JPH0598U - Small pieces for culture - Google Patents
Small pieces for cultureInfo
- Publication number
- JPH0598U JPH0598U JP4571791U JP4571791U JPH0598U JP H0598 U JPH0598 U JP H0598U JP 4571791 U JP4571791 U JP 4571791U JP 4571791 U JP4571791 U JP 4571791U JP H0598 U JPH0598 U JP H0598U
- Authority
- JP
- Japan
- Prior art keywords
- culture
- small piece
- shape
- release paper
- piece
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000853 adhesive Substances 0.000 claims abstract description 10
- 230000001070 adhesive effect Effects 0.000 claims abstract description 10
- 239000002985 plastic film Substances 0.000 claims abstract description 6
- 229920006255 plastic film Polymers 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims description 9
- 239000011521 glass Substances 0.000 abstract description 12
- 239000004033 plastic Substances 0.000 abstract description 5
- 229920003023 plastic Polymers 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 2
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 210000000078 claw Anatomy 0.000 description 4
- 239000006059 cover glass Substances 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004820 Pressure-sensitive adhesive Substances 0.000 description 1
- 229920002600 TPX™ Polymers 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 238000003851 corona treatment Methods 0.000 description 1
- 230000005251 gamma ray Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 231100000065 noncytotoxic Toxicity 0.000 description 1
- 230000002020 noncytotoxic effect Effects 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 238000009832 plasma treatment Methods 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
(57)【要約】
【目的】 培養時に、プラスチック製の培養用小片が培
地中に浮き上るのを防止することを目的とする。
【構成】 プラスチック製のフィルムよりなる小片であ
って、片面に細胞及び組織培養に適した処理をほどこ
し、もう一方の片面に粘着剤を塗布して、離型紙に貼り
合せたものである。
【効果】 従来のガラス製スライドグラスの欠点であっ
た破損を防ぎ、かつプラスチック製カバースリップの欠
点であった培養中の浮き上がりがなく、確実にペトリ血
やウェル底面に密着出来、しかもシンプルかつ安価に製
造することが出来る。(57) [Summary] [Purpose] The objective is to prevent plastic culture pieces from floating in the medium during culture. [Structure] A small piece made of a plastic film, one side of which has been subjected to a treatment suitable for cell and tissue culture, the other side of which has been coated with an adhesive and which has been bonded to a release paper. [Effect] Prevents damage, which was a drawback of conventional glass slides, and does not lift up during culture, which was a drawback of plastic coverslips, and can reliably adhere to Petri blood and the bottom of wells, and is simple and inexpensive. Can be manufactured.
Description
【０００１】[0001]
本考案は、組織培養、細胞培養等の分野で使用されるもので、その表面に直接 細胞等を培養し、そのまま染色固定等を行い、標本としうる培養用の小片に関す るものである。 The present invention is used in the fields of tissue culture, cell culture, etc. Incubate cells, etc., perform staining and fixing as they are, and relate to small pieces for culture that can be used as specimens It is something.
【０００２】[0002]
組織培養、細胞培養等の分野においては、培養した組織や細胞の顕微鏡用標本 を製作するには、一般にペトリ皿や複数のウェルを持ったプレート内で組織また は細胞等を培養する際に、顕微鏡用プレパラートを作製する時に使用するガラス 製のカバーグラスを、ペトリ皿またはウェル内に留置し、培地内でそのカバーグ ラス上に組織や細胞等を培養し、培養を終えたら、そのカバーグラスをペトリ皿 やウェルから取出し細胞の固定染色を行なった後、スライドグラス上にそのカバ ーグラスを接着固定して顕微鏡用の標本とする。しかし、この時、使用されるカ バーグラスは非常に破損しやすく、取扱い途中で割れてしまうケースが多いとい う欠点があった。 In fields such as tissue culture and cell culture, microscopic specimens of cultured tissues and cells To make a tissue, tissue or tissue is typically prepared in a Petri dish or a plate with multiple wells. Is a glass used when preparing a microscope preparation when culturing cells etc. Place the glass coverslips in Petri dishes or wells and cover them in culture medium. After culturing the tissues and cells on the lath and finishing the culture, put the cover glass on a Petri dish. After removing the cells from the wells and fixing and staining the cells, cover the cells on a slide glass. -Glue and fix the glass to make a sample for the microscope. However, at this time, the It is said that bar glasses are very fragile and often break during handling. There was a drawback.
【０００３】 そこで本考案者らは、ガラス製カバーグラスの割れやすいという欠点を克服す るため、ガラスの代わりにプラスチック製フィルムを使用し、外周部に２本の切 りこみによって形成した爪部を設けて、取り扱い易くした培養用小片の考案をな し開示した。（実開平３−７００号公報）[0003] Therefore, the present inventors have overcome the drawback that the glass cover glass is easily broken. Therefore, use a plastic film instead of glass, and cut two We have designed a small piece for culture that is easy to handle by providing a nail part formed by re-entry. And disclosed it. (Gazette of Japanese Utility Model Publication No. 3-700)
【０００４】 このようなプラスチックフィルム製の培養用小片は、近年、一般に使用される ようになってきたが、プラスチックの比重がガラスに比べて低いため、培養中、 培地中にフィルムが浮いてしまい、細胞や組織がフィルムの両面に接着し増殖し てしまうという問題があった。[0004] Such culture pieces made of plastic film have been commonly used in recent years. However, since the specific gravity of plastic is lower than that of glass, during culturing, The film floats in the medium and cells and tissues adhere to both sides of the film and grow. There was a problem that it would end up.
【０００５】[0005]
【考案が解決しようとする課題】 本考案は、組織や細胞の培養による顕微鏡用標本製作におけるこのような問題 点を解決しようとしたもので、その目的とするところは、割れにくくペトリ皿や ウェルの培養面に容易に密着でき、かつ構造的にシンプルでコストの安い培養用 の小片を提供することにある。[Problems to be solved by the device] The present invention addresses such problems in microscopic specimen preparation by culturing tissues and cells. It was an attempt to solve the problem, and the purpose is to make it difficult to break and to use a Petri dish or For culture that can easily adhere to the culture surface of the well and is structurally simple and inexpensive To provide small pieces of.
【０００６】[0006]
即ち本考案は、厚さ０．０５〜０．３０mmのプラスチック製フィルムよりなる 円形、半円形、扇形、楕円形もしくは多角形の形状を有する小片であって、該小 片の片面に粘着剤を塗布し、離型紙に貼り合せたことを特徴とする培養用小片で ある。 That is, the present invention comprises a plastic film having a thickness of 0.05 to 0.30 mm. A small piece having a circular shape, a semicircular shape, a fan shape, an elliptical shape, or a polygonal shape. It is a small piece for culture characterized by applying adhesive to one side of one piece and sticking it to release paper is there.
【０００７】 以下、図面により本考案を詳細に説明する。[0007] Hereinafter, the present invention will be described in detail with reference to the drawings.
【０００８】 図１および図２は本考案による培養用小片の形状の例を示す図である。小片（ １）は、プラスチック製フィルムで作られ、その材質としては、顕微鏡で観察す るために透明性を有すること、破損しにくいこと、および耐薬品性を有すること 、組織や細胞を培養する時に毒性を示さないことなどの要件を備えていれば、特 に制限はない。このようなプラスチックとしては、ポリエステル、ポリエチレン 、ポリプロピレン、ＴＰＸ樹脂などがあげられる。また、小片（１）の片面には 、細胞の付着性を良くするために親水化処理を施しても良い。その方法としては 極く一般的には、プラズマ処理、コロナ処理、γ線処理などがある。[0008] 1 and 2 are views showing examples of the shape of a culture piece according to the present invention. Small piece ( 1) is made of plastic film, and its material is observed under a microscope. To be transparent, resistant to breakage, and resistant to chemicals , If there are requirements such as non-toxicity when culturing tissues and cells, There is no limit. Such plastics include polyester and polyethylene , Polypropylene, TPX resin and the like. Also, on one side of the small piece (1) Alternatively, hydrophilic treatment may be performed to improve the adhesion of cells. As a method Most commonly, there are plasma treatment, corona treatment, γ-ray treatment and the like.
【０００９】 もう一方の片面には粘着剤を塗布し、離型紙（４）に貼り合せる。使用時には 離型紙から剥がして使用するが、粘着剤としては透明であることが望ましい。ま た、培養時少なくとも１週間〜１０日間は剥離しない耐水性を有し、培地中への 溶出物による細胞毒性のないものであることが必要で、そのような条件を満たす ものとしては、シリコーン系、ウレタン系、アクリル系などの粘着剤が安全に使 用できる。一方、離型紙としては特に制限はなく、シリコーンコート紙など一般 的に用いられているものが使用できる。[0009] A pressure sensitive adhesive is applied to the other surface of one side, and the release paper (4) is attached. When using It is used by peeling it off from the release paper, but it is desirable that it is transparent as an adhesive. Well In addition, it has water resistance that does not peel off for at least 1 week to 10 days during culturing, and The eluate must be non-cytotoxic and must meet such conditions As a material, adhesives such as silicone-based, urethane-based, acrylic-based can be used safely. Can be used. On the other hand, the release paper is not particularly limited, and is generally used such as silicone-coated paper. What is used conventionally can be used.
【００１０】 粘着剤は小片（１）の全面に塗布してもよいが、培養後のペトリ皿やウェル底 面からの取り出しを容易にするため、小片の端の一部、又は図２に示したように 、小片（１）の周辺部に２本の切り込み（２）によって形成した爪（３）の部分 へは、粘着剤の塗布を避けるのが好ましい。[0010] The adhesive may be applied to the entire surface of the small piece (1), but after the culture, the petri dish and the well bottom To facilitate removal from the surface, part of the end of the strip, or as shown in Figure 2, , The part of the claw (3) formed by two notches (2) on the periphery of the small piece (1) It is preferable to avoid the application of adhesive.
【００１１】 また、粘着剤を塗布した小片は、塗布面を保護し取り扱いを容易にするため、 離型紙に貼り合せて製品化されるが、小片と同形、又は図３（ａ）、（ｂ）に示 したように小片１枚が入る大きさの離型紙片に小片（１）を１枚づつ貼り合せて 、単片の製品としてもよく、あるいは、図３（ｃ）に示したようにシート状の離 型紙（４）に複数枚の小片（１）を貼り合せてカットシート状や長尺の巻重体の 製品にすることもできる。このような製品形態は、培養用小片の使用頻度やユー ザーの使用上の利便などに応じて適宜に選べばよい。[0011] In addition, small pieces coated with an adhesive protect the coated surface and facilitate handling, It is put on a release paper and commercialized, but it has the same shape as a small piece, or as shown in Figures 3 (a) and 3 (b). As shown in the above, attach each piece (1) to a piece of release paper that is large enough to hold one piece. It may be a single piece of product, or it may be separated into sheets as shown in Fig. 3 (c). A plurality of small pieces (1) are attached to the pattern paper (4) to form a cut sheet or a long roll. It can also be a product. This type of product is used for the frequency and usage of culture pieces. It may be appropriately selected according to the convenience of use of the user.
【００１２】 小片（１）の形状は、図１および図２のように円形、楕円形、多角形等適宜に 選べば良く、その大きさと形状は用いられるペトリ皿やプレートのウェルに合わ せて作ることが出来る。また、小片（１）の大きさ、形状を工夫して、半円形や 扇形にすることによって、１つのウェルに複数枚入れて使用することも出来る。 また、図２に示したようにして爪部（３）を設けると、図４に示したように爪部 （３）を折り曲げて、小片（１）を取り扱う際のつまみシロとすることができ、 好ましい。尚、小片（１）の厚さは、０．０５〜０．３０mmの範囲とするのが良 いが、０．０５mmより薄いと形状を保ちにくく、また０．３mmより厚いと顕微鏡 観察の障害となる。[0012] The shape of the small piece (1) may be a circle, an ellipse, a polygon, or the like as shown in FIGS. 1 and 2. You can choose the size and shape that suits the wells of the Petri dish or plate used. You can make it. In addition, by devising the size and shape of the small piece (1), a semicircle or By forming a fan shape, it is possible to use a plurality of sheets in one well. Further, when the claw portion (3) is provided as shown in FIG. 2, the claw portion is provided as shown in FIG. Bending (3) can be used as a knob white when handling the small piece (1), preferable. The thickness of the small piece (1) should be in the range of 0.05 to 0.30 mm. However, if it is thinner than 0.05 mm, it is difficult to maintain its shape, and if it is thicker than 0.3 mm, it is a microscope. Obstruction of observation.
【００１３】 この小片を用いて培養を開始する際は、まず小片（１）を離型紙（４）よりは がし、粘着剤塗布面を下にして、ペトリ皿やウェルの底面に密着させる。その際 、小片（１）の端の一部、又は爪部（３）を折り曲げておき、培養後小片（１） を取り出す際のピンセットによるつまみ部分としておく。[0013] When culturing with this piece, first put the piece (1) over the release paper (4). Peel off and stick it to the bottom of the Petri dish or well with the adhesive coated side down. that time , A part of the end of the small piece (1) or the nail part (3) is bent, and the small piece (1) after culturing Keep it as a knob part with tweezers when taking out.
【００１４】 そして、小片上での細胞培養または組織培養を終えたら、小片（１）をペトリ 皿またはウェルより取り出し、図７に示したように粘着剤によりスライドグラス （７）上に貼り付けて、細胞や組織を固定、染色した後、上よりカバーグラス（ ６）をかけ検体として、保存する。[0014] Then, after the cell culture or tissue culture on the small piece is completed, the small piece (1) is petri Remove from plate or well and slide with adhesive as shown in Figure 7. (7) Stick on top, fix cells and tissues, stain, and then cover glass ( Save 6) as a sample.
【００１５】[0015]
本考案による培養用小片は、従来のガラス製スライドグラスの欠点であった破 損を防ぎ、かつプラスチック製カバースリップの欠点であった培養中の浮き上が りがなく、確実にペトリ皿やウェルの底面に密着出来、しかもシンプルかつ安価 に製造することが出来る。 The small piece for culture according to the present invention has the drawback of the conventional glass slide glass. It prevents the loss and the floating during culture which was a drawback of the plastic coverslip. It is possible to firmly adhere to the bottom of the Petri dish and well without any loss, and it is simple and inexpensive. Can be manufactured.
【図面の簡単な説明】[Brief description of drawings]
【図１】本考案による培養用小片の形状の例を示す図で
ある。FIG. 1 is a view showing an example of the shape of a culture piece according to the present invention.
【図２】本考案による培養用小片の形状の他の例を示す
図である。FIG. 2 is a view showing another example of the shape of the culture piece according to the present invention.
【図３】本考案による培養用小片を離型紙に貼り合せた
状態を示す図である。FIG. 3 is a view showing a state in which a small piece for culture according to the present invention is attached to a release paper.
【図４】（ａ）は爪部を設けた培養用小片の斜視図で、
（ｂ）は爪部をピンセットでつまんで離型紙よりはがす
状態を示す図である。FIG. 4 (a) is a perspective view of a small piece for culture provided with a nail portion,
(B) is a figure which shows the state which pinches a nail | claw part with tweezers and peels from a release paper.
【図５】培養用小片をスライドグラスとカバーグラスの
間に挟んだ検体の構造を示す図である。FIG. 5 is a view showing a structure of a specimen in which a small piece for culture is sandwiched between a slide glass and a cover glass.
Claims (2)
ク製フィルムよりなる円形、半円形、扇形、楕円形もし
くは多角形の形状を有する小片であって、該小片の片面
に粘着剤を塗布し、離型紙に貼り合せたことを特徴とす
る培養用小片。1. A small piece made of a plastic film having a thickness of 0.05 to 0.30 mm, which has a circular shape, a semicircular shape, a fan shape, an elliptical shape, or a polygonal shape, and an adhesive is applied to one surface of the small piece. A small piece for culturing, which is characterized by being attached to a release paper.
を、離型紙上に整列して貼り合せ、カットシート状もし
くは長尺の巻重体としたことを特徴とする培養用小片製
品。2. A small piece product for culture, characterized in that a plurality of small pieces for culture according to claim 1 are aligned and bonded on a release paper to form a cut sheet-shaped or long roll.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4571791U JP2531956Y2 (en) | 1991-06-18 | 1991-06-18 | Small pieces for culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4571791U JP2531956Y2 (en) | 1991-06-18 | 1991-06-18 | Small pieces for culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0598U true JPH0598U (en) | 1993-01-08 |
| JP2531956Y2 JP2531956Y2 (en) | 1997-04-09 |
Family
ID=12727106
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4571791U Expired - Lifetime JP2531956Y2 (en) | 1991-06-18 | 1991-06-18 | Small pieces for culture |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2531956Y2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006059626A1 (en) * | 2004-12-03 | 2006-06-08 | Nipro Corporation | Device for biosample storage |
| JP2013116074A (en) * | 2011-12-02 | 2013-06-13 | Dainippon Printing Co Ltd | Manufacturing method for cell culture vessel |
-
1991
- 1991-06-18 JP JP4571791U patent/JP2531956Y2/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006059626A1 (en) * | 2004-12-03 | 2006-06-08 | Nipro Corporation | Device for biosample storage |
| JP2013116074A (en) * | 2011-12-02 | 2013-06-13 | Dainippon Printing Co Ltd | Manufacturing method for cell culture vessel |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2531956Y2 (en) | 1997-04-09 |
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