JPH0637680Y2 - Biochemistry test equipment - Google Patents
Biochemistry test equipmentInfo
- Publication number
- JPH0637680Y2 JPH0637680Y2 JP5609386U JP5609386U JPH0637680Y2 JP H0637680 Y2 JPH0637680 Y2 JP H0637680Y2 JP 5609386 U JP5609386 U JP 5609386U JP 5609386 U JP5609386 U JP 5609386U JP H0637680 Y2 JPH0637680 Y2 JP H0637680Y2
- Authority
- JP
- Japan
- Prior art keywords
- applicator
- short
- circuit plate
- container
- microwave
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000012360 testing method Methods 0.000 title claims description 6
- 230000005684 electric field Effects 0.000 claims description 12
- 238000010876 biochemical test Methods 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 description 11
- 210000004556 brain Anatomy 0.000 description 10
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012937 correction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000001103 thalamus Anatomy 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
Description
【考案の詳細な説明】 〔産業上の利用分野〕 本考案は、薬理、生化学において、脳内等の細胞内酵素
の研究を目的として、生物供試体にマイクロ波を照射
し、その供試体の酵素を瞬時に失活させる生化学試験装
置に関する。[Detailed Description of the Invention] [Industrial field of application] The present invention irradiates a biological specimen with microwaves for the purpose of studying intracellular enzymes in the brain, etc. in pharmacology and biochemistry. The present invention relates to a biochemical test device that instantly inactivates the enzyme.
マイクロ波により物体を加熱する場合、電子レンジのよ
うなオーブン内にその物体を入れて加熱する方法と、導
波管のようなマイクロ波伝送線路内に入れて加熱する方
法とが考えられる。When heating an object by microwaves, a method of heating the object by placing it in an oven such as a microwave oven and a method of heating it by placing it in a microwave transmission line such as a waveguide can be considered.
特に瞬時に物体を加熱するためには、後者の方法が有効
であるが、導波管の横断面に対してその物体の占める割
合が大きくなると、その導波管の電界分布の影響を受け
て、その物体の被加熱部分における温度差が無視できな
くなるほど大きくなる。The latter method is particularly effective for heating an object in an instant, but when the ratio of the object to the cross section of the waveguide becomes large, it is affected by the electric field distribution of the waveguide. , The temperature difference in the heated portion of the object becomes so large that it cannot be ignored.
第６図は伝送線路を利用した従来のマイクロ波加熱装置
を示すものであり、矢印Ｅ方向に電界が生じるTE10モー
ドとして働かせる導波管を伝送線路として使用し、この
導波管の終端を短絡板11により短絡してアプリケータ１
を形成し、そのアプリケータ１の一方の長辺12における
短絡板11から所定距離だけ離れた位置に挿入口13を形成
し、その挿入口13からラット等の生物供試体２の頭部21
を挿入したものである。FIG. 6 shows a conventional microwave heating device using a transmission line. A waveguide that acts as a TE 10 mode in which an electric field is generated in the direction of arrow E is used as the transmission line, and the end of this waveguide is used. Applicator 1 short-circuited by short-circuit plate 11
Is formed, and an insertion port 13 is formed at a position separated from the short-circuit plate 11 on one long side 12 of the applicator 1 by a predetermined distance, and the head 21 of the biological specimen 2 such as a rat is formed from the insertion port 13.
Is inserted.
即ち、この供試体２は、供試体容器３内に押込部材４に
よって、頭部21を先端として押し込まれ、係止板５によ
ってその押込部材４を供試体容器３の切込み33に対して
固定することにより、その全体の動きが固定されてい
る。41は押込部材４の軸、42は係止板５が係止する押込
部材４の溝である。That is, the specimen 2 is pushed into the specimen container 3 by the pushing member 4 with the head 21 as the tip, and the pushing member 4 is fixed to the notch 33 of the specimen container 3 by the locking plate 5. By that, the whole movement is fixed. 41 is a shaft of the pushing member 4, and 42 is a groove of the pushing member 4 with which the locking plate 5 is locked.
そして、供試体容器３の胴体部を覆う透明の本体31をア
プリケータ１の挿入口13の外側を囲むように取り付けた
チャンバ６内に挿入することにより、供試体２の頭部21
を覆う筒部32がアプリケータ１内に突出するようにして
いる。なお、この筒部32には、マイクロ波透過性の良好
な低損失、低誘電率の材質、例えばプラスチックが使用
されている。Then, the transparent main body 31 that covers the body of the sample container 3 is inserted into the chamber 6 that is attached so as to surround the outside of the insertion port 13 of the applicator 1, and thus the head 21 of the sample 2 is inserted.
The tubular portion 32 that covers the inside of the applicator 1 projects into the applicator 1. The tubular portion 32 is made of a material having a low microwave loss and a low loss and a low dielectric constant, such as plastic.
ところが、このマイクロ波加熱装置では、マイクロ波が
TE10モードでアプリケータ１内を矢印Ａ方向に進む際
に、そのアプリケータ１内の電界Ｅの分布は、第７図
（ａ）に示すように導波管の軸中心ａが最大となり、電
力（電界Ｅの二乗に比例する）はその軸中心ａが著しく
強くなる。However, in this microwave heating device, the microwave
When traveling in the direction of arrow A in the applicator 1 in the TE 10 mode, the distribution of the electric field E in the applicator 1 is maximized at the axial center a of the waveguide as shown in FIG. 7 (a). The electric power (proportional to the square of the electric field E) becomes significantly strong at its axial center a.
従って、供試体２の加熱すべき頭部21の容積・寸法が使
用マイクロ波のλｇ（管内波長）に比較して非常に小さ
い場合には、その頭部21の加熱された内部の温度差は無
視できるほどに小さいが、加熱すべき部分が大きくなる
につれて、電界分布の影響を受け易くなり、加熱された
内部の温度差が無視できないほど大きくなる。Therefore, when the volume / dimension of the head 21 to be heated of the sample 2 is very small compared to λg (wavelength in the tube) of the microwave used, the temperature difference inside the heated portion of the head 21 is Although it is negligibly small, the larger the portion to be heated, the more easily it is affected by the electric field distribution, and the temperature difference inside the heated portion becomes so large that it cannot be ignored.
特に、頭部21の両側面が充分加熱されるまで電波を放射
すると、軸中心ａ上の部分が過剰加熱の状態となり、そ
の軸中心ａ上に存在する重要で微細な脳22の組織（視床
下部等）が破壊され易い。In particular, when radio waves are radiated until both sides of the head 21 are sufficiently heated, the part on the axis center a is overheated, and the important minute tissue of the brain 22 existing on the axis center a (thalamus). Lower part) is easily destroyed.
また、加熱中に供試体２の頭部21が第７図（ｂ）に示す
ように矢印Ｂ方向に少しでも回転すると、その頭部21の
加熱による左右の温度差が例えば20℃以上に大きくな
り、その頭部21の脳22内を研究対象とする場合には、そ
の脳22の片側が過剰加熱、他側が加熱不足という状態と
なる。この結果脳21の部位を均一に加熱して脳内酵素分
布を調査する研究に支障をきたすことがある。When the head 21 of the test piece 2 is rotated in the direction of arrow B even during the heating as shown in FIG. When the inside of the brain 22 of the head 21 is to be studied, one side of the brain 22 is overheated and the other side is underheated. As a result, it may interfere with the research for uniformly heating the region of the brain 21 and investigating the enzyme distribution in the brain.
本考案の目的は、供試体の被加熱部がほぼ均一に加熱さ
れるようにした生化学用試験装置を提供することであ
る。An object of the present invention is to provide a biochemical test device in which a heated portion of a test piece is heated almost uniformly.
このために本考案は、片側に短絡板を備えたアプリケー
タと、生物供試体の頭部を覆う筒部及び胴体部を覆う本
体で形成した容器とを具備し、上記筒部を上記アプリケ
ータ内に突出させてマイクロ波を照射するようにした生
化学用試験装置において、 上記容器の筒部を上記マイクロ波によって発生する電界
の最小値の位置に設定するために、上記短絡板をその位
置が上記アプリケータ内において可変となるように構成
した。To this end, the present invention comprises an applicator having a short-circuit plate on one side, and a container formed by a tube portion that covers the head of the biological specimen and a body that covers the body portion, and the tube portion is the applicator. In the biochemical test device which is configured to project into the interior and irradiate with microwaves, in order to set the tubular portion of the container to the position of the minimum value of the electric field generated by the microwaves, the short-circuit plate is placed at that position. Is configured to be variable in the applicator.
以下、本考案の実施例について説明する。第１図はその
実施例の断面を示し、該図において、アプリケータ７の
短絡板71はピストン形に形成されアプリケータ７内を軸
方向に移動してその位置が可変となるように構成されて
いる。71aはその短絡板71を移動させるためのロッドで
ある。なお、このアプリケータ７はその端面が金属板74
で閉じられている。Embodiments of the present invention will be described below. FIG. 1 shows a cross section of the embodiment, in which the short-circuit plate 71 of the applicator 7 is formed in a piston shape and is configured to move axially in the applicator 7 so that its position is variable. ing. 71a is a rod for moving the short-circuit plate 71. The end surface of the applicator 7 is a metal plate 74.
Closed in.
一方、チャンバ６はアプリケータ７の短片（Ｅ面）72側
に設けられ、その短片72側には、該チャンバ６で囲まれ
る孔73が形成されている。そして、そのチャンバ６から
孔73にかけて、供試体容器８が装填されている。On the other hand, the chamber 6 is provided on the short piece (E surface) 72 side of the applicator 7, and the hole 73 surrounded by the chamber 6 is formed on the short piece 72 side. Then, the sample container 8 is loaded from the chamber 6 to the hole 73.
この供試体容器８は、供試体２の胴部が入る本体81を、
その本体81と同一内外径でその供試体２の頭部21が入る
筒部82とで構成されている。この筒部82には例えばマイ
クロ波透過性の良好な低損失・低誘電率の材質が選択さ
れる。そして、該筒部82が、孔73からアプリケータ７内
に、そのアプリケータ７の中心より若干先方に先端が位
置するように、ガイド61とストッパ86により、規制され
て突出している。85は開口面84を閉じるための電波漏れ
防止用蓋である。The sample container 8 includes a main body 81 in which the body of the sample 2 is inserted.
The main body 81 has the same inner and outer diameters as that of the cylindrical portion 82 into which the head 21 of the sample 2 is inserted. For the cylindrical portion 82, for example, a material having a low microwave loss and a low loss and a low dielectric constant is selected. Then, the tubular portion 82 is regulated and projected by the guide 61 and the stopper 86 so that the tip is located in the applicator 7 through the hole 73 and slightly ahead of the center of the applicator 7. Reference numeral 85 is a radio wave leakage prevention lid for closing the opening surface 84.
供試体２の容器８内への押し込みは、従来と同様に押込
部材９と係止片５とによって行われ、これにより、第１
図に示すように、その供試体２の頭部21が、アプリケー
タ７のほぼ中心に位置するようになっている。The pushing of the sample 2 into the container 8 is performed by the pushing member 9 and the locking piece 5 as in the conventional case, whereby the first
As shown in the figure, the head portion 21 of the test piece 2 is located substantially at the center of the applicator 7.
以上より、短絡板71を移動すれば、その短絡板71と供試
体容器８の筒部82の中心軸との距離ｌを任意に変更する
ことができる。From the above, by moving the short-circuit plate 71, the distance 1 between the short-circuit plate 71 and the central axis of the tubular portion 82 of the sample container 8 can be arbitrarily changed.
そこで、上記距離ｌがマイクロ波の管内波長の1/2とな
るように上記短絡板71の位置を調節してアプリケータ７
内にマイクロ波を矢印Ａ方向から照射すると、第３図の
ように上記筒部82の位置に電界Ｅの最小値の位置が一致
する。Therefore, the position of the short-circuit plate 71 is adjusted so that the distance 1 becomes half the wavelength of the microwave in the tube, and the applicator 7 is moved.
When microwaves are radiated inside from the direction of arrow A, the position of the minimum value of the electric field E coincides with the position of the cylindrical portion 82 as shown in FIG.
この場合、筒部82内に固定された生物供試体２の頭部21
は第４図のように頭部21の側面から電界Ｅを受けて加熱
されが、その頭部21内には第５図で示されるように、温
度関係がT0＜T1＜T2…＜Tnで、等温線が同心円で、か
つ、その温度差が少ない温度分布となる。In this case, the head 21 of the biological specimen 2 fixed in the tubular portion 82
Is heated by receiving the electric field E from the side surface of the head portion 21 as shown in FIG. 4, and the temperature relation in the head portion 21 is T 0 <T 1 <T 2 ... As shown in FIG. <T n , the isotherms are concentric circles, and the temperature distribution has a small temperature difference.
これにより、頭部21内の脳22はその中心部とその両側、
及びその上下面のそれぞれの温度差が少なく、全体とし
て比較的均一な温度分布となり、好適な加熱ができる。
以上は実験的に確認されている。As a result, the brain 22 in the head 21 has its center and both sides thereof,
Also, the temperature difference between the upper and lower surfaces is small, and the temperature distribution is relatively uniform as a whole, so that suitable heating can be performed.
The above has been confirmed experimentally.
また、上記短絡板71と筒部82との距離を管内波長の1/2
の整数倍となるように調整した場合も、脳22に対する加
熱は上述したものと全く同じ結果となる。In addition, the distance between the short-circuit plate 71 and the tubular portion 82 is 1/2 of the guide wavelength.
Even when it is adjusted to be an integral multiple of, the heating of the brain 22 has exactly the same result as described above.
更に、短絡板を可変構造としないで短絡板と筒部82との
距離ｌを管内波長の1/2又は1/2の整数倍となるように固
定したものでも同様の結果を得る。Further, the same result can be obtained by fixing the distance l between the short-circuit plate and the cylindrical portion 82 to be 1/2 or an integral multiple of 1/2 of the in-tube wavelength without using the variable structure of the short-circuit plate.
更にまた、上記容器８の本体部81の開口面84に着脱可能
な電波漏れ防止用の蓋85を設けてあるので、試験装置の
外部に対する電波障害の発生を防止できる。Furthermore, since the detachable lid 85 for preventing electric wave leakage is provided on the opening surface 84 of the main body 81 of the container 8, it is possible to prevent the occurrence of radio wave interference to the outside of the test apparatus.
第２図は、本考案の他の実施例であって、チャンバ６、
ストッパ86′及び供試体容器８′がそれぞれ相互に同心
円に形成され、ストッパ86′が直接挿入口73の縁部に係
止し、該容器８′がチャンバ６内において360度全回転
できるように構成してある。FIG. 2 shows another embodiment of the present invention, which is a chamber 6,
The stopper 86 ′ and the specimen container 8 ′ are formed concentrically with each other, and the stopper 86 ′ is directly engaged with the edge of the insertion port 73 so that the container 8 ′ can be fully rotated 360 degrees in the chamber 6. Configured.
従って、本実施例においては、生物供試体２が収納され
た供試体容器８′をアプリケータ７に装着した場合に供
試体２の頭部21の向きが正しい方向よりずれても容器８
を回転させることにより、簡単にこれを修正することが
できる。Therefore, in this embodiment, when the specimen container 8 ′ containing the biological specimen 2 is attached to the applicator 7, even if the orientation of the head 21 of the specimen 2 deviates from the correct direction, the container 8
You can easily fix this by rotating.
これに対し、前述の実施例（第１図）においてはチャン
バ６と供試体容器８とは偏心し、かつ、ストッパ86は半
周状に形成されているため、供試体容器８が回転してス
トッパ86がチャンバ６に触れた時点でその回転は停止さ
れる。従って、上記の如き頭部21の向き修正をしなけれ
ばならない場合に、その修正ができない恐れがあった。On the other hand, in the above-described embodiment (FIG. 1), the chamber 6 and the specimen container 8 are eccentric, and the stopper 86 is formed in a semicircular shape. When 86 touches chamber 6, its rotation is stopped. Therefore, when the orientation of the head 21 must be corrected as described above, there is a fear that the correction cannot be performed.
以上から本考案によれば、供試体の頭部が挿入される筒
部をマイクロ波によって発生する電界の最小値の位置に
設定したことにより、頭部の脳に対する加熱による温度
分布が均一化され、良好な実験データを取得することが
できる。From the above, according to the present invention, the temperature distribution due to the heating of the brain of the head is made uniform by setting the cylindrical portion into which the head of the sample is inserted to the position of the minimum value of the electric field generated by the microwave. , Can obtain good experimental data.
第１図は本考案の実施例の生化学用試験装置を示し、
（ａ）はその縦断面図、（ｂ）は（ａ）図のX-Xに沿っ
た断面図、第２図は本考案の他の実施例を示し、（ａ）
はその部分縦断面部、（ｂ）は（ａ）図のY-Yに沿った
断面図、第３図はアプリケータ内の電界の状態を示す
図、第４図は供試体容器の筒部に対する電界の状態を示
す図、第５図は供試体の頭部の温度分布を示す図、第６
図は従来の生化学用試験装置の断面図、第７図（ａ）は
アプリケータ内における電界及び電力の分布を示す図、
（ｂ）は第６図のZ-Zに沿った断面図である。 ２……供試体、21……頭部、22……脳、７……アプリケ
ータ、71……短絡板、71a……ロッド、8,8′……供試体
容器、81……本体、82……筒部、84……開口面、85……
蓋、ｌ……距離。FIG. 1 shows a biochemical test apparatus according to an embodiment of the present invention.
(A) is a longitudinal sectional view thereof, (b) is a sectional view taken along line XX of (a), and FIG. 2 shows another embodiment of the present invention, (a)
Is a partial longitudinal section thereof, (b) is a sectional view taken along the line YY of (a), FIG. 3 is a view showing a state of an electric field in the applicator, and FIG. FIG. 5 is a diagram showing the state, FIG. 5 is a diagram showing the temperature distribution of the head of the specimen, and FIG.
FIG. 7 is a cross-sectional view of a conventional biochemical test device, and FIG. 7 (a) is a view showing distribution of electric field and electric power in an applicator,
6B is a sectional view taken along the line ZZ in FIG. 2 ... Specimen, 21 ... Head, 22 ... Brain, 7 ... Applicator, 71 ... Short-circuit plate, 71a ... Rod, 8,8 '... Specimen container, 81 ... Main body, 82 …… Cylinder part, 84 …… Opening surface, 85 ……
Lid, l ... distance.
Claims (4)
物供試体の頭部を覆う筒部及び胴体部を覆う本体で形成
した容器とを具備し、上記筒部を上記アプリケータ内に
突出させて上記短絡板と反対方向からマイクロ波を照射
するようにした生化学用試験装置において、 上記容器の筒部を上記マイクロ波によって発生する電界
の最小値の位置に設定するために、上記短絡板をその位
置が上記アプリケータ内において可変となるように構成
したことを特徴とする生化学用試験装置。1. An applicator provided with a short-circuit plate on one side, and a container formed of a tubular portion for covering the head of the biological specimen and a main body for covering the body portion, the tubular portion being provided in the applicator. In the biochemical test device which is made to project and irradiate the microwave from the direction opposite to the short-circuit plate, in order to set the tubular portion of the container to the position of the minimum value of the electric field generated by the microwave, A biochemical test device, characterized in that the position of the short-circuit plate is variable within the applicator.
ら内部に挿入されていることを特徴とする実用新案登録
請求の範囲第１項記載の生化学用試験装置。2. The biochemical test device according to claim 1, wherein the tubular portion is inserted into the applicator from a short piece side thereof.
波の管内波長の1/2又は1/2の整数倍になるようにしたこ
とを特徴とする実用新案登録請求の範囲第１項或いは第
２項記載の生化学用試験装置。3. A utility model registration claim characterized in that the distance between the short-circuit plate and the cylindrical portion is 1/2 or an integral multiple of 1/2 of the guide wavelength of the microwave. The test apparatus for biochemistry according to item 2 or item 2.
可能な電波洩れ防止の蓋を有することを特徴とする実用
新案登録請求の範囲第１項乃至第３項記載の生化学用試
験装置。4. The biochemical test according to any one of claims 1 to 3, wherein the main body of the container has a detachable lid for preventing radio wave leakage on an outer opening surface thereof. apparatus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5609386U JPH0637680Y2 (en) | 1986-04-16 | 1986-04-16 | Biochemistry test equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5609386U JPH0637680Y2 (en) | 1986-04-16 | 1986-04-16 | Biochemistry test equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62168464U JPS62168464U (en) | 1987-10-26 |
| JPH0637680Y2 true JPH0637680Y2 (en) | 1994-10-05 |
Family
ID=30884603
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5609386U Expired - Lifetime JPH0637680Y2 (en) | 1986-04-16 | 1986-04-16 | Biochemistry test equipment |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0637680Y2 (en) |
-
1986
- 1986-04-16 JP JP5609386U patent/JPH0637680Y2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62168464U (en) | 1987-10-26 |
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