JPH0743373B2 - Sputum collecting cell screening method - Google Patents
Sputum collecting cell screening methodInfo
- Publication number
- JPH0743373B2 JPH0743373B2 JP16261289A JP16261289A JPH0743373B2 JP H0743373 B2 JPH0743373 B2 JP H0743373B2 JP 16261289 A JP16261289 A JP 16261289A JP 16261289 A JP16261289 A JP 16261289A JP H0743373 B2 JPH0743373 B2 JP H0743373B2
- Authority
- JP
- Japan
- Prior art keywords
- sputum
- glass plate
- cells
- pack
- collection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 206010036790 Productive cough Diseases 0.000 title claims description 29
- 210000003802 sputum Anatomy 0.000 title claims description 29
- 208000024794 sputum Diseases 0.000 title claims description 29
- 238000000034 method Methods 0.000 title claims description 10
- 238000012216 screening Methods 0.000 title description 7
- 239000011521 glass Substances 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 14
- 229920003002 synthetic resin Polymers 0.000 claims description 7
- 239000000057 synthetic resin Substances 0.000 claims description 7
- 239000011148 porous material Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 4
- 238000004043 dyeing Methods 0.000 claims 1
- 239000000834 fixative Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 238000003860 storage Methods 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 description 4
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000011111 cardboard Substances 0.000 description 3
- 201000005202 lung cancer Diseases 0.000 description 3
- 208000020816 lung neoplasm Diseases 0.000 description 3
- -1 polyethylene Polymers 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 230000032258 transport Effects 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 239000005844 Thymol Substances 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000000123 paper Substances 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 229960000790 thymol Drugs 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 208000009956 adenocarcinoma Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000011087 paperboard Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920013716 polyethylene resin Polymers 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 208000000649 small cell carcinoma Diseases 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 206010041823 squamous cell carcinoma Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229920001169 thermoplastic Polymers 0.000 description 1
- 239000004416 thermosoftening plastic Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 230000002618 waking effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は肺癌検診など喀痰細胞診をするために用いられ
る喀痰集細胞検診方法に関するものである。TECHNICAL FIELD The present invention relates to a sputum collection cell screening method used for sputum cytology such as lung cancer screening.
一般に、肺癌の集団検診における喀痰細胞診として集細
胞法が広く用いられていて、従来の集細胞法では、各被
検者が家庭内などで任意連日、例えば３日間、早朝痰を
固定液の入れてある喀痰容器に蓄痰し、細胞を固定した
まま保存したものを臨床検査室などの検査センターまで
輸送または郵送し、遠心沈殿法で、即ちブレンダーまた
はホモジナイザーで喀痰を切断し、遠心分離することに
よる沈渣で集細胞しているが、この遠心分離機のブレン
ダーまたはホモジナイザーは高速回転であるため、細胞
の物理的変性と結合性の機械的な破壊を来し、癌細胞と
判定した場合でも、組織型、すなわち扁平上皮癌，腺
癌，小細胞癌のいずれであるかを決め難い場合が多いこ
とが知られている。Generally, a cell collecting method is widely used as a sputum cytology in a mass screening for lung cancer. In the conventional cell collecting method, each subject is treated with fixative solution of early morning sputum for any consecutive days, such as at home, for 3 days. Sputum is stored in the sputum container that has been stored, and cells that have been fixed and stored are transported or mailed to a laboratory such as a clinical laboratory, and the sputum is cut by a centrifugal precipitation method, that is, using a blender or homogenizer, and centrifuged. Although the cells are collected in the sediment, the blender or homogenizer of this centrifuge rotates at high speed, which causes physical denaturation of cells and mechanical destruction of binding properties, and even when judged as cancer cells. It is known that it is often difficult to determine the tissue type, that is, squamous cell carcinoma, adenocarcinoma, or small cell carcinoma.
ところが、この従来の方法では、高価な遠心分離機が必
要不可欠となり、その取扱いもやっかいで、特に遠心分
離機に喀痰を移しかえ必要から、１度に１検体しか行う
ことができず、大量の検体を扱う施設では、処理に困難
を来すし、また個々の検体を処理するごとに検体間の細
胞の混入を防ぐため、ブレンダーまたはホモジナイザー
を完全に洗滌する必要があり、減菌処理や検体処理上、
著しく煩雑で問題があった。However, in this conventional method, an expensive centrifuge is indispensable, and its handling is troublesome. Especially, since it is necessary to transfer sputum to the centrifuge, only one sample can be performed at a time, and a large amount of it is required. In a facility that handles specimens, the blender or homogenizer must be thoroughly washed to prevent the contamination of cells between specimens every time each specimen is treated. Up,
It was extremely complicated and problematic.
本発明では、これら従来の欠点を適確に排除しようとす
るもので、遠心分離機を用いることなく細胞変性が起き
ないように固定液中で保護させると同時に細胞分離も容
易で検診操作の簡便な喀痰細胞検診方法を提供すること
を目的としたものである。In the present invention, it is intended to properly eliminate these conventional drawbacks, and without the use of a centrifuge, the cells are protected in a fixative solution so that cell denaturation does not occur, and at the same time, the cells can be easily separated and the examination operation is simple. The purpose of the present invention is to provide a method for screening sputum cells.
本発明は、サコマノ液などの保存固定液に蓄痰した集収
パックを検査センターなどの指定機関に輸送または郵送
し、該集収パックを振盪溶解操作を施したのち、集収パ
ック内の懸濁液を平均孔径５〜20μの目の細かい多孔質
合成樹脂材上に流展して液を吸収分離し、材表面上
に残った表面細胞をガラス板で掻き採ってスライドグラ
ス板に伸展して固定し染色後に鏡検することを特徴とす
る喀痰集細胞検診方法である。The present invention transports or mails a collection pack that has been stored in a storage fixative such as Sacomano solution to a designated institution such as an inspection center, shakes and dissolves the collection pack, and then suspends the suspension in the collection pack. Liquid is absorbed and separated by spreading on a fine porous synthetic resin material with an average pore size of 5 to 20μ, and the surface cells remaining on the surface of the material are scraped with a glass plate and extended and fixed on a slide glass plate. It is a method for detecting sputum collecting cells, which is characterized by microscopic examination after staining.
本発明の喀痰集細胞検診方法では、患者など被検診者に
起床時に口の中を水でゆすいでから朝痰を集収パックに
採る。この場合、保存固定液入りのポリエチレン製の口
をハサミで切りとり、この袋口から吐き入れるか、別容
器から適量をハシで挟むか捕集ネットですくい取って袋
内に投入し、例えば３日間乃至５日間の痰を採って蓄痰
してから空気を押し出して袋口をラベルと共に折り曲げ
止口具で封入し、紙函に納めて必要に応じチャックポリ
袋に入れて厚紙封筒で指定機関に郵送又は輸送（持参を
も含む）し、この検査センターでこの集収パックを約一
日間振盪して、溶解操作を施したのち、集収パック内の
懸濁液を吸液性の多孔質合成樹脂材上に流展して液を
吸収分離し、該材表面上に残った表面細胞をガラス板
で掻き採ってスライドグラス板に伸展して塗抹し、乾燥
させて固定してから染色したのち顕微鏡で鏡検して集細
胞の検診を能率よく行うものである。In the method for screening sputum collecting cells of the present invention, the mouth of a patient to be examined such as a patient is rinsed with water when waking up, and then the sputum is collected in a collection pack. In this case, cut the polyethylene mouth containing the storage fixative with scissors and spit it out from this bag mouth, or pinch an appropriate amount from another container with a hash or scoop it with a collection net and put it in the bag, for example for 3 days After collecting sputum for 5 days and storing the sputum, air is pushed out, the bag mouth is folded with a label and enclosed with a stopper, put in a paper box, put in a zipper polybag if necessary, and sent to a designated engine with a cardboard envelope. After mailing or transporting (including bringing), shake this collection pack at this inspection center for about 1 day to dissolve it, and then dissolve the suspension in the collection pack, and absorb the suspension in the collection pack. It is spread on the surface to absorb and separate the liquid, the surface cells remaining on the surface of the material are scraped off with a glass plate, spread on a slide glass plate, smeared, dried and fixed, and then stained with a microscope. Those that efficiently perform microscopic examination of collected cells A.
本発明の実施例を第１〜２図例を参照して説明すると、
サコマノ液などの保存固定液１を入れたポリエチレン樹
脂などの合成樹脂製袋の集収パック２内に蓄痰したの
ち、袋口21を採痰日，氏名などの記入をする記録ラベル
３と共に折り曲げ止口具４で封入し、該集収パック２を
組立容器、例えば紙凾その他の扁平容器、或いは厚紙袋
に納め必要に応じ外箱６又はチャックポリ袋などの外袋
に挿入して梱包し、厚紙封筒（図示せず）で検査センタ
ーなどの指定機関に郵送又は輸送（持参をも含む）し、
この送られて来た集収パック２を検査センターで約１日
間振盪溶解操作を施したのち、集収パック２内の懸濁液
Ａを吸液性の多孔質体又は発泡合成樹脂材７例えば平
均孔径５〜20μ、好ましくは８〜15μ，気孔率80〜95％
の板状材上に流展して液分を吸収分離し、該材表面
上に残った表面細胞Ｂをガラス板８で掻き採ってスライ
ドグラス板９に移し、細胞を圧し潰すように２枚のスラ
イドグラス板9,9で摺り合わせて伸展して塗抹し、自然
乾燥又はファンなど強制乾燥でその固定化を行いパパニ
コロウなどの染料で染色Ｃ′したのち、該スライドグラ
ス板９を顕微鏡10にセットして鏡検して集細胞の検診を
行えるようにしてある。An embodiment of the present invention will be described with reference to FIGS.
After storing sputum in a collecting pack 2 made of a synthetic resin bag such as polyethylene resin containing a storage fixative solution 1 such as sacomano solution, fold the bag mouth 2 1 together with a record label 3 on which the sputation date, name, etc. are written. It is sealed with a stopper 4, and the collection pack 2 is packed in an assembly container, for example, a paper container or other flat container, or a cardboard bag, and if necessary, inserted into an outer box 6 or an outer bag such as a zipper polybag, and packed. Use a cardboard envelope (not shown) to mail or transport (including bringing) to designated organizations such as inspection centers.
The collected collection pack 2 is shaken and dissolved at the inspection center for about 1 day, and then the suspension A in the collection pack 2 is absorbed into a liquid-absorbing porous body or a foamed synthetic resin material 7, for example, an average pore diameter. 5-20μ, preferably 8-15μ, porosity 80-95%
Of the surface cells B remaining on the surface of the material is scraped off by the glass plate 8 and transferred to the slide glass plate 9, and the cells are pressed and crushed into two pieces. The slide glass plates 9 and 9 are slid on each other, spread and smeared, fixed by natural drying or forced drying with a fan, and dyed with a dye such as Papanicolaou C ', and then the slide glass plate 9 is placed on the microscope 10. It is set and microscopically examined so that the collected cells can be examined.
この場合、前記保存固定液１としては、サコマノ液（蛋
白分解酸素使用）そのほかに、エタノール，ポリエチレ
ングリコールまたはメタノール，チモール、またはホル
マリン上より成るものを用いてあるが、例えばエチルア
ルコール50％，チモール１％，カーボワックス２％，生
理食塩水残部の組合せの保存液を集収パック２に適量充
填して蓄痰しやすいようにするのがよい。In this case, as the storage fixative 1, sacomano solution (using proteolytic oxygen), as well as ethanol, polyethylene glycol or methanol, thymol, or formalin is used. For example, ethyl alcohol 50%, thymol It is advisable to fill the collecting pack 2 with an appropriate amount of a preservative solution containing a combination of 1%, carbowax 2%, and the rest of physiological saline to facilitate the storage of sputum.
また、紙凾５は止口具４で袋口をグリップした集収パッ
ク２をそのまま、或いは折り曲げて収納できるトレー状
のケースで一端部51がヒンジ部で開閉自在に可動となっ
ていて、集収パック２の差入れ，取出しやすい挿入口が
できるものを用いてあり、指差込用の透孔62のある開閉
蓋61,61を備えた外箱６に差込形式で嵌挿して梱包でき
るようにしてあるが、外箱６と内箱との組合せに限ら
ず、前記集収パック２を保護できる比較的扁平なケース
なら単一のものでもよい。Further, Kami凾5 as a collection pack 2 that grips the bag mouth by the locking opening device 4, or one end portion 5 1 in the tray-shaped case can be stored by bending is not opened and closed freely movable hinge part, collected Packing is done by inserting the pack 2 into the outer box 6 which has an opening / closing lid 6 1 , 6 1 with a through hole 6 2 for inserting a finger. Although it can be done, the case is not limited to the combination of the outer box 6 and the inner box, and a single case may be used as long as it is a relatively flat case that can protect the collecting pack 2.
さらに前記多孔質合成樹脂材７としては、ポリビニル
ホルマールを基質とする発泡体、或いはポリエチレン，
ポリスチレン，ポリオレフィン，ポリ塩化ビニル，ポリ
プロピレンなどの熱可塑性合成樹脂で目の細かいスポン
ジ状乃至多孔質状に形成したものを用い、板状材とし
て吸水性が強く湿潤状態では膨潤して耐薬品性に優れた
ものを用いるのがよく、平均孔径８〜12μのもので気孔
率は80〜95％、好ましくは84〜90％のものを用いるのが
よい。Further, as the porous synthetic resin material 7, a foamed material using polyvinyl formal as a substrate, or polyethylene,
Using a sponge-like or porous material made of thermoplastic synthetic resin such as polystyrene, polyolefin, polyvinyl chloride, polypropylene, etc., it has a strong water absorption as a plate-like material and swells in a wet state to have chemical resistance. It is preferable to use an excellent one having an average pore size of 8 to 12 μm and a porosity of 80 to 95%, preferably 84 to 90%.
なお、被検者には前記集収パック２を入れた紙凾５と外
箱６とを郵送用封筒とともに、記録ラベル３と使用説明
書，問診票などを組み合わせて渡し、蓄痰させて郵送又
は輸送させればよいが、必要に応じ材７または／及ガ
ラス板８とともに喀痰捕集ネット13を組込んだ形態で販
売することもできる。It should be noted that the subject 5 and the outer box 6 containing the collection pack 2 together with the mailing envelope, the record label 3, the instruction manual, the questionnaire, etc. are handed over to the subject, and they are then stored and sent by mail. Although it may be transported, it may be sold in a form in which a sputum collection net 13 is incorporated together with the material 7 and / or the glass plate 8 if necessary.
さらに、痰にはだ液がかなり大量に含まれていることが
多いので、被検者が集痰する際には第３図に示すように
ネット11を張ったスプーン12からなる喀痰捕集ネット13
で朝痰をすくい採り、或いはネット上に吐き出し、だ液
をネット11でして分離してから痰のみを集収パック２
に適確に蓄痰できるようにするのが無駄がなくて良い。
ネット11は寒冷秒＃200（2.10mm）のメッシュのものが
最適であった。またこの喀痰捕集ネットを用いることに
よって検査有効資料率が30％向上した。Furthermore, since sputum often contains a large amount of saliva, when a subject collects sputum, a sputum collection net consisting of a spoon 12 covered with a net 11 as shown in FIG. 13
Squeeze the sputum with, or spit it on the net, separate the saliva with the net 11 and collect the sputum only. Pack 2
There is no need to waste so that it can store sputum properly.
Net 11 had a cold second # 200 (2.10 mm) mesh. In addition, the use of this sputum collection net improved the effective inspection data rate by 30%.
〔発明の効果〕 本発明は、保存固定液に蓄痰した集収パックを検査セン
ターなどの指定機関に輸送または郵送し、該集収パック
を振盪溶解操作を施したのち、集収パック内の懸濁液を
平均孔径５〜20μの目の細かい多孔質合成樹脂材上に
流展して液を吸収分離し、材表面上に残った表面細胞
をガラス板で掻き採ってスライドグラス板に伸展して固
定し染色後に鏡検することにより、細胞診に遠心分離機
などを用いることなく、従来の煩雑な操作方法を著しく
簡略化し、製作時間も大幅に短縮することができ、肺癌
検査をより短時間に、大量に処理でき、試薬は毒性がな
く安定であるため、病院内での入院患者者や外来患者の
肺癌検査などのほか集団検診における作業を容易に適確
化できるなどの効果がある。[Effects of the Invention] The present invention transports or mails a collection pack containing sputum stored in a storage fixative to a designated institution such as an inspection center, and shakes and dissolves the collection pack, and then the suspension in the collection pack. Is spread on a fine porous synthetic resin material with an average pore diameter of 5 to 20μ to absorb and separate the liquid, and the surface cells remaining on the material surface are scraped with a glass plate and spread and fixed on a slide glass plate. By performing a microscopic examination after staining, the conventional complicated operation method can be significantly simplified without using a centrifuge for cytodiagnosis, and the production time can be significantly shortened. Since it can be processed in a large amount and the reagent is stable without toxicity, it has the effect of easily optimizing the work in group medical examinations such as lung cancer examination of inpatients and outpatients in hospitals.
図面は本発明の実施例を示し、第１図は集細胞用具の斜
視図、第２図は検診の系統説明図、第３図は喀痰捕集ネ
ットの斜視図である。 Ａ……懸濁液、Ｂ……表面細胞、Ｃ……染色、１……保
存固定液、２……集収パック、３……記録ラベル、４…
…止口具、５……紙凾、６……外箱、７……材、８…
…ガラス板、９……スライドグラス板、10……顕微鏡、
11……ネット、12……スプーン、13……喀痰捕集ネッ
ト。The drawings show an embodiment of the present invention. Fig. 1 is a perspective view of a cell collecting tool, Fig. 2 is a system explanatory view of screening, and Fig. 3 is a perspective view of a sputum collecting net. A ... Suspension, B ... Surface cells, C ... Staining, 1 ... Preservative fixative, 2 ... Collection pack, 3 ... Recording label, 4 ...
… Stoppers, 5 …… Paper board, 6 …… Outer box, 7 …… Material, 8…
… Glass plate, 9 …… Slide glass plate, 10 …… Microscope,
11 …… Net, 12 …… Spoon, 13 …… Sputum collection net.
Claims (1)
収パックを検査センターなどの指定機関に輸送または郵
送し、該集収パックを振盪溶解操作を施したのち、集収
パック内の懸濁液を平均孔径５〜20μの目の細かい多孔
質合成樹脂材上に流展して液を吸収分離し、材表面
上に残った表面細胞をガラス板で掻き採ってスライドグ
ラス板に伸展して固定し染色後に鏡検することを特徴と
する喀痰集細胞検診方法。1. A collection pack that has been stored in a fixed solution such as sacomano solution is transported or mailed to a designated institution such as an inspection center, and the collection pack is shaken and dissolved, and then suspended in the collection pack. Is spread on a fine porous synthetic resin material with an average pore diameter of 5 to 20μ to absorb and separate the liquid, and the surface cells remaining on the material surface are scraped with a glass plate and spread and fixed on a slide glass plate. A method for detecting sputum collecting cells, which comprises performing a microscopic examination after dyeing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16261289A JPH0743373B2 (en) | 1989-06-27 | 1989-06-27 | Sputum collecting cell screening method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16261289A JPH0743373B2 (en) | 1989-06-27 | 1989-06-27 | Sputum collecting cell screening method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0328764A JPH0328764A (en) | 1991-02-06 |
| JPH0743373B2 true JPH0743373B2 (en) | 1995-05-15 |
Family
ID=15757909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16261289A Expired - Lifetime JPH0743373B2 (en) | 1989-06-27 | 1989-06-27 | Sputum collecting cell screening method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0743373B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006129761A1 (en) * | 2005-06-03 | 2006-12-07 | Nichirei Biosciences Inc. | Method of collecting biological excretory substance |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010105975A (en) * | 2000-05-19 | 2001-11-29 | 이원배 | Cytalogy auto smear method |
| CN102375069A (en) * | 2010-08-26 | 2012-03-14 | 南京神州英诺华医疗科技有限公司 | Novel automatic treatment instrument for respiratory tract specimen |
-
1989
- 1989-06-27 JP JP16261289A patent/JPH0743373B2/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006129761A1 (en) * | 2005-06-03 | 2006-12-07 | Nichirei Biosciences Inc. | Method of collecting biological excretory substance |
| JP4796058B2 (en) * | 2005-06-03 | 2011-10-19 | 株式会社ニチレイバイオサイエンス | How to collect biological waste |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0328764A (en) | 1991-02-06 |
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