JPH073367B2 - Fluorescence polarization measurement device - Google Patents
Fluorescence polarization measurement deviceInfo
- Publication number
- JPH073367B2 JPH073367B2 JP14108985A JP14108985A JPH073367B2 JP H073367 B2 JPH073367 B2 JP H073367B2 JP 14108985 A JP14108985 A JP 14108985A JP 14108985 A JP14108985 A JP 14108985A JP H073367 B2 JPH073367 B2 JP H073367B2
- Authority
- JP
- Japan
- Prior art keywords
- light
- fluorescence
- polarization
- switching element
- phase switching
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000002875 fluorescence polarization Methods 0.000 title claims description 13
- 238000005259 measurement Methods 0.000 title description 7
- 230000010287 polarization Effects 0.000 claims description 45
- 230000005284 excitation Effects 0.000 claims description 18
- 239000013307 optical fiber Substances 0.000 claims description 10
- 238000004458 analytical method Methods 0.000 claims description 5
- 239000000126 substance Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000004973 liquid crystal related substance Substances 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012757 fluorescence staining Methods 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6445—Measuring fluorescence polarisation
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Spectrometry And Color Measurement (AREA)
Description
【発明の詳細な説明】 産業上の利用分野 この発明は蛍光の偏光度によって細胞の状態を測定する
ための蛍光偏光測定装置に関するものであり、この蛍光
偏光測定装置は、蛍光染色等の方法で細胞内の物質に蛍
光を持たせた後、蛍光励起光を直線偏光させて照射し、
物質からの蛍光を測定し偏光のずれ（偏光度）により物
質の状態を調べるものである。TECHNICAL FIELD The present invention relates to a fluorescence polarization measuring apparatus for measuring the state of cells by the degree of polarization of fluorescence, and this fluorescence polarization measuring apparatus can be used for methods such as fluorescence staining. After fluorescence is given to the substance in the cell, the fluorescence excitation light is linearly polarized and irradiated,
It measures the fluorescence from a substance and examines the state of the substance by the deviation of polarization (polarization degree).
従来の技術 一般に、蛍光偏光測定で得られる蛍光偏光度をＰとする
と、Ｐ値は次式で求められる。2. Description of the Related Art Generally, when the fluorescence polarization degree obtained by fluorescence polarization measurement is P, the P value is calculated by the following equation.
ここで、IAは蛍光励起光の偏光方向と平行な方向の蛍光
の偏光成分の強度、IPは蛍光励起光の偏光方向と直角な
方向の蛍光の偏光成分の強度である。 Here, I A is the intensity of the polarization component of fluorescence in the direction parallel to the polarization direction of the fluorescence excitation light, and I P is the intensity of the polarization component of fluorescence in the direction perpendicular to the polarization direction of the fluorescence excitation light.
発明が解決しようとする問題点 偏光度を求めるための蛍光励起光の偏光方向に平行な方
向の蛍光の偏光成分の強度および蛍光励起光の偏光方向
に直角な方向の蛍光の偏光成分の強度を検出するのに、
従来は、励起側の偏光子の偏光方向を固定し、受光側の
検光子を回転させることによって各偏光成分の強度を分
離検出したり、受光側の検光子を固定し、励起側の偏光
子を回転させることにより蛍光励起光の偏光方向を検光
子の検光方向に対して平行と直角に切り換えて、偏光度
を測定していた。Problems to be Solved by the Invention The intensity of the polarization component of fluorescence in the direction parallel to the polarization direction of the fluorescence excitation light and the intensity of the polarization component of fluorescence in the direction perpendicular to the polarization direction of the fluorescence excitation light for determining the polarization degree are described. To detect
Conventionally, the polarization direction of the excitation side polarizer is fixed, and the intensity of each polarization component is separated and detected by rotating the light receiving side analyzer, or the light receiving side analyzer is fixed and the excitation side polarizer is fixed. By rotating the, the polarization direction of the fluorescence excitation light was switched between parallel and perpendicular to the analysis direction of the analyzer, and the polarization degree was measured.
しかし、このような偏光子や検光子を機械的に回転させ
る構成においては、偏光子や検光子を回転させる機械的
な精度が装置全体の性能を左右する上に、装置として大
きくなるとともに製造が容易でないという欠点がある。However, in such a configuration in which the polarizer and the analyzer are mechanically rotated, the mechanical accuracy of rotating the polarizer and the analyzer influences the performance of the entire device, and the device becomes large and is manufactured. It has the drawback of not being easy.
また、偏光子の偏光方向や検光子の検光方向を機械的に
切り換えるのに代えて、例えば液晶,PLZT等の位相切換
素子を利用して偏光子から出た偏光光の偏光方向を電気
的に切り換えることもできるが、偏光子や検光子を機械
的に回転させるのとは異なり、切換によって透過光量が
変化し、精密な装置には使用しがたい欠点があった。Further, instead of mechanically switching the polarization direction of the polarizer or the analysis direction of the analyzer, for example, a phase switching element such as liquid crystal or PLZT is used to electrically change the polarization direction of the polarized light emitted from the polarizer. However, unlike mechanical rotation of the polarizer or analyzer, the amount of transmitted light changes due to the switching, and there is a drawback that it is difficult to use for precision devices.
この発明は、上記の欠点に鑑みてなされたもので、小型
で製造が容易でしかもより精度の高い蛍光偏光測定装置
を提供することを目的とする。The present invention has been made in view of the above drawbacks, and an object of the present invention is to provide a fluorescence polarization measuring apparatus that is small in size, easy to manufacture, and highly accurate.
問題点を解決するための手段 この発明の蛍光偏光測定装置は、蛍光励起光を発する光
源と、この光源の光を直線偏光する偏光子と、この偏光
子から出た光の偏光方向を互いに直交する２方向に切り
換えてサンプルセルに入射させる位相切換素子と、第１
図において紙面と垂直に検光方向が設定され前記サンプ
ルセルから出た蛍光を検光する検光子と、この検光子か
ら出た蛍光を受ける第１の光検出素子と、前記位相切換
素子から出た光を受ける第２の光検出素子と、前記位相
切換素子から出た光を前記第２の光検出素子に導く光フ
ァイバと、前記第２の光検出素子の出力に応じて前記光
源の光量を制御することにより前記光源の位相切換素子
から出る光を一定に制御するフィードバック回路とを備
える構成にしたものである。Means for Solving the Problems The fluorescence polarization measuring apparatus of the present invention comprises a light source that emits fluorescence excitation light, a polarizer that linearly polarizes the light of this light source, and the polarization directions of the light emitted from this polarizer are orthogonal to each other. A phase switching element for switching the light beam into two directions to make it enter the sample cell;
In the figure, an analyzer for detecting fluorescence emitted from the sample cell with an analysis direction set perpendicular to the plane of the paper, a first photodetector element for receiving fluorescence emitted from the analyzer, and an output from the phase switching element Second light detecting element for receiving the light, an optical fiber for guiding the light emitted from the phase switching element to the second light detecting element, and a light amount of the light source according to the output of the second light detecting element. And a feedback circuit that constantly controls the light emitted from the phase switching element of the light source.
上記のように、本発明においては、位相切換素子により
蛍光励起光の偏光方向を互いに異なる２方向に電気的に
切り換えるようにしたため、従来例のように機械的に切
り換えるのに較べ小型化できるとともに製造が容易であ
る。As described above, in the present invention, since the polarization direction of the fluorescence excitation light is electrically switched by the phase switching element to the two different directions, the size can be reduced as compared with the mechanical switching as in the conventional example. Easy to manufacture.
しかも、位相切換素子から出た光を検出して光源の光量
を制御することにより位相切換素子から出る光の光量を
一定に制御する構成で、位相切換素子の電気的切換によ
る位相切換素子の透過光量の違いによる測定誤差を解消
しているため、高精度に測定することができる。特に、
位相切換素子から出た光を光ファイバによって第２の光
検出素子に導いているため、第２の光検出素子の偏光特
性の影響を受けることなく定光量制御を行うことができ
る。Moreover, by detecting the light emitted from the phase switching element and controlling the light quantity of the light source, the light quantity of the light emitted from the phase switching element is controlled to be constant, and the transmission of the phase switching element by the electrical switching of the phase switching element is transmitted. Since the measurement error due to the difference in the light quantity is eliminated, the measurement can be performed with high accuracy. In particular,
Since the light emitted from the phase switching element is guided to the second photodetection element by the optical fiber, it is possible to perform the constant light amount control without being affected by the polarization characteristic of the second photodetection element.
実施例 この発明の一実施例を第１図および第２図に基づいて説
明する。この蛍光偏光測定装置は、第１図に示すよう
に、水銀ランプ，ハロゲンランプ，キセノンランプ等の
光源１より出た光を集光レンズ２より平行光にし、この
平行光を波長選択フィルタ３を通すことで蛍光励起光を
抽出し、さらに偏光子４を用いて直線偏光の蛍光励起光
にするようになっている。位相切換素子５は、例えば液
晶やPLZTのような素子からなり、電極に直流電圧が比較
的長い切換周期で断続印加され、偏光子４を通して入射
する直線偏光の蛍光励起光を電圧印加状態ではそのまま
通過させ、非電圧印加状態では偏光方向を90度回転させ
て通過させるようになっている。Embodiment An embodiment of the present invention will be described with reference to FIGS. 1 and 2. In this fluorescence polarization measuring apparatus, as shown in FIG. 1, light emitted from a light source 1 such as a mercury lamp, a halogen lamp, a xenon lamp is collimated by a condenser lens 2, and the collimated light is converted by a wavelength selection filter 3. The fluorescence excitation light is extracted by passing it through, and is further converted into linearly polarized fluorescence excitation light by using the polarizer 4. The phase switching element 5 is composed of, for example, an element such as liquid crystal or PLZT, and a DC voltage is intermittently applied to the electrodes with a relatively long switching period, and linearly polarized fluorescence excitation light incident through the polarizer 4 remains unchanged in a voltage applied state. The light is passed through, and the polarization direction is rotated by 90 degrees when no voltage is applied, and the light is passed through.
上記位相切換素子５は、電圧印加状態と非電圧印加状態
とによって偏光光の透過率が１％程度変化して蛍光励起
光の強度が変化するので、以下に述べる構成によって位
相切換素子による偏光方向の切り換えによる蛍光励起光
の強度変化を防止するようになっている。In the phase switching element 5, the transmittance of the polarized light changes by about 1% depending on the voltage applied state and the non-voltage applied state, and the intensity of the fluorescence excitation light changes. Therefore, the polarization direction by the phase switching element is changed by the configuration described below. The change in the intensity of the fluorescence excitation light due to the switching of is prevented.
ハーフミラー等を使用して光検出素子に入る光源からの
光をフィードバックして光度の安定化を図る方法は、本
発明における偏光光学系では使用できない。それは、光
検出素子が偏光特性を持つためである。そのため、本実
施例の偏光光学系では、『フィードバック用偏光光を一
度光ファイバを通過させると反射の繰り返しによって偏
光特性が解消した光が光ファイバの出力端に得られる』
という性質を利用して、一旦光ファイバ11を通して光検
出素子12に蛍光励起光の一部を入射させ、光検出素子12
の出力をフィードバック回路13に加えることによりフィ
ードバック回路13がフィードバック量に応じて光源１の
電源14を制御することにより、光源１の光量を制御して
位相切換素子５から出る光の光量を常に一定に保つよう
にしている。これにより、電気制御による位相切換素子
（液晶,PLZTなど）５の切換誤差を解消させるようにな
っている。この際、フィードバック回路13は、光検出素
子12の出力電流を電圧変換して適正増幅の後、電源（市
販のスイッチング電源、例えば、ネミックλ社製のHR−
11−12）１の電圧制御端子に加えることにより光源１の
光量制御を行うようになっている。The method of feeding back the light from the light source entering the photodetector using a half mirror or the like to stabilize the luminous intensity cannot be used in the polarization optical system of the present invention. This is because the photodetector has a polarization characteristic. Therefore, in the polarization optical system of the present embodiment, "the light whose polarization characteristics have been eliminated by repeating reflection once the polarized light for feedback is passed through the optical fiber is obtained at the output end of the optical fiber".
By utilizing this property, a part of the fluorescence excitation light is once made incident on the photodetector 12 through the optical fiber 11, and
The feedback circuit 13 controls the power source 14 of the light source 1 in accordance with the feedback amount by controlling the light amount of the light source 1 so that the light amount of the light emitted from the phase switching element 5 is always constant. I try to keep it. As a result, the switching error of the phase switching element (liquid crystal, PLZT, etc.) 5 due to electrical control is eliminated. At this time, the feedback circuit 13 voltage-converts the output current of the photodetector 12 and appropriately amplifies it, and then supplies the power (commercially available switching power supply, for example, Nemic λ HR-
11-12) The light quantity of the light source 1 is controlled by adding it to the voltage control terminal of 1.
そして、上記のようにして光量が一定に制御され、かつ
直線偏光された蛍光偏光光がサンプルセル６に入射する
ことになる。Then, as described above, the amount of light is controlled to be constant, and the linearly polarized fluorescence polarized light enters the sample cell 6.
サンプルセル６中の試料から出た側方（直角）散乱光と
螢光，ラマン光等は、波長選択フィルタ７を通すことに
よって物質による蛍光のみが抽出され、抽出された蛍光
が偏光子４の偏光方向と平行な偏光方向の検光子８を通
して集光レンズ９で集光されて光電子増倍管などの光検
出素子10に入射することになる。この結果、光検出素子
10の出力には、電圧印加状態では、偏光子４の偏光方向
と平行な方向の蛍光光の偏光成分に対応したものが現わ
れ、非電圧印加状態では、偏光子４の偏光方向と直角な
方向の蛍光光の偏光方向成分に対応したものが現われ
る。With respect to the lateral (right angle) scattered light, fluorescence, Raman light, etc. emitted from the sample in the sample cell 6, only the fluorescence due to the substance is extracted by passing through the wavelength selection filter 7, and the extracted fluorescence of the polarizer 4 is extracted. The light is condensed by the condenser lens 9 through the analyzer 8 having the polarization direction parallel to the polarization direction and is incident on the photodetection element 10 such as a photomultiplier tube. As a result, the photodetector
In the output of 10, when the voltage is applied, what corresponds to the polarization component of the fluorescent light in the direction parallel to the polarization direction of the polarizer 4 appears, and when the voltage is not applied, the direction perpendicular to the polarization direction of the polarizer 4 appears. A component corresponding to the polarization direction component of the fluorescent light appears.
なお、検光子８の検光方向は、第１図において紙面に垂
直でなければならない。It should be noted that the light analysis direction of the analyzer 8 must be perpendicular to the paper surface in FIG.
第２図は、光検出素子10からの測定出力波形で、光強度
を縦軸に、時間を横軸に取ったものである。光検出素子
10の出力として得られる光強度Ｉは、電圧印加状態、す
なわち偏光子４の偏光方向と平行な方向の蛍光の偏光方
向成分が入射されたときにIAとなり、非電圧印加状態、
すなわち偏光子４の偏光方向と直角な方向の蛍光の偏光
方向成分が入射されたときにIBとなり、得られた光強度
IA，IBを元にして第（１）式の演算を行えば、物質の状
態を調べることができる。FIG. 2 shows a measured output waveform from the photodetector 10, in which the light intensity is plotted on the vertical axis and the time is plotted on the horizontal axis. Photo detector
The light intensity I obtained as the output of 10, the voltage application state, i.e. I A becomes when the polarization direction component of the fluorescence polarization direction parallel to the direction of the polarizer 4 is incident, non-voltage applied state,
That next I B when the polarization direction component of the fluorescence polarization direction perpendicular to the direction of the polarizer 4 is incident, obtained light intensity
The state of the substance can be investigated by performing the calculation of the equation (1) based on I A and I B.
このように、この実施例の蛍光偏光測定装置によれば、
位相切換素子５から出た光ファイバ11を通してフィード
バックすることにより位相切換素子５から出る光の光量
を常に一定に制御するようにしたため、位相切換素子５
の透過光量が電圧印加状態と非電圧印加状態とで変化し
てもその光量変化を補正して位相切換素子から出る光の
光量を常に一定にすることができ、高い精度で測定を行
うことが可能となる。しかも、フィードバックする光
は、光ファイバ11を通すことで偏光を解消しているの
で、光検出素子12が偏光特性を有していても全く問題な
い。Thus, according to the fluorescence polarization measuring apparatus of this example,
Since the amount of light emitted from the phase switching element 5 is always controlled to be constant by feedback through the optical fiber 11 output from the phase switching element 5, the phase switching element 5
Even if the amount of transmitted light changes depending on whether the voltage is applied or not applied, the change in the amount of light can be corrected and the amount of light emitted from the phase switching element can be made constant at all times, making it possible to perform measurement with high accuracy. It will be possible. Moreover, since the light to be fed back is depolarized by passing through the optical fiber 11, there is no problem even if the photodetector 12 has a polarization characteristic.
以上の結果、偏光度Ｐを迅速にかつ高精度に測定するこ
とが可能になり、偏光方向を機械的に切り換える場合に
較べて小型で製造の容易な故障の少ない測定器を作るこ
とができる。As a result, it is possible to measure the polarization degree P quickly and with high accuracy, and it is possible to make a measuring instrument that is small in size and easy to manufacture and has few failures as compared with the case of mechanically switching the polarization direction.
次に、試料からの出力は検光子８の偏光方向を固定して
側方（直角）散乱光を捉えることにより光検出素子（光
電子増倍管）10の一方向のみの偏光特性を利用できるの
で、光検出素子による偏光誤差を避けることができる。Next, the output from the sample can use the polarization characteristic of only one direction of the photodetector (photomultiplier tube) 10 by fixing the polarization direction of the analyzer 8 and capturing the side (right angle) scattered light. It is possible to avoid polarization error due to the photodetector.
発明の効果 この発明の蛍光偏光測定装置によれば、位相切換素子に
より蛍光励起光の偏光方向を互いに異なる２方向に電気
的に切り換えるようにしたため、従来例のように機械的
に切り換えるのに較べ小型化できるとともに製造が容易
である。EFFECTS OF THE INVENTION According to the fluorescence polarization measuring apparatus of the present invention, since the polarization direction of the fluorescence excitation light is electrically switched to the two different directions by the phase switching element, compared to the mechanical switching as in the conventional example. It can be miniaturized and easy to manufacture.
しかも、位相切換素子から出た光を検出して光源の光量
を制御することにより位相切換素子から出る光の光量を
一定に制御する構成で、位相切換素子の電気的切換によ
る位相切換素子の透過光量の違いによる測定誤差を解消
しているため、高精度に測定することができる。特に、
位相切換素子から出た光を光ファイバによって第２の光
検出素子に導いているため、第２の光検出素子の偏光特
性の影響を受けることなく定光量制御を行うことができ
る。Moreover, by detecting the light emitted from the phase switching element and controlling the light quantity of the light source, the light quantity of the light emitted from the phase switching element is controlled to be constant, and the transmission of the phase switching element by the electrical switching of the phase switching element is transmitted. Since the measurement error due to the difference in the light quantity is eliminated, the measurement can be performed with high accuracy. In particular,
Since the light emitted from the phase switching element is guided to the second photodetection element by the optical fiber, it is possible to perform the constant light amount control without being affected by the polarization characteristic of the second photodetection element.
第１図はこの発明の一実施例の構成を示す構成図、第２
図はその出力波形図である。 １…光源、２…集光レンズ、３…波長選択フィルタ、４
…偏光子、５…位相切換素子、６…サンプルセル、７…
波長選択フィルタ、８…検光子、９…集光レンズ、10…
光検出素子、11…光ファイバ、12…光検出素子、13…フ
ィードバック回路、14…電源FIG. 1 is a block diagram showing the configuration of an embodiment of the present invention, and FIG.
The figure is the output waveform diagram. 1 ... Light source, 2 ... Condensing lens, 3 ... Wavelength selection filter, 4
... Polarizer, 5 ... Phase switching element, 6 ... Sample cell, 7 ...
Wavelength selection filter, 8 ... Analyzer, 9 ... Condensing lens, 10 ...
Photodetector, 11 ... Optical fiber, 12 ... Photodetector, 13 ... Feedback circuit, 14 ... Power supply
Claims (1)
を直線偏光する偏光子と、この偏光子から出た光の偏光
方向を互いに直交する２方向に切り換えてサンプルセル
に入射させる位相切換素子と、この位相切換素子から出
た光の偏光方向のいずれか一方と平行に検光方向が設定
され前記サンプルセルから出た蛍光を検光する検光子
と、この検光子から出た蛍光を受ける第１の光検出素子
と、前記位相切換素子から出た光を受ける第２の光検出
素子と、前記位相切換素子から出た光を前記第２の光検
出素子に導く光ファイバと、前記第２の光検出素子の出
力に応じて前記光源の光量を制御することにより前記位
相切換素子から出る光の光量を一定に制御するフィード
バック回路とを備えた蛍光偏光測定装置。1. A light source that emits fluorescence excitation light, a polarizer that linearly polarizes the light of this light source, and a phase that makes the light emitted from this light source enter a sample cell by switching between two polarization directions orthogonal to each other. A switching element, an analyzer for detecting fluorescence emitted from the sample cell in which an analysis direction is set in parallel with one of polarization directions of light emitted from the phase switching element, and fluorescence emitted from the analyzer. A first photodetecting element for receiving the light, a second photodetecting element for receiving the light emitted from the phase switching element, and an optical fiber for guiding the light emitted from the phase switching element to the second photodetecting element. A fluorescence polarization measuring apparatus, comprising: a feedback circuit that controls the light amount of the light source to be constant according to the output of the second photodetector to control the light amount of the light emitted from the phase switching device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14108985A JPH073367B2 (en) | 1985-06-27 | 1985-06-27 | Fluorescence polarization measurement device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14108985A JPH073367B2 (en) | 1985-06-27 | 1985-06-27 | Fluorescence polarization measurement device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS622128A JPS622128A (en) | 1987-01-08 |
| JPH073367B2 true JPH073367B2 (en) | 1995-01-18 |
Family
ID=15283945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14108985A Expired - Lifetime JPH073367B2 (en) | 1985-06-27 | 1985-06-27 | Fluorescence polarization measurement device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH073367B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL88571A (en) * | 1988-12-02 | 1998-06-15 | Ormat Turbines 1965 Ltd | Method of and apparatus for producing power using steam |
| FI20031294A0 (en) * | 2003-09-10 | 2003-09-10 | Thermo Labsystems Oy | Fluorometer calibration |
| JP4749805B2 (en) * | 2005-09-05 | 2011-08-17 | オリンパスメディカルシステムズ株式会社 | Raman scattered light observation system |
| CN117214140B (en) * | 2022-06-02 | 2026-04-21 | 深圳迈瑞生物医疗电子股份有限公司 | A specific protein analysis system |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5834768B2 (en) * | 1972-09-20 | 1983-07-28 | キヤノン株式会社 | Henkojiyokiyobunkouki |
| JPS5356078A (en) * | 1976-10-30 | 1978-05-22 | Nippon Bunko Kogyo Kk | Poralized fluorescence measuring apparatus |
| JPS56130619A (en) * | 1980-03-19 | 1981-10-13 | Mitsubishi Electric Corp | Photodetecting device |
| US4412744A (en) * | 1981-06-01 | 1983-11-01 | E. I. Du Pont De Nemours & Co. | Absolute spectrophotometer |
| JPS58156838A (en) * | 1982-03-15 | 1983-09-17 | Hitachi Ltd | Zeeman atomic absorptiometer |
-
1985
- 1985-06-27 JP JP14108985A patent/JPH073367B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS622128A (en) | 1987-01-08 |
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