JPH0529265B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to an electrophoresis device, and particularly to a temperature control device thereof.

[Conventional technology]

The conventional device, as described in Japanese Patent Application Laid-Open No. 59-52743, is a small device for use on land, and the temperature, flow rate, etc. of the cooling water are manually adjusted while monitoring the temperature of the liquid in the migration tank. However, no consideration was given to automatic control of the bath liquid temperature.

[Problem that the invention seeks to solve]

The above conventional technology does not take into consideration automatic control of the bath liquid temperature, and when operated unmanned, the bath liquid temperature cannot be properly controlled, so the bath liquid temperature may become too high and kill the biological sample. On the other hand, there was a problem that the cooling water or tank liquid could freeze.

An object of the present invention is to provide a bath liquid temperature control device that automatically controls the temperature of the bath liquid.

[Means for solving problems]

The above purpose is to provide a temperature control device for an electrophoresis device, an electrode power supply device that applies voltage to the electrodes, an ammeter that detects the current flowing through the bath liquid between the electrodes, and an electrode voltage that sends voltage to the electrode power supply device. a setting device, a calorific value calculator that receives the voltage V from the electrode voltage setting device and the current I from the ammeter, and calculates the Joule heat Q of the tank liquid; A tank liquid temperature setting device to set, a cooler to cool the cooling water, a cooling water temperature setting device to set the cooling water temperature Tco, a cooling water temperature detector, and a cooling water temperature setting value from the cooling water temperature setting device. The cooler voltage regulator receives the detected coolant temperature value Tc from the coolant temperature detector and adjusts the voltage of the cooler power supply, and the coolant temperature set value Tco is received from the coolant temperature setter. A cooling water flow rate that receives the tank liquid temperature setting device To from the liquid temperature setting device, the tank liquid temperature detection value T from the tank liquid temperature detector, and the Joule heat Q from the calorific value calculator, and sends the flow rate to the cooling water pump. Consists of a regulator, a cooler power supply device that applies voltage to the cooler, and a cooling water pump that circulates cooling water between the migration tank and the cooler, and adjusts the cooling water flow rate based on the detected tank liquid temperature and cooling water temperature. This is accomplished by providing feedback to the voltage regulators for the cooler and cooler.

[Effect]

The electrophoresis tank is equipped with a tank liquid introduction tube, a sample injection tube at the top, a sample separation section at the bottom, electrodes at both ends, and a member that separates the injected sample into components by electrophoresis.The electrode power supply device is connected to the electrode voltage setting device. Receive the set voltage,
The component that applies the voltage to the electrode, the calorific value calculator, receives the voltage from the electrode voltage setting device and the current from the ammeter that detects the current between the electrodes, calculates the Joule heat of the tank liquid, and cools the calculated value. The voltage regulator for the cooler, which is a component that sends data to the water flow regulator, receives the set temperature Tco from the cooling water temperature setting device, the cooling water temperature (detected value) Tc at the outlet of the cooler from the cooling water temperature detector, and receives the detected value Calculate the voltage that makes Tc equal to the set value Tco,
The cooling water flow rate regulator, which is a component that transmits the calculated value to the cooler power supply, is composed of a subtracter, an adder, a proportional-integral calculator, and a cooling water flow rate calculator. A component that receives the detected temperature T from the detector and the set temperature To from the tank liquid temperature setting device, calculates the cooling water flow rate, and sends the calculated value to the cooling water pump.The tank liquid temperature detector measures the temperature of the tank liquid. A component that detects the detected temperature and sends the detected temperature to the cooling water flow rate regulator.The cooler is a component that cools the cooling water that circulates between the migration tank and the cooler.The cooling water temperature detector detects the temperature of the cooling water at the outlet of the cooler. This is a member that detects the temperature and sends the detected temperature to the voltage regulator for the cooler.

According to the temperature control device of the present invention that combines the above-mentioned members, the electrode voltage, tank liquid temperature, and cooling water temperature are input, the cooling water flow rate is calculated and set, and the detected value of the tank liquid temperature is fed back to the calculated value. Therefore, the cooling water flow rate can be controlled so that the tank liquid temperature is equal to the set value.

〔Example〕

An embodiment of the present invention will be described below with reference to FIG. The main components are migration tank 1, sample injection tube 2, sample separation section 3, tank liquid introduction tube 4, electrode 6, electrode power supply 7, ammeter 8, electrode voltage setting device 9, calorific value calculation cooler 10, cooling water temperature setting device 11, cooler voltage regulator 12, cooler power supply 13, cooler 14, cooling water flow rate regulator 15, tank liquid temperature setting device 1
6, Tank liquid temperature detector 17, Cooling water temperature detector 1
8, a cooling water pump 19, a sample injection pump 20, a sample separation pump 21, a heat absorption surface 22, a heat radiation surface 23, a cooling water flow rate calculator 24, a proportional integral calculator 25, a subtracter 26, and an adder 27. The systems also include a sample injection system 101, a tank liquid introduction system 102, a sample separation system 103, an electrical wiring 104, a cooling water system 10
5. Consists of electrical signal systems 106-125.

The electrophoresis tank 1 is equipped with a tank liquid introduction tube 4 and a sample injection tube 2 at the top, a sample separation section 3 at the bottom, and electrodes 5 and 6 at both ends. The electrodes 5 and 6 are members that separate the sample into components by electrophoresis, and the electrodes 5 and 6 are members that apply an electric field to the sample to electrophoretically separate the sample.The sample separation section 3 is a member that separates the electrophoretically separated components. The electrode power supply device 7 is a member that receives the set voltage V from the electrode voltage setter 9 through the signal system 106 and applies the set voltage V to the electrodes 5 and 6.
The electrode voltage setter 9 sets the voltage to be applied to the electrodes 5 and 6, and sends the set value V to the electrode power supply device 7 via a signal system 106, and to the calorific value calculator 1 via a signal system 107.
The ammeter 8 is a member that detects the current flowing through the bath liquid between the electrodes, and transmits the detected value I to the calorific value calculator 10 via the signal system 108. The calorific value calculator 10 is used to set the voltage for the electrodes. It receives the voltage V from the meter 9 and the current I from the ammeter 8, calculates the Joule heat generation of the tank liquid, and sends the calculated value Q to the cooling water flow rate regulator 15 by the signal system 109 and by the signal system 117. , a member that transmits the signal to the cooler voltage regulator 12, a member that the tank liquid temperature detector 17 detects the temperature of the tank liquid, and a member that transmits the detected temperature T to the cooling water flow rate regulator 15 via the signal system 115; The temperature setting device 16 is a member that sets the tank liquid temperature so that the biological sample does not die, and transmits the set temperature To from the signal system 116 to the cooling water flow rate regulator 15. Set the cooling water temperature above 0℃ and set the temperature Tco
A cooling water flow regulator 15 and a cooler voltage regulator 12
The cooler voltage regulator 12 transmits the set temperature Tco of the cooling water from the cooling water temperature setting device 11 and the cooling water temperature detector 1.
From 8, the detected temperature Tc of the cooling water is calculated from the calorific value calculator 1.
Signal systems 111, 113, 1 respectively calculate the calorific value Q from 0.
17, calculates the voltage so that the set value Tco and the detected value 8c become equal, and transmits the calculated value Vc to the cooler power supply device 13 via the signal system 112.

The cooling water flow rate regulator 15 receives the calorific value Q from the calorific value calculator 10, the set temperature Tco of the cooling water from the cooling water temperature setter 11, the set temperature To of the tank liquid from the tank liquid temperature setter 16, and the set temperature To of the tank liquid from the tank liquid temperature setter 16. The detected temperature T of the tank liquid is received from the temperature detector 17, and the cooling water flow rate G′c is calculated as (=Gc+
ΔG) and transmits the calculated value to the cooling water pump 19 via the signal system 114, the cooler power supply 13 is connected from the cooler voltage regulator 12 to the signal system 11.
2, a member for providing electricity Vc to the cooler 14;
The cooler 14 is a member that cools the cooling water circulating between the migration tank 1 and the cooler 14. The cooling water temperature detector 18 detects the temperature of the cooling water at the outlet of the cooler, and detects the detected temperature Tc.
This is a member that transmits the signal to the cooler voltage regulator 12 via the signal system 113.

FIG. 2 is a detailed diagram of the cooling water flow rate regulator 15. The components are a subtracter 26, an adder 27, a cooling water flow rate calculator 24, and a proportional-integral calculator 25. The cooling water flow rate calculator 24 receives the set temperature Tco of the cooling water from the cooling water temperature setter 11, the calorific value Q from the calorific value calculator 10, the set temperature To of the tank liquid from the tank liquid temperature setter 16, and the set temperature To of the tank liquid from the tank liquid temperature setter 16. The detected temperature T of the tank liquid from the temperature detector 17 is transmitted to signal systems 110, 109, and 11, respectively.
6,115, calculates the cooling water flow rate Gc,
The subtracter 26, which is a member that transmits the calculated value to the adder 27 via the signal system 119, receives the set temperature To from the bath liquid temperature setter 16 and the detected temperature T from the bath liquid temperature detector 17 via the signal systems 120 and 113. , subtract the set value To from the detected value T, and apply the subtracted value to the signal system 12.
1, the proportional integral calculator 25 receives the subtracted value from the subtractor 26, calculates the cooling water flow rate increment ΔGc, and calculates the increment ΔGc.
The adder 27 receives the calculated value Gc of the cooling water from the cooling water flow rate calculator 24 and the increment ΔGc from the proportional integral calculator 25, and adds them together. Addition value Gc+
This is a member that transmits ΔGc to the cooling water pump 19.

FIG. 3 is a detailed diagram of the cooler voltage regulator 12. Components are proportional integral calculator 28, subtracter 2
9, an adder 30, and a voltage calculator 31 for the cooler. The subtractor 29 receives the set temperature Tco of the cooling water from the cooling water temperature setting device 11 and the detected temperature Tc of the cooling water from the cooling water temperature detector 18, respectively, from the signal system 111,
113, subtracts the set value Tco from the detected value Tc, and transmits the subtracted value to the proportional-integral calculator 28 via the signal system 122. The proportional-integral calculator 28 receives the subtracted value from the subtracter 29, and calculates the voltage. The adder 30 receives the increment from the proportional-integral calculator 28 and the voltage Vc from the cooler voltage calculator 31, and sends the increment ΔVc) to the adder 30 via the signal system 123. The added value V′c (=
This is a member that transmits the signal (Vc+ΔVc) to the cooler power supply device 13 via the signal system 125.

According to this embodiment, when the tank liquid temperature, cooling water temperature, and voltage applied to the electrodes are input, the calorific value and cooling water flow rate are calculated and set, and the detected values of the tank liquid temperature and cooling water temperature are fed back to the set values. This has the effect of controlling the bath liquid temperature to be equal to the set value.

〔Effect of the invention〕

According to the present invention, when the bath liquid temperature, electrode voltage, and cooling water temperature are set, the cooling water flow rate corresponding to the calorific value and the applied voltage of the cooler power supply device are calculated, and the detected temperature of the bath liquid is added to the calculated value. Since the cooling water flow rate and the applied voltage of the cooler power supply device can be adjusted by feedback, the tank liquid temperature can be controlled to the set value even if the applied voltage of the electrode is changed.

[Brief explanation of the drawing]

Fig. 1 is an overall configuration diagram of an embodiment of the present invention, Fig. 2
The figure is a detailed diagram of the cooling water flow rate regulator shown in Figure 1.
FIG. 3 is a detailed diagram of the cooler voltage regulator. DESCRIPTION OF SYMBOLS 1...Migration tank, 2...Sample injection tube, 3...Sample separation part, 4...Bath liquid introduction tube, 5...-electrode, 6...+electrode,
7...Electrode power supply device, 8...Ammeter, 9...Electrode voltage setting device, 10...Calorific value calculator, 11...Cooling water temperature setting device, 12...Cooler voltage regulator, 13...Cooler power supply device, 14... Cooler, 15... Cooling water flow rate regulator, 16... Tank liquid temperature setting device, 17... Tank liquid temperature detector, 18... Cooling water temperature detector, 19... Cooling water pump, 20... Sample injection pump, 21 ...sample separation pump, 22...endothermic surface, 23...heat radiation tube, 24...
Cooling water flow rate calculator, 25, 28... Proportional integral calculator, 26, 29... Subtractor, 27, 30... Adder,
31... Voltage calculator for cooler, 101... Sample injection system, 102... Tank liquid introduction system, 103... Sample separation system, 104... Electrical wiring, 105... Cooling water system, 1
06-120...Electrical signal system.

[Claims]

1 A urine collection chamber capable of collecting urine urinated in a toilet bowl is formed, and a cylinder is provided with a slidable piston inside, and a reagent can be sprayed diffusely into the urine collection chamber on the wall surface of the cylinder. 1. A toilet for detecting components in urine, characterized in that a nozzle cap and an oxygen electrode for outputting the amount of oxygen consumed by the reagent in the urine collection chamber to the outside as a current value are attached in a facing manner.