JPH0650937A - Electrophoresis apparatus - Google Patents

Abstract

(57) [Summary] [Object] To provide an electrophoretic device having good separation performance by effectively removing air bubbles and foreign substances that have accumulated or adhered in an electrophoretic tank that adversely affects the flow of an internal solution. [Structure] Two pairs of solution inlets 21 and 21 'and outlets 22 and 22' are provided on the upper and lower side surfaces of the electrophoretic tank 1 so as to face each other.
These are connected to the solution pump 25 via the corresponding switching valves 27, 27 'and 28, 28' to form a pipe loop 26, and a bubble separator 24 and a foreign matter remover 34 are provided. The bubble separator 24 is a case whose pressure can be reduced by a vacuum pump 29.
It has a singing 30, a tube-shaped hydrophobic porous membrane 31, an inlet 32 and an outlet 33. The foreign matter remover 34 includes a filter 36 for removing foreign matter in the casing 35, a solution inlet 37, and a solution outlet 38. Also, a cleaning liquid supply means is provided. [Effects] Since bubbles and foreign substances that have accumulated or adhered in the migration tank can be separated by the solution flow and passed through the bubble separator and the foreign substance remover to be removed, an electrophoresis device with good separation performance is provided. sell.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an electrophoretic device used for analysis and separation / purification of biological samples, and more particularly to an electrophoretic device having good performance for removing air bubbles and foreign substances in an electrophoretic tank.

[0002]

2. Description of the Related Art Conventionally, an electrophoresis method has been indispensable for studying biological components having various functions, and has recently been spotlighted as a means for separating and purifying biological samples such as cells and proteins. Although various types of electrophoretic methods have been devised and used, the carrier-free electrophoretic method, which has attracted attention in particular, will be described. The principle of this electrophoretic method is a method of continuously injecting a sample into a separating electrolyte that flows as a liquid film in the form of a thin film in an electrophoretic tank and performing electrophoresis while moving as a laminar flow.

The apparatus is constructed in a migration tank having a rectangular cross section.
An electrolyte solution called a buffer solution is caused to flow in a laminar flow, and a DC voltage is applied to electrodes placed on both sides of the electrolyte solution to form an electric field in a direction perpendicular to the flow direction of the buffer solution. When a sample is injected into this buffer solution, each component in the sample receives a force corresponding to the surface charge of itself from the electric field and moves in the migration tank in the direction of the electric field. As a result, each sample component is separated to form a narrow separation zone, which is then separated by the separating means.

Further details will be described with reference to FIG. 5A is an explanatory view of the electrophoretic device, and FIG. 5B is a vertical cross-sectional view of the electrophoretic device of FIG. 5A. Figure 5
In (a) and (b), 1 is a migration tank for flowing a rectangular solution, 2, 2'is positive and negative electrodes, 3, 3'is an electrode chamber,
Numerals 4 and 4 ′ are a cation exchange membrane and an anion exchange membrane, and the positive and negative electrodes 2 and 2 ′ are provided on both side surfaces of the electrophoretic tank 1. The positive and negative electrodes 2 and 2 ′ are the electrode chamber 3 3 '
It is included in. The electrode chambers 3 and 3'are separated from the inside of the electrophoretic bath 1 by a cation exchange membrane 4 and an anion exchange membrane 4 ', respectively. A plurality of inlets 5 for introducing an electrolyte solution (hereinafter, referred to as a buffer solution) is provided in the upper part of the electrophoretic tank 1 and is connected to a tank 6 containing the buffer solution.

Reference numeral 7 is a preparative separation channel for separating the buffer solution, 8 is a multiple pump for sucking the separated buffer solution, and 9 is a preparative container for collecting the separated buffer solution. At the bottom of the migration tank 1,
As shown in the drawing, a large number of preparative separation channels 7 are arranged side by side in the left-right direction, and each pump element of the multiple preparative pump 8 is connected to each of the preparative separation channels 7 and continuously connected thereto. The sorting container 9 is connected.

Next, the function of this electrophoresis apparatus will be described. The buffer solution is dispensed into the corresponding dispensing container 9 by the action of the multiple dispensing pump 8. Due to this sorting action, a laminar flow of the buffer solution is formed in the migration tank 1 downward in the tank. The sample in the container 10 containing the sample is injected from the sample injection port 12 by operating the injection pump 11. The injected sample rides on the laminar flow of the buffer solution in the electrophoretic tank, and the positive and negative electrodes 2, 2 '
Is affected by the electric field formed by As a result, each sample component receives a force in the electric field direction according to its own surface charge and moves in the electric field direction of the migration tank 1, that is, in the left-right direction of FIG. As described above, each component is separated and collected in each collecting container 9.

Here, the electrophoretic device has a structure in which the bath wall on the long side of the rectangular section is cooled with cooling water or the like in order to remove the Jule heat generated by the applied voltage. 5 to prevent the effect of heat convection on the
In the vertical cross-sectional view of the migration tank 1 of (b), the thickness δ of the migration tank 1 is 1 mm or less on the ground, and even in outer space where it is said that the influence of thermal convection is small, it is at most several mm due to cooling restrictions. Limited within. In this case, if air bubbles or foreign matters are accumulated or adhered in the migration tank 1,
The flow of the buffer solution at a relatively low speed cannot flow to the multiple preparative pump 8 side. Therefore, it can be seen that the flow of the buffer solution around these bubbles or foreign matter becomes turbulent and adversely affects the separation performance. A known literature example of these circumstances "Yu Fujikura: New Development of Electrophoresis: BIO INDUSTR"
Y, vol. 2, No. 2, 1985 ".

[0008]

In the above-mentioned conventional electrophoretic device, the biggest obstacle to the formation of the laminar flow of the buffer solution in the electrophoretic tank having such a small thickness is the electrophoretic migration. The presence of foreign matter such as bubbles or dust that has accumulated in the tank or has adhered to it. Of these, air bubbles are mainly mixed and left when the buffer solution is initially introduced into the electrophoresis tank in a dry state, and these remain in the electrophoresis tank or adhere to the wall surface of the electrophoresis tank due to surface tension.

On the other hand, some foreign substances such as dust are contained in the buffer solution from the beginning, but mainly the sample residue such as cells is suspended in the migration tank or adheres to the wall surface. ing. Among the above, the bubbles adhered to the wall surface of the migration tank due to the surface tension are separated from the wall surface and cannot be discharged by the preparative pump due to the relatively slow flow of the buffer solution. Further, foreign matter such as dust existing in the migration tank cannot be removed by the preparative pump and remains because the pipe diameter of the preparative flow channel is extremely small.

In any case, when these air bubbles or foreign substances are present in the migration tank, a turbulent flow is formed around them, and a laminar flow of the buffer solution cannot be formed, resulting in poor separation performance. The present invention has been made to solve the above-mentioned problems of the prior art, and provides an electrophoretic device having good separation performance by effectively removing air bubbles and foreign matter accumulated or adhering to the above-mentioned migration tank. With the goal.

[0011]

In order to achieve the above-mentioned object, the structure of the present invention relating to an electrophoretic device includes a migration tank for flowing a solution, and a flow of the solution inside the migration tank. In an electrophoretic device comprising a preparative pump and positive and negative electrodes provided on both sides of the migration tank to form an electric field at right angles to the flow of the solution, at least one pair or more of the openings opened on the wall surface of the migration tank. A suction port and a discharge port for the solution are provided, and a pipe loop equipped with a solution pump is connected to the suction port and the discharge port, and a bubble separator is provided in the pipe loop for removing bubbles. Be prepared.

Another configuration of the present invention relating to the electrophoretic device is that in the above configuration, in order to remove foreign matter, a foreign matter remover is provided in the pipe loop instead of the bubble separator. Still another configuration of the present invention relating to the electrophoretic device is that in the above configuration, a bubble separator and a foreign matter remover are provided in the pipe loop for removing bubbles and foreign matter. Still another configuration of the present invention relating to the electrophoretic device is the above-mentioned configuration, which comprises a cleaning liquid for cleaning the wall surface in the pipe loop in order to remove air bubbles and foreign matter adhering to the wall surface in the migration tank. It is equipped with a supply means. Still another configuration of the present invention relating to the electrophoretic device is the above-mentioned configuration, in order to effectively remove air bubbles or foreign matters that have accumulated or adhered to the entire area of the migration tank, these suction ports are provided in the pipe loop. And a solution switching valve corresponding to each of the discharge port and the discharge port.

[0013]

The operation of each of the above technical means is as follows. According to the configuration of the present invention, at least a pair of inlets and outlets for a solution, which are opened on the wall surface in the migration tank, are provided, the inlets and outlets are connected, and a pipe loop equipped with a solution pump is provided. Since a bubble separator is provided in the solution, by operating this solution pump, it becomes possible to form a relatively strong flow of the solution from the discharge port of the solution to the suction port in the migration tank, and to stay in the migration tank. Alternatively, the attached bubbles can be put on the flow of this solution and sucked together with the solution from the suction port. Since only the bubbles of the bubble-mixed solution are removed by the bubble separator in the pipe loop, the bubble-free solution is supplied from the discharge port into the migration tank.

According to another aspect of the present invention, since the foreign matter remover is provided in the pipe loop, the solution pump in the pipe loop is operated to store the solution in the migration tank. A relatively strong flow of the solution from the discharge port to the suction port can be formed, and the foreign matter floating or adhering to the migration tank can be placed on the flow of the solution and sucked together with the solution from the suction port. Since only the foreign matter is removed by the foreign matter remover in the pipe loop, the solution containing the foreign matter is supplied from the discharge port into the migration tank with the foreign matter-free solution.

Further, according to still another structure of the present invention, since the bubble separator and the foreign matter remover are provided in the pipe loop, the relatively strong structure formed in the migration tank by the operation of the solution pump. By the action of the flow of the solution, the air bubbles and the foreign matter that have accumulated or adhered in the migration tank can be sucked together with the solution from the suction port. Since the bubble separator and the foreign matter remover in the pipe loop remove the bubbles and the foreign matter from the solution containing the bubbles and the foreign matter, only the solution is supplied from the discharge port into the migration tank.

Further, according to still another aspect of the present invention, since the cleaning liquid supply means is provided in the pipe loop, the cleaning liquid is supplied from the discharge port to the migration tank by using this means. Can be supplied to clean the wall surface of the migration tank and the inside of the pipe loop. Furthermore, according to the configuration of the present invention, in the pipe loop, at least a pair of suction ports and discharge ports provided on the wall surface of the migration tank are provided with a solution switching valve corresponding to each of them. Therefore, by combining the opening and closing of these switching valves, it is possible to form the flow of the solution in various directions from the discharge port to the suction port in the migration tank, and almost all the bubbles and foreign substances in the migration tank can be discharged from the suction port together with the solution. The solution can be removed by suction and passing the solution through the removing means in the pipe loop.

[0017]

Embodiments of the present invention will be described below with reference to FIGS. [Embodiment 1] FIG. 1 is an explanatory view of an electrophoretic device according to an embodiment of the present invention. The electrophoretic device of the present embodiment according to the present invention is provided with an electrophoretic tank for flowing a solution, a preparative pump for forming a flow of the solution inside the electrophoretic tank, and both sides of the electrophoretic tank. An electrophoretic device provided with positive and negative electrodes that form an electric field at right angles to the flow of the solution, and at least a pair of inlets and outlets for the solution opened on the wall surface of the electrophoretic tank, And a solution pump for pouring the solution in and out through the suction port and the discharge port, and further, a pipe loop for connecting the suction port, the discharge port and the solution pump is provided. It is configured with a bubble separator inside the loop.

In FIG. 1, 1 is an electrophoretic tank for flowing a rectangular solution (hereinafter, referred to as a buffer solution in the present embodiment), 2,
2'is a positive and negative electrode, 3,3 'is an electrode chamber, 4,4' is a cation exchange membrane and an anion exchange membrane, 5 is a plurality of inlets for guiding a buffer solution, and 6 is a buffer solution. A tank, 7 is a separation channel for separating the buffer solution, 8 is a multiple pump for sucking the separated buffer solution, 9 is a collecting container for collecting the separated buffer solution, 21 and 21 'are suction ports 21'. , 22 and 22 'are discharge ports, 23 is piping, 24 is a bubble separator, 25 is a solution pump, 26 is a piping loop, 27.27, '28 .28' are switching valves, 29 is a vacuum pump, 30 Is the casing of the bubble separator, 31 is the porous membrane in the bubble separator, and 32 and 33 are the inlet and outlet of the bubble separator.

The positive and negative electrodes 2, 2'are provided on both side surfaces of the electrophoretic tank 1, and the positive and negative electrodes 2, 2'are contained in the electrode chambers 3, 3 '. And this electrode chamber 3,
3'and the inside of the migration tank 1 are partitioned by a cation exchange membrane 4 and an anion exchange membrane 4 ', respectively. A plurality of inlets 5 for introducing a buffer solution, which is an electrolyte solution, is provided in the upper part of the electrophoretic tank 1, and is connected to a tank 6 containing the buffer solution. On the other hand, in the lower part of the migration tank 1, a large number of preparative separation channels 7 are provided side by side in the left-right direction in the figure, and the buffer solution is distributed into the corresponding preparative container 9 by the action of the multiple preparative separation pump 8. To be taken. Due to the action of the preparative pump 8, a laminar flow of the buffer solution is formed inside the migration tank 1 downward in the tank. Therefore, the sample in the sample container 10 is injected from the sample injection port 12 by operating the pump 11. The sample is subjected to the action of an electric field formed by the positive and negative electrodes 2, 2'while riding on the laminar flow of buffer solution in the migration tank. As a result, each sample component receives a lateral force corresponding to its own surface charge, moves in the left-right direction of the migration tank, is separated into each component, and is collected in each collecting container 9.

On the upper and lower side surfaces of the flow path of the buffer solution of the migration tank 1,
Two pairs of suction ports 21, a discharge port 22 and a suction port 2 facing each other
1'and a discharge port 22 'are provided, and these are connected to a bubble separator 24 and a solution pump 25 by a pipe 23 to form a pipe loop 26. The pipe 23 is provided with switching valves 27 and 27 'corresponding to the suction ports 21 and 21', and switching valves 28 and 28 'corresponding to the discharge ports 22 and 22'. Therefore, it is possible to combine an arbitrary suction port and discharge port with a combination of opening and closing of these switching valves. Here, the structure of the bubble separator 24 is such that a tube-like hydrophobic porous film 31 is provided in a casing 30 which can be decompressed by a vacuum pump 29, and an inlet 32 and an outlet 33 are provided.
Thus, a solution containing bubbles can pass through the porous film 31.

Next, the bubble removing operation of this embodiment will be described. It is carried out after introducing the buffer solution into the migration tank 1 and before actually performing the electrophoretic separation. First, by the action of the solution pump 25, the buffer solution is supplied into the migration tank from the discharge ports 22 and 22 'corresponding to the open valves of the switching valves 28 and 28', and at the same time, the switching valves 27 and 28 '. The buffer solution is sucked into the pipe loop 26 from the migration tank 1 through the suction ports 21 and 21 'corresponding to the open ones of the 27'. Therefore, in the migration tank 1, a flow of the buffer solution is formed leftward in FIG. 1, and the bubbles staying or adhering to the migration tank 1 ride on this flow and are sucked into the suction ports 21, 21 '.
Is sucked into the pipe loop 26 from.

Next, the bubble-containing buffer solution is introduced into the bubble separator 24 in the pipe loop 26. The hydrophobic porous membrane 31 of the bubble separator 24 has a function of allowing gas to pass therethrough but blocking passage of liquid. Therefore, the entrance 32
The introduced buffer solution containing bubbles flows in the porous film 31. Since the pressure outside the porous membrane 31 is reduced by the vacuum pump 29, a pressure difference is generated between the inside and outside of the membrane 31. Due to the pressure difference, only bubbles are discharged to the outside of the film 31 and sucked by the vacuum pump. The bubbles in the buffer solution reaching the outlet 33 can be relatively small or none. This buffer solution is again discharged into the migration tank 1 by the solution pump 25 through the discharge ports 22 and 22 'corresponding to the switching valves 28 and 28' which are in the open state. It should be noted that the bubbles discharged into the migration tank 1 that could not be removed by only passing through the bubble separator 24 once again were sucked into the suction port 2 again.
It is sucked from 1, 21 'and supplied to the bubble separator 24 in the pipe loop 26. By repeating this operation many times, it can be finally removed.

In this case, the solution pump 25 can have a relatively large capacity. In addition, the diameter of the pipe 23 is also the sorting flow path 7.
You can use a thicker one. Therefore, the discharge port 2
The flow of the leftward buffer solution formed in the migration tank 1 by being discharged from 2, 22 'and being sucked in by the suction ports 21, 21' is the downward flow formed by the above-described preparative pump 8 during migration. The flow rate can be higher and the force can be stronger than the flow. For this reason, the air bubbles accumulated or adhered in the migration tank 1 are put on the flow, and the suction port 2 is effectively used.
It is possible to inhale from No. 1 and No. 21 'and to use for a bubble separator.

On the other hand, the suction ports 21, 2 provided in the pipe 23
Depending on the combination of opening and closing of the switching valves 27, 27 'on the 1'side and the switching valves 28, 28' on the discharge ports 22, 22 'side, only the flow of the buffer solution in the electrophoretic tank 1 is directed to the side directly shown by the solid line. Instead, it is possible to form an obliquely upward or obliquely downward flow indicated by the broken line. Therefore, the bubbles that have accumulated or adhered to almost the entire area of the migration tank 1 are separated,
The bubble mixing buffer can be removed by passing it through the bubble separator 24. Further, the operation of removing bubbles may be performed until all the bubbles in the migration tank 1 can be removed. As described above, according to this embodiment, the bubbles that have accumulated or adhered to almost the entire area of the migration tank 1 can be effectively removed.

[Second Embodiment] Next, another embodiment of the present invention will be described. FIG. 2 is an explanatory diagram of an electrophoretic device according to another embodiment of the present invention. In the figure, the same reference numerals as those in FIG. 1 are the same parts, and the description thereof will be omitted. Further, the buffer solution inlet 5, the buffer solution tank 6 and its piping system, the sample container 10, the sample pump 11 and its piping system are not directly related to the operation of this embodiment, and are therefore omitted from the drawing. In the embodiment shown in FIG. 2, the pipe loop 2 shown in [Embodiment 1] is used.
6, a foreign matter remover 34 is provided in place of the bubble separator 24. The foreign matter remover 34 has a casing 35.
And a filter 36 for removing foreign matter is provided therein, and the solution is configured to flow in and out through the inlet 37 and the outlet 38.

Next, the operation of this embodiment will be described. The foreign matter removing operation is also carried out each time it is necessary before the actual electrophoretic separation, as in [Example 1]. First, due to the action of the solution pump 25, a flow of the buffer solution toward the left is formed in the electrophoretic tank 1 as in the case of [Example 1], and the foreign matter floating or adhering to the electrophoretic tank 1 is Riding the flow, suction port 2
It is sucked into the pipe loop 26 by 1, 21 '. In the pipe loop 26, the solution containing the foreign matter is removed by the foreign matter remover 34.
The foreign matter removing filter 36 removes the foreign matter, and the solution from which the foreign matter is removed flows out from the outlet 38, and the solution pump 25 works to discharge
It is again discharged into the migration tank 1 from 22 '.

Also in this embodiment, for the same reason as described in [Embodiment 1], the flow to the left formed in the electrophoretic tank 1 has a relatively large flow rate and strong momentum. You can Therefore, it is possible to effectively remove the foreign matter floating or adhering to the migration tank 1 by putting it on the flow. In addition, as described in [Example 1], the switching valves 27, 27 ', 28.
By the operation 8 ', since the flow of the buffer solution in various directions can be formed in the migration tank 1, it is possible to target almost all the foreign matters in the migration tank 1. As described above, according to the present embodiment, it is possible to effectively remove the foreign matter floating or adhering to almost the entire area of the migration tank 1.

[Embodiment 3] FIG. 3 is an explanatory diagram of an electrophoresis apparatus according to still another embodiment of the present invention. In the figure, the same reference numerals as those in FIG. 1 are the same parts, and the description thereof will be omitted. Further, the buffer solution inlet 5, the buffer solution tank 6 and its piping system, the sample container 10, the sample pump 11 and its piping system are not directly related to the operation of this embodiment, and are therefore omitted from the drawing. In the electrophoretic device shown in FIG.
In order to remove both the air bubbles and the foreign matter accumulated or adhered inside, both the foreign matter remover 34 and the air bubble separator 24 are provided in the pipe loop 26. Since the configurations of the foreign matter remover 34 and the bubble separator 24 are the same as those described in the above [Embodiment 1] and [Embodiment 2], the description thereof will be omitted.

Therefore, in the operation of the apparatus in which the foreign matter remover 34 and the bubble separator 24 are combined, first, the action of the solution pump 25 causes the inside of the migration tank 1 to carry out the above-mentioned [Example 1] and [Example 2]. A flow to the left is formed similarly to the above, and the air bubbles and foreign substances that have accumulated or adhered in the migration tank 1 are sucked into the pipe loop 26 through the suction ports 21 and 21 'along with this flow. The buffer solution containing air bubbles and foreign matter is first removed from the foreign matter remover 3 in the pipe loop 26.
4 and the foreign matter is removed by the foreign matter removing filter-36. After that, the bubbles are removed by the bubble separator 24, and only the buffer solution is removed by the action of the solution pump 25.
It is again discharged into the migration tank 1 through the discharge ports 22 and 22 '.

Also in this embodiment, the
1] and [Embodiment 2], the leftward flow formed in the migration tank 1 can have a relatively large flow rate and strong power. For this reason, it is possible to effectively remove the bubbles and the foreign matters that have accumulated or adhered in the migration tank 1 by putting them on the flow. Further, in the same manner as described in the above [Example 1] and [Example 2],
Suction side and discharge side switching valves 27, 27 ', 28, 2
Since the flow of liquid in various directions can be formed in the migration tank 1 by the combination of the opening and closing of 8 ', it is possible to target the bubbles and foreign substances in almost all areas in the migration tank 1. As described above, according to the present embodiment, it is possible to effectively remove both the air bubbles and the foreign matter that have accumulated or adhered to almost the entire area of the migration tank 1.

[Embodiment 4] FIG. 4 is an explanatory view of an electrophoresis apparatus according to still another embodiment of the present invention. In the figure, the same reference numerals as those in FIG. 1 are the same parts, and the description thereof will be omitted. Further, the buffer solution inlet 5, the buffer solution tank 6 and its piping system, the sample container 10, the sample pump 11 and its piping system are not directly related to the operation of this embodiment, and are therefore omitted from the drawing. In this embodiment, the inside of the migration tank 1 and the inside of each element of the piping loop 26 are provided in the piping loop 26 described in the above [Example 1], [Example 2], and [Example 3]. A cleaning liquid injecting section 39 is additionally provided in order to facilitate cleaning and cleaning of foreign substances adhering to these wall surfaces. As the cleaning liquid injected by the cleaning liquid injecting section 39, it is preferable to use a surfactant. According to this embodiment,
In addition to simply removing air bubbles and foreign matter in the migration tank 1,
A new effect that the inside of the migration tank 1 and the inside of the pipe loop 26 can be washed can be expected. The present invention relates to the above-mentioned [Example 1],
The application is not limited only to [Embodiment 2], [Embodiment 3] and [Embodiment 4], and the following modifications and applications can be considered. First, the positions and numbers of the inlets and outlets of the solution attached to the electrophoresis tank and the switching valves are to be selected as appropriate depending on the shape of the electrophoresis tank and the direction of the solution flow to be formed in the electrophoresis tank. You can Further, the positional relationship among the cleaning liquid supply unit, the foreign matter remover, the bubble separator, and the solution pump in the pipe loop is the above order along the flow of the solution in the above-mentioned embodiment, but the positional relationship is appropriately changed. It is possible. Also, a plurality of solution pumps may be provided.

As described above in detail, according to the present invention, air bubbles and foreign matters that have accumulated or adhered in the migration tank are
Since it can be removed by the bubble separator and the foreign matter remover in the pipe loop, it is possible to provide an electrophoretic device with good separation performance.

[Brief description of drawings]

FIG. 1 is a plan view of an electrophoretic device according to an embodiment of the present invention.

FIG. 2 is an explanatory diagram of an electrophoretic device according to another embodiment of the present invention.

FIG. 3 is an explanatory diagram of an electrophoretic device according to still another embodiment of the present invention.

FIG. 4 is an explanatory diagram of an electrophoretic device according to still another embodiment of the present invention.

FIG. 5 is an explanatory view of an electrophoretic device according to a conventional technique and a longitudinal sectional view thereof.

[Explanation of symbols]

 1 Electrophoresis tank 2, 2'electrode 3, 3'electrode chamber 4, 4'ion exchange membrane 5 buffer solution inlet 6 buffer solution tank 7 preparative flow path 8 multiple preparative pump 9 preparative container 10 sample container 11 sample Pump 12 Sample injection port 21,21 'Suction port 22, 22' Discharge port 23 Piping 24 Bubble separator 25 Solution pump 26 Piping loop 27, 27 'Switching valve 28, 28' Switching valve 29 Vacuum pump 30 Casing 31 Porous Membrane 32 Inlet 33 Outlet 34 Foreign Material Remover 35 Casing 36 Filter-37 Inlet 38 Outlet 39 Cleaning Solution Supply Section

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Yoshio Kojima, 216 Kintatemachi, Tsuchiura-shi, Ibaraki Prefecture, Hitate Works Co., Ltd. (72) Tsutomu Okusawa, 216 Kintatecho, Tsuchiura-shi, Ibaraki, Nitate Works Co., Ltd. Machinery Research Institute (72) Inventor Kuniyoshi Tsubouchi 216 Kandamachi, Tsuchiura City, Ibaraki Prefecture

Claims (5)

[Claims] 1. An electrophoretic tank for flowing a solution, a preparative pump for forming a flow of the solution inside the electrophoretic tank, and an electric field provided on both sides of the electrophoretic tank and perpendicular to the flow of the solution. In the electrophoretic device provided with positive and negative electrodes for forming a solution, at least one pair of inlets and outlets of the solution opened on the wall surface of the electrophoretic tank are provided, and the inlet and outlet are connected, and the solution circulation is performed. An electrophoretic device characterized in that a pipe loop provided with a pump is provided and a bubble separator is arranged in the pipe loop. 2. An electrophoretic tank for flowing a solution, a preparative pump for forming a flow of the solution inside the electrophoretic tank, and an electric field perpendicular to the flow of the solution, which is provided on both sides of the electrophoretic tank. In the electrophoretic device provided with positive and negative electrodes for forming a solution, at least one pair of inlets and outlets of the solution opened on the wall surface of the electrophoretic tank are provided, and the inlet and outlet are connected, and the solution circulation is performed. An electrophoretic device characterized in that a pipe loop equipped with a pump is provided, and a foreign matter remover is arranged in the pipe loop. 3. An electrophoretic tank for flowing a solution, a preparative pump for forming a flow of the solution inside the electrophoretic tank, and an electric field perpendicular to the flow of the solution, which is provided on both sides of the electrophoretic tank. In the electrophoretic device provided with positive and negative electrodes for forming a solution, at least one pair of inlets and outlets of the solution opened on the wall surface of the electrophoretic tank are provided, and the inlet and outlet are connected, and the solution circulation is performed. An electrophoretic device characterized in that a pipe loop equipped with a pump is provided, and a bubble separator and a foreign matter remover are arranged in the pipe loop. 4. A pipe loop connected to a suction port and a discharge port and provided with a solution circulation pump, wherein a cleaning liquid supply means is provided in the loop. , 3
An electrophoretic device according to any of the claims. 5. A pipe loop connected to a suction port and a discharge port and equipped with a solution circulation pump, provided with a solution switching valve corresponding to the suction port and the discharge port in the loop. The electrophoretic device according to claim 1, 2, 3, or 4.