JPH0764698B2 - Soil pest control material - Google Patents
Soil pest control materialInfo
- Publication number
- JPH0764698B2 JPH0764698B2 JP5211111A JP21111193A JPH0764698B2 JP H0764698 B2 JPH0764698 B2 JP H0764698B2 JP 5211111 A JP5211111 A JP 5211111A JP 21111193 A JP21111193 A JP 21111193A JP H0764698 B2 JPH0764698 B2 JP H0764698B2
- Authority
- JP
- Japan
- Prior art keywords
- soil
- bacterium
- pest control
- sclerosis
- insect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000002689 soil Substances 0.000 title claims description 47
- 239000000463 material Substances 0.000 title claims description 36
- 241000607479 Yersinia pestis Species 0.000 title claims description 21
- 241000894006 Bacteria Species 0.000 claims description 43
- 241000233866 Fungi Species 0.000 claims description 22
- 241000238631 Hexapoda Species 0.000 claims description 22
- 208000034189 Sclerosis Diseases 0.000 claims description 22
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 claims description 9
- 244000005700 microbiome Species 0.000 claims description 8
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 7
- 239000011707 mineral Substances 0.000 claims description 7
- 239000010451 perlite Substances 0.000 claims description 6
- 235000019362 perlite Nutrition 0.000 claims description 6
- 241000186361 Actinobacteria <class> Species 0.000 claims description 5
- 229910021536 Zeolite Inorganic materials 0.000 claims description 4
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 239000010457 zeolite Substances 0.000 claims description 4
- 241000223679 Beauveria Species 0.000 claims description 3
- 239000005416 organic matter Substances 0.000 claims description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 19
- 241000209094 Oryza Species 0.000 description 9
- 235000007164 Oryza sativa Nutrition 0.000 description 9
- 235000009566 rice Nutrition 0.000 description 9
- 240000006439 Aspergillus oryzae Species 0.000 description 6
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 241001446247 uncultured actinomycete Species 0.000 description 6
- 239000000203 mixture Substances 0.000 description 4
- 241000222178 Candida tropicalis Species 0.000 description 3
- 241000187747 Streptomyces Species 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241000751139 Beauveria bassiana Species 0.000 description 2
- 241000223201 Metarhizium Species 0.000 description 2
- 241000228143 Penicillium Species 0.000 description 2
- 241000191025 Rhodobacter Species 0.000 description 2
- 241000365764 Scirtothrips dorsalis Species 0.000 description 2
- 241000187392 Streptomyces griseus Species 0.000 description 2
- 241001414989 Thysanoptera Species 0.000 description 2
- 241001463008 Berberia Species 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 241000188153 Isaria fumosorosea Species 0.000 description 1
- 239000005908 Isaria fumosorosea Apopka strain 97 (formely Paecilomyces fumosoroseus) Substances 0.000 description 1
- 101100412856 Mus musculus Rhod gene Proteins 0.000 description 1
- 241000130993 Scarabaeus <genus> Species 0.000 description 1
- 101150094640 Siae gene Proteins 0.000 description 1
- 241000255588 Tephritidae Species 0.000 description 1
- 101100242191 Tetraodon nigroviridis rho gene Proteins 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 241000339373 Thrips palmi Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 241000223260 Trichoderma harzianum Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000459 effect on growth Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、昆虫硬化病菌を含む土
壌害虫抑制資材に関する。更に詳しくは昆虫硬化病菌が
土壌中で長期に安定化された昆虫硬化病菌を含む土壌害
虫抑制資材に関する。TECHNICAL FIELD The present invention relates to a soil pest control material containing insect sclerosis bacterium. More specifically, it relates to a soil pest control material containing an insect sclerosis bacterium which is stabilized in the soil for a long period of time.
【０００２】[0002]
【従来の技術】多くの害虫の生活サイクルを見てみると
卵→幼虫→蛹→成虫が地上で行われているが、甲虫類、
マメハモグリバエやウジバエのようなハエの一部、チャ
ノキイロアザミウマやミカンキイロアザミウマのような
スリップス類には幼虫や蛹の時代を土中で過ごすことが
知られている。2. Description of the Related Art Looking at the life cycle of many pests, eggs → larvae → pupae → adults are performed on the ground, but beetles,
It is known that some larvae such as the leaf fly and the fruit fly, and thrips such as the flower thrips and the flower thrips, spend the period of larvae and pupae in the soil.
【０００３】一方、これら土壌害虫の防除技術としては
従来から農薬による土壌消毒が主流をなしているが、こ
れらの方法では益虫、有用微生物も一網打尽にし、この
ことにより土壌生態系が破壊され作物の生育に少なから
ず悪影響を及ぼしている。このため、生態系を守るため
天敵微生物、例えば昆虫硬化病菌を用いた土壌害虫防除
も試みられているが、土壌中での環境要因に左右されて
長期間定着が難しいためごく限られた場所で実施されて
いるのが現状である。On the other hand, as a technique for controlling these soil pests, soil disinfection with pesticides has been the mainstream for a long time, but these methods also exhaust beneficial insects and useful microorganisms to one net, which destroys the soil ecosystem and causes crop damage. It has a considerable negative effect on growth. For this reason, it has been attempted to control soil pests using natural enemy microorganisms such as insect sclerosis fungi to protect the ecosystem, but it is difficult to settle for a long period of time due to environmental factors in the soil, so it is limited in very limited places. It is currently being implemented.
【０００４】[0004]
【発明が解決しようとする課題】本発明の目的は、昆虫
硬化病菌を土壌中で安定化させた昆虫硬化病菌を含む土
壌害虫抑制資材を提供することにある。SUMMARY OF THE INVENTION An object of the present invention is to provide a soil pest control material containing an insect sclerosis bacterium which is stabilized in soil.
【０００５】[0005]
【課題を解決するための手段】すなわち、本発明は細
菌、酵母菌、放線菌及び糸状菌から選ぶ微生物の１種又
は２種以上と昆虫硬化病菌とを植物性有機物にて発酵し
て得られる発酵物及び天然鉱物若しくはその加工品を含
むことを特徴とする土壌害虫抑制資材である。上記昆虫
硬化病菌としては、ボーベリア（Ｂｅａｕｖｅｒｉａ）
属、メタリジュウム（Ｍｅｔａｒｈｉｚｉｕｍ）属又は
パエシロミセス（Ｐａｅｃｉｌｏｍｙｃｅｓ）属に属す
る微生物が挙げられる。また、天然鉱物としては、パー
ライト、ゼオライト又はパーミキュライトが挙げられ
る。That is, the present invention can be obtained by fermenting one or more kinds of microorganisms selected from bacteria, yeasts, actinomycetes and filamentous fungi and insect sclerosis fungi with plant organic matter. A soil pest control material comprising a fermented product and a natural mineral or a processed product thereof. As the above-mentioned insect sclerosis bacterium, Beauveria
Examples thereof include microorganisms belonging to the genus, Metarhizium genus, or Paecilomyces genus. In addition, examples of natural minerals include perlite, zeolite, and permiculite.
【０００６】さらに、本発明は上記の土壌害虫抑制資材
を土壌に施用することを特徴とする土壌害虫抑制方法で
ある。以下、本発明を詳細に説明する。本発明に用いる
微生物としては、昆虫硬化病菌、細菌、酵母菌、放線
菌、糸状菌であれば特に制限はなくいずれもが使用する
ことができる。Furthermore, the present invention is a method for controlling soil pests, which comprises applying the above-mentioned soil pest control material to soil. Hereinafter, the present invention will be described in detail. The microorganism used in the present invention is not particularly limited as long as it is an insect sclerosis bacterium, a bacterium, a yeast, an actinomycete, or a filamentous fungus, and any microorganism can be used.
【０００７】昆虫硬化病菌としては、ボーベリア・バシ
アナ（Ｂｅａｕｖｅｒｉａ ｂａｓｓｉａｎａ）（ＩＦ
Ｏ ３１６７６）、メタリジュウム・アニソプリアエ
（Ｍｅｔａｒｈｉｚｉｕｍ ａｎｉｓｏｐｌｉａｅ）
（ＩＦＯ ３１６８２），パエシロミセス・フムソロセ
ウス（Ｐａｅｃｉｌｏｍｙｃｅｓ ｆｕｍｏｓｏｒｏｓ
ｅｕｓ）（ＩＦＯ ７０７２）が挙げられる。。細菌と
しては、バチルス・サブチルス（Ｂａｃｉｌｌｕｓ ｓ
ｕｂｔｉｌｉｓ）（ＩＦＯ ３３３６）、バチルス・セ
レウス（Ｂａｃｉｌｌｕｓ ｃｅｒｅｕｓ）（ＩＦＯ
３４６６）、ロドバクター・スファロイデス（Ｒｈｏｄ
ｏｂａｃｔｅｒ ｓｐｈａｅｒｏｉｄｅｓ）（ＩＦＯ
１２２０３）等が、酵母菌としてはサッカロミセス・セ
レビシエ（Ｓａｃｃｈａｒｏｍｙｃｅｓ ｃｅｒｅｖｉ
ｓｉａｅ）（ＩＦＯ ２３４２），カンディダ・トロピ
カリス（Ｃａｎｄｉｄａｔｒｏｐｉｃａｌｉｓ）（ＩＦ
Ｏ ０６１８）等が、放線菌としては、ストレプトミセ
ス・グリセウス（Ｓｔｒｅｐｔｏｍｙｃｅｓ ｇｒｉｓ
ｅｕｓ）（ＩＦＯ３４３０）、ストレプトミセス・フラ
ジエ（Ｓｔｒｅｐｔｏｍｙｃｅｓ ｆｒａｄｉａｅ）
（ＩＦＯ ３４３９）等が、糸状菌としては、アスペル
ギルス・オリザエ（Ａｓｐｅｒｇｉｌｌｕｓ ｏｒｙｚ
ａｅ）（ＩＦＯ ５２３９）、ペニシリウム・アイスラ
ンディカム（Ｐｅｎｉｃｉｌｌｉｕｍ ｉｓｌａｎｄｉ
ｃｕｍ）（ＩＦＯ ６９６３）、トリコデルマ・ハルジ
アナム（Ｔｒｉｃｈｏｄｅｒｍａ ｈａｒｚｉａｎｕ
ｍ）（ＩＦＯ ３１９７６）等がそれぞれ挙げられる。[0007] Examples of insect sclerosis fungi include Beauveria bassiana (IF
O 31676), Metalrhizium anisopriae
(IFO 31682), Paecilomyces fumosoros
eus) (IFO 7072). . Bacteria include Bacillus subtilis
ubtilis (IFO 3336), Bacillus cereus (IFO)
3466), Rhodobacter Sphaloides (Rhod)
object sphaeroides) (IFO
12203) and the like as Saccharomyces cerevisiae (Saccharomyces cerevisiae).
siae) (IFO 2342), Candida tropicalis (IF
O 0618) and the like are Streptomyces griseus (Streptomyces gris) as actinomycetes.
eus) (IFO 3430), Streptomyces fraddiae
(IFO 3439) and the like, Aspergillus oryzae (Aspergillus oryzae (Aspergillus oryzae)
ae) (IFO 5239), Penicillium islandic
cum) (IFO 6963), Trichoderma harzianu
m) (IFO 31976) and the like.
【０００８】本発明において、これらの昆虫硬化病菌、
細菌、酵母菌、放線菌及び糸状菌の混合比率は、適宜な
比率が用いられるが、好ましくは５：１：２：１：１で
ある。本発明に用いる天然鉱物は特に限定されず、通常
土壌改良資材として使用されるパーライト、ゼオライト
又はパーミキュライトが用いられる。In the present invention, these insect sclerosis fungi,
The mixing ratio of bacteria, yeasts, actinomycetes and filamentous fungi may be any suitable ratio, but is preferably 5: 1: 2: 1: 1. The natural mineral used in the present invention is not particularly limited, and perlite, zeolite or permiculite which is usually used as a soil improving material is used.
【０００９】本発明において、発酵物と天然鉱物との配
合割合は、重量比で、好ましくは９９：１〜１：９９、
さらに好ましくは２：８〜５：５である。本発明の土壌
害虫抑制資材は、土壌に施用することにより昆虫硬化病
菌が土壌中で長期間定着し、土壌害虫を効果的に抑制す
ることができる。一般的に土壌害虫が硬化病菌に感染す
るには土壌中の分生子数が土壌１ｇあたり１×１０５個
〜１×１０６個必要であると言われている。したがっ
て、本発明の土壌害虫抑制資材の土壌への散布量は、１
００ｍ２当たり２．５〜３．５ｋｇである。In the present invention, the mixing ratio of the fermented product and the natural mineral is preferably 99: 1 to 1:99 by weight.
More preferably, it is 2: 8 to 5: 5. When the soil pest control material of the present invention is applied to the soil, the insect sclerosis disease bacteria settle in the soil for a long period of time, and the soil pests can be effectively suppressed. It is generally said that the number of conidia in the soil is required to be 1 × 10 5 to 1 × 10 6 per 1 g of soil in order for soil pests to be infected with sclerosis bacterium. Therefore, the amount of the soil pest control material of the present invention applied to the soil is 1
It is 2.5 to 3.5 kg per 00 m 2 .
【００１０】本発明において抑制対象となる土壌害虫と
しては、チャノキイロアザミウマ、マメハモグリバエ、
コガネムシ等が挙げられる。Soil pests to be controlled in the present invention include tea thrips, Thrips palmi L.,
Examples include scarabs.
【００１１】[0011]
【発明の効果】この土壌害虫抑制資材は、土壌に施用さ
れたとき昆虫硬化病菌の分生子数が長期間安定に保持さ
れるEFFECTS OF THE INVENTION This soil pest control material, when applied to soil, stably maintains the number of conidia of insect sclerosis disease bacteria for a long period of time.
【００１２】[0012]
昆虫硬化病菌としてのメタリジュウム・アニソプリアエ
（ＩＦＯ ３１６８２）（メタリジュウム菌）、酵母菌
としてのサッカロミセス・セレビシエ（ＩＦＯ２３４
２）、細菌としてのバチルス・サブチルス（ＩＦＯ ３
３３６），糸状菌としてのアスペルギルス・オリザエ
（ＩＦＯ ５２３９）をそれぞれ米、米ヌカ、フスマで
培養し菌濃度が１０９ｃｆｕ／資材１ｇ（メタリジュウ
ム菌），１０８ｃｆｕ／資材１ｇ（酵母菌），１０９ｃ
ｆｕ／資材１ｇ（バチルス菌），１０８ｃｆｕ／資材１
ｇ（糸状菌）の培養物を得た。これらを重量比でメタリ
ジュウム菌３：酵母菌１：バチルス菌１：糸状菌１：パ
ーライト４の割合で混合しその混合物１００ｇを３．３
ｍ２の土壌に混和しメタリジュウム菌の土壌定着度合い
を試験した。同じように対照区には米に培養したメタリ
ジュウム菌（菌密度１０９ｃｆｕ／資材１ｇ）１００ｇ
を用いた。この両者について、土壌１ｇ当たりのメタリ
ジュウム菌（昆虫硬化病菌）生息数を調べた。結果を表
１に示す。Metallisium anisopriae (IFO 31682) (metalridium) as an insect sclerosis bacterium, Saccharomyces cerevisiae (IFO234) as a yeast
2), Bacillus subtilis as a bacterium (IFO 3
336), Aspergillus oryzae (IFO 5239) as a filamentous fungus is cultivated in rice, rice bran, and bran respectively to have a bacterium concentration of 10 9 cfu / g of material (metallidium bacterium), 10 8 cfu / g of material (yeast) , 10 9 c
fu / Material 1 g (bacillus), 10 8 cfu / Material 1
A culture of g (filamentous fungus) was obtained. These were mixed in a weight ratio of metallidium bacterium 3: yeast 1: bacillus 1: filamentous fungus 1: perlite 4 and 100 g of the mixture was mixed with 3.3.
The mixture was mixed with m 2 soil to test the degree of soil colonization of Metaridium. Similarly, in the control area, 100 g of metallidium bacterium cultured on rice (bacterial density 10 9 cfu / 1 g of material)
Was used. With respect to both of these, the number of metarhizium bacteria (insect sclerosis bacterium) per 1 g of soil was examined. The results are shown in Table 1.
【００１３】[0013]
【表１】 [Table 1]
【００１４】〔実施例２〕 昆虫硬化病菌としてのパエシロミセス・フムソロセウス
（ＩＦＯ ７０７２）（パエシロミセス菌）、酵母菌と
してのカンディダ・トロピカリス（ＩＦＯ ０６１
８）、放線菌としてのストレプトミセス・グリセウス
（ＩＦＯ ３４３０）、糸状菌としてのペニシリウム・
アイスランディカム（ＩＦＯ ６９６３）をそれぞれ
米、米ヌカ、フスマで培養し菌密度が１０９ｃｆｕ／資
材１ｇ（パエシロミセス菌），１０８ｃｆｕ／資材１ｇ
（酵母菌），１０６ｃｆｕ／資材１ｇ（放線菌），１０
８ｃｆｕ／資材１ｇ（糸状菌）の培養物を得た。これら
を重量比でパエシロミセス菌３：酵母菌１：放線菌１：
パーライト４の割合で混合しその混合物１００ｇを３．
３ｍ２の土壌に混和しパエシロミセス菌の土壌定着度合
いを試験した。[Example 2] Paecilomyces humrosortheus (IFO 7072) (Paecilomyces bacterium) as an insect sclerosis bacterium, and Candida tropicalis (IFO 061) as a yeast.
8), Streptomyces griseus as actinomycete (IFO 3430), Penicillium as filamentous fungus
Icelandicam (IFO 6963) was cultivated in rice, rice bran, and bran respectively, and the bacterial density was 10 9 cfu / g 1 material (Paecilomyces bacterium), 10 8 cfu / g 1 material
(Yeast), 10 6 cfu / g of material (actinomycete), 10
A culture of 8 cfu / g of material (filamentous fungi) was obtained. These are in a weight ratio of Paecilomyces bacterium 3: yeast 1: actinomycete 1:
Perlite 4 was mixed and 100 g of the mixture was mixed with 3.
It was mixed with 3 m 2 of soil to test the degree of soil colonization of Paecilomyces bacterium.
【００１５】対照区にはフスマに培養したパエシロミセ
ス菌（菌密度１０９ｃｆｕ／資材１ｇ）１００ｇを用い
た。この両者について、土壌１ｇ当たりのパエシロミセ
ス菌（昆虫硬化病菌）生息数を調べた。結果を表２に示
す。As a control, 100 g of Paecilomyces bacterium (bacterial density: 10 9 cfu / 1 g of material) cultured in bran was used. For both of them, the number of Paecilomyces bacterium (insect sclerosis disease) inhabited per 1 g of soil was examined. The results are shown in Table 2.
【００１６】[0016]
【表２】 [Table 2]
【００１７】〔実施例３〕 昆虫硬化病菌としてのボーベリア・バシアナ（ＩＦＯ
３１６７６）（ボーベリア菌），細菌としてのバチルス
・セレウス（ＩＦＯ ３４６６），細菌としてのロドバ
クター・スファロイデス（ＩＦＯ １２２０３），糸状
菌としてのアスペルギルス・オリザエ（ＩＦＯ ５２３
９），糸状菌としてのトリコデルマ・ハルジアナム（Ｉ
ＦＯ ３１９７６）をそれぞれ米、米ヌカ、フスマで培
養し菌密度が１０９ｃｆｕ／資材１ｇ（ボーベリア
菌），１０８ｃｆｕ／資材１ｇ（細菌），１０６ｃｆｕ
／資材１ｇ（細菌），１０８ｃｆｕ／資材１ｇ（糸状
菌），１０７ｃｆｕ／資材１ｇ（糸状菌）の培養物を得
た。これらを重量比でボーベリア菌３：細菌１：細菌
０．５：糸状菌１：糸状菌０．５：ゼオライト４の割合
で混合しその混合物１００ｇを３．３ｍ２の土壌に混和
しボーベリア菌の土壌定着度合いを試験した。対照区に
はフスマに培養したボーベリア菌（菌密度１０９ｃｆｕ
／資材１ｇ）１００ｇを用いた。Example 3 Beauveria bassiana (IFO) as an insect sclerosis bacterium
31676) (Boberia), Bacillus cereus as a bacterium (IFO 3466), Rhodobacter sphaloides (IFO 12203) as a bacterium, Aspergillus oryzae (IFO 523) as a filamentous fungus.
9), Trichoderma harzianum as a filamentous fungus (I
FO 31976) was respectively cultivated in rice, rice bran, and bran, and the bacterial density was 10 9 cfu / material 1 g (Boberia bacterium), 10 8 cfu / material 1 g (bacteria), 10 6 cfu.
A culture of 1 g of material (bacteria), 10 8 cfu / g of material (filamentous fungus), 10 7 cfu / g of material (filamentous fungus) was obtained. These were mixed in a weight ratio of berberia 3: bacteria 1: bacterium 0.5: filamentous fungus 1: filamentous fungus 0.5: zeolite 4 and 100 g of the mixture was mixed with 3.3 m 2 of soil to prepare The degree of soil colonization was tested. In the control area, a Buberia bacterium (cultivation density 10 9 cfu
/ 1 g of material) 100 g was used.
【００１８】この両者について、土壌１ｇ当たりのボー
ベリア菌（昆虫硬化病菌）生息数を調べた。結果を表３
に示す。With respect to both of these, the number of Beauveria (insect sclerosis) inhabited per 1 g of soil was examined. The results are shown in Table 3.
Shown in.
【００１９】[0019]
【表３】 [Table 3]
【００２０】〔実施例４〕 昆虫硬化病菌としてのメタリジュウム・アニソプリアエ
（ＩＦＯ ３１６８２）（メタリジュウム菌）、酵母菌
としてのサッカロミセス・セレビシエ（ＩＦＯ２３４
２）、酵母菌としてのカンディダ・トロピカリス（ＩＦ
Ｏ ０６１８），放線菌としてのストレプトミセス・フ
ラジエ（ＩＦＯ ３４３９），糸状菌としてのアスペル
ギルス・オリザエ（ＩＦＯ ５２３９）をそれぞれ米、
米ヌカ、フスマで培養し菌密度が１０９ｃｆｕ／資材１
ｇ（メタリジュウム菌），１０８ｃｆｕ／資材１ｇ（酵
母菌），１０７ｃｆｕ／資材１ｇ（酵母菌），１０６ｃ
ｆｕ／資材１ｇ（放線菌），１０８ｃｆｕ／資材１ｇ
（糸状菌）の培養物を得た。これらを重量比でメタリジ
ュウム菌３：酵母菌１：酵母菌０．５：放線菌０．５：
糸状菌１：パーライト４の割合で混合しその混合物１０
０ｇを３．３ｍ２の土壌に混和しメタリジュウム菌の土
壌定着度合いを試験した。対照区には米に培養したメタ
リジュウム菌（菌密度１０９ｃｆｕ／資材１ｇ）を用い
た。Example 4 Metallidium anisopriae (IFO 31682) (metalridium) as an insect sclerosis bacterium and Saccharomyces cerevisiae (IFO234) as a yeast.
2), Candida tropicalis as a yeast (IF
O 0618), Streptomyces flazier (IFO 3439) as actinomycete, and Aspergillus oryzae (IFO 5239) as filamentous fungus, respectively,
Cultivated with rice bran and bran, the bacterial density is 10 9 cfu / Material 1
g (metallidium), 10 8 cfu / material 1 g (yeast), 10 7 cfu / material 1 g (yeast), 10 6 c
fu / material 1g (actinomycetes), 10 8 cfu / material 1g
A (filamentous) culture was obtained. These are in a weight ratio of metallidium 3: yeast 1: yeast 0.5: actinomycete 0.5:
Filamentous fungus 1: Perlite 4 mixed in a ratio of 10
0 g was mixed with 3.3 m 2 of soil to test the degree of soil colonization of the metal bacterium. For the control group, a metallurgium bacterium cultured in rice (bacterial density 10 9 cfu / 1 g of material) was used.
【００２１】この両者について、土壌１ｇ当たりのメタ
リジュウム菌（昆虫硬化病菌）生息数を調べた。結果を
表４に示す。[0021] For both of these, the number of infestation of metallidium bacterium (insect sclerosis bacterium) per gram of soil was examined. The results are shown in Table 4.
【００２２】[0022]
【表４】 [Table 4]
Claims (4)
ぶ微生物の１種又は２種以上と昆虫硬化病菌とを植物性
有機物にて発酵して得られる発酵物及び天然鉱物若しく
はその加工品を含むことを特徴とする土壌害虫抑制資
材。1. A fermented product obtained by fermenting one or more kinds of microorganisms selected from bacteria, yeasts, actinomycetes and filamentous fungi with insect sclerosis fungi in a plant organic matter and natural minerals or processed products thereof. A soil pest control material, which comprises:
の配合割合が重量比で９９：１〜１：９９であることを
特徴とする請求項１記載の土壌害虫抑制資材。2. The soil pest control material according to claim 1, wherein the mixing ratio of the fermented product and the natural mineral or a processed product thereof is 99: 1 to 1:99 by weight.
ュウム属又はパエシロミセス属に属する微生物であり、
天然鉱物がパーライト、ゼオライト又はパーミキュライ
トである請求項１又は２記載の土壌害虫抑制資材。3. The insect sclerosis bacterium is a microorganism belonging to the genus Beauveria, the genus Metalridium or the genus Paecilomyces,
The soil pest control material according to claim 1 or 2, wherein the natural mineral is perlite, zeolite or permiculite.
土壌害虫抑制資材を土壌に施用することを特徴とする土
壌害虫抑制方法。4. A method for controlling soil pests, which comprises applying the soil pest control material according to any one of claims 1 to 3 to soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5211111A JPH0764698B2 (en) | 1993-08-04 | 1993-08-04 | Soil pest control material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5211111A JPH0764698B2 (en) | 1993-08-04 | 1993-08-04 | Soil pest control material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0748216A JPH0748216A (en) | 1995-02-21 |
| JPH0764698B2 true JPH0764698B2 (en) | 1995-07-12 |
Family
ID=16600596
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5211111A Expired - Fee Related JPH0764698B2 (en) | 1993-08-04 | 1993-08-04 | Soil pest control material |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0764698B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109219352B (en) | 2016-04-28 | 2021-06-29 | 组合化学工业株式会社 | Microbial pesticide formulation composition, its preparation method and use method |
| KR102435838B1 (en) | 2016-04-28 | 2022-08-23 | 구미아이 가가쿠 고교 가부시키가이샤 | Agrochemical formulation composition using Trichoderma bacterium, its preparation and application method |
| CN110896964A (en) * | 2019-12-19 | 2020-03-24 | 河南农贝得农业科技有限公司 | Seed dressing agent without pesticide residue and preparation method thereof |
-
1993
- 1993-08-04 JP JP5211111A patent/JPH0764698B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0748216A (en) | 1995-02-21 |
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