JPH0716995Y2 - Membrane detachable measuring electrode - Google Patents

Description

[Detailed Description of the Invention] [Industrial field of application] The present invention relates to a membrane detachable measurement electrode, and more particularly, it is used in the case of measuring the concentration of albumin in urine by an enzyme immunoelectrode method, for example. Like the enzyme immunoelectrode, the immunity (antigen-antibody) is applied to the tip electrode part of the measuring electrode body that is composed of a bipolar polarohydrogen peroxide
Removably attach a predetermined membrane such as reaction treatment membrane,
The present invention relates to an improvement of a membrane detachable measuring electrode configured to make a predetermined measurement.

[Conventional technology]

As is apparent from FIG. 6 showing the state in which the membrane is attached, the conventional membrane detachable measurement electrode of this type has a predetermined membrane on the tip electrode portion y of the measurement electrode body x in order to perform a predetermined measurement. c (for example, an enzyme-immobilized membrane cleaved to an appropriate size, an antibody-immobilized carrier membrane is allowed to act with an antigen as a sample to be measured, or vice versa, and a sandwich method or a competitive method is performed. When attaching an immunoreaction treatment membrane that is labeled with an enzyme by
The film c itself is applied to the end face response part of the tip electrode part y, then the fixing ring member d made of synthetic resin is attached to the outer peripheral surface part of the tip electrode part y, and the periphery of the film c is the tip electrode. The outer peripheral surface of the portion y and the inner peripheral surface of the fixing ring member d are externally fitted so that the membrane c is fixed to the tip electrode portion y. In order to remove the membrane c from the tip electrode part y of the measurement electrode body x in order to replace it with a new membrane for the next measurement, first, the outer peripheral surface of the tip electrode part y is fixed. The membrane ring c is removed, and then the membrane c itself is peeled off from the response end surface portion of the tip electrode portion y, so that the membrane c can be attached to and detached from the tip electrode portion y of the measurement electrode body x. Was there.

[Problems to be solved by the invention]

However, in the above-mentioned conventional membrane detachable measurement electrode, since the membrane c itself has to be handled by itself, various treatments against it (for example, antibody or antigen immobilization treatment, blocking treatment, immune reaction treatment,
It is very difficult to handle it when performing a buffer separation process, etc., and since the membranes c after the treatment cannot be overlapped with each other, it needs to be stored with considerable caution and a large space is required. Furthermore, the attachment / detachment of the membrane c to / from the tip electrode portion y of the measurement electrode body x must be performed by the complicated manual operation as described above, and therefore the membrane c and the response portion of the tip electrode portion y are easily soiled. Not only is there an inconvenience, but especially in the case of an enzyme immunoelectrode, etc., in which it is necessary to replace the membrane c for each sample, the attachment / detachment operation is extremely troublesome. The film c is fixed to the tip electrode part y by fitting the fixing ring member d onto the outer peripheral surface of the tip electrode part y with the film c itself being applied to the end face response part of y. Because It is almost impossible to always set the tension of c to a constant value. Therefore, especially when re-measuring the same film c, the mounting condition of the film c and the reproducibility of the measurement result are very poor. There were various problems such as being lost.

It is an object of the present invention to develop and provide a membrane detachable measurement electrode having a configuration capable of solving all of the conventional problems at once.

[Means for solving problems]

In order to achieve the above object, the membrane detachable measurement electrode according to the present invention has a measurement electrode body having an electrode portion provided at the tip of a support and a shape and a size that can be inserted into and removed from the tip electrode portion. In a membrane detachable measuring electrode comprising a membrane chip in which a predetermined membrane is integrally held in advance on one end surface side of a cylindrical body having, a male screw portion is provided on the outer surface of the support, and this male screw A nut member for positioning and a holder member with a female screw are screwed in this order to the portion, and an engaging portion is provided on the holder member side and an engaged portion is provided on the membrane chip side. The feature is that the membrane chip is detachably attached to the holder member by the stopper.

[Action]

The action exerted by such a characteristic configuration is as follows.

That is, in the membrane detachable measurement electrode according to the present invention,
As will be more apparent from the description of Examples below, a male screw portion is provided on the outer surface of the support, and a positioning nut member and a female screw holder member are screwed to this male screw portion in this order, and the holder member Side is provided with a locking portion, and the membrane chip side is provided with a locked portion.
Since the membrane chip is detachably attached to the holder member by the locked portion, when handling the membrane,
The membrane itself can be handled very easily without directly touching it, and the membrane chips after processing can be stored easily by stacking them in a small space because the cylindrical body functions as a spacer. Without having to perform the complicated and cumbersome operations of handling the membrane itself and the fixing ring separately, the entire membrane chip has a tubular body portion so that the membrane itself is not directly touched and the tip is swept. It can be done with a one-touch operation that fits in and out from the electrode part (fitting attachment and detachment), and it is extremely easy and reliable to perform the attachment and detachment operation without the possibility of contaminating the membrane or the response part of the tip electrode part. Of course, it is possible to provide a locking portion and a locked portion on the membrane chip and the holder member and set the mounting position of the membrane chip, so that the measurement electrode main body of the membrane chip can be operated by one-touch operation. Can always be fixed mounting position relative to the tip electrode portion (fitting depth) in,
Since it is easy to realize that the tension of the film is always constant, the accuracy and reliability of the measurement can be greatly improved, and good reproducibility can be secured even when re-measurement is performed on the same film. It became so.

〔Example〕

Hereinafter, various specific embodiments of the present invention will be described with reference to the drawings.

FIG. 1 to FIG. 4 show an embodiment of an enzyme immunoelectrode as an example of a membrane-detachable measurement electrode and a modification of its essential part.

That is, this enzyme immunoelectrode is used, for example, in the case of measuring the concentration of urinary albumin by the enzyme immunoelectrode method, and the measuring electrode body X as shown in FIG. 1 and the measuring electrode body thereof. It is a member that is inserted into and removed from (inserted into and removed from) and is composed of a membrane chip Z as shown in FIG.

As shown in the partial sectional side view of FIG. 1 (a) and the bottom view of FIG. 1 (b), the measuring electrode body X includes an outer cylinder 1 and an inner cylinder 2 made of synthetic resin having electric insulation. A platinum electrode (anode) 4 in the center is attached to the tip of a support 3 composed of
And a peripheral silver electrode (cathode) 5 and a temperature compensating electrode 6 molded with an epoxy resin 6'having a high thermal conductivity, and its (lower) end face projects outward toward the center. The curved electrode portion Y is disposed, and a holder member 7 made of synthetic resin for the membrane chip Z, which will be described in detail later, and the positioning for the holder member 7 are provided in the outer lower half portion of the outer cylinder 1. The nut member 8 is screwed to form a so-called bipolar polaro type hydrogen peroxide electrode.

As shown in the figure, the holder member 7 includes a plurality of holder members 7 extending downward from the annular screwing portion 7A (in this example, 2
Book-like elastic clamping rod-shaped bodies 7B ... And, at the tips of the rod-shaped bodies 7B ..., locking projections 7b ... (an example of locking portions) for the membrane chip Z are formed respectively. .

Further, the holder member 7 and the positioning nut member 8 have the membrane chip Z with respect to the tip electrode portion Y of the measurement electrode main body X, as will be apparent from the later description.
The position regulating mechanism P is configured to uniquely and arbitrarily determine the fitting depth of.

On the other hand, as shown in the vertical side view of FIG. 2 (a) and the plan view of FIG. 2 (b), the membrane chip Z is fitted into (removed from) the tip electrode portion Y of the measurement electrode body X. It has a shape and a size capable of being mounted and removed (that is, has an inner diameter substantially equal to the outer diameter of the tip electrode portion Y, and its inner peripheral surface closely fits to the outer peripheral surface of the tip electrode portion Y). On one end surface side (lower side in this example) of a relatively low-height (ring-shaped) cylindrical body E made of synthetic resin (for example, vinyl chloride, ABS, polycarbonate, polypropylene, etc.) formed in An antibody or a polymer film or a porous film thereof, which has been previously subjected to a hydrophilic or hydrophilic treatment such as a predetermined membrane C (eg, cellulose acetate, polycarbonate, polypropylene, silicon, Teflon (registered trademark)), is used. A carrier film made by fixing the original, by the action of antigen or antibody as the measurement target sample, antigen -
(An immunoreaction treatment membrane that causes an antibody reaction and is labeled with an enzyme by a sandwich method or a competition method)
Are directly held by means such as adhesion, heat fusion or ultrasonic fusion, and are thereby integrally held. And, in the outer peripheral surface portion of the tubular body E,
An engaging groove 9 (an example of a locked portion) corresponding to the tip locking projections 7b of the elastic pinching rods 7B of the holder member 7 of the position regulating mechanism P provided on the measurement electrode body X side.
Further, an inner guide taper surface 10 for fitting the tip electrode portion Y of the measurement electrode body X into the tubular body E is formed on the inner peripheral portion of the upper end of the tubular body E. At the same time, on the outer peripheral portion of the upper end of the cylindrical body E, the projection 7b for locking the tip of the elastic pinching rod 7B ...
An outer guide taper surface 11 for engaging the engaging groove 9 with the engaging groove 9 is formed. In FIG. 2 (B), S indicates an immune (antigen-antibody) reaction-treated portion on the membrane C.

Thus, the membrane chip Z in which the tubular body E and the membrane C are integrated
By configuring the above, the membrane C can be handled as the entire membrane chip Z without the need to handle the membrane C itself alone as in the conventional case, and therefore various treatments (eg, antibody or antigen treatment) on the membrane C can be performed. When performing a fixing process, a blocking process, an immune reaction process, a buffer separation process, etc.), by holding the tubular body E of the membrane chip Z, the membrane C itself is not directly touched and it is very easy. You can do that. Further, after the treatment, the membrane chips Z can be stacked because the tubular body E that is a peripheral constituent member thereof can function as a spacer, and thus a plurality of the membrane chips Z can be stacked if they are in the same vertical posture. Can be done easily and in a small space.

When the membrane chip Z is attached to or detached from the tip electrode portion Y of the measurement electrode body X, the position regulating mechanism P (the holder member 7 and the positioning nut member 8) is appropriately adjusted and set. By holding the tubular body E, which is a peripheral constituent member of the membrane chip Z, by hand, the entire membrane chip Z is fitted into and removed from the tip electrode portion Y and the holder member 7 (fitting attachment and detachment).
Both can be performed manually, but the following operation is more convenient.

That is, regarding the mounting operation, as shown by the solid white outline arrow in FIG. 3, the tip electrode portion Y of the measurement electrode body X is
By pressing and inserting from above into the tubular body E of the membrane chip Z set in the processing tray 12,
As indicated by the dotted line in the figure, the membrane chip Z may be automatically fitted at a predetermined position (fitting depth) in the tip electrode portion Y of the measurement electrode body X. That is, at the time of this mounting, as shown by the solid line reciprocating arrow in the figure, in the initial stage of insertion of the tip electrode portion Y into the cylindrical body E, the elastic pinching rods 7B ... Locking protrusion
By the cam action of the outer guide taper surface 11 of the cylindrical body E on the cylindrical body E, the elastic pinching rods 7B are automatically expanded outward, and at the subsequent final stage of insertion, the cylindrical body With the engagement of the locking projections 7b ... With the engaging groove 9 of the body E, the elastic clamping rod-shaped bodies 7B .. It is clamped and fixed (held) at a predetermined position (fitting depth) in the tip electrode portion Y.

Since the lower end surface of the tip electrode portion Y of the measurement electrode body X is formed into a curved surface projecting outward toward the center portion thereof as described above, when the membrane chip Z is attached, the platinum electrode It is possible to effectively prevent bubbles from entering between the film C and the end surface (electrode surface) of the (anode) 4 and the silver electrode (cathode) 5.

In addition, regarding the removal operation, with the tip electrode portion Y of the measurement electrode body X facing the empty processing tray 12, for example, using tweezers or the like, the elastic holding rod-shaped body 7B ... If the intermediate portion is expanded outward as indicated by the dotted arrow in the figure, the film chip Z
Is removed from the tip electrode portion Y of the measurement electrode body X and dropped into the processing tray 12.

FIGS. 4 (a) and 4 (b) show a modified example of the membrane chip Z, which is not directly fixed to the tubular body E of the membrane C like the one described above, but of the tubular body E. One end side (lower side)
In addition, a supporting net body (mesh) 13 made of an elastic material (for example, nylon, tetron, acetate, etc.) that is elastic and hard to cause plastic elongation is attached by means of adhesion, heat fusion or ultrasonic fusion. By indirectly fixing the film C on the net body 13 by using the cylindrical body E
The film C is integrally held on the. In this case, the membrane tip Z is attached to the tip electrode portion Y of the measurement electrode body X.
In the state of being mounted on, the membrane C causes the end face (electrode face) of the platinum electrode (anode) 4 and the silver electrode (cathode) 5 due to the large tension generated with the extension of the supporting net body 13.
Since it is strongly pressed against, the degree of adhesion between the film C and the electrode surface can be ensured very well, and since the film C itself is not stretched excessively, when re-measurement is performed on the same film, It is possible to secure even better reproducibility. A film body may be used instead of the supporting net body (mesh) 13. Other configurations are the same as those described above.

FIG. 5 shows a modified example of the main part (membrane chip Z) in the above-mentioned embodiment, and like the membrane chip Z shown in FIG. 2 used in the above-mentioned embodiment, one end face side (lower side) of the tubular body E. ) Is adhered directly to the membrane C by means such as adhesion, heat fusion or ultrasonic fusion, and a plurality of buffer solutions such as a substrate solution are circulated around the cylindrical body E. Hole 21 ...
Is drilled.

In each of the above-described embodiments, the enzyme immunoelectrode is described as an example of the membrane detachable measurement electrode, but the present invention is not limited to this enzyme immunoelectrode, and for example, a measurement using an enzyme-immobilized membrane is performed. Widely applicable to so-called biosensors such as electrodes and measuring electrodes that use interference blocking membranes for direct measurement of biological components, as well as solid membrane ion electrodes, or membrane detachable measuring electrodes such as diaphragm ion electrodes. It is possible.

[Effect of device]

As is clear from the above detailed description, according to the membrane detachable measurement electrode according to the present invention, one end surface of the cylindrical body having a shape and size that can be inserted into and removed from the tip electrode portion of the measurement electrode body. By preliminarily forming a membrane chip integrally holding a predetermined membrane on the side, when handling the membrane, the membrane itself has a tubular body portion that is a peripheral constituent member of the membrane chip. It can be handled very easily without touching it directly, and even after storage of the membrane chip, it can be done easily and in a small space by stacking tubular bodies as spacers, and the membrane can be attached to and detached from the electrode part. With the simple operation of fitting and unmounting the entire chip (fitting, mounting and withdrawing) to the tip electrode part at once, there is no risk of contaminating the membrane or the response part of the tip electrode part, and it is extremely easy and reliable. Of course, it is possible to provide a male screw portion on the outer surface of the support, and a positioning nut member and a female screw holder member are screwed to the male screw portion in this order, and the holder member and the membrane chip are Since the locking part and the locked part are respectively provided so that the locking position of the membrane chip can be set, the mounting position (fitting position) of the membrane chip to the tip electrode part in the measurement electrode body can be set by an extremely simple one-touch operation. Since it is possible to keep the depth constant at all times and to easily set the membrane tension to always be constant, the accuracy and reliability of the measurement can be greatly improved, and even if the same membrane is re-measured. Even in the case of carrying out, etc., various excellent effects that very good reproducibility can be ensured have been exhibited.

[Brief description of drawings]

1 to 5 show various specific embodiments of the membrane detachable measuring electrode according to the present invention, and FIGS. 1 (a) and 1 (b) are partial cross-sections of the measuring electrode main body in the embodiment of the present invention. A side view and a bottom view, FIGS. 2 (a) and 2 (b) are vertical side views and bottom views of the membrane chip in the embodiment, and FIG. 3 is a partial cross-sectional side view for explaining the operation and action. , 4th
Figures (a) and (b) are vertical side views and bottom views of a modified example of the main part (membrane chip), and FIG. 5 is a vertical side view of a modified example of the main part (membrane chip) in the embodiment of the present invention. Is. Further, FIG. 6 is for explaining the technical background of the present invention and the problems of the prior art, and is a partial cross-sectional side view of a conventional membrane detachable measurement electrode. E ... Cylinder, C ... Membrane, P ... Position control mechanism, X ...
Measuring electrode body, Y ... Tip electrode part, Z ... Membrane chip, 13
...... A net or film made of elastic material.

Front page continuation (72) Kazuo Horiguchi, 14 Kami Toba Mamachi Shi-machi, Minami-ku, Kyoto-shi, Kyoto Prefecture Susumu Kogyo Co., Ltd. (72) Inventor Setsuko Nakanishi 14 Kamitoba Ma-machi Shi, Minami-ku, Kyoto Address Susumu Kogyo Co., Ltd. (72) Inventor Shigeki Guo 14 Kamikaba Mamachishi-cho, Minami-ku, Kyoto City, Kyoto Prefecture Susumu Kogyo Co., Ltd. (72) Shoji Nishimura 2-5-1 Mishima, Settsu-shi, Osaka Shionogi Pharmaceutical Co., Ltd. (56) Bibliography S. 58-12130 (JP, Y1) S. 51-153644 (S. 53-70289) The contents of the specification and drawings attached to the application (JP, U) A microfilm (JP, U) of the specifications and drawings attached to the application for Japanese Patent Application No. 57-164381 (Japanese Utility Model Application No. 59-68253)

Claims (4)

[Scope of utility model registration request] 1. A measurement electrode main body having an electrode portion provided at the tip of a support, and a predetermined film on one end face side of a cylindrical body having a shape and size that can be inserted into and removed from the tip electrode portion. In the membrane detachable measurement electrode, which is configured by a membrane chip formed by integrally holding the above, a male screw portion is provided on the outer surface of the support, and a positioning nut member and a female screw holder member are provided on the male screw portion. By screwing in order, a locking portion is provided on the holder member side and a locked portion is provided on the membrane chip side, respectively, and the membrane chip can be detachably attached to the holder member by these locking portions and locked portions. Membrane detachable measurement electrode characterized in that it is configured. 2. The membrane according to claim [1], wherein the membrane chip is constructed such that the predetermined membrane itself is directly fixed to one end surface side of the tubular body. Detachable measuring electrode. 3. In forming the membrane chip, a supporting net body or film body made of an elastic material that is elastic is fixed to one end surface side of the tubular body, and the supporting net body or film body is mounted on the supporting net body or film body. The membrane detachable measurement electrode according to claim 1, wherein the predetermined membrane is mounted on the utility model. 4. A utility model registration claim in which the end face of the tip electrode portion of the measuring electrode body is formed into a curved surface projecting outward toward the center thereof.
A membrane-detachable measurement electrode according to any one of items.