JPH0729840Y2 - Enzyme reactor - Google Patents
Enzyme reactorInfo
- Publication number
- JPH0729840Y2 JPH0729840Y2 JP1988049331U JP4933188U JPH0729840Y2 JP H0729840 Y2 JPH0729840 Y2 JP H0729840Y2 JP 1988049331 U JP1988049331 U JP 1988049331U JP 4933188 U JP4933188 U JP 4933188U JP H0729840 Y2 JPH0729840 Y2 JP H0729840Y2
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- immobilized enzyme
- reactor
- solution
- immobilized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000790 Enzymes Proteins 0.000 title claims description 14
- 102000004190 Enzymes Human genes 0.000 title claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- 239000002994 raw material Substances 0.000 claims description 13
- 239000007853 buffer solution Substances 0.000 claims description 12
- 238000005406 washing Methods 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 108010093096 Immobilized Enzymes Proteins 0.000 description 31
- 229920000642 polymer Polymers 0.000 description 11
- 229940088598 enzyme Drugs 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 239000000047 product Substances 0.000 description 7
- 238000003756 stirring Methods 0.000 description 5
- 238000011001 backwashing Methods 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 229920001661 Chitosan Polymers 0.000 description 2
- 108010068370 Glutens Proteins 0.000 description 2
- 102000057297 Pepsin A Human genes 0.000 description 2
- 108090000284 Pepsin A Proteins 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 235000021312 gluten Nutrition 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229940111202 pepsin Drugs 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【考案の詳細な説明】 〔産業上の利用分野〕 本考案は、酵素を種々の担体に固定化した固定化酵素を
用いる反応器に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial application] The present invention relates to a reactor using an immobilized enzyme in which the enzyme is immobilized on various carriers.
固定化酵素を用いる従来の反応器の一例を第２図に示
す。第２図において、１は原料であるタンパク質溶液な
どの高分子物質溶液の入口ライン、２は固定化酵素を充
填してなる反応器、３は製品の出口ラインである。この
ような反応器を用いると、原料溶液中の高分子物質が反
応器２中の固定酵素充填層部に集積して充填層部での閉
塞が発生し、原料溶液を送液する時に圧力損失が著しく
上昇して運転上の不具合が生ずるほか、高分子物質の固
定化酵素への付着などが生じ固定化酵素の活性の低下を
来たすという不具合を生じさせる。このため、固定化酵
素を利用する高分子物質用酵素反応器は事実上実用化さ
れていないのが現状である。An example of a conventional reactor using an immobilized enzyme is shown in FIG. In FIG. 2, 1 is an inlet line for a polymer solution such as a protein solution as a raw material, 2 is a reactor filled with immobilized enzyme, and 3 is an outlet line for a product. When such a reactor is used, the polymer substance in the raw material solution accumulates in the fixed enzyme packed bed portion in the reactor 2 to cause blockage in the packed bed portion, resulting in a pressure loss when feeding the raw material solution. Causes a problem in that the activity of the immobilized enzyme decreases due to the adhesion of the polymer substance to the immobilized enzyme and the like. For this reason, the present situation is that an enzyme reactor for polymer substances using an immobilized enzyme has not been practically used in practice.
本考案は上記技術水準に鑑み、固定化酵素を用いて合目
的に酵素反応を生起させることができ、かつ上述したよ
うな不具合のない酵素反応器を提供しようとするもので
ある。In view of the above-mentioned state of the art, the present invention intends to provide an enzyme reactor which can purposely cause an enzymatic reaction by using an immobilized enzyme and which does not have the above-mentioned problems.
本考案は、固定化酵素充填層を設け原料溶液を送液して
反応させる固定層式酵素反応器であって、固定化酵素充
填層中に攪拌機を付設し、緩衝液による洗浄時に固定化
酵素充填層を攪拌するようにしたことを特徴とする酵素
反応器である。The present invention is a fixed-bed enzyme reactor in which an immobilized enzyme packed bed is provided to feed and react a raw material solution, and a stirrer is provided in the immobilized enzyme packed bed to fix the immobilized enzyme during washing with a buffer solution. An enzyme reactor characterized in that a packed bed is agitated.
以下、本考案の一実施例を第１図によつて説明する。第
１図において４は攪拌機で、その他第２図と同一部分に
は同一符号を付している。An embodiment of the present invention will be described below with reference to FIG. In FIG. 1, reference numeral 4 is a stirrer, and the same parts as those in FIG. 2 are denoted by the same reference numerals.
攪拌機４は固定化酵素充填層の下部に設けられており、
これを回転させることにより固定化酵素充填層全体を攪
拌することができる。The stirrer 4 is provided below the immobilized enzyme packed bed,
By rotating this, the whole immobilized enzyme-packed bed can be stirred.
原料であるタンパク質溶液などの高分子物質溶液を入口
ライン１を経て固定化酵素を充填している反応器２中に
導き、こゝで酵素反応を行わせ、製品出口ライン３から
反応製品を取出す。この際、攪拌機４は停止しておく。A polymeric material solution such as a protein solution, which is a raw material, is introduced into a reactor 2 filled with an immobilized enzyme through an inlet line 1, an enzymatic reaction is performed there, and a reaction product is taken out from a product outlet line 3. . At this time, the stirrer 4 is stopped.
反応中は固定化酵素は攪拌されていないので反応時間の
経過と共に、原料高分子物質が固定化酵素の充填部のす
き間に蓄積したり、固定化酵素に付着して、圧損の増
大、反応の不活性化が進んでくる。その際、入口ライン
１からの原料高分子物質の供給を止め、攪拌機４を駆動
させて固定化酵素の充填層を攪拌すると同時に、入口ラ
イン１又は製品出口ライン３を利用して緩衝液を通液す
るようにする。緩衝液の通液方向は入口ライン１→製品
出口ライン３でも、その逆でもよい。Since the immobilized enzyme is not stirred during the reaction, as the reaction time elapses, the raw material polymer substance accumulates in the gap of the packed portion of the immobilized enzyme or adheres to the immobilized enzyme to increase the pressure loss and the reaction. Inactivation is progressing. At that time, the supply of the raw material polymer substance from the inlet line 1 is stopped, the stirrer 4 is driven to stir the packed bed of the immobilized enzyme, and at the same time, the buffer solution is passed through the inlet line 1 or the product outlet line 3. Try to liquidate. The flow direction of the buffer solution may be the inlet line 1 → the product outlet line 3 or vice versa.
攪拌機４による攪拌と緩衝液の通液により、固定化酵素
の充填部のすき間に蓄積した高分子物質及び固定化酵素
に付着した高分子物質は効果的に洗浄除去される。そこ
で再び入口ライン１より原料である高分子物質溶液を導
入し、反応を継続する。By stirring with the stirrer 4 and passing the buffer solution, the polymer substance accumulated in the gap of the packed portion of the immobilized enzyme and the polymer substance attached to the immobilized enzyme are effectively washed and removed. Then, the polymer material solution as a raw material is introduced again from the inlet line 1 to continue the reaction.
攪拌機で固定化酵素充填層部を攪拌しながら緩衝液を通
液しうるようにしてあるので、充填層部のすき間に蓄積
したり固定化酵素に付着した高分子物質を効果的に除去
することができる。Since the buffer solution can be passed through the stirrer while stirring the packed bed of immobilized enzyme, it is necessary to effectively remove the polymer substances accumulated in the gap of the packed bed and attached to the immobilized enzyme. You can
小麦グルテン溶液を原料として、真比重約1.6の粒径約
0.7mmのキトサンビーズ担体（粒径0.1〜3mmの各種のビ
ーズが市販されている）に固定化したペプシン（加水分
解酵素）による部分加水分解試験を、第１図の本考案反
応器と第２図の従来の反応器を用いて行つた。試験条件
は固定化酵素2l充填カラムに小麦グルテン溶液を2l/h
〔即ちsv＝1h-1〕で通液した。固定化酵素はキトサンビ
ーズ1g当りペプシンを5.5mg（初期）固定化したものを
使用した。この結果を表１に示す。Using wheat gluten solution as a raw material, the true specific gravity is about 1.6 and the particle size is about
A partial hydrolysis test using pepsin (hydrolase) immobilized on 0.7 mm chitosan bead carrier (various types of beads having a particle size of 0.1 to 3 mm is commercially available) was carried out using the reactor of the present invention shown in FIG. This was done using the conventional reactor shown. The test conditions were 2 l / h of wheat gluten solution in a column packed with 2 l of immobilized enzyme.
[That is, sv = 1 h −1 ] was passed. As the immobilized enzyme, 5.5 mg (initial) of pepsin was immobilized per 1 g of chitosan beads. The results are shown in Table 1.
No.1は第２図の従来の反応器を用い、何んら洗浄せずに
運転した場合、No.2は第２図の従来の反応器を用い、１
日１回緩衝液（比重約１、pH3酢酸緩衝液）を逆洗しな
がら運転した場合、No.3は第１図の本考案反応器を用
い、１日１回充填層内をプロペラ型攪拌機で攪拌しなが
ら上記と同じ緩衝液で逆洗して運転した場合である。No. 1 uses the conventional reactor shown in FIG. 2, and when operated without any cleaning, No. 2 uses the conventional reactor shown in FIG.
When operated while backwashing the buffer solution (specific gravity: about 1, pH3 acetate buffer solution) once a day, No. 3 uses the reactor of the present invention shown in Fig. 1 and once a day the propeller type agitator in the packed bed. This is the case where the device is operated by backwashing with the same buffer solution as above while stirring.
「“相対活性”とは運転前の固定酵素の活性度を100％
としたときの相対的な活性度を示す。」 上記表１の結果から、本考案の攪拌機を付設した反応器
を用い、時々（この事例では１日１回）洗浄しながら運
転することによつて、固定化酵素充填層での圧力損失の
増加を抑え、しかも固定化酵素の相対活性低下も抑制す
ることができることが明らかである。なお、この洗浄効
果は攪拌機なしで緩衝液の逆洗を行つた場合（No.2）に
比べても明らかに効果が大きいことが分る。 "" Relative activity "means the activity of fixed enzyme before operation is 100%
The relative activity is shown. From the results in Table 1 above, by using the reactor equipped with the stirrer of the present invention with occasional washing (once a day in this case) while washing, the pressure loss in the immobilized enzyme packed bed was reduced. It is clear that the increase can be suppressed, and further the decrease in the relative activity of the immobilized enzyme can be suppressed. In addition, it can be seen that this cleaning effect is obviously greater than that in the case where backwashing of the buffer solution is performed without a stirrer (No. 2).
なお、固定化酵素の作成法は、酵素溶液中に固定化担体
を浸漬して、１〜２時間ゆつくり攪拌しながら放置し、
その後緩衝液で洗浄し、架橋剤に浸漬し、再び緩衝液で
洗浄することによつて行われるので、攪拌機を付設した
本考案反応器では担体への酵素の固定化も行うことがで
きるという付加的効果を奏する。The immobilized enzyme was prepared by immersing the immobilized carrier in the enzyme solution, allowing it to stand for 1-2 hours while stirring,
After that, it is carried out by washing with a buffer solution, immersing in a cross-linking agent, and then washing with a buffer solution again. Therefore, in the reactor of the present invention equipped with a stirrer, it is possible to immobilize the enzyme on the carrier. Produce the desired effect.
以上、本考案反応器につき、原料高分子物質溶液を反応
器２の上方より供給し、下方より製品を取出す方式につ
いて説明したが、下方より原料高分子物質溶液を反応器
に供給し上方より製品を取出すようにしてもよいことは
勿論である。また緩衝液による洗浄も上向流、下向流の
いずれの方式を採用してもよいが、好ましくは上向流で
あり、固定化酵素充填層部に蓄積する高分子物質と固定
化酵素の比重の関係によつて選択すべきである。As described above, in the reactor of the present invention, the method of supplying the raw material polymer solution from the upper side of the reactor 2 and taking out the product from the lower side has been described. The raw material polymer solution is supplied to the reactor from the lower side and the product is fed from the upper side. Of course, you may take out. Further, washing with a buffer solution may employ either an upflow or downflow method, but an upflow is preferable, and a high-molecular substance accumulated in the immobilized enzyme packed bed and the immobilized enzyme are preferably used. It should be selected according to the relationship of specific gravity.
本考案により、固定化酵素充填層での圧力損失の増加を
抑え、かつ固定化酵素の相対活性低下を抑制しうる酵素
反応器が提供される。INDUSTRIAL APPLICABILITY The present invention provides an enzyme reactor capable of suppressing an increase in pressure loss in an immobilized enzyme packed bed and suppressing a decrease in relative activity of an immobilized enzyme.
第１図は本考案の、第２図は従来の固定化酵素を使用し
た反応器の概要の説明図である。 図において、１は原料入口ライン、２は固定化酵素を充
填した反応器、３は製品出口ラインである。FIG. 1 is a schematic diagram of the present invention, and FIG. 2 is a schematic diagram of a conventional reactor using an immobilized enzyme. In the figure, 1 is a raw material inlet line, 2 is a reactor filled with immobilized enzyme, and 3 is a product outlet line.
───────────────────────────────────────────────────── フロントページの続き (72)考案者 松本 和典 神奈川県横浜市神奈川区高島台24番地１号 (56)参考文献 特開 昭62−183848（ＪＰ，Ａ) 特開 昭55−37130（ＪＰ，Ａ) 特開 昭62−294076（ＪＰ，Ａ) ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Creator Kazunori Matsumoto 24-1 Takashimadai, Kanagawa-ku, Yokohama, Kanagawa Prefecture (56) References JP-A-62-183848 (JP, A) JP-A-55-37130 (JP) , A) JP-A-62-194076 (JP, A)
Claims (1)
て反応させる固定層式酵素反応器であって、固定化酵素
充填層中に攪拌機を付設し、緩衝液による洗浄時に固定
化酵素充填層を攪拌するようにしたことを特徴とする酵
素反応器。1. A fixed-bed enzyme reactor in which an immobilized enzyme-packed layer is provided to feed a raw material solution for reaction, and a stirrer is provided in the immobilized enzyme-packed layer to immobilize the solution when washing with a buffer solution. An enzyme reactor characterized in that an enzyme packed bed is agitated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1988049331U JPH0729840Y2 (en) | 1988-04-14 | 1988-04-14 | Enzyme reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1988049331U JPH0729840Y2 (en) | 1988-04-14 | 1988-04-14 | Enzyme reactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01155398U JPH01155398U (en) | 1989-10-25 |
| JPH0729840Y2 true JPH0729840Y2 (en) | 1995-07-12 |
Family
ID=31275440
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1988049331U Expired - Lifetime JPH0729840Y2 (en) | 1988-04-14 | 1988-04-14 | Enzyme reactor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0729840Y2 (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5537130A (en) * | 1978-09-06 | 1980-03-15 | Snow Brand Milk Prod Co Ltd | Active reactor comprising rotating column with immobilized enzyme |
| JPS62183848A (en) * | 1986-02-07 | 1987-08-12 | Shokuhin Sangyo Baioriakutaa Syst Gijutsu Kenkyu Kumiai | Stirring type packed bed reactor |
| DE3618465A1 (en) * | 1986-06-02 | 1987-12-03 | Hoechst Ag | CARRYING OUT BIO CATALYST REACTIONS IN A FLUID BED REACTOR WITH A 2-PHASE LIQUID SYSTEM |
-
1988
- 1988-04-14 JP JP1988049331U patent/JPH0729840Y2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01155398U (en) | 1989-10-25 |
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