JPH0748215A - Nematode - Google Patents

Abstract

PURPOSE: To obtain a nematode used for biologically controlling an insecticide such as termite. CONSTITUTION: An insecticidal amount of Termite Nematodes in infection stage is brought into contact with a termite of the genus Reticulitermes selected from a group comprising R. flavipes and R. Vivginicus to control the termite. Infectious Termite Nematodes are preferably applied with a carrier selected from a group comprising oil, wax, hydrogel, water thickener and other gelling agents and/or termite trial-following pheromone such as 4-phenyl-3-butene-1-ol or (Z,Z)-3,6-dodecadiene-1-ol. Termite Nematodes is isolated from Reticulitermes flavipes which is a termite discovered in South Florida and Termite Nematodes is a new species of new genus distinguishable from the genus Steinernema by characteristics of male and female and infectious larva.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to means for biologically controlling pests such as termites. More specifically,
The present invention is a reticle
mes) a new genus (ng) and a new species (n.so.) of nematodes for use in the control of genus termites.

[0002]

Brief Description of the Prior Art In view of the safety and environmental concerns of using synthetic pesticides, there is an increasing need to use biological means such as nematodes as insecticides. Poinar, G .; O. , Jr. "Nematodes for biological control of reptiles" (Nematodes)
for Biological Control of
Insects), CRC Press, Inc. B
See oca Raton, Florida (1979). U.S. Pat. No. 4,178,366 describes a nematode, Neoaplectana carpocapsae.
An insecticidal composition comprising an infectious larva of Ctana carpocapsae) is disclosed.

[0003] Courtney, W .; D. , D.
Polley and V.I. I. Miller, 1955,
TAF "Improved fixative in nematode technology", Pla
ntDisease Reporter 39: 570
-571; Doucet, M .; M. A. 1986 "Co
New Neo-Aplectana Stainer from Rdoba and Argentina
tainer), 1929 (Nematoda: st
einenematidae) ", Revue de
Nematologie 9: 317-323; Do
ucet, M .; M. A. And M.M. E. Douchet,
1990 "Steinernema ritteri n.s.
p. (Nematoda: steinernemat
idae) ", Nematologica 36:25.
7-265; Kozodoi, E .; M. 1984 "A Novel Insect Pathogenic Nematode Neoaplectana anomali (Neo)
aplectana anomali) sp. n. (Rh
abditide: steinernematida
e) and its observations on biology ", Zoolog
ical Journal 63: 1605-160
9; Mamiya, Y .; 1988 "Steinernema kushidaii associated with beetle larvae of the scarab beetle from Shizuoka, Japan.
n. sp. (Nematoda: steinernem
atidae) ", Applied Entomolo
gyand Zoology 23: 313-320;
Nguyen, H .; B. And G. C Smart, J
r. 1990 "Stainernema Scapterisi (Ste
inerema scatterisci) n. s
p. (Steinernematidae: Nemat
oda) ”, Journal of Nematolo
gy 22: 187-199; Nguyen, H .; B.
And G. C Smart, Jr. 1992 "Stainerneman neocurtislis
eocurtillis) n. sp. (Rhabdit
ida: steinernematidae) and the key to the species Staynenema ”, Journal o.
f Nematology 24 (print); Poina
r, G. O. , Jr. 1967 "DD-136 nematode (S
tainernematidae: Rhabditoi
dea) and taxonomic position and Neoaplectana Carpocapsae Weiser (Neoplec
Tana carpocapsae Weiser) ”, Proceedings of
the Helminthological Soci
ety of Washington 34: 199-2
09; Poinar, G .; O. 1978 "Strigoderma aboricola (Strigoderma abori
cola) (Fab) Scarab Edae (Scarab)
aeidae) Neoapplectana glastainer (Neoaplectana gla)
seri Steiner) (Steinernema)
tidae: Nematoda): North Ca
in Rolina ", Nematolo
Gica 24: 105-114; Poinar, G .;
O. , Jr. 1979 "Nematodes for biological control of insects" (Nematodes for Biologi)
cal Control of Insects), C
RC Press, Boca Raton; Poina
r, G. O. , Jr. 1985 "South Caro
Lina-derived Neo-Aplectana Intermedia (Ne)
oaplantana intermedia) n. s
p. (Steinernematidae: Nema
toda) ”, Revue de Nematolog
ie 8: 321-327; Poinar, G .; O. ，
Jr. 1988 "Neoaplectana affinis B (Neoaplectana affinis B)
obien) (Rhabditide: stainine
re-description of "rnematidae)", Rev de
Nematologie 1: 143-147; Po
inar, G.M. O. , Jr. 1990 "Steinerne matidae and Heterorhabditide
e) Classification and Biology ", pp. 23-60, R.E. Gau
gler and H.G. K. Edited by Kaya, “Entomomo
pathogenetic Nematodes in Bi
“Logical Control” CRC Pres
S. Boca Raton; Poinar, G .; O. ，
Jr. And E. M. Kozodoi, 1988 "Neoaplanctana
glasseri) and N.N. Anomaly (N. Anoma
li): siblings or similar? , Revue de N
See also ematologie 11: 13-19.

US Pat. No. 4,615,883 discloses insecticidal methods and compositions comprising a hydrated hydrogel capsule carrier containing an insecticidal effective amount of at least one nematode capable of infecting an insect host. The capsule has sufficient hydration power to maintain the viability and infectivity of the nematode. Other carriers or attractants are also known in the art and are described in M.P. Kaib et al., "Trail-followin for termite evidence for multi-component systems."
g) ”, Journal of Chemical E
Cology, JES 8 (1982); Kajiwa.
Ra et al., “Synthetic analogs of termite trailing substances,” A
gric. Biol. Chem. 42 (6), 1293
-1294 (1978).

The present invention provides a hitherto unknown nematode and a biological composition containing it in an amount that effectively kills the non-beneficial worm.

[0006]

SUMMARY OF THE INVENTION In accordance with the above, the present invention provides a termite order, in particular Rhinotermitida.
e) Subtermites, especially R. Flavipes (Rf
lavipes) and R.A. Bibuginix (R. Viv
glycinus) and the like, including a method of controlling pests of the genus Reticulitermes, including species such as G. nicius), which, although not previously named, is referred to herein as the Termite nematode. This includes contacting the worm with an insecticidal amount of an infectious nematode designated as the Termite Nematode, and consequently infecting the worm.

The present invention further provides for the biological control of pests such as the termite Reticulitermes flavipes, which comprises an infectious effective amount of infectious thermite nematode in combination with a carrier and / or attractant. The composition of

The use of this thermite nematode has been found to be particularly effective in killing the termites of the genus Reticulitermes, however, this thermite nematode is also used to kill other pests. It is thought that it can be done. It is also believed that combining the carriers and / or attractants as disclosed herein with thermite nematodes can enhance the effectiveness of the above uses. The invention is described in more detail below.

[0009]

DETAILED DESCRIPTION OF THE INVENTION In this aspect, the claimed invention includes insects, especially reticulate termes, such as R. Bibginix and R.M. Termite nematode, which is a termite parasite such as flavipes n. gen. , N. s
p. (Rhabditide: Steinernematidae)
Is characterized as. The thermite nematode according to the present invention was first discovered in South Florida (South Flori).
da), a termite reticulate termes.
Isolated from Flavipes (Koller). As shown in more detail herein, the nematodes of the claimed invention are considered to be new genera and species. This new genus can be distinguished from the Steinernema genus by the characteristics of female, male and infectious larvae. Females have a prominent lateral caudal gland, a curved tail that is longer than the width of the anus, a larva-bearing female with a spiral shape, and a female that emerges from this female. It can be distinguished from Stainernema females by the pre-infestation larva becoming the infective larva. Males should have a prominent lateral caudal gland, a finger-like tail tip, a characteristic prickle shape (foot shape with a hump on the back), and anterior Having eight reproductive papillae can be distinguished from Stainenema males. Infectious larvae can be distinguished from Stainenema larvae by the presence of a protruding lateral caudal gland and by the presence of a long, curved filamentous tail. Adult nematodes are found outside the termite carcass.

The thermite nematode is a member of the family Stainernematidae, but differs from the genus Stainernema both morphologically and biologically. As will be appreciated from the description herein, the thermite nematodes of the present invention belong to a new genus and new species.

The following materials and methods were used in obtaining the thermite nematode of the claimed invention. The termite, Reticulatermes flavipes (Koller), maintained in several layers of corrugated cardboard, was exposed to infectious larvae (IJ) nematodes. Three to four days later, preterm and adult thermite nematodes emerged from dead termites and became immobile. These adults were collected and used in this description. A filter paper (Whatman) placed on a large piece of cloth placed in a Petri dish (100 x 15 mm).
Infectious larvae (IJ) were collected by placing male and female thermite nematodes and dead termites on top of the number 2, 5.5 mm diameter. 3-4 days later, IJs were collected after they came out of the female. Adults and larvae were examined alive, relaxed, and killed in warm water (40 ° C), fixed in TAF or lactophenol, and then placed in glycerin. . In all cases, coverslip supports were used to avoid flattening the specimen.

These thermite nematodes were prepared for the purpose of performing a scanning electron microscope (SEM) as described below. These thermite nematodes were immobilized in 3% glutaraldehyde buffered to pH 7.2 with 0.1M sodium cacodylate at room temperature (25 ° C) for more than 24 hours. These are used in osmium tetroxide solution.
Post-immobilize at 5 ° C for 12 hours and dehydration in a series of graded ethanol, 50% amyl acetate
Transfer to a mixture of 0% ethyl alcohol for 10 minutes and then transfer into 100% amyl acetate. Then, these thermite nematodes were critical point dried with liquid CO ₂ and placed on SEM stubs before being gold coated. Mating and para-mating were prepared or dissected from specimens in lactophenol, glycerin or 40% lactic acid. Cover glass on glass slide (diameter 12
mm), and after adding one drop of water, the crossing and subcrossing were placed either at the center or near the hair marker.
Excess water was removed using a piece of filter paper. The coverslips were transferred to SEM stubs and sprayed with metallic enamel. One hour later, gold was coated on the crossing and sub-crossing and 15 kv using Hitachi 5-570 SEM.
I inspected it.

A biological test of this thermite nematode was carried out as follows. Each of the thirty-five termites infected with thermite nematode was placed on a double layer filter paper (1 cm ² ) saturated with water. These were dispensed into 4 large (100 x 15 mm) Petri dishes and a few drops of water dropped into each dish to prevent drying.
These termites were observed daily and water was added if necessary. When the termite nematodes emerged from these termites, each unit of filter paper containing termites was individually transferred to a small Petri dish (60 x 15 mm) and water was added to dissect the termites, and The number and life of these thermite nematodes were determined.

The classification of the thermite nematode of the claimed invention can be described as follows. As described herein, the claimed thermite nematode is a stainer nematidae [Chitwood and C
hitwood, 1937, Poinar, 1990]
Belong to. Thermite of the claimed invention
Only one life cycle of nematodes was observed. The following is
A male of the thermite nematode of the claimed invention,
A description of the female and larval stage of infection.

Male : Body: Small (within lactophenol),
The cuticle looks very thick. Mating: A foot with a hump on the back side. Co-Puncture: Almost the same length as a cross-plug (cross-plug /
Vice copulation = 0.97). Reproductive papillae: 13 or 14 pairs, 8 pairs preanal and one single preanal papillae (Figure ID, 2D, FI, 7A). Lateral caudal gland: protruding (Fig. 2H, I). Tail tip: finger-like (figure IF, 2D,
H, I).

Female : Body: Spiral (FIGS. 3A, 4I); usually found in the medium surrounding near infected and dead termites. Tail: Curved longer than the width of the anus (Figs. 3E, GI); the length of the tail divided by the width of the anus is 1.40 (1.10-1.70). .
Lateral caudal gland: Protruding, located at half the tail (Fig. 4E-H). The larva before coming out from this female becomes the infection stage.

Larvae , third infectious period: body: 926 μm long
(789-1,084 μm), and thin (a = 3
9), with a streak. Head: slightly enlarged (Fig. 5
A, 6A). Twin: There is a vertical rift, located below the lip disc (FIGS. 6A, B). Side: 9 notches and 8 smooth bands (Fig. 6F-H). Lateral caudal gland: protruding, cleft-like (Fig. 6E-G), occupying more than half of the lateral sides.
Tail: Same length as esophagus (Table 3), filamentous, curved at the end (Fig. 5B, C; 6D, E).

The scientific classification of the characteristic properties exhibited by the thermite nematode of the claimed invention is as follows. These thermite nematode males have a paw-like appearance of a humpback prickly prickle, the presence of a protruding lateral caudal gland, and the presence of a finger-like tail end. , Having a total of 27-29 reproductive papillae, 8 pairs of them in front of the anus, and the paracoach has the same length as the cobus (in 32% of the males examined, this The collusion was the same as or longer than the copulation), which distinguishes it from a Staynema male. This Termite nematode female has a projecting lateral caudal gland, a curved tail longer than the width of the anus, and a spiral body, and this Distinguished from Stainenema females by the emergence of infected larvae from dead females. The female is usually found outside the termite carcass. The infectious larvae of thermite nematode have a thin and elongated body (a = 39), which is 10 times the body width of the anus (range 8-11 times), and is almost the same length as the esophagus. It is distinguished from Stainenema larvae by having a ventral curved filamentous tail with and by having a projecting lateral caudal gland.

Thermite nematode species as shown in FIGS. 1-8 are described below with reference to a positive control sample (male in glycerin).

Males : The measurements for 25 males are reported in Table 1. No second generation males were found. This male was smaller than the female. The body was C-shaped, curved ventral when killed by heat. There was a side with a notch that would sometimes be indistinguishable. The mouth is shallow, cheiro lab Zion (cheilo)
rhabdions) and other parts of the mouth form a funnel-shaped structure, its wall, especially the dorsal side, is thickened in front of it and its front end is raised, lateral Viewed from above, it forms a subtriangular ring with sharp tips likened to one dorsal and one ventral tooth (FIG. 1C). The esophagus is Steiner nematoid (stei)
nernematoid (typical of the Stainene nematidae), but was somewhat thinner and extended longer than that of Stainenema. The esophagus-enteral valve was prominent. The drainage hole was located in the middle of the esophagus or in front of it, and the front of the drainage tube was hardened. The gonads were single testicles and warped. The crossing piercing is paired, its shape is paw-like, there is a protruding hump on the back side of the crossing piercing, the front of the head of the crossing piercing is large, and the tip of the crossing piercing Is pointed and slightly curved ventrally, with two protruding sticks present in each interstitial prick (FIGS. 1E-H; 2A, C; 8B, D).
The length of co-plunge (collateral pricking / cross-plunging ratio is 0.97), 2
Eight out of five males had a sub-stab equal to or longer than the stab (Figs. 8C, D), and the base end of this sub-stab is normally viewed in situ. Was difficult (Figs. 1F-H), but visible after they were dissected and could be accurately measured (Figs. 2A, B; 8C, D). The drainage area formed a long tube around the cross connect (Fig. 2D, F). Reproductive papillae: 13 to 14 pairs, with a single pre-excretory papillae on the ventral side (Fig. 2D-I); 8 pairs are preanal and mostly subventral (subventral) , With some variation in their positions (FIG. 2D; 7A), and one pair in the stab area (FIG. 2F), three pairs caudally near the abdomen, and 2 A pair exists on the caudal side close to the back (Fig. F, 2G, H, I). The lateral caudal gland was protruding (Fig. 2
H, I). The tail is a conical body, and the tip of the tail is finger-shaped (Fig. 2).
I), but varied, especially in young males.

Females : The measurements for 25 females are reported in Table 2. There were no second generation females in this termite. Females were much larger than males. The mature female body is helical (Fig. 3A; 4
1) was usually found in the medium surrounding near infected and dead termites. The cuticle was ring-shaped. No flanks were observed. Head: round or truncated (Fig. 3F), or sometimes larger, especially in young females (Fig. 4D). Six lip nipples were protruding and very often there were numerous additional nipple-like structures present with the nipple (Fig. 4A, B, D). The mouth is essentially the same as the male, but with all structures larger and more prominent, seen from the side (Figs. 3B, F; 4A,
B), a raised tube or tooth-like structure was protruding in the mouth. Esophageal: Steiner nematice
typical of nematids (Fig. 3F), the body corporus is tubular and muscular, the metacorpus of the body is slightly swollen nonvalvate, and the isthmus is present. But not protruding, and the basal sphere (basal
bulb) was muscular, enlarged and valve-opened. A nerve ring surrounded the isthmus. The esophagus-intestinal valve was protruding (Fig. 3F). There was a vent hole in the middle or in front of the esophagus. The discharge tube was large and the front part was fully hardened. The gonads were twin wombs, both wombs, and warped. The vulva: A lateral rift that is not protruding in females with eggs in the body, but slightly protruding in females with larvae in the body. The epiptygma was absent. Vagina: It was hard. Tail: Conical body, curved, longer than the width of the body in the anus (Figs. 3G-I; 4E-).
H), the value obtained by dividing the length of the tail by the width of the body of the anus is 1.40.
It was (1.10-1.70). Lateral caudal gland: protruding, located in the posterior half of the tail (Fig. 4E-H).

Third Stage of Larval Infection: The measurements for 50 third stage infectious larvae (IJ) are reported in Table 3. Larvae become infectious larvae before they emerge from the female. Body: It was thin and elongated, and had sufficient muscle (Figs. 6A and 6C). Shell (second keratin): Present but sometimes lost.
Head region: smooth and mostly slightly larger (FIGS. 5A, 6A). Mouth opening: closed and extending in three directions (FIG. 6C), with six lips (FIGS. 6A-C).
Lip papillae: not observed, four nipple papillae protruding and located well behind the anterior end (Figs. 6A-C). Twin: Large cleft-like position, posterior to the lips, but anterior to the papilla (FIGS. 6A-C). Esophagus: It was degenerated. Basal sphere: It had a valve and was long.
Cardia: Present but sometimes unclear. Discharge hole:
It was sticking out. Discharge tube: Long and wavy as clearly seen in living specimens. The stiffening structure associated with the drainage tube immediately in front of the draining cells is viewed laterally (FIG. 5A,
D) Seen as two black dots. Lateral: In the middle part of the body, wider than 1/3 of the width of the body, usually only two limbs are clearly visible (Fig. 6I), with 8 smooth bands in the anterior and posterior regions, There are 9 protruding notches (Fig. 6F-H). The tail is 1 of the width of the anus
It extends to 0 times (8-11 times), and its posterior end is always curved to the ventral side. Average tail length: The same as the length of the esophagus. Lateral caudal gland: protruding and cleft-like (Fig. 6F, G). Left and right lateral caudal glands: not at the same level (FIG. 5B; 8A) but usually located 25 to 50 μm posterior to the anus and occupy 3 or 4 lateral girdles.

The biology of this thermite nematode can be described as follows. This thermite nematode developed only in the termite head. When the termite tissue began to collapse, the adult emerged in the medium surrounding it. The number of adults within each termite varied from 1 to 4 (1 was 26% and 2 were 4).
0%, 3 20% and 4 14%
Met). Of the 35 termites, 20 had both males and females, and 15 had either males or females. No reproduction occurred at all if only one sex was present. In the presence of both males and females, after mating, the females shed slightly from their dead termites, becoming spiraled (Fig. 7B), immobile, producing eggs and hatching in the body. Occurred. Before the larva emerged from the female, it developed and became infectious inside the female. Females simultaneously produced the first, second and infested larvae. When most of these larvae reach their infectious stage, they move violently, destroying either the tail region or the mouth region of the female's body in the medium surrounding it. Moved to. A large number of bacterial cells were observed in the female body while the larvae were growing.

In most Stayne nematides,
Adults emerge from the carcass of the insect only when it is present in excess in the insect. The thermite nematode adult of the present invention usually emerges from its host carcass and into the medium surrounding it. Without being limited in scope to any particular theory, this is
The termites are small in size and are likely to easily become too dense. Host range studies will help determine if such anomalous properties occur in all hosts. If this trait persisted, it would be an important biological trait for this novel species or perhaps genus. Another significant biological trait is that these eggs hatch in their female bodies to become infectious larvae. While not limiting in scope, it is believed that such traits may be associated with the egress and departure of females from the corpse of this host. If the female were to lay its eggs in its environment, these larvae would probably have no bacterial source from which they were preyed to grow. Inside the female's body, the larva is thought to feed on bacteria that enter the body after the female dies. Morphologically, the male peculiar shape of the interlacing (foot with a hump on the surface of the back), the caudal end of the finger, and the 8 pairs of preanal reproductive papillae are known to Stainene nematides. It is new and unique to this new genus. For the female, its tail was longer than the width of its anal body, and the presence of a protruding lateral caudal gland in first-generation females was not reported in Stainernema. These traits are also important features for the identification of this new genus. In infectious larvae, the filamentous, curved tail and protruding lateral cochlear glands are the distinguishing features of this novel genus. Since some of the above mentioned traits are new to the family Stanelnematidae, their scientific classification needs to be corrected. As will be appreciated, the family of true parasites Stainene nematidae [Chitwood &
Chitwood 1937] has symbiotic bacteria in its digestive system. Both males and females are required for breeding.

[0025]

[Table 1]

[0026]

[Table 2]

[0027]

[Table 3] As set forth herein, embodiments of the present invention as a composition for biologically controlling termite pests include the use of a carrier and / or attractant to kill infectious thermite nematode. Included in effective amount. This carrier may be selected from the group consisting of oils, waxes, hydrogels, water thickeners and other gelling agents. This attractant is 4-phenyl-3-buten-1-ol or an analogue thereof, a natural termite trailing pheromone containing higher alcohols such as (Z, Z, E) -3,6,8-dodecatriene. -1-ol or synthetic analogues such as (Z, Z) -3,6-dodecadien-1-ol (V),
(Z) -3-dodecen-1-ol, (Z, Z) -3,5
-Dodecadien-1-ol, (Z, E) -3,5-dodecadien-1-ol and (Z, Z, Z) -3,6,8-
Dodecatriene-1-ol, (Z, Z) -3,6- diene-1-ol isomer _{(C 8 -C 12), (} Z, Z) -3,
6-octadiene-1-ol (I), (Z, Z) -3,
6-nonadiene-1-ol (II), (Z, Z) -3,
6-decadiene-1-ol (III), (Z, Z)-
It may be selected from the group consisting of 3,6-undecadiene-1-ol (IV) and (Z, Z) -3,6-dodecadien-1-ol (V).

Thermite nematodes according to the present invention showed termite mortality as shown below.

Infectivity test 1 (VBL-92-4862) shown by entomopathogenic nematodes against termites Termite mortality (Abbott) (%) 7 days after treatment 0 (worm) 72 termites: reticle Termes virginix nematode: (new thermite nematode) Test unit: Put 20 grams of sterile Florida sand soil in a Petri dish, and place one 12 mm diameter filter paper plate on it.

Infestation: 50 termites per petri dish Replication: 4 Nematode inoculation: 4600 insects per petri dish Infectivity test 2 (VBL) shown by entomopathogenic nematodes to termites -92-4786) Termite mortality 9 days after treatment Number of nematodes recovered (%) from dead termites Control (none) 1.3 0 nematodes 77.3 51.7 Termites: Reticulitermes flavipes Other Test conditions: Same as test 1.

Although the present invention has been described above in detail for purposes of illustration, the above details are for that purpose only, and are intended to be within the spirit and scope of the invention, except as may be limited by the following claims. It should be understood that modifications without departing can be made by those skilled in the art.

[Brief description of drawings]

FIG. 1: Thermite Nematode n. g. n. sp. 2 is a schematic view showing a male morphology. A) Whole body. B) Forward area. C) Expansion of the anterior end. D) The posterior area showing genital papillae, copulation and paracopulation. E) Cross- and sub-crossing (one on the ventral side and one close to the side). F) Normal tail. F-K) Deformation of tail shape.

FIG. 2: Thermite nematode n. g. n. sp. 8 is an SEM photograph replacing an enlarged view of a fine portion of FIG. A) Crossing and side-plugging. B) shows the end of a sub-crossing (arrow), the end of another crossing, and another side of the sub-lapping,
Enlargement of the crossing tip. C) Two crossing sticks showing a hump on the back (arrow). D) Posterior area showing the genital papilla, drainage duct, protruding stab stick and finger tail end. E)
Enlargement of the most anterior reproductive papilla. F) Enlargement of a single (s) ventral reproductive papilla and part of the male tail showing each one of pairs 8 and 9. G) Reproductive papilla, pair 10, 11 and 1
Each one of 2. H) Reproductive papillae, pair 13 and pair 1 of 14
One, the lateral caudal gland (p), and the end of the digital tail. I) reproductive teats,
One of pair 13, the caudal gland (p), and the tail of the digital tail. All magnifications are based on the scale bar in A. A = 2
3 μm, B = 2.5 μm, C = 20 μm, D = 43 μm
m, E = 2.3 μm, F = 12 μm, G = 10 μm, H
= 6 μm, I = 5 μm.

FIG. 3: Thermite nematode n. g. n. sp. 2 is a schematic view showing the morphology of the female of FIG. A) Whole body. B) Front end. C) A protruding vulva of a female with larvae in the body.
D) A vulva that does not have a female larva in its body and does not stick out. E, G, H, I) Deformation of tail shape. F) Forward area.

FIG. 4 Thermite nematode n. g. n. sp. 3 is an SEM photograph replacing an enlarged view of a fine portion of the female of FIG. A-
C) Facial view: some papillary structures near the papillae on facial views A and B, and raised three-way openings of the mouth in A and B. D) Anterior area of young female showing separated lip area, papillae (I), papillae (c) and twin (a). These papillae-like structures are located near the lip papillae. EG) Caudal deformity showing protruding lateral caudal glands (p). H) Enlargement of the lateral caudal gland showing a raised rounded area with a clear opening in the center. I) Whole body. All magnifications are based on the scale bar in A. A = 12 μm, B, C = 7.5 μ
m, D = 10 μm, E = 13.6 μm, F = 30 μm,
G = 17.6 μm, H = 7.5 μm, I = 475 μm.

FIG. 5: Thermite nematode n. g. n. sp. FIG. 3 is a partially enlarged view of a third stage infectious larva of the above. A) Anterior region showing long wavy drainage tubes terminating within the draining cells.
B) Vental view showing tail, two protruding lateral caudal glands.
C) A posterior region with a long curved tail. D) Enlargement of the esophageal portion showing a structure in which the entrance to the draining cells is stiffened.

FIG. 6: Thermite nematode n. g. n. sp. 3 is an SEM photograph replacing an enlarged view of a fine part of the infectious larva of the above. A) Anterior region showing smooth, swollen head, twin (a) and nipple (c). B) Enlargement of the anterior end showing 6 lips, mouth opening, twins and papillae.
C) Anterior region of infectious larva with shells showing twins, three-way mouth opening (m) and protruding papilla (c). D) A posterior region with a ventral curved filamentous tail. E) A tail with a large lateral caudal gland and a hooked end. F) Lateral side with 8 lateral bands (9 notches) and large lateral caudal gland. G) Lateral and large lateral caudal glands. H) Side with 8 bands. I) Mid-body side, with two rims and an obscured inner band. All magnifications are based on the scale bar in A. A = 3 μm, B = 1.8 μm,
C = 2.5 μm, D = 43 μm, E = 17 μm, F = 4.
3 μm, G = 1.8 μm, H = 5 μm, I = 10 μm.

FIG. 7: Thermite nematode n. g. n. sp. 8 is an SEM photograph replacing an enlarged view of a fine portion of FIG. A) Posterior region of the male showing 8 preanal reproductive papillae. B) A female coming out of a dead termite (upper right).

FIG. 8: Thermite nematode n. g. n. sp. An optical micrograph that replaces a partially enlarged view of. A) Infectious larva (ventral) tail with two protruding lateral caudal glands. B) A crossing puncture showing a foot-like form with a hump on the back when viewed from the side. C) Subcolic puncture (view close to the ventral side). D) Cross-connect (viewed from the side) shows that this cross-insert is shorter than the sub-insert of C. C and D were from the same male.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Bruce J. A. Monk, Florida, United States 32962 Bellovich Thirty Austerasu 625 (72) Inventor Walter M. Zetz, Florida 32963 Bellovich, Pebble Bay South 4645

Claims (6)

[Claims] 1. A method for controlling a pest, comprising the thermite nematode (Term) in an infectious period so that the insect is infected.
A method comprising contacting an insecticidal amount of ete Nematodes) with the insect. 2. The method according to claim 1, wherein the pest is of the order Termite. 3. The insect is R. Flavipes and R.M. The method of claim 1, wherein the termite is of the genus Reticulitermes selected from the group consisting of Bibginix. 4. A composition for biologically controlling termite pests comprising an insecticidal effective amount of infectious thermite nematodes and a carrier and / or attractant. 5. A composition according to claim 4, wherein said carrier is selected from the group consisting of oils, waxes, hydrogels, water thickeners and other gelling agents. 6. The composition according to claim 5, wherein the attractant is a natural or synthetic termite trail pheromone.