JPH0790300A - Washing agent and washing method - Google Patents

Abstract

PURPOSE:To obtain a washing agent containing a lipid decomposing enzyme, etc., having activity under weak alkaline conditions, having good finishing and useful for cloth and clothing, etc. CONSTITUTION:This washing agent contains one or more kinds of (A) lipid decomposing enzymes (lipase) having activity under weak alkali conditions and (B) cellulose decomposing enzymes (cellulase) having activity under weak alkali conditions. Furthermore, 30-8000LU/L of one or more kinds of lipid decomposing enzymes (lipase) having activity under weak alkali conditions and/or 5-200CU/L of one or more kinds of cellulose decomposing enzymes (cellulase) having activity under weak alkali conditions is preferably added to a washing liquid.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a laundry rinsing agent for clothes, clothes and the like, and a method for washing and rinsing it, and more particularly to a lipolytic enzyme (hereinafter referred to as lipase) having activity under weak alkaline conditions and a weak alkaline condition. A rinsing agent containing at least one cellulolytic enzyme having the following activity (hereinafter, referred to as cellulase) and at least one lipase or cellulase, or a predetermined amount of both of them is added to the washing rinsing solution for rinsing. A method of washing and rinsing characterized by performing.

[0002]

2. Description of the Related Art The addition of enzymes to detergents used for washing clothes, clothes, etc. has been widely practiced. However, enzymes that are proteins are generally relatively unstable substances, and in particular, they are highly alkaline and have a high concentration of an anionic surfactant having a strong enzyme inhibitory action, and
Under the washing conditions in which various additives are present, it is susceptible to decomposition and it is extremely difficult to sufficiently exhibit the original performance of the enzyme. Therefore, the kind of enzyme that can be actually used is significantly restricted.

Therefore, an enzyme having a comparatively stable and high activity under such severe conditions has been actively developed, and an enzyme having an excellent function has been found. It is not always possible to say that the situation is completely satisfactory. Also, in order to utilize the enzyme function as much as possible, it has been proposed to use the enzyme in "pre-soaking" in the pre-washing step where there is no obstructive detergent, but in reality it may be time-consuming. Not popular. On the other hand, as a post-washing step, a cationic surfactant for the purpose of softening is added to the wash rinsing liquid, but addition and use of enzymes are known as far as the present inventors know. There isn't.
Most of the enzymes that have hitherto been widely used as detergent enzymes for cloth, clothes, etc. are proteolytic enzymes (proteases), and there are relatively few cases where lipase or cellulase is used as a detergent enzyme.

[0004]

[Problems to be Solved by the Invention]

1. Removal of lipid stains Lipid stains on clothes are difficult to remove sufficiently by general washing with an anionic surfactant, so a lipase-added detergent was developed. 63-6869
7, US Patent 4011169, British Patent 129361
3) As described above, under conditions where a highly alkaline and high-concentration anionic surfactant is present, it is difficult to sufficiently exhibit its function, and lipase is inactivated by additives such as bleaching agents and fluorescent dyes. Further, lipase, which is a protein itself, may be decomposed and inactivated by a proteolytic enzyme (protease) widely used as a detergent component. Therefore,
In many cases, the removal of lipid stains is not sufficient even with the use of a lipase-mixed detergent.

2) Softness of dyed cotton cloth Generally, when dyed cotton cloth is repeatedly washed, its color becomes whitish and its flexibility is lost. A conventional rinse agent containing a cationic surfactant as an active ingredient recovers flexibility to some extent, but not color tone. A method of recovering flexibility by incorporating cellulase into a detergent has been proposed (British Patent 1368).
599, Japanese Examined Patent Publication No. 61-16316), in the presence of a highly alkaline and high-concentration anionic surfactant as in the case of the lipase described above, the function of cellulase is not sufficiently exerted, and a proteolytic enzyme which is a detergent additive component. It may be inactivated by protease, bleaching agent, fluorescent dye, fragrance, etc., and the expected effect is not obtained. In view of this situation,
The problem to be solved by the present invention is to remove lipid stains adhering to cloth, clothes and the like and to restore the flexibility of dyed cotton cloth,
The aim is to establish a method for effectively recovering the white color.

[0006]

Means for Solving the Problems The inventors of the present invention have conducted various studies to solve the above problems, and as a result, by using lipase in a rinsing step in which the conditions where the function of lipase is easily exhibited sufficiently, lipid stains on clothes can be obtained. Was effectively removed, and by using cellulase in the washing and rinsing process, the softness of the dyed cotton cloth was restored, and the whitish color tone was restored. That is, the present invention provides 30 to 8,000 LU of at least one lipase in the wash rinsing solution.
/ L and / or at least one cellulase of 5 to 20
The present invention provides a laundry rinsing method characterized by adding 0 CU / L, and a laundry rinsing agent containing at least one of lipase and cellulase used for such purpose.

The present invention will be described in more detail below. The lipase used in the present invention is weakly alkaline (p
A lipase having an activity of about H 7.0 to 8.0) can be used without any limitation, but a lipase (triacylglycerol lipase) having a high triglyceride decomposing ability is particularly preferable. It should be noted that even a lipase that exhibits the highest activity under acidic or highly alkaline conditions can naturally be used as long as it is weakly alkaline and exhibits sufficient activity for practical use.

Typical examples of the lipase used in the present invention include, for example, lipase (Pancreatic) extracted from pig pancreas, which is of animal origin.
lyase), lipase extracted from wheat germ as plant-derived one, and Candida cylindrace as microbial-derived one.
cea) produced by Candida lipolytica, Rhizopus lipase produced by Candida lipolytica
lipase produced by arrhizus), Rhizopus
Rhizop, a lipase produced by Rhizopus delemar
lipase produced by us niveus, Chromodoacte-ri
um Viscosum) -produced lipase, Mucor miehei-produced lipase, Mucor pusil (Mucor pusil)
Las) -produced lipase, Pseudomonas mephitica
Produced by Humicola lanuginosa (Hum
Humicola insolens (Humicola i), a lipase produced by icola lanuginosa
Humicola brevispor, lipase produced by Nsolens
a) lipase produced by Fusarium oxysporum, Aspergillus niger
Aspergillus oryze, a lipase produced by L. niger
The lipase produced by a) can be used. As a matter of course, it is desirable that these lipases be used in the highest possible purity by removing the coloring components.

The amount of lipase used in the present invention is not necessarily strictly limited, but as one guideline,
The initial lipase activity in the rinse solution is 30 to 8,000 LU per liter, more preferably 300 to 4,
It is suitable to use it so that it becomes 000 LU. The unit LU (Lipase Unit) of lipase activity is
Tributyrin with PVA as an emulsifier, pH 7, temperature 37
When it is allowed to act at 0 ° C, the titer of the enzyme that releases 1.0 micromolar equivalent of fatty acid per minute is defined as 1 unit. (See page 230, Enzyme Handbook, Jijijinkan, 1981)

The cellulase used in the present invention can be used without limitation as long as it is a cellulase having a weak alkaline and ability to decompose crystalline cellulose. Typical examples of the cellulase used in the present invention are shown in, for example, Trichoderma viride (Tr
Aspergillus niger (Aspergillus niger) produced by ichoderma viride
cellulase produced by Aspergillus sojae, Aspergillus terreus (Asp) produced by Aspergillus sojae
ergillus terreus cellulase, Penicillium funiculosum
cellulase produced by ium funiculosum, penicillium
Humicola isolen, a cellulase produced by M. pusillum.
cellulase produced by s), a cellulase produced by Fusarium moniliforme, Rhizopus delemer (Rhizop)
cellulase produced by Us delemar, Sporotrichum dimorphosporum (Sporotric)
and cellulase produced by hum dimorphosporum) can be used. It is desirable that these cellulases be used in the highest possible purity by removing the coloring components as in the case of lipase.

The amount of cellulase used in the present invention is not strictly limited, but one guideline is as follows.
It is used so that the initial cellulase activity in the rinse solution is 5 to 200 CU per liter, more preferably 20 to 80 CU. The unit of cellulase activity, CU (C
ellulase Unit) has an etherification degree of 0.5
Using CMC of 5 to 0.65 as a substrate, temperature 50 ° C, pH
When 7 is used, the titer of the enzyme that liberates reducing sugar equivalent to 1.0 micromolar equivalent of glucose per minute is 1 CU (see Handbook of Enzyme Use, Jijijinkan, 1981, p. 301). .

The rinsing agent according to the present invention is used as a liquid or granular composition containing, as a main component, at least one of lipase and cellulase, or both. In the case of a liquid, an aqueous solution of a diol or a polyol such as ethylene glycol is used to measure the storage stability of the enzyme. Depending on the enzyme, it may be effective to add a small amount of calcium salt, borate, formic acid, or a neutral surfactant as a storage stabilizer. If necessary, the enzyme can be microencapsulated or suspended in glycol to improve storage stability.

Since the enzyme is relatively stable in a dry state, the storage stability of the granule is good, but the solubility must be taken into consideration. Lactose, starch, sodium chloride, sodium sulfate and the like can be used as the excipient. Further, in order to improve the degradability of the granule and the dispersibility of the enzyme, it is possible to use a small amount of a neutral surfactant or a cationic surfactant. Further, in order to improve the washing finish, a small amount of perfume, bleaching agent, fluorescent dye or the like can be added to the rinse agent of the present invention as long as it does not inhibit the enzyme.

The composition of lipase and cellulase in the preparation is not necessarily limited, but normally, lipase is usually 300 to 80,000 LU per 1 ml of the preparation,
More preferably 3,000-40,000 LU,
The composition of cellulase is 50 ~ per 1 ml of the preparation.
It is suitable to use 2,000 CU, more preferably 200 to 800 CU.
000 to 15,000 times, usually diluted about 10,000 times, that is, an amount of about 1 milliliter per 10 liters of the washing and rinsing liquid is used.

[0015]

EXAMPLES The present invention will be described more specifically by showing typical examples below. However, these are merely examples for facilitating the understanding of the present invention, and therefore,
Of course, the present invention is not limited to these,
It goes without saying that these examples are not limiting in any way.

Example 1 As a test cloth, a cotton cloth having a size of 10 cm × 10 cm impregnated with 300 mg of olive oil and left at room temperature for about 1 week was used. The amount of olive oil impregnated was accurately recorded for each test cloth when weighed with fat. When this test cloth was extracted with normal hexane using a Soxhlet extractor and olive oil was quantified by high performance liquid chromatography, the recovery rate was 96% on average. This test cloth
Washed on Terg-0-Tometer tester.

The washing conditions are as follows: Detergent: JIS K3371 phosphorus-free detergent Detergent concentration: 0.133% Temperature: 30 ° C. Time: 15 minutes Reversal number: 105 CPM Bath ratio: 6 sheets / 1000 ml Incidentally, JISK3371 description The detergent composition is LAS15
%, Zeolite 17%, sodium silicate 5%, sodium carbonate 3%, CMC 1%, and the remaining sodium sulfate.

Next, rinsing was performed using the rinsing agents of the following components. Rinse agent composition: lipase (Candida cylindracea)
Derived): 25,000 LU / ml Cellulase (from Fusarium monoformi): 300 CU / ml Triton X-207: 1.5% by volume calcium acetate: 0.6 g / 1
000 ml Ethylene glycol: 65% by volume Moisture: Balance Rinsing conditions are as follows: Rinsing agent concentration: 0.01% (1st time) Rinsing time: 5 minutes Inversion number: 105 CPM Repeat number: 3 times However, rinsing agent Was added only for the first rinse. After the rinsing was completed, the test cloth was air dried at room temperature.

After washing, rinsing, and drying were repeated three times by the above operation, the oil and fat remaining on the test cloth was extracted with normal hexane using a Soxhlet extractor. The normal hexane extract was concentrated and then quantified by high performance liquid chromatography according to a conventional method. As a result, when the total amount of triglyceride, diglyceride and monoglyceride was used as the residual oil and fat content, the ratio of the residual oil and fat content to the initial oil and fat content was 31% on average. The initial amount of oil / fat was determined by multiplying the amount of olive oil impregnated with the test cloth by an average recovery rate of 96%.

Example 2 In order to investigate the effect of the rinsing agent, the same operation as in Example 1 was carried out without using the rinsing agent, and the ratio of the residual oil and fat component amount to the initial oil and fat content was 53% on average.

Example 3 A green cotton towel was washed in a commercially available fully automatic washing machine with commercially available powdered soap and dried in the sun. When this washing was repeated 25 times, the towel cloth lost its softness and the green color tone became whitish. This towel cloth is 10cm × 10c
A test cloth was cut into m squares, impregnated with 350 mg of olive oil, and allowed to stand at room temperature for 1 week. The recovery rate of olive oil by normal hexane extraction is
The average was 95%. Using this test cloth, washing was performed under the same conditions as in Example 1 except that the bath ratio was 3 sheets / 1000 ml.

Next, rinsing was performed using a rinsing agent having the following composition. Rinse Agent Composition: Lipase (Pseudomonas mephitica)
Origin: 32,000 LU / ml cellulase (Trichoderma viride)
Origin): 480 CU / ml Triton X-207: 2.5% by volume Ethylene glycol: 62% by volume Moisture: Balance The conditions of rinsing and drying were the same as in Example 1.

After the washing-rinsing-drying operation was repeated three times, the recovery of flexibility and the recovery of color tone were evaluated.
The evaluation results are summarized in Example 4. After evaluating the flexibility and color tone, the test cloth was extracted with normal hexane using a Soxhlet extractor, and the oil and fat content was measured. The ratio of the residual oil and fat content to the initial oil and fat content was 36% on average.

Example 4 Washing-rinsing-drying was performed in the same manner as in Example 3 except that a commercially available softening agent was used. Rinse is 0.022% for the first rinse according to the instructions attached with a commercially available softening agent (main component: cationic surfactant).
(0.22 ml / 1000 ml) was used.

After repeating washing-rinsing-drying three times,
The test cloth obtained was compared with the test cloth obtained in Example 3,
The flexibility and color tone were evaluated by a panel of 12 people.
The results are shown below. (Evaluation of recovery of flexibility-12 people's evaluation distribution) Example 3 sample: 0 unchanged, somewhat good 3, good 6, very good 3 Example 4 sample: 0 unchanged, slightly good 4, good 7, very good Good 1 (Evaluation of color tone recovery-12 people's evaluation distribution) Example 3 sample: unchanged 3, somewhat good 5, good 4 Example 4 sample: unchanged 11, slightly good 1, good 0

The results of the evaluation by the panel show that the softening agent has the same effect as that of the cationic softening finishing agent, but the rinsing agent of the present invention has an excellent effect of restoring the color tone. There is. After the panel evaluation was completed, the sample of Example 4 was extracted with normal hexane using a Soxhlet extractor to quantify the residual oil and fat content. The ratio of the residual oil / fat content to the initial oil / fat content was 59% on average.

Claims (2)

[Claims] At least one of a lipolytic enzyme (lipase) having an activity under weak alkaline conditions and a cellulolytic enzyme (cellulase) having an activity under weak alkaline conditions.
Laundry rinse containing seeds. 2. At least one of lipolytic enzymes (lipases) having activity in mildly alkaline conditions in a wash rinsing solution.
30 to 8,000 LU / L and / or 5 to 200 CU / L of at least one cellulolytic enzyme (cellulase) having activity under weakly alkaline conditions, and rinsing is carried out, characterized by comprising rinsing .