JPH0617073Y2 - Electrophoresis device - Google Patents
Electrophoresis deviceInfo
- Publication number
- JPH0617073Y2 JPH0617073Y2 JP11734089U JP11734089U JPH0617073Y2 JP H0617073 Y2 JPH0617073 Y2 JP H0617073Y2 JP 11734089 U JP11734089 U JP 11734089U JP 11734089 U JP11734089 U JP 11734089U JP H0617073 Y2 JPH0617073 Y2 JP H0617073Y2
- Authority
- JP
- Japan
- Prior art keywords
- charged particles
- tube
- migration
- migration tube
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
Description
【考案の詳細な説明】 （イ）産業上の利用分野 この考案は電気泳動装置に関し、特に詳しくは泳動管内
の試料イオンを攪拌する攪拌手段を備えた電気泳動装置
に関するものである。DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention relates to an electrophoretic device, and more particularly to an electrophoretic device equipped with a stirring means for stirring sample ions in a migration tube.
（ロ）従来の技術 一般に電気泳動法は荷電物質、例えば蛋白質，ヘモグロ
ビン，血清，アミノ酸の分離・分析を行う分析法として
知られている。細胞式電気泳動法はこの電気泳動装置の
一種であるが、生理食塩水等の一つの種類の電解液を用
い、この電解液の中に試料を導入し、一定内径0.5〜0.8
mmφの泳動管内で定電流による電気泳動を行い、試料イ
オン（荷電粒子）の泳動速度を光学検出器等を用いて測
定するものである。(B) Conventional Technology Generally, the electrophoresis method is known as an analytical method for separating and analyzing charged substances such as proteins, hemoglobin, serum and amino acids. Cell-based electrophoresis is a kind of this electrophoretic device, but one type of electrolytic solution such as physiological saline is used, and a sample is introduced into this electrolytic solution to obtain a constant inner diameter of 0.5 to 0.8.
The electrophoresis is performed by a constant current in a mmφ migration tube, and the migration speed of sample ions (charged particles) is measured using an optical detector or the like.
しかしこの際、使用する電解液と荷電粒子との比重が不
適合であると、荷電粒子は徐々に泳動管内の底方向に沈
降し始め、泳動速度の測定が終了するまでには、泳動管
内の荷電粒子観測領域（視野）内から外れる荷電粒子が
発生し、その結果測定可能な荷電粒子が減少し、測定精
度が低下したり、場合によっては測定不可能となる場合
もあった。However, at this time, if the specific gravity of the electrolyte used and the charged particles are incompatible, the charged particles gradually begin to settle toward the bottom of the electrophoresis tube, and the charged particles in the electrophoresis tube are charged by the time the measurement of the migration velocity is completed. Charged particles that are out of the particle observation region (field of view) are generated, and as a result, the number of measurable charged particles is reduced, and the measurement accuracy is reduced, or in some cases, measurement is impossible.
このため、泳動管の内径を大きくしたり、又電解液の濃
度をあげたりして、泳動管内の荷電粒子が沈降する時間
を延ばす対策がとられてきたが、両者の対策は共に泳動
管内を透過して泳動管内の荷電粒子の泳動速度を測定す
る検出光の透過率を落とし、結局測定精度が低下するこ
とになる。従って泳動管の内径を小さくし、かつ電解液
の濃度を薄くした状態で荷電粒子の沈降を防止する方法
の出現が望まれていた。For this reason, measures have been taken to increase the inner diameter of the migration tube or increase the concentration of the electrolytic solution to prolong the time for the charged particles in the migration tube to settle. The transmittance of the detection light that passes through and measures the migration speed of the charged particles in the migration tube is reduced, and the measurement accuracy is eventually reduced. Therefore, it has been desired to develop a method of preventing the settling of charged particles in a state where the inner diameter of the migration tube is reduced and the concentration of the electrolytic solution is reduced.
（ハ）目的 この考案はこれらの事情に鑑みなされたもので、泳動管
内を泳動する荷電粒子の沈降を防止し、測定精度を向上
できるようにするものである。(C) Purpose The present invention has been made in view of these circumstances, and is intended to prevent sedimentation of charged particles migrating in an electrophoresis tube and improve measurement accuracy.
（ニ）構成 この考案の構成は、試料イオンを泳動させる泳動管と、
この泳動管の近傍に配設され、泳動管を振動させる超音
波発生器とからなり、該振動によって泳動管内の試料イ
オンを攪拌し浮遊させる電気泳動装置である。(D) Configuration The configuration of the present invention comprises a migration tube for migrating sample ions,
This is an electrophoretic device which is arranged in the vicinity of this migration tube and is composed of an ultrasonic wave generator for vibrating the migration tube, and agitates and suspends the sample ions in the migration tube by the vibration.
すなわち、この考案は、振動手段として超音波発生器を
採用することによって、泳動管内で沈降する荷電粒子を
超音波によって攪拌し、観測点内に存在する荷電粒子の
数を多くして測定精度を向上できる。That is, the present invention employs an ultrasonic wave generator as a vibrating means to stir the charged particles settling in the migration tube with ultrasonic waves and increase the number of charged particles present in the observation point to improve the measurement accuracy. Can be improved.
（ホ）実施例 以下図にしめす実施例に基づいてこの考案を詳述する。
なお、これによってこの考案が限定されるものではな
い。(E) Embodiments The present invention will be described in detail based on the embodiments shown in the drawings.
The invention is not limited to this.
まず、第１，２図において、電気泳動装置１は、冷媒を
満した冷媒槽２と、この冷媒槽２の冷媒内に前後に水平
な泳動管３とこの泳動管３の近傍である冷媒槽２の底面
にこの泳動管を振動させる振動手段である超音波発生器
４とを設け、かつ泳動管路３に前方からマイナス電極部
５，検出部６及びプラス電極部７とを順次備え、さらに
冷媒槽の前方及び後方から冷媒槽２の前後の壁をそれぞ
れ貫通して泳動管３の前端部及び後端部にそれぞれ連結
された試料移送部８及び試料槽部９とから構成される。
なお、１０は家電粒子、１１は検出光、１２，１３は両
電極部５，７と泳動管３とをそれぞれ仕切る隔膜であ
る。First, referring to FIGS. 1 and 2, the electrophoretic device 1 includes a refrigerant tank 2 filled with a refrigerant, an electrophoretic tube 3 horizontal in the refrigerant in the refrigerant tank 2 and a refrigerant tank near the electrophoretic tube 3. An ultrasonic wave generator 4 which is a vibrating means for vibrating the migration tube is provided on the bottom surface of the migration tube 2, and the migration tube path 3 is sequentially provided with a minus electrode portion 5, a detection portion 6 and a plus electrode portion 7 from the front. The sample transfer section 8 and the sample tank section 9 are respectively connected to the front and rear ends of the migration tube 3 through the front and rear walls of the refrigerant tank 2 from the front and rear of the refrigerant tank.
In addition, 10 is a home electric appliance particle, 11 is detection light, and 12 and 13 are diaphragms for partitioning the two electrode parts 5, 7 and the migration tube 3, respectively.
さらに、試料移送部８は泳動管３に連結されたシリンダ
管１４と、このシリンダ管内を往復運動するピストン１
５とからなり、試料槽部９は泳動管３に連結された送液
管１６と、この送液管の開口端を挿入したサンプルカッ
プ１７とからなり、検出部６は検出光１１を検出する光
学検出器１８とこの検出信号を処理する信号処理部１９
とからなる。Further, the sample transfer section 8 includes a cylinder tube 14 connected to the migration tube 3 and a piston 1 that reciprocates in the cylinder tube.
5, the sample tank section 9 is composed of a liquid feed tube 16 connected to the migration tube 3 and a sample cup 17 into which the open end of the liquid feed tube is inserted, and the detection section 6 detects the detection light 11. Optical detector 18 and signal processor 19 for processing the detection signal
Consists of.
以上のように構成される電気泳動装置１において、泳動
管３内の荷電粒子１０の泳動速度は以下のように測定さ
れる。In the electrophoretic device 1 configured as described above, the migration speed of the charged particles 10 in the migration tube 3 is measured as follows.
まず、サンプルカップ１７に電解液、試料を注入し、試
料移送部８のピストン１５を吸込作動させ電解液、試料
を泳動管３内に吸込む。次に両電極部５，７に定電圧を
かけ試料イオン（荷電粒子）１０を泳動管３内を泳動さ
せ、第２図に示すように泳動管３内の荷電粒子１０の観
測領域（点）内を泳動する荷電粒子１０の測定領域
（点）内を泳動する荷電粒子１０に検出光１１を当て、
泳動管３を透過した検出光１１を光学検出部１８、信号
処理部１９でそれぞれ検出処理して荷電粒子１０の泳動
速度を測定する。First, the electrolytic solution and the sample are injected into the sample cup 17, and the piston 15 of the sample transfer unit 8 is suctioned to suck the electrolytic solution and the sample into the migration tube 3. Next, a constant voltage is applied to both electrode parts 5 and 7 to cause the sample ions (charged particles) 10 to migrate inside the migration tube 3, and as shown in FIG. The detection light 11 is applied to the charged particle 10 that migrates in the measurement region (point) of the charged particle 10 that migrates inside,
The detection light 11 transmitted through the migration tube 3 is subjected to detection processing by the optical detection unit 18 and the signal processing unit 19, respectively, and the migration speed of the charged particles 10 is measured.
この泳動速度測定の際、泳動管３内の荷電粒子１０が測
定開始と共に沈降を始め次第に第２図に示すような荷電
粒子１０の観測点から外れて行く。例えばラットの赤血
球では５分程度で沈降する。このため検出光で捕らえら
れる荷電粒子の数が時間の経過と共に減少し、その結果
検出光による測定精度も順次低下する。During the measurement of the migration velocity, the charged particles 10 in the migration tube 3 start to settle at the start of the measurement and gradually deviate from the observation point of the charged particles 10 as shown in FIG. For example, rat red blood cells sediment in about 5 minutes. Therefore, the number of charged particles captured by the detection light decreases with the lapse of time, and as a result, the measurement accuracy of the detection light also decreases.
このような荷電粒子１０の沈降を防止するため超音波発
生器４から発生する超音波により、泳動管３を例えば３
０分間に６０回振動させ泳動管３内に沈降する荷電粒子
１０を攪拌する。なお攪拌の周期は荷電粒子の性質に応
じあらかじめ設定しておく。In order to prevent such sedimentation of the charged particles 10, ultrasonic waves generated from the ultrasonic wave generator 4 cause the migration tube 3 to move to, for example, 3
The charged particles 10 that settle in the migration tube 3 are agitated by vibrating 60 times in 0 minutes. The stirring cycle is set in advance according to the properties of the charged particles.
以上のように荷電粒子を攪拌することによって、常時泳
動管３内に荷電粒子を浮遊させ、荷電粒子１０の観測点
内に存在する荷電粒子１０の数を多くすることができ、
従って荷電粒子１０の泳動速度測定の精度も向上でき
る。By stirring the charged particles as described above, the charged particles can be constantly suspended in the migration tube 3, and the number of the charged particles 10 existing within the observation point of the charged particles 10 can be increased.
Therefore, the accuracy of the migration velocity measurement of the charged particles 10 can be improved.
（ヘ）効果 この考案の効果は泳動管を振動させる超音波発生器を設
け泳動管内の荷電粒子を浮遊させることによって、測定
可能な荷電粒子を多くさせ荷電粒子の泳動速度を容易に
測定でき、かつ測定精度が向上できるようにするもので
ある。(F) Effect The effect of the present invention is to provide an ultrasonic wave generator for vibrating the migration tube to suspend the charged particles in the migration tube, thereby increasing the measurable charged particles and easily measuring the migration velocity of the charged particles. In addition, the measurement accuracy can be improved.
第１図はこの考案に係る電気泳動装置の機能説明断面
図、第２図はこの荷電粒子の検出状態を示す説明図であ
る。 １……電気泳動装置、２……冷媒槽、 ３……泳動管、４……超音波発生器、 ５……マイナス電極部、６……検出部、 ７……プラス電極部、８……試料移送部、 ９……試料槽部。FIG. 1 is a sectional view for explaining the function of the electrophoretic device according to the present invention, and FIG. 2 is an explanatory view showing the detection state of the charged particles. 1 ... Electrophoresis device, 2 ... Refrigerant tank, 3 ... Migration tube, 4 ... Ultrasonic wave generator, 5 ... Minus electrode part, 6 ... Detection part, 7 ... Plus electrode part, 8 ... Sample transfer section, 9 ... Sample tank section.
Claims (1)
動管の近傍に配設され、泳動管を振動させる超音波発生
器とからなり、該振動によって泳動管内の試料イオンを
攪拌し浮遊させる電気泳動装置。1. A migration tube for migrating sample ions, and an ultrasonic generator disposed near the migration tube for vibrating the migration tube. The vibration causes the sample ions in the migration tube to stir and float. Electrophoresis device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11734089U JPH0617073Y2 (en) | 1989-10-05 | 1989-10-05 | Electrophoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11734089U JPH0617073Y2 (en) | 1989-10-05 | 1989-10-05 | Electrophoresis device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0257056U JPH0257056U (en) | 1990-04-25 |
| JPH0617073Y2 true JPH0617073Y2 (en) | 1994-05-02 |
Family
ID=31360715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11734089U Expired - Lifetime JPH0617073Y2 (en) | 1989-10-05 | 1989-10-05 | Electrophoresis device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0617073Y2 (en) |
-
1989
- 1989-10-05 JP JP11734089U patent/JPH0617073Y2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0257056U (en) | 1990-04-25 |
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