JPH0680502A - Preparation for specimen usable for internal transparent - Google Patents

Abstract

PURPOSE: To provide a process for producing a see-through specimen which preserves the whole body or necessary part of the living body in particular transparently into a specimen bottle and making it possible to finely observe the fine cellar tissues and internal structures, such as distribution state, of blood vessels of the living body without being restrained in a visual direction. CONSTITUTION: This process consists of stags for immersing and fixing the living body into 7 to 12% Formalin, then injecting the dyestuff of saffranine and methylene blue therein, subjecting the living body to a bleaching treatment with 7 to 12% pernitric acid or 3 to 7% hydrogen peroxide, dehydrating the living body with 100% alcohol in succession thereto, putting the living body into a specimen container of wintergreen (sweet-birch) oil which is a transparent agent in the state of subjecting the living body to a spirits-of-vine treatment with benzene and sealing the container by application of a silicone resin adhesive ('Mendein (R)').

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a biological specimen capable of internal see-through, and in particular, the whole body of an organism or a necessary part of the organism is transparently preserved in a specimen bottle to obtain fine cell tissues and blood vessels of the organism. The present invention relates to a method for producing a fluoroscopic specimen, which enables detailed observation of the internal structure such as the distribution state of the above without being constrained in the visual direction.

[0002]

2. Description of the Related Art Generally, all specimens of organisms provided generally focus on long-term preservation methods. Although it can be stored for a while, the distribution of the cellular tissues and blood vessels forming the organism could not be observed because the interior of the specimen was opaque.

Therefore, in the case of animals, necessary parts must be dissected one by one, and in the case of plants, they are cut off by a special construction method and treated with a special drug to give the blood vessel distribution state of the animals. There was a drawback with the complexity of having to observe the structure of the cellular tissue state of animals and plants.

[0004] Therefore, the conventional sample is in the actual state which does not fall under the category of the simple sample used for display and learning for the lower grades.

The present invention has been devised in order to solve the above-mentioned various problems of the related art, and is a fluoroscope for learning that allows easy observation of internal organs and the like without dissecting a living organism. The main purpose is to provide a sample.

[0006] Another object of the present invention is to provide a fluoroscopic specimen which can be selectively used for specific research by selectively coloring the blood vessel distribution and other tissues of an organism.

[0007]

[Means for Solving the Problems] In order to achieve the above object, the present invention comprises immersing an organism in 7 to 12% formalin to fix the organism, and then adding safranin and methylene blue pigments. Inject, 7-1
Bleach with 2% pernitric acid or 3-7% hydrogen peroxide and continue to dehydrate with 100% alcohol before
It is characterized in that it is put in a sample container containing wintergreen oil, which is a transparent agent, after being treated with benzene and then applied and sealed with a silicone resin adhesive (Mendin).

[0008]

In the present invention, the fluoroscopic specimen means that a living body was put in a preservative solution and sealed in a container-shaped glass bottle like a normal specimen.
Since the cell tissue of an organism can be seen transparently, it can be seen as if it was irradiated with X-rays.

If the X-ray photograph is flat, on the contrary, the fluoroscopic specimen is three-dimensional because the state of the internal structure of the living body can be seen in detail and clearly from a certain direction.

The principle of the fluoroscopic specimen of the present invention is that the tissue of each part of a living body has a different refractive index of each light beam, so that the living body is immersed in a liquid having a refractive index close to its average refractive index. When the organism is inserted into this liquid, the organism is transparently seen through.

Applications of the above theory are in contact with each other 2
When a material has a significantly different refractive index, the light ray immediately reflects from the contact surface of the material and does not immediately pass through the material. Is that the ray does not pass through the organism.

The present inventor has completed the present invention by discovering a transparent body that can satisfy the purpose of the present invention as a result of studying with the above points in mind.

[0013]

[Examples] A method for producing a fluoroscopic specimen will be described below with reference to an example of a fish, which is a living body.

A fish organism was fixed in 10% formalin, and safranin and methylene blue (Meth) were fixed.
ylene blue) into the artery and vein respectively to inject the dye, bleach with 10% pernitric acid or 5% hydrogen peroxide and dehydrate with 100% alcohol before using benzene. Remove the spirits and put it in a transparent container filled with winter green oil, which is a transparent agent, and apply a silicone resin adhesive {Mendin (trade name is compatible)} to seal it.

The method for producing a biological fluoroscopic specimen of the present invention, taking the fish as an example, will be described in more detail.

Before bleaching, it is necessary to immerse and fix the specimen in a 10-fold formalin solution. If this is not done, hydrogen peroxide will be instantly decomposed due to the oxygen contained in the organism.

The injection is preferably carried out when the animal is alive, and when possible, it is preferably carried out when the heartbeat is stopped, as is the case at the time of dissection, or when the heartbeat is about to stop. . When dissecting and storing the residue as a fluoroscopic specimen, the main color is injected into the local artery.

Colloid is mixed in the main color, but of course, the material at this time must be placed in a sufficiently warm bath before injection. The injection specimen is good for observing the distribution of blood vessels such as the heart, lung, stomach, small intestine, and kidney.

Since bleaching is the most important factor in producing the sample in question, if any color remains on the sample and is not completely bleached, it becomes an obstacle and the interior is not transparent.

As the bleaching agent, pernitric acid or hydrogen peroxide is used, but since nitric acid and hydrogen peroxide are usually acidic,
It must not be forgotten to add a small amount of aqueous ammonia to neutralize this when using it. If used in an acidic state, the skeleton and other tissues will be eroded due to the acidic nature.

The period required for bleaching depends on the type of sample, but it is usually 2 weeks or more, and it is advisable to add a small amount of formalin to the relevant solution to prevent spoilage of the sample during bleaching.

For mammals and birds, first, cow hair is removed and immersed in formalin. After fully bleached specimens are taken out and repaired, they are tied to the glass plate surface, tidied up in the attached posture, dehydrated with alcohol, and then gradually diluted to pure sake.

Next, the refined liquor is removed. At this time, it is advisable to use benzene and replace it twice here. This is put in a sample bottle and winter green oil as a transparent agent is put therein, and then air or benzene in the sample is sucked by a vacuum pump or the like.

Through this process, although there are differences depending on the type of sample, it usually takes one month or more to complete a fluoroscopic sample.

A well-polished hemp glass is attached to the inlet of the sample storage bottle and sealed with a silicone resin adhesive (Mendin).

Since the fluoroscopic specimen prepared by the manufacturing method of the present invention can preserve the whole body of a small organism or a part of an animal body in its original shape as it is, it can be preserved transparently without dissecting the animal. It is possible to clearly observe the distribution state and the structure of fine cell tissues in a three-dimensional manner without being constrained by the visual position. It enables three-dimensionally detailed and vivid observation without the need to add a special drug for observation.The first feature of the present invention is that the internal structure of an opaque organism is three-dimensional. That can be observed transparently and clearly, secondly, that the distribution of cartilage skeleton such as fish can be observed completely clearly, and thirdly,
It is possible to clearly observe the distribution of blood vessels in the injection specimen, and fourthly, in the case of plants, it is possible to clearly predict the distribution state in the flow tube.

For example, when injected into the navel of a fetus such as cow or pig, it is possible to clearly observe the distribution state of these blood vessels, not to mention the blood vessel distribution in weak bones of infant animals.
The appearance of teeth in the alveolar space and the skull of a chicken are cut in half and made transparent, so that the trisection organ and the cochlea can be clearly observed.

The objects to be carried out by the method for producing a fluoroscopic specimen of the present invention are as follows, but the invention is not limited thereto.

[Iso] kimono, octopus, squid, cochlea, scorpion, sea star,
Invertebrates such as big shrimp, hoya, sea mussels, sea moon, korogi, roundworm, worms etc .: As vertebrates, fish such as Makoi, beech, beef tongue fish, karei, etc .: Hikikae, lizard, frog, etc. Amphibians: Reptiles such as poisonous snakes: Birds such as chickens and sparrows: rabbits, cats,
Fluoroscopic specimens of organisms were manufactured as subjects for conducting mammals such as dogs.

Although the organ system of each of the above-mentioned animals was also subject to the separate implementation, here, the heart, pancreas, eyeball, lung, stomach, skull, liver, nerve, anterior and posterior legs, kidney and internal organs were used.

On the other hand, various trachea of plants in the flow tube were targeted, but in this case, phoenix flowers, dahlia, corn, amber, radish, etc. were targeted.

[0032]

INDUSTRIAL APPLICABILITY As described above, according to the present invention, the external shape of an organism and the distribution state of whole or a part of cellular tissues and blood vessels are three-dimensionally detailed without being obscured in the visual direction. Since it can be observed clearly, it not only contributes to school education related to organisms, but also contributes to research materials in various fields such as biology, medicine, and agriculture, and is expected to be used for industrial display. To be done.

 ─────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Jun Narita ▲ Tetsu ▼ 2-34-21 Funato, Itabashi-ku, Tokyo Pegasus Mansion 602

Claims (1)

[Claims] 1. An organism is immersed in 7-12% formalin to fix it, and then safranin and methylene blue dyes are injected, and bleached with 7-12% pernitric acid or 3-7% hydrogen peroxide. Treated, continuously dehydrated with 100% alcohol, put in a specimen container containing winter green oil as a clarifying agent in a state of being treated with alcohol with benzene, and then coated with silicone resin adhesive (Mendin) A method for manufacturing a specimen capable of seeing through an organism, which is characterized by encapsulating.