed; a protective area connected to the bottom area via a folding line, the end of which fixes one end of the test paper; An intermediate sheet made of a hydrophobic resin having the above-mentioned through-holes, the ends of which are folded over and placed on the other end of the test paper, and the other end placed above the protected area; This fecal occult blood testing tool consists of a space provided between an intermediate sheet and a protective area so as not to touch the paper, and a covered area that covers the intermediate sheet. In addition, the present invention provides the above-mentioned fecal occult blood test tool.
This is a fecal occult blood testing tool in which each reagent paper at a position corresponding to three or more through holes is impregnated with a different reagent. Furthermore, the present invention provides a fecal occult blood testing tool, in which an adhesive tape is attached to the outer or inner surface of the masking area. [Function] The fecal occult blood testing device of the present invention thinly coats the test paper, which is the sample, through the holes in the intermediate sheet, onto the test paper that has been pre-impregnated with a reagent and dried.
The amount of specimen attached can be limited to the size of the through hole. Furthermore, the space provided between the intermediate sheet and the protective area allows sufficient coloring liquid to be poured onto the specimen on the test paper, making it possible to determine the presence or absence of blood in the specimen from the change in color tone. is easy. [Example] Next, an example of the fecal occult blood testing tool of the present invention will be described based on the drawings. FIG. 1 is a sectional view of the fecal occult blood test tool, and FIG. 2 is a perspective view thereof. In the figure, 1 is the covered area of the rectangular sheet, 2 is the bottom area, 3 is the protected area, 4 is the intermediate sheet, 5 is the test paper,
6 is a space, 7 and 8 are through holes, and 9 is an adhesive tape. The rectangular sheet is laterally bent at points A and B in FIG. 1 to form a covered area 1, a bottom area 2 and a protected area 3. Rectangular sheets are made of polyester, polyamide,
Polyolefin, polyvinyl chloride, polycarbonate, polyvinylidene chloride, polyacrylate,
These include sheets made of hydrophobic resins such as polymethacrylate and polystyrene, synthetic paper, and the like. A test paper 5 is placed on the bottom area 2. The test paper 5 is made of filter paper, cotton cloth, etc., and the position of the test paper 5 corresponding to the through hole 7 is impregnated with a reagent such as guaiac or orthotolidine, and then dried, or the test is performed by impregnating and drying the reagent. A piece of paper is made into a shape slightly larger than the size of the through hole 7 and is placed at the position of the test paper corresponding to the through hole 7 for use. The protective area 3 is formed by folding a rectangular sheet along the folding line B, and its end portion is placed on the test paper 5 to fix one end of the test paper 5. The intermediate sheet 4 is a sheet made of hydrophobic resin or synthetic paper having at least one through hole 7, one end of which is placed on the protective area 3, the other end is folded along the folding line C, and the test paper The test strip 5 is placed on the test strip 5 at a position opposite to the protected area 3 of the test strip 5, and the test strip 5 is fixed therein. The intermediate sheet 4 can also be folded along the folding line C using an adhesive or the like. The through hole 7 has a circular, square, etc. shape, and serves to limit the amount of stool sample that adheres to the test strip 5. When testing fecal occult blood using both the high-sensitivity method and the low-sensitivity method, there are two or more through-holes, and the test paper 5 at a position corresponding to each through-hole is impregnated with a reagent, respectively. The space 6 is an area formed by the test paper 5, the protection area 3, and the intermediate sheet 4, and has the effect that the coloring liquid dropped from the through hole 7 undergoes a sufficient coloring reaction. Furthermore, by bending the masked area 1, it can be made into a flattened state. At this time, masked area 1
By providing an adhesive tape on the inner surface of the protection area 3 or the outer surface of the protection area 3, the wall surface of the protection area 3 and the wall surface of the masking area 1 can be fixed. Further, it is preferable that a release sheet is attached to the outer surface of the adhesive tape so that the release sheet can be peeled off during use. Furthermore, when an adhesive tape is provided on the outer surface of the masking area 1, both ends of the adhesive tape 9 can be extended to the outer surface of the bottom area 2 to fix the entire fecal occult blood testing device flat. Examples 1 to 5 In FIGS. 1 and 2, the test paper 5 made of filter paper with a thickness of 0.15 mm was divided into two equal parts in the longitudinal direction. The test paper corresponding to through hole 7 was impregnated with a 0.6% ethanol solution of orthotolidine and then dried. The test paper corresponding to hole 8 is made of guaiac.
It was impregnated in a 1.0% ethanol solution and then dried. Through holes 7 and 8 are circular with a diameter of 30 mm, and the test paper corresponding to through hole 7 contains 0.5 mg of orthotolidine, and the test paper corresponding to through hole 8 contains 0.5 mg of orthotolidine.
Contains 0.8mg of guaiac. In this case, the covering area 1, the bottom area 2 and the protective area 3 are made of polyethylene synthetic paper. After storing the thus manufactured fecal occult blood testing device in an aluminum/polyethylene laminate bag for 3 to 24 months, a hemoglobin aqueous solution was poured into the holes 7 and 8.
The amount of hemoglobin is 0.12~ on the corresponding test paper.
It was dropped with a dropper so that the amount was 1.2 μg. Next, the durability of the test paper was tested by dropping a 3% hydrogen peroxide solution in ethanol and observing the change in color of the through holes 7 and 8. The results are shown in Table 1.

[Table] The orthotolidine reagent gave a greenish blue color, and the guaiac reagent gave a blue color. The presence or absence of fecal occult blood is determined from the measurement results of T method and G method shown in Table 1. If there is no change in the reagent paper with both methods, it is determined that there is no possibility of occult blood, and the T method is ○ and G.
If the result is ×, suspect occult blood and retest, or
Perform other tests. Comparative Examples 1 to 15 In Example 1, a durability test was similarly conducted on a fecal occult blood test tool in which a rectangular sheet was made of paper cardboard instead of polyethylene synthetic paper. When storing fecal occult blood test tools in aluminum/polyethylene laminate bags, durability tests were also conducted in the same way when the bags contained 5 g and 10 g of silica gel. The results are shown in Table 2.

【table】

〔effect〕

In this invention, since the rectangular sheet is made of hydrophobic resin, the moisture in the mount transfers to the test paper and deteriorates the test paper, unlike conventional fecal occult blood test tools whose mount is made of paper. It is water-repellent and does not slow down the penetration of the coloring liquid into the test paper, and does not prolong the coloring reaction time. Therefore, the fecal occult blood testing device of the present invention can be stored for a long period of time and can be used at any time. In addition, in the present invention, since there is sufficient space between the through hole and the test paper, there is no possibility that too much coloring liquid will overflow from the through hole and the reaction will not be carried out sufficiently.

[Brief explanation of drawings]

FIG. 1 is a sectional view of the fecal occult blood test tool, and FIG. 2 is a perspective view thereof. In the figure, 1 is the covered area of the rectangular sheet, 2 is the bottom area, 3 is the protected area, 4 is the intermediate sheet, 5 is the test paper,
6 is a space, 7 and 8 are through holes, and 9 is an adhesive tape.

Claims (1)

[Scope of Claim for Utility Model Registration] (1) Flat-shaped fecal occult blood obtained by folding flat areas divided through folding lines provided parallel to each other in the lateral direction on a rectangular sheet made of a hydrophobic resin. The test tool includes a bottom area where a test paper is placed, a protective area connected to the bottom area via a folding line, and whose end fixes one end of the test paper;
An intermediate sheet made of a hydrophobic resin having at least one through hole, the end portion of which is folded over and placed on the other end of the test paper, and the other end placed over the protected area; a space provided between the intermediate sheet and the protected area so that the holes do not touch the test strip;
and a masked area covering an intermediate sheet. (2) The fecal occult blood testing device according to claim 1, wherein the reagent paper at the position corresponding to the through hole is impregnated with a different reagent. (3) The fecal occult blood testing device according to claim 1 or 2, wherein an adhesive tape is attached to the outer or inner surface of the masking area.