JPH07155173A - Microbe exhibiting degradative prformance for polyoxyethylene nonyl phenyl ether - Google Patents
Microbe exhibiting degradative prformance for polyoxyethylene nonyl phenyl etherInfo
- Publication number
- JPH07155173A JPH07155173A JP33953493A JP33953493A JPH07155173A JP H07155173 A JPH07155173 A JP H07155173A JP 33953493 A JP33953493 A JP 33953493A JP 33953493 A JP33953493 A JP 33953493A JP H07155173 A JPH07155173 A JP H07155173A
- Authority
- JP
- Japan
- Prior art keywords
- ape
- strain
- present
- concentration
- treatment system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- -1 polyoxyethylene nonyl phenyl ether Polymers 0.000 title claims abstract description 9
- 230000003413 degradative effect Effects 0.000 title abstract 2
- 230000001747 exhibiting effect Effects 0.000 title 1
- 241000894006 Bacteria Species 0.000 claims description 16
- 244000005700 microbiome Species 0.000 claims description 12
- 238000004065 wastewater treatment Methods 0.000 claims description 9
- 241000589516 Pseudomonas Species 0.000 claims description 6
- 238000005516 engineering process Methods 0.000 claims description 4
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 3
- USIUVYZYUHIAEV-UHFFFAOYSA-N diphenyl ether Chemical compound C=1C=CC=CC=1OC1=CC=CC=C1 USIUVYZYUHIAEV-UHFFFAOYSA-N 0.000 claims 1
- 125000001400 nonyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
- 241000589774 Pseudomonas sp. Species 0.000 abstract description 4
- 239000010802 sludge Substances 0.000 description 19
- 238000012360 testing method Methods 0.000 description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 239000004094 surface-active agent Substances 0.000 description 12
- 239000002609 medium Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 238000000354 decomposition reaction Methods 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 239000002351 wastewater Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 125000000129 anionic group Chemical group 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000010840 domestic wastewater Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000002736 nonionic surfactant Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000000721 bacterilogical effect Effects 0.000 description 2
- 238000006065 biodegradation reaction Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000010842 industrial wastewater Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000013028 medium composition Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 235000002639 sodium chloride Nutrition 0.000 description 2
- 239000000271 synthetic detergent Substances 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000013382 Gelatinases Human genes 0.000 description 1
- 108010026132 Gelatinases Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000003945 anionic surfactant Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008233 hard water Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 235000011147 magnesium chloride Nutrition 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000028070 sporulation Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000019086 sulfide ion homeostasis Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、家庭用合成洗剤あるい
は工業用界面活性剤として使用されているポリオキシエ
チレンノニルフェニルエーテル（以下、ＡＰＥという）
を分解する能力を有する微生物に関するものであり、こ
の微生物を利用してＡＰＥを含有する生活排水及び産業
排水を浄化する方法に関するものである。FIELD OF THE INVENTION The present invention relates to polyoxyethylene nonyl phenyl ether (hereinafter referred to as APE) used as a household synthetic detergent or an industrial surfactant.
The present invention relates to a microorganism capable of decomposing water, and to a method for purifying domestic and industrial wastewater containing APE by using this microorganism.
【０００２】[0002]
【従来の技術】近年、界面活性剤は公衆衛生面の向上の
ための洗浄剤としてだけではなく、工業分野でも乳化剤
や湿潤剤として使用されるようになり、現代社会にとっ
て不可欠の化学物質となっており、工業発展とともに多
種多様な界面活性剤が作り出され使用されている。ポリ
オキシエチレン型の界面活性剤は、耐酸性・耐アルカリ
性・耐硬水性が高く、低濃度の使用であっても充分な界
面活性を示すことより、様々な分野で利用されている。
このポリオキシエチレン型の界面活性剤の中でもＡＰＥ
は、使用量が最も多い界面活性剤の一つである。2. Description of the Related Art In recent years, surfactants have come to be used not only as detergents for improving public health but also as emulsifiers and wetting agents in the industrial field, and become chemical substances indispensable to modern society. Therefore, a wide variety of surfactants have been created and used with industrial development. Polyoxyethylene type surfactants are used in various fields because they have high acid resistance, alkali resistance, and hard water resistance, and exhibit sufficient surface activity even when used at low concentrations.
Among these polyoxyethylene type surfactants, APE
Is one of the most used surfactants.
【０００３】[0003]
【発明が解決しようとする課題】界面活性剤が混入して
いる排水の処理は、一般に活性汚泥を用いて処理されて
いる。しかしながら、陰イオン界面活性剤である直鎖ア
ルキルベンゼンスルホン酸塩（ＬＡＳ）及び非イオン界
面活性剤であるＡＰＥなどは微生物による分解を受けに
くく、分解されずに排水処理水中に残った状態で放出さ
れる。また、このような界面活性剤により長期間かけて
活性汚泥を馴養培養しても分解能力を呈するようになる
のはごくわずかしかなく、その分解能力を得るまでに要
する馴養時間は長いので、この馴養培養方法による生分
解作用の向上はほとんど期待できない。DISCLOSURE OF INVENTION Problems to be Solved by the Invention Treatment of wastewater containing a surfactant is generally carried out using activated sludge. However, linear alkylbenzene sulfonate (LAS), which is an anionic surfactant, and APE, which is a nonionic surfactant, are not easily decomposed by microorganisms and are released without being decomposed in the wastewater treatment water. It Further, even if the activated sludge is acclimated and cultured for a long period of time with such a surfactant, it exhibits only a small amount of decomposition ability, and the acclimatization time required to obtain the decomposition ability is long. The improvement of biodegradation by the acclimation culture method can hardly be expected.
【０００４】[0004]
【課題を解決するための手段】このような事情に鑑み、
本発明者等はＡＰＥに対し、単独で優れた生分解性を示
す微生物を広く自然界に求め、種々の試験を繰り返した
結果、ＡＰＥに対して分解能力を持つシュードモナス
（Pseudomonas)に属するある種の細菌を見い出し、本発
明を完成した。[Means for Solving the Problems] In view of such circumstances,
The inventors of the present invention have widely sought, in nature, a microorganism that exhibits excellent biodegradability by itself against APE, and as a result of repeating various tests, a certain type of Pseudomonas belonging to Pseudomonas which has a degrading ability to APE. The bacterium was found and the present invention was completed.
【０００５】即ち、本発明のＡＰＥに対して分解性能を
示す微生物は、シュウドモナス・エスピー（Pseudomona
s sp.)ＡＰＥ６株として、工業技術院生命工学工業技術
研究所に平成５年９月２２日付けで寄託されているもの
で（工業技術院生命工学工業技術研究所寄託菌、受託番
号ＦＥＲＭ Ｐ−１３８７０。以下、ＦＥＲＭ Ｐ−１
３８７０という）、その細菌学的性質は以下のとおりで
ある。That is, the microorganisms showing the decomposing ability for the APE of the present invention are Pseudomona sp.
s sp.) APE6 strain that has been deposited at the Institute of Biotechnology, Institute of Industrial Science and Technology as of September 22, 1993 (Deposited Bacteria of Institute of Life Science and Technology, Institute of Industrial Science, accession number FERM P -13870. Hereinafter, FERM P-1
3870), and its bacteriological properties are as follows.
【０００６】（Ａ）形態的性質 ＣＧＹ寒天培地上で３０℃の温度で２４時間培養したと
き、以下の形態的特徴が観察される。 １）細胞形態 ：単かん菌 ２）グラム染色性 ：陰 性 ３）胞子形成能 ：な し ４）運動性 ：あ り(A) Morphological properties The following morphological characteristics are observed when culturing on a CGY agar medium at a temperature of 30 ° C. for 24 hours. 1) Cell morphology: Monobacillus 2) Gram stainability: Anonymous 3) Sporulation ability: None 4) Motility: Ari
【０００７】（Ｂ）培養的性質 １）ＣＧＹ寒天平板培養 コロニーの色 ：乳白色 コロニーの透明度 ：なし コロニーの光沢 ：あり 表面の形態 ：なめらか 隆起状態 ：やや隆起(B) Culture property 1) CGY agar plate culture Color of colony: milky white Clarity of colony: None Gloss of colony: Yes Surface morphology: Smooth raised state: Slightly raised
【０００８】（Ｃ）生理的性質 １）硝酸の還元 ：陽 性 ２）脱窒反応 ：陰 性 ３）ＭＲテスト ：陰 性 ４）ＶＰテスト ：陰 性 ５）インドールの生成 ：陰 性 ６）硫化水素の生成 ：陰 性 ７）デンプンの加水分解：陰 性 ８）クエン酸の利用 ：陽 性 ９）色素の生成 ：陰 性 １０）ゼラチナーゼ ：陽 性 １１）ウレアーゼ ：陰 性 １２）オキシターゼ ：陽 性 １３）カタラーゼ ：陽 性 １４）生育の範囲 ｐＨ ：５〜９ １５） 温度 ：１５〜３５℃ 普通ブイヨンにおける生育：良好 １６）酸素に対する態度 ：好気性 １７）Ｏ−Ｆテスト ：酸化的 １８）糖類からの酸及びガスの生成：表１に示すとおり(C) Physiological properties 1) Reduction of nitric acid: positive 2) Denitrification reaction: negative 3) MR test: negative 4) VP test: negative 5) Indole formation: negative 6) Sulfide Generation of hydrogen: Anionic 7) Hydrolysis of starch: Anionic 8) Utilization of citric acid: Cationic 9) Formation of pigment: Anionic 10) Gelatinase: Catalytic 11) Urease: Catalytic 12) Oxidase: Catalytic. 13) Catalase: Positive 14) Range of growth pH: 5 to 9 15) Temperature: 15 to 35 ° C Growth in normal broth: Good 16) Attitude toward oxygen: Aerobic 17) OF test: Oxidative 18) Sugar Generation of acids and gases from: As shown in Table 1.
【０００９】[0009]
【表１】 [Table 1]
【００１０】前記細菌学的性質の試験は、「バージーズ
・マニュアル・オブ・システマテイック・バクテリオロ
ジー」（Bergey's Manual of Systematic Bacteriolog
y）及び長谷川武治著「微生物の分類と同定」（学会出
版センター刊）に記載されている方法あるいは培地組成
により行った。これらの試験結果から、本発明の微生物
はシュウードモナス（Pseudomonas)属に属する細菌であ
ることが判った。The above-mentioned test of bacteriological properties is carried out by "Bergey's Manual of Systematic Bacteriolog".
y) and Takeshi Hasegawa, "Classification and Identification of Microorganisms" (published by the Society Publishing Center) or the medium composition. From these test results, it was found that the microorganism of the present invention is a bacterium belonging to the genus Pseudomonas.
【００１１】本発明の実施において、処理系におけるＡ
ＰＥの濃度を減少させるためには、燐酸一水素カリウ
ム、燐酸二水素カリウム、塩化カルシウム、塩化マグネ
シウム、塩化ナトリウム及び塩化鉄などを併用すること
により、より効率よくＡＰＥの濃度減少を促進させるこ
とができる。また、必要に応じて処理系に酵母エキス等
の有機微量栄養素を添加することにより、菌の増殖を促
進することもできる。In the practice of the present invention, A in the processing system
In order to reduce the concentration of PE, the combined use of potassium monohydrogen phosphate, potassium dihydrogen phosphate, calcium chloride, magnesium chloride, sodium chloride, iron chloride, etc. can promote the reduction of APE concentration more efficiently. it can. In addition, if necessary, organic micronutrients such as yeast extract may be added to the treatment system to promote the growth of bacteria.
【００１２】本発明の実施において本発明細菌を処理系
に組み込む方法としては、培養菌体をそのまま接種する
方法、乾燥菌体または乾燥製剤にして用いる方法及び細
菌を担体担持または包括固定して用いる方法がある。本
発明の実施において、本発明細菌の培養温度は２８℃程
度が好適であり、また処理系のｐＨは６〜８の範囲であ
り、特にｐＨ７．２程度が好適である。In the practice of the present invention, the method of incorporating the bacterium of the present invention into a treatment system includes a method of inoculating cultured cells as it is, a method of using dried cells or a dry preparation, and a method of supporting or entrapping bacteria in a carrier. There is a way. In the practice of the present invention, the culturing temperature of the bacterium of the present invention is preferably about 28 ° C., and the pH of the treatment system is in the range of 6 to 8, and particularly preferably about pH 7.2.
【００１３】[0013]
【作用】本発明の細菌が有する酵素等の作用機構につい
ては、現在のところ不明であるが、本細菌がＡＰＥを分
解する能力に優れていることは確認された。従って、本
細菌をＡＰＥを含む排水処理系あるいは河川水中に添加
することにより、ＡＰＥを効率良く分解し除去すること
ができる。The mechanism of action of the enzyme and the like possessed by the bacterium of the present invention is unknown at present, but it was confirmed that the bacterium has an excellent ability to decompose APE. Therefore, APE can be efficiently decomposed and removed by adding the bacterium to a wastewater treatment system containing APE or river water.
【００１４】[0014]
【実施例】以下、本発明を実施例及び比較例によって具
体的に説明する。なお、これらの試験において、ＡＰＥ
を定量する方法としては、ＪＩＳ Ｋ３３６２「合成洗
剤の生分解試験方法」に準拠したテトラチオシアノコバ
ルト酸吸光光度法（ＣＴＡＳ法）、つまり非イオン界面
活性剤とテトラシアノコバルト酸アンモニウムとの錯体
をベンゼンで抽出して、紫外部の吸光度（３２２ｎｍ）
を測定する方法により行った。EXAMPLES The present invention will be specifically described below with reference to Examples and Comparative Examples. In these tests, APE
As a method for quantifying, the tetrathiocyanocobaltate absorptiometry (CTAS method) based on JIS K3362 “Biodegradation test method for synthetic detergents”, that is, a complex of a nonionic surfactant and ammonium tetracyanocobaltate is used. Extracted with benzene, UV absorbance (322nm)
Was measured.
【００１５】（実施例１）表２に示す所定量のＡＰＥを
肉エキス１００ｍｇ、Ｋ2 ＨＰＯ4 ５００ｍｇ、ＮＨ4
Ｈ2 ＰＯ4 ６６０ｍｇ、ＮａＣｌ２５ｍｇ、ＣａＣｌ2
・２Ｈ2 Ｏ２５ｍｇ、ＭｇＳＯ・７Ｈ2 Ｏ２５ｍｇ及び
ＦｅＣｌ3 ・６Ｈ2 Ｏ５ｍｇと共に蒸留水１リットルに
溶解し、この溶液を水酸化ナトリウムあるいは塩酸を用
い、ｐＨを７．０に調整して分解試験用培地を調製し
た。(Example 1) Meat extract 100 mg, K 2 HPO 4 500 mg, NH 4 were added to the predetermined amount of APE shown in Table 2.
H 2 PO 4 660 mg, NaCl 25 mg, CaCl 2
・ 2H 2 O 25 mg, MgSO.7H 2 O 25 mg and FeCl 3 .6H 2 O 5 mg are dissolved in 1 liter of distilled water, and this solution is adjusted to pH 7.0 with sodium hydroxide or hydrochloric acid and used for a decomposition test medium. Was prepared.
【００１６】前記の分解試験用培地を３００ｍｌ容積の
三角フラスコに１００ｍｌ分注し、１２０℃の温度で２
０分間高圧滅菌して培養液とした。この培養液に一白金
耳の本発明シュウードモナス・エスピーＡＰＥ６株（Ｆ
ＥＲＭ Ｐ−１３８７０）を接種し、２２℃の温度で往
復振盪培養機を用いて振盪培養を２４時間行ったのち、
この培養液１ｍｌを新しい培地に継代し培養した。この
一連の継代培養操作を２回行ったのち、３回目の培地中
における界面活性剤濃度の低減量を測定した。100 ml of the above-mentioned medium for decomposition test was dispensed into an Erlenmeyer flask having a volume of 300 ml, and the medium was kept at 120 ° C. for 2
It was autoclaved for 0 minutes to obtain a culture solution. In this culture medium, one platinum loop of the present invention, Pseudomonas sp. APE6 strain (F
ERM P-13870), and shaking culture was carried out at a temperature of 22 ° C. using a reciprocal shaking culture machine for 24 hours.
1 ml of this culture was subcultured to a new medium and cultured. After performing this series of subculture operations twice, the reduction amount of the surfactant concentration in the medium for the third time was measured.
【００１７】ＡＰＥの分解率と生菌数を測定した結果は
表２に示すとおりであり、本発明の菌株は、ＡＰＥ濃度
２０００ｍｇ／Ｌ以下の濃度範囲においてよく増殖し、
効率よくＡＰＥを分解しうることが判った。The results of measuring the APE decomposition rate and the viable cell count are shown in Table 2. The strain of the present invention proliferates well in the concentration range of APE concentration of 2000 mg / L or less,
It was found that APE can be decomposed efficiently.
【００１８】[0018]
【表２】 [Table 2]
【００１９】（比較例１）比較試験として実施例１にお
ける前記培地にシュウードモナス・エスピーＡＰＥ６株
（ＦＥＲＭ Ｐ−１３８７０）を接種せずに試験を行な
ったところ、ＡＰＥの分解は全く認められなかった。(Comparative Example 1) As a comparative test, a test was conducted without inoculating the above medium in Example 1 with Pseudomonas sp. APE6 strain (FERM P-13870), and no degradation of APE was observed. It was
【００２０】（実施例２及び比較例２）本発明の菌株を
栄養培地で充分に培養して得られた培養液を植物性繊維
からなる粉体に混合し、乾燥して乾燥製剤とした。この
時の乾燥製剤の水分量は、平均５．６％であった。(Example 2 and Comparative Example 2) The culture solution obtained by sufficiently culturing the strain of the present invention in a nutrient medium was mixed with a powder made of plant fiber and dried to give a dry preparation. The water content of the dried preparation at this time was 5.6% on average.
【００２１】実施例１において調製したＡＰＥ２００ｍ
ｇ／Ｌを含有する培養液に、下記に示す三とおりの接種
量の本発明の菌株等を所定量添加し、培養を開始し、新
鮮な培地に２回継代培養を行ったのち、３回目の２４時
間培養において界面活性剤の分解量及び全有機炭素濃度
の除去量を測定した。その結果は表３に示すとおりであ
り、本菌株は乾燥菌剤に加工してもＡＰＥを分解する能
力を失わず、良好な処理を行えることが判明した。なお
ブランク試験としては、標準活性汚泥のみを用いた時の
処理能力を調べた。APE 200m prepared in Example 1
To a culture solution containing g / L, a predetermined amount of the bacterial strain of the present invention or the like having the following three inoculation amounts was added, the culture was started, and the fresh medium was subcultured twice, and then 3 The amount of decomposition of the surfactant and the amount of removal of the total organic carbon concentration were measured in the second 24-hour culture. The results are shown in Table 3, and it was revealed that the strain of the present invention does not lose the ability to decompose APE even when it is processed into a dry fungus agent and can be well treated. In the blank test, the treatment capacity when only standard activated sludge was used was examined.
【００２２】 （試験区） （接種量） Ａ：本菌株 １白金耳植菌 Ｂ：本菌株乾燥菌剤 200mg/l Ｃ：標準活性汚泥＋本菌株乾燥菌剤 汚泥濃度1000mg/l＋乾燥菌剤 200mg/l Ｄ：標準活性汚泥（ブランク試験） 汚泥濃度1000mg/l(Test area) (Inoculation amount) A: This strain 1 platinum loop inoculum B: This strain dry bacteria agent 200 mg / l C: Standard activated sludge + this strain dry bacteria agent Sludge concentration 1000 mg / l + dry bacteria agent 200 mg / l D: Standard activated sludge (blank test) Sludge concentration 1000mg / l
【００２３】[0023]
【表３】 [Table 3]
【００２４】（実施例３）主に家庭廃水を処理している
活性汚泥方式下水処理施設より採取した活性汚泥を用い
てＡＰＥを含有する排水の連続処理試験を行った。容量
１０Ｌの曝気槽を有する活性汚泥連続処理装置を２系列
用意し、この処理系にエキス４０ｇ、ペプトン６０ｇ、
ＮａＣｌ１．５ｇ、ＭｇＳＯ4 ・７Ｈ2 Ｏ１．３ｇ、Ｋ
Ｃｌ０．７ｇ、ＣａＣｌ2 １．８ｇ、ＮａＨＣＯ3 ４２
ｇ及び水道水１リットルからなる合成下水を連続的に流
入させ、３日間活性汚泥を培養した。(Example 3) A continuous treatment test of wastewater containing APE was conducted using activated sludge collected from an activated sludge type sewage treatment facility which mainly treats domestic wastewater. Two series of activated sludge continuous treatment equipment having an aeration tank with a capacity of 10 L were prepared, and 40 g of extract and 60 g of peptone were added to this treatment system.
NaCl1.5g, MgSO 4 · 7H 2 O1.3g , K
Cl 0.7 g, CaCl 2 1.8 g, NaHCO 3 42
g and 1 liter of tap water were continuously introduced, and the activated sludge was cultured for 3 days.
【００２５】なお、活性汚泥連続処理装置の運転は、次
に示すとおりの条件で行った。 運転条件 槽容積 ：１０Ｌ 水滞留時間（ＨＲＴ） ：約８時間 汚泥滞留時間（ＳＲＴ）：約７日 ＴＯＣ−容積負荷 ：０．２Ｋｇ／ｍ3 返送汚泥比 ：４０％ 流入水量 ：２０Ｌ／日 ＭＬＳＳ ：１０００ｍｇ／Ｌ 温度 ：２０±２℃The activated sludge continuous treatment system was operated under the following conditions. Operating conditions Tank volume: 10 L Water retention time (HRT): Approximately 8 hours Sludge retention time (SRT): Approximately 7 days TOC-Volume load: 0.2 Kg / m 3 Return sludge ratio: 40% Influent water volume: 20 L / day MLSS : 1000 mg / L Temperature: 20 ± 2 ° C
【００２６】次に、前記合成下水にＡＰＥ２００ｍｇ／
Ｌを添加した合成下水を用いて活性汚泥の連続処理試験
を行い、培養を継続した。その際に、本菌株添加区に
は、実施例１における分解試験用培地組成で増殖させた
本菌（生菌数１０8 ＣＦＵ／ｍｌ）の培養液を５００ｍ
ｌ投入した。この培養液には１ｍｌ中１０8 個以上の細
胞数が存在していた。また、対照区には添加せず、ブラ
ンク試験とした。Next, 200 mg / APE was added to the synthetic sewage.
A continuous treatment test of activated sludge was performed using synthetic sewage containing L, and the culture was continued. At this time, 500 m of a culture solution of the present bacterium (viable count 10 8 CFU / ml) grown in the medium composition for degradation test in Example 1 was added to the present strain addition section.
1 was added. In this culture medium, there were 10 8 or more cells in 1 ml. A blank test was carried out without adding it to the control group.
【００２７】連続処理試験において経時的に界面活性剤
濃度（ＣＴＡＳ濃度）、排水の有機物量としての全有機
炭素濃度（ＴＯＣ濃度）及び処理水中に浮遊している汚
泥量（ＳＳ濃度）を測定した。その測定結果は、図１な
いし図３に示すとおりであり、処理系に本菌株を投入し
た系と、していない系とでは明らかな差がみられた。In the continuous treatment test, the surfactant concentration (CTAS concentration), the total organic carbon concentration (TOC concentration) as the amount of organic matter in the waste water, and the sludge amount (SS concentration) floating in the treated water were measured over time. . The measurement results are shown in FIGS. 1 to 3, and a clear difference was observed between the system in which this strain was added to the treatment system and the system in which it was not.
【００２８】ＡＰＥが処理系内に流入することにより、
活性汚泥に影響を生じ、ＡＰＥを分解しなくなるだけで
なく、処理水中のＴＯＣ濃度が上昇すると共に、処理水
中に汚泥の流出が起こった。これに対して本菌株を投入
した系においては、ＡＰＥを分解し、良好な処理を行う
ことができた。When APE flows into the processing system,
Not only did the activated sludge be affected and APE was not decomposed, but the TOC concentration in the treated water increased, and sludge flowed out into the treated water. On the other hand, in the system containing this strain, APE was decomposed and good treatment could be performed.
【００２９】[0029]
【発明の効果】本発明のシュウドモナス・エスピーＡＰ
Ｅ６株（ＦＥＲＭ Ｐ−１３８７０）を生活排水、産業
排水あるいは非イオン界面活性剤等により汚染された河
川水などに接触させることにより、それら排水に含まれ
ているＡＰＥを効率良く分解除去することができるの
で、環境浄化に多大な効果を与える。EFFECTS OF THE INVENTION Pseudomonas sp. AP of the present invention
By contacting E6 strain (FERM P-13870) with domestic wastewater, industrial wastewater, or river water contaminated with nonionic surfactants, etc., the APE contained in these wastewater can be efficiently decomposed and removed. As a result, it has a great effect on environmental purification.
【図１】実施例３における本発明の微生物を用いた排水
処理系及び用いなかった排水処理系の処理水中の界面活
性剤濃度の経日変化を示すグラフ図。FIG. 1 is a graph showing the daily change of the surfactant concentration in the treated water of the wastewater treatment system using the microorganism of the present invention and the wastewater treatment system not using the same in Example 3.
【図２】実施例３における本発明の微生物を用いた排水
処理系及び用いなかった排水処理系の処理水中の全有機
炭素濃度の経日変化を示すグラフ図。FIG. 2 is a graph showing the daily change of the total organic carbon concentration in the treated water of the wastewater treatment system using the microorganism of the present invention and the wastewater treatment system not using it in Example 3.
【図３】実施例３における本発明の微生物を用いた排水
処理系及び用いなかった排水処理系の処理水中の浮遊汚
泥（ＳＳ）濃度の経日変化を示すグラフ図。FIG. 3 is a graph showing the change over time in suspended sludge (SS) concentration in the treated water of the wastewater treatment system using the microorganism of the present invention and the wastewater treatment system not using the same in Example 3.
Claims (2)
s sp.)ＡＰＥ６株（工業技術院生命工学工業技術研究所
寄託菌、受託番号ＦＥＲＭ Ｐ−１３８７０）として寄
託されているポリオキシエチレンノニルフェニルエーテ
ルに対して分解性能を示す微生物。1. Pseudomona sp.
s sp.) APE6 strain (deposited bacterium, Institute of Life Science and Technology, Institute of Industrial Science and Technology, deposit number FERM P-13870), which is a microorganism showing a decomposing ability for polyoxyethylene nonylphenyl ether.
s sp.)ＡＰＥ６株（工業技術院生命工学工業技術研究所
寄託菌、受託番号ＦＥＲＭ Ｐ−１３８７０）をポリオ
キシエチレンノニルフェニルエーテル含有排水処理系に
加えて培養し、処理系のポリオキシエチレンノニルフェ
ニルエーテル含有濃度を減少させることを特徴とする微
生物の使用法。2. Pseudomona sp.
s sp.) APE6 strain (deposited bacterium of the Institute of Biotechnology, Institute of Industrial Science, deposit number FERM P-13870) was added to a polyoxyethylene nonylphenyl ether-containing wastewater treatment system and cultured to give polyoxyethylene nonyl of the treatment system. Use of a microorganism characterized by reducing the concentration of phenyl ether.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP33953493A JPH07155173A (en) | 1993-12-02 | 1993-12-02 | Microbe exhibiting degradative prformance for polyoxyethylene nonyl phenyl ether |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP33953493A JPH07155173A (en) | 1993-12-02 | 1993-12-02 | Microbe exhibiting degradative prformance for polyoxyethylene nonyl phenyl ether |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07155173A true JPH07155173A (en) | 1995-06-20 |
Family
ID=18328391
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP33953493A Pending JPH07155173A (en) | 1993-12-02 | 1993-12-02 | Microbe exhibiting degradative prformance for polyoxyethylene nonyl phenyl ether |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07155173A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008207073A (en) * | 2007-02-23 | 2008-09-11 | Idemitsu Kosan Co Ltd | Decomposition method of ethers |
| JP2015033331A (en) * | 2013-08-07 | 2015-02-19 | 積水アクアシステム株式会社 | Microorganism and wastewater treatment method |
-
1993
- 1993-12-02 JP JP33953493A patent/JPH07155173A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008207073A (en) * | 2007-02-23 | 2008-09-11 | Idemitsu Kosan Co Ltd | Decomposition method of ethers |
| JP2015033331A (en) * | 2013-08-07 | 2015-02-19 | 積水アクアシステム株式会社 | Microorganism and wastewater treatment method |
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