JPH056796Y2 - - Google Patents

Description

[Detailed explanation of the invention] a. Purpose of the invention (industrial application field) The pressure compensator according to this invention is attached to a carbon dioxide incubator, etc., and when the pressure inside the culture container changes from normal pressure, By discharging the gas in the container to the atmosphere or introducing the atmosphere into the container, the pressure in the culture container is maintained at normal pressure.

(Prior Art) When culturing various bacteria in pathological research, etc., carbon dioxide gas incubators are used. In such a carbon dioxide gas incubator, adjustments are made so that the carbon dioxide concentration and oxygen concentration within the culture container are always constant. However, since such adjustment work is performed by newly supplying gas with a lower concentration, the pressure within the culture container increases as the adjustment is made. Furthermore, when the gas in the culture container is consumed as the bacteria being cultured propagate, the pressure in the container decreases.

If such pressure fluctuations are left unaddressed, it may adversely affect the bacterial culture results.
The carbon dioxide incubator is equipped with a compensation device that compensates for pressure fluctuations within the culture container.

FIG. 5 shows a conventional pressure compensation device attached to such a carbon dioxide gas incubator. This pressure compensator consists of two bottles 2 and 3 that store water 1 inside.
Of the two branch pipes 5 and 6 branched from the pipe 4 leading to the culture container, one of the branch pipes 5 is inserted into the water 1 by passing through the lid 7 of one of the bottles 2 in an airtight manner. ,
The other branch pipe 6 passes through the lid 7 of the other bottle 3 in an airtight manner and opens at the top of this bottle 3. 2 bottles each,
3, the lid 7 of one bottle 2 has an exhaust pipe 8 whose lower end opens at the top of the bottle 2, and the lid 7 of the other bottle 3 has an air supply pipe whose lower end is inserted into the water 1. 9 are penetrated airtightly.

When the pressure inside the culture container becomes high, the gas inside this container is discharged to the atmosphere through the pipe 4, the branch pipe 5, and the exhaust pipe 8. Conversely, when the pressure becomes low, the atmosphere is discharged into the air supply pipe 9. , branch pipe 6, and pipe 4 into the culture container. Since gas is always taken in and discharged through water, large amounts of bacteria in the atmosphere will not enter the culture container and adversely affect the culture results.

(Problem to be solved by the invention) However, the pressure compensator that is constructed and operates in the conventional manner prepares two bottles 2 and 3, and attaches the two bottles to the lid 7 attached to each bottle. Because the tube is inserted through the device, the entire device becomes large and requires installation space.

The pressure compensator of the present invention eliminates the above-mentioned disadvantages.

b. Structure of the invention (means for solving the problem) As shown in FIG.
2, and water 1 is injected into both containers 10 and 11 up to a position above the communication path 12. A pipe 4 leading to a container to be pressure compensated, such as a culture container, is connected to a mouth 13 opened on the top surface of one of the two containers 10 and 11, and a pipe 4 that is opened on the top surface of the other container 11 is connected to The opened mouth 14 is opened to the atmosphere, and a sterilization filter 15 is attached if necessary.

(Function) Since the pressure compensator of the present invention is constructed as described above, when the pressure inside the culture container increases, the gas that has entered one of the containers 10 through the tube 4 pushes the top surface of the water 1, and this Water 1 is transferred into the other container 11 as shown in FIG. When the pressure inside the culture container becomes high enough to push the water 1 in one container 10 down to the communication path 12, the gas that has entered this container 10 from the tube 4 will flow through the communication path 12, the other container 11, and the mouth. 14 into the atmosphere.

Conversely, if the pressure inside the culture vessel decreases,
As shown in the figure, the water level in the container 11 on the side open to the atmosphere drops, and air drawn into the container 11 from the mouth 14 flows through the communication passage 12, the container 10, the mouth 11, and the water level in the container 11 drops.
3. The solution is aspirated into the culture vessel through tube 4.

(Application example) In addition, in the example of FIGS. 1 to 3 mentioned above, the container 10,
11 has an independent shape, but the fourth
As shown in the figure, the inside of one container except for the lower end is partitioned by a partition wall 16, and one side of this partition wall 16 functions as the container 10, the other side as the container 11, and the lower side as the communication path 12. good. Furthermore, by making the cross-sectional areas of the containers 10 and 11 different, it is also possible to change the magnitude of the positive pressure when discharging air from the culture container and the negative pressure when sucking air into the culture container.

c. Effects of the invention The pressure compensator of the invention is constructed and operates as described above, but the entire device can be made compact;
The space required for installation is also small.

[Brief explanation of drawings]

Figures 1 to 3 are schematic vertical cross-sectional views showing the pressure compensator of the present invention. Figure 1 shows a steady state, Figure 2 shows a state where the pressure inside the container is high, and Figure 3 shows a state where the pressure inside the container is low. ing. Furthermore, FIG. 4 is a schematic vertical cross-sectional view of a pressure compensator showing an applied example of the present invention, and FIG. 5 a conventional example. 1...water, 2,3...bottle, 4...tube, 5,6...
... Branch pipe, 7 ... Lid, 8 ... Exhaust pipe, 9 ... Air supply pipe, 10, 11 ... Container, 12 ... Communication path, 1
3, 14... Mouth part, 15... Disinfecting filter, 16
...Partition wall.

Claims (1)

[Scope of utility model registration request] The lower portions of a pair of containers are connected to each other through a communication path, and among the openings provided at the upper portions of both containers, one opening is opened to the atmosphere, and the other opening is connected to the container whose pressure is to be compensated. and a pressure compensating device in which liquid is injected into the container up to a position above the communication path.