JPH06239730A - Whitening cosmetic - Google Patents

Abstract

PURPOSE:To obtain a whitening cosmetic free from skin irritation, excellent in inflammation suppressing effect on skin due to ultraviolet rays and melanogenesis suppressing effect, and further, excellent in hypochromic effect an rapid pigmentation of skin, useful and excellent in feeling of use. CONSTITUTION:A deproteinized material of serum is blended with glabridin. When the deproteinized material of serum alone is blended with other cosmetic raw materials, the feeling of use is not satisfied, though the resultant cosmetic has an antiinflammation effect. When glabridin alone which is hydrophobic ingredients of Glycyrrhiza glabra L. is blended with other cosmetic row materials, it is confirmed to have antimicrobial action, antioxidation action, anticarious action, antiplasmin action and melanogenesis suppressing action, but the feeling of use not satisfied, though the whitening effect is excellent. The blend amount of the deproteinized material of serum is preferably 0.001-3.0wt.% as dried solid amount based on the total amount of cosmetic and the blend amount of glabridin is preferably 0.001-1.0wt.% based on the total amount.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic composition which is excellent in skin safety and has an effect of preventing skin inflammation caused by ultraviolet rays and an effect of rapidly lightening dark skin.

[0002]

2. Description of the Related Art UV rays cause inflammation (erythema) on the skin, and various factors are released to stimulate melanocytes. As a result, the color tone changes and blackens. This blackening is caused by the overproduction of melanin produced in melanocytes and delivered to epidermal cells, and melanin is produced by the oxidation of tyrosine.

Conventionally, in order to prevent skin blackening, stains and freckles and maintain the original white skin, vitamin C salts and fatty acid derivatives which prevent this oxidation, hydroquinone monobenzyl ether, hydrogen peroxide and the like are blended. A whitening cosmetic product has been proposed.

[0004] However, if the vitamin C derivative is added to these whitening cosmetics, the storage stability is insufficient,
In many cases, the effects of suppressing inflammation and whitening by ultraviolet rays are not sufficiently observed. On the other hand, blending hydroquinone monobenzyl ether or the like into a whitening cosmetic has the effect of lightening dark skin, but has the drawback of causing a problem in terms of skin safety. As described above, it is extremely difficult to obtain a whitening cosmetic composition having excellent anti-inflammatory effect and whitening effect, high skin safety, and sufficient storage stability.

When the serum deproteinization product was blended alone, it had an anti-inflammatory effect, but its usability was not satisfactory.

It has been confirmed that when glabridin, which is a hydrophobic component of licorice, is blended alone, it has an antibacterial action, an antioxidant action, an anticariogenic action, an antiplasmin action and a melanin production inhibiting action. However, although the whitening effect was excellent, the feeling of use was not satisfactory.

[0007]

In view of such a situation, the inventors of the present invention have conducted intensive studies to improve the drawbacks of the prior art, and as a result, the invention described below has an excellent anti-inflammatory effect and a whitening effect. Further, they have found that the whitening cosmetic composition has high skin safety and excellent usability, and thus completed the present invention.

[0008] That is, an object of the present invention is to provide a whitening cosmetic composition which is excellent in anti-inflammatory effect and whitening effect, has high skin safety and is excellent in usability.

In order to achieve the above object, the whitening cosmetic composition of the present invention has the following constitution. That is, the present invention is a whitening cosmetic characterized in that it contains indomethacin and glabridin.

The serum deproteinized product used in the whitening cosmetic composition of the present invention can be obtained, for example, by the following method. That is, the fibrin of blood such as a cow as a raw material is removed, acetone is added, and then a mixture of acetone: ether = 1: 1 is added, followed by centrifugation and drying. Distilled water and trypsin are added to the dried product, and fermentation is performed at pH 8 and 370C. After fermentation, cellophane dialysis is carried out and concentrated to obtain an extract. The obtained extract is a yellowish transparent liquid and contains 4 to 5% of solids. These extracts can be purchased as Solcoceryl (manufactured by Solco Basel) or S-71 (manufactured by Botoger).

Glabridin used in the skin cosmetic of the present invention is naturally a kind of licorice, Glycyrrhiza glab.
It is contained in a small amount in a Linne var. (commonly known as Russian, Afghan and Turkish liquorice). As a method for producing glabridin, licorice root or a water extraction residue thereof (for example, a residue obtained by extracting glycyrrhizin) is extracted with an organic solvent. As the extraction solvent, lower aliphatic alcohols such as methanol and ethanol, lower aliphatic ketones such as acetone, dioxane,
One or a mixture of two or more ethers such as ethyl ether, halogenated hydrocarbons such as methylene chloride, esters such as ethyl acetate, and hydrocarbons such as hexane can be used. Licorice to be extracted is about 5-1
It is dipped in 5 times the amount of the above solvent and left standing at room temperature or heated under reflux. The extract obtained by distilling the solvent off from the extract usually contains about 5 to 10% of glabridin. Purification is performed by, for example, a method of treating by normal phase silica gel column chromatography and reverse phase silica gel column chromatography, and then recrystallizing from acetone,
Pure glabridin can be obtained relatively easily.
In addition, purification can be performed by adopting any organic compound purification means such as column chromatography using a synthetic adsorbent.

[0012] The amount of the serum deproteinized protein in the whitening cosmetic composition of the present invention is a dry solid amount, and is preferably 0.001 to 3.0 wt% based on the total amount.

The amount of glabridin incorporated into the whitening cosmetic composition of the present invention is preferably 0.001 based on the total amount.
~ 1.0 wt%.

If the amount of the serum deproteinized product and glabridin is less than 0.001 w%, the effect of the present invention is not sufficient, and the amount of the serum deproteinized protein is 3.0 w% and the amount of glabridin is 1%. Even if it exceeds 0.0 w%, the effect corresponding to the increase cannot be expected to be improved, the feel at the time of use tends to deteriorate, and it becomes difficult to hold individual dosage forms.

The whitening cosmetic composition of the present invention can be formulated into lotions, emulsions, creams, packs and the like according to a conventional method.

In the whitening cosmetic composition of the present invention, dyes, fragrances, preservatives, surfactants, pigments and the like can be appropriately added within the range to achieve the object of the present invention.

[0017]

EXAMPLES The present invention will be described in detail below based on examples and comparative examples.

(1) Tyrosinase activity inhibition test, (2) Skin color lightness recovery test, (3) Whitening practical test, (4) UV erythema inhibition test, (5) Photopatch test, () described in Examples. 6) Each test method of the sensory test is as follows.

(1) Tyrosinase activity inhibition test 0.3 m per 1 ml of McClubein buffer (pH 6.8)
A sample solution of each concentration is added to a tyrosine solution of g / ml concentration, and pre-incubation is performed at 37 ° C for 10 minutes. 1m to this
0.1 ml of tyrosinase (manufactured by Sigma) at a concentration of g / ml
Was added and heated at 37 ° C. for 15 minutes, and then the absorbance (A) was measured at a wavelength of 475 nm using a spectrophotometer. On the other hand, the absorbance (B) of 0.1 ml of buffer solution added in place of tyrosinase, 0.1% buffer solution in place of the sample solution.
The absorbance (C) of the solution added with ml and the absorbance (D) of the sample solution added with 0.2 ml of the buffer solution instead of tyrosinase were measured, and the inhibition rate (%) was calculated according to the following formula.

Inhibition rate (%) = {1- (AB) / (C-
D)} × 100

(2) Skin Color Brightness Recovery Test After the irradiation of the minimum erythema amount of UV rays in the UVA and UVB regions to the skin of the upper arm of 20 test subjects for 3 days continuously,
Reference lightness (V0 value, V of skin of sample application part and base application part)
0'value) was measured. Subsequently, the skin lightness (Vn value, Vn 'value) of the sample-applied part and the base-applied part 1 to 2 weeks after the start of irradiation was applied 3 times a day for 4 consecutive weeks
Was measured, and skin color recovery was evaluated according to the following criteria.

The brightness of the skin (V value of Munsell display system)
Was calculated from X, Y, and Z values obtained by measurement with a high-speed spectrocolorimeter. The evaluation is shown by the average value of the evaluation points of 20 test subjects after 4 weeks.

[0023]

[Table 1] (3) Whitening Practical Test Left forearm flexion side skin of 20 test subjects exposed to summer sunlight for 3 hours (1.5 hours a day for 2 days). Samples from the day of exposure to sunlight,
The base was applied to the right forearm flexion side skin once a day in the morning and continuously for 13 weeks from the day of exposure to sunlight.

The evaluation was shown by the number of test subjects whose effect of the sample application section was confirmed by the base application section.

(4) UV Erythema Suppression Test U was applied to the dorsal skin of 10 dehaired Hartley guinea pigs.
Irradiation with two times the minimum erythema dose of ultraviolet rays in the VB region is performed at two locations each. The sample was applied 24 hours before and immediately after irradiation, the sample application site and the base application site were set, and 24 hours later, the erythema state was evaluated according to the following criteria.

[0027]

[Table 2]

(5) Optical patch test 25 subjects were subjected to closed patch for 24 hours using a patch plate having a diameter of 1.0 cm, which was prepared by applying 0.05 g of the sample to the skin on the forearm flexor side, and then exposed to sunlight in summer. 6 hours (3 a day
For 2 days).

The evaluation was carried out by subject 2 according to the following criteria.
The skin condition of 5 persons was evaluated and judged. Judgment result is irradiation 2
After 4 hours, the number of people was (±) or more.

[0030]

[Table 3] (6) Sensory test Twenty test subjects evaluated the characteristics of the sample after continuously using the sample for 10 days.

The evaluation was shown by the number of respondents who answered that "the skin was moisturized" and "the affinity to the skin was good" for questionnaire items such as wettability and affinity.

Examples 1 to 3 and Comparative Examples 1 to 4 Two-Phase Lotion In the raw material composition of Table 4, the active ingredients were blended as shown in Table 4 to prepare a two-phase lotion, and the above-mentioned various tests Was carried out.

[0033]

[Table 4]

[0034]

[Table 5]

(1) Preparation method Component B shown in Table 4 is uniformly dissolved in component A and component C is uniformly dissolved in component D, and then component A and component D are uniformly mixed and stirred, and then dispersed in a container. To do. At the time of use, the contents should be evenly dispersed by shaking.

(2) Characteristics Table 5 shows the results of various tests. As shown in Table 5, Comparative Example 1 did not show good results in various tests.

The whitening cosmetics of the present invention of Examples 1 to 3 showed clearly good results in all the tests, and no skin irritation occurred in the tests on human skin.

Examples 4 to 7 and Comparative Examples 5 to 7 Skin Cream In the raw material composition of Table 6, active ingredients were blended as shown in Table 7 to prepare a skin cream, and the above-mentioned various tests were carried out.

[0039]

[Table 6]

[0040]

[Table 7]

(1) Preparation method Component B shown in Table 6 is mixed with component A, and component C is uniformly dissolved in component D by heating to bring the temperature to 80 ° C. Then, after injecting and emulsifying the D component in the A component, the mixture is cooled to 300C with stirring.

(2) Characteristics Table 7 shows the results of various tests. As shown in Table 7, Examples 4 to 6 clearly showed good results in all of the tests, good results in the tests on human skin, and no skin irritation occurred.

As described above, the present invention is excellent in the effect of suppressing the inflammation of the skin by ultraviolet rays, the effect of suppressing the production of melanin pigment, the effect of rapidly lightening the skin pigmentation, and the excellent usability without skin irritation. It is clear to provide whitening cosmetics.

Claims (1)

[Claims] 1. A whitening cosmetic composition comprising a serum deproteinization product and glabridin.