JPH072961U - Automatic scattered light measuring device - Google Patents

Abstract

(57) [Summary] (Modified) [Purpose] To provide an automatic scattered light measuring device for detecting transmitted light, scattered light deviated from the optical axis, fluorescence, etc. separately from transmitted light. [Structure] Mirrors 4 and 4 '... are arranged between measuring cells 1 and 1'which are arranged at equal intervals on a rotating turret, and a compact automatic system capable of accurately detecting transmitted light 6, scattered light 8 and fluorescence. It is a scattered light measuring device.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention is an automatic scattering light measuring device that uses a turret plate or the like in a discrete method of a biochemical analyzer, an immunoanalyzer, or a clinical chemistry automatic analyzer, which is deviated from the optical axes of scattered light and fluorescence. It relates to an automatic scattered light analyzer that measures the emitted light.

[0002]

[Prior art]

 The following methods are known as methods that do not incorporate transmitted light in the measurement of scattered light, fluorescence, etc. The first method has been used since ancient times. First, an optical system called dark-field illumination is used. Direct light is blocked by a disc that separates the sample, and the light that passes through the periphery is collected to collect light. After receiving light at, measure. Second, the detector is installed diagonally at an angle to the transmitted light. In general, this angle is often 90 °, and specifically, it is detected from the side surface or the upper surface. For example, the device disclosed by the applicant as an optical measuring device in JP-A-63-208732 and JP-A-63-208733 is applicable to this. In addition, a condenser lens for condensing scattered light disclosed in Japanese Utility Model Publication No. 61-20522 is arranged at a focal point of transmitted light, and a pinhole plate for transmitting light and a pinhole plate for scattered light are respectively provided. There is one that has a detector of 2 and obtains transmitted light and scattered light from both measuring instruments.

[0003]

[Problems to be solved by the device]

 In the first method described above, the optical system becomes complicated and a detector dedicated to scattered light is provided. Among the second methods, the upper surface can be detected, but the measurement error is large if the flatness of the liquid surface is not guaranteed, such as the occurrence of bubbles. Although side-view detection is a standard method for static measurement, it is not possible with automatic measuring equipment because the measuring cell is moving. Although it is theoretically possible to detect from an oblique direction, stray light increases because it picks up reflected light from the surface of the measuring cell, which is not a good measurement method. In the third method, it is often difficult to separate transmitted light and scattered light. For the above reasons, the present invention proposes a device that applies a light beam projected from a fixed light source to a moving measuring cell and measures scattered light simply and stably with a fixed detector.

[0004]

[Means for Solving the Problems]

 In the present invention, scattered light from the side of the measuring cell is reflected once in the direction of transmitted light using a mirror and guided to the detector. The direction of the mirror does not necessarily have to be parallel to the scattered light and the transmitted light, and may be any direction that is not affected by the adjacent measuring cell.

[0005]

【Example】

 An embodiment of the present invention will be described with reference to FIG. In FIG. 1, there are measuring cells 1 arranged in parallel at equal intervals on a turret 3 which rotates about a rotation center 3 ', and mirrors 4, 4' ... Are arranged between adjacent measuring cells 1 '. It is set up. The light flux emitted from the light source 2 is incident on the measurement cell 1, part of which is transmitted by the fine particles of the suspension in the measurement cell 1, and part of which is scattered light. The transmitted light 6 is measured by a detector 7 according to a conventional method, and at the same time, the scattered light 8 is reflected by a mirror 4 and guided to a detector 9 for measurement.

[0006]

[Effect of device]

 For the biochemical automatic analyzer, immunological analyzer, and clinical chemistry automatic analyzer according to the present invention, the best method is selected according to the item to be measured. This device does not use an optical system dedicated to light fluxes such as scattered light and fluorescence that deviate from the optical axis of the light source, and separates and receives light using a mirror from a cell for measuring transmitted light, and allows simultaneous and simultaneous measurement of transmitted light. You can do it. Moreover, it provides a compact device that is easy to operate.

[Brief description of drawings]

 It is explanatory drawing of the automatic scattered light measuring apparatus which concerns on this invention.

[Explanation of symbols]

 1 Measuring cell 1'Measuring cell 2 Light source 3 Turret 3'Rotation center 4 Mirror 4'Mirror 5 Light flux from light source 6 Transmitted light flux 7 Transmission detector 8 Scattered light 9 Scattered light detector 10 Opening hole of rotating turret

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Yoshio Kawai, 4-8-5 Nakanokami-cho, Hachioji-shi, Tokyo Inside Nippon Tektron Corp.

Claims (1)

[Scope of utility model registration request] 1. In a biochemical or immunoassay device, mirrors are arranged between measuring cells arranged at equal intervals on a rotating turret to detect scattered light, fluorescence, etc., and automatically scattered light. measuring device.