JPH0817813B2 - Blood component separation equipment - Google Patents

Blood component separation equipment

Info

Publication number
JPH0817813B2
JPH0817813B2 JP62-505965A JP50596587A JPH0817813B2 JP H0817813 B2 JPH0817813 B2 JP H0817813B2 JP 50596587 A JP50596587 A JP 50596587A JP H0817813 B2 JPH0817813 B2 JP H0817813B2
Authority
JP
Japan
Prior art keywords
blood
fibers
component separation
separation device
blood cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP62-505965A
Other languages
Japanese (ja)
Other versions
JPH0817813B1 (en
JPWO1988002264A1 (en
Inventor
啓次 直井
克彦 岩田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Terumo Corp
Original Assignee
Terumo Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Terumo Corp filed Critical Terumo Corp
Priority to JP62-505965A priority Critical patent/JPH0817813B2/en
Publication of JPWO1988002264A1 publication Critical patent/JPWO1988002264A1/en
Publication of JPH0817813B1 publication Critical patent/JPH0817813B1/ja
Publication of JPH0817813B2 publication Critical patent/JPH0817813B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3627—Degassing devices; Buffer reservoirs; Drip chambers; Blood filters
    • A61M1/3633—Blood component filters, e.g. leukocyte filters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D39/00—Filtering material for liquid or gaseous fluids
    • B01D39/08—Filter cloth, i.e. woven, knitted or interlaced material
    • B01D39/083—Filter cloth, i.e. woven, knitted or interlaced material of organic material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D39/00—Filtering material for liquid or gaseous fluids
    • B01D39/08—Filter cloth, i.e. woven, knitted or interlaced material
    • B01D39/086—Filter cloth, i.e. woven, knitted or interlaced material of inorganic material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/04—Liquids
    • A61M2202/0413—Blood
    • A61M2202/0439—White blood cells; Leucocytes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
    • B01D2239/12—Special parameters characterising the filtering material
    • B01D2239/1233—Fibre diameter

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Vascular Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Inorganic Chemistry (AREA)
  • Cardiology (AREA)
  • Anesthesiology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • External Artificial Organs (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Description

【発明の詳細な説明】 技術分野 本発明は、血液成分分離用器具に関するものである。
詳しく述べると、本発明は、赤血球濃厚液等の血球浮遊
液中に混入する白血球を除去する場合において、簡単な
操作で量、時間の両面で効率よく除去でき、しかもフィ
ルター繊維片の流出の虞れの小さい血液成分分離用器具
に関するものである。Description: TECHNICAL FIELD The present invention relates to an instrument for separating blood components.
More specifically, the present invention relates to a blood component separation device that can remove leukocytes contaminated in a blood cell suspension such as a red blood cell concentrate efficiently in terms of both quantity and time with a simple operation and with little risk of filter fiber pieces leaking out.

背景技術 近年、軟質血液バッグの発達と普及によって、患者に
必要とする血液成分のみを輸血する血液成分輸血療法が
活発に行なわれるようになり、この目的のため、献血に
よって得られた血液は、遠心操作によって赤血球濃厚液
（CRC）、濃縮血小板血漿（PC）および乏血小板血漿（P
PP）などに分離されている。BACKGROUND ART In recent years, with the development and widespread use of flexible blood bags, blood component transfusion therapy, in which only the blood components required by the patient are transfused, has become more common. For this purpose, blood obtained through blood donations is centrifuged to produce packed red blood cells (CRC), platelet-rich plasma (PC), and platelet-poor plasma (PRP).
PP) and other substances.

このようにして分離された赤血球濃厚液は、赤血球の
成分製剤として赤血球を必要とする患者への成分輸血に
広く用いられているが、実際には赤血球濃厚液は、多く
の白血球、血小板を含み、いわゆる全成分血液であると
考え方が定着しつつあり、赤血球のみを必要としている
患者への赤血球濃厚液の輸血により併せて多量の白血球
および血小板が輸血されていることが問題視されてい
る。 The red blood cell concentrate separated in this manner is widely used as a red blood cell component preparation for component transfusion to patients who require red blood cells. However, in reality, red blood cell concentrate contains many white blood cells and platelets, and the idea that it is so-called whole blood component is becoming established. Therefore, it is problematic that when red blood cell concentrate is transfused to a patient who requires only red blood cells, a large amount of white blood cells and platelets is also transfused.

赤血球濃厚液等の血球浮遊液から白血球と血小板を除
く方法としては、CRCのような白血球含有浮遊液中にデ
キストランまたはヒドロキシエチルスターチ等の赤血球
凝集剤を添加し、凝集剤導入溶液を沈降させ溶液を赤血
球沈降と白血球含有溶液相とに分離する方法があるが、
手間が掛かる事、白血球の除去効率が悪い等の問題があ
り、現在は、繊維を充填したカラムに血球浮遊液を流
し、そこに白血球・血小板を吸着除去する方法が主に行
なわれている。この方法においては、繊維の充填密度を
上げることにより白血球・血小板の除去率を高くするこ
とができるが、半面過時間も長くなり、血球除去率と
血球浮遊液処理時間とを良好なものとして両立させる事
は、困難であった。この点を解決するために極細繊維そ
のもの、またはその不織布を材として用いる方法が提
唱されている（特公昭58−54,126号、特開昭60−193,46
8号）が、極細繊維そのものを充填した場合は、前述の
血球除去率と処理時間のバランスの問題が完全には解決
出来ず、繊維のつめ方によりチャンネリングが発生す
る、繊維片の流出の虞れがあるなどの問題点の残るもの
であり、また不織布として用いた場合も繊維同士を熱固
着、溶着材による固定などを行なわない限り、絡合が不
十分なため繊維片の流出の可能性があるものであった。 One method for removing leukocytes and platelets from a blood cell suspension such as a red blood cell concentrate is to add a red blood cell agglutinant such as dextran or hydroxyethyl starch to a white blood cell-containing suspension such as CRC, and then allow the agglutinant-introduced solution to settle, separating the solution into a red blood cell sedimentation phase and a white blood cell-containing solution phase.
However, due to problems such as the time-consuming nature of the method and the low efficiency of leukocyte removal, the currently most common method involves passing a blood cell suspension through a column packed with fibers, where the leukocytes and platelets are adsorbed and removed. In this method, the removal rate of leukocytes and platelets can be increased by increasing the fiber packing density, but the elapsed time is also lengthened, making it difficult to achieve both a good blood cell removal rate and a good blood cell suspension treatment time. To solve this problem, a method has been proposed in which ultrafine fibers themselves or nonwoven fabrics thereof are used as materials (Japanese Patent Publication No. 58-54,126, Japanese Patent Laid-Open No. 60-193,46).
However, when ultrafine fibers themselves are filled, the problem of the balance between the blood cell removal rate and the processing time mentioned above cannot be completely solved, and problems remain such as channeling occurring depending on how the fibers are packed and the risk of fiber fragments leaking out. Furthermore, when used as a nonwoven fabric, unless the fibers are thermally bonded together or fixed with a welding material, there is a possibility of fiber fragments leaking out due to insufficient entanglement.

従って本発明は、改良された血液成分分離用器具を提
供することを目的とする。 SUMMARY OF THE INVENTION It is therefore an object of the present invention to provide an improved device for separating blood components.

本発明はまた、赤血球濃厚液等の血球浮遊液中に混入
する白血球および血小板を除去する場合において、簡単
な操作で量、時間の両面で効率よく除去でき、しかもフ
ィルター繊維片の流出の虞れの小さい血液成分分離用器
具を提供することを目的とする。 Another object of the present invention is to provide a blood component separator which, when removing leukocytes and platelets mixed in a blood cell suspension such as a red blood cell concentrate, can efficiently remove them in terms of both quantity and time with a simple operation and with little risk of filter fiber pieces leaking out.

発明の開示 上記諸目的は、血球浮遊液流入口と血球浮遊液流出口
とを備えたハウジングと、実質的に血球浮遊液を変質さ
せない性質の複数の繊維に縒りをかけることにより得ら
れた糸の織物および編物よりなる群から選ばれた少なく
とも１種のハウジング内に0.2〜1.0g/cm3の嵩密度で充
填されたフィルター材とからなることを特徴とする血液
成分分離用器具により達成される。Disclosure of the Invention The above objects are achieved by a blood component separation device comprising: a housing having an inlet for a blood cell suspension and an outlet for a blood cell suspension; and a filter material filled into the housing at a bulk density of 0.2 to 1.0 g/ cm3 , the filter material being of at least one kind selected from the group consisting of woven and knitted fabrics made of yarn obtained by twisting a plurality of fibers that have properties that do not substantially alter the blood cell suspension.

本発明はまた、織物または編物の目の大きさが赤血球
を通過させ得るものである血液成分分離用器具を示すも
のである。本発明はさらに織物または編物の目が直径10
〜200μｍ程度のものである血液成分分離用器具を示す
ものである。本発明はまた、繊維が、合成繊維、半合成
繊維、再生人造繊維、無機繊維または天然繊維である血
液成分分離用器具を示すものである。本発明は、繊維
が、ポリアミド系、ポリエステル系、ポリアクリロニト
リル系、ポリスチレン系、ポリオレフィン系、ポリウレ
タン系、およびアセテート系よりなる群から選ばれたも
のである血液成分分離用器具を示すものである。本発明
はさらにまた、織物または編物が繊維の直径10〜50μｍ
程度の糸により構成されているものである血液成分分離
用器具を示すものである。本発明はまた、織物または編
物が繊維の直径10〜30μｍ程度の糸により構成されてい
るものである血液成分分離用器具を示すものである。本
発明はさらに、織物または編物の目が直径10〜100μｍ
程度のものである血液成分分離装置を示すものである。 The present invention also provides a blood component separation device in which the size of the openings in the woven or knitted fabric is large enough to allow red blood cells to pass through.
The present invention also relates to a blood component separation device in which the fibers are synthetic fibers, semi-synthetic fibers, regenerated artificial fibers, inorganic fibers, or natural fibers. The present invention also relates to a blood component separation device in which the fibers are selected from the group consisting of polyamides, polyesters, polyacrylonitriles, polystyrenes, polyolefins, polyurethanes, and acetates. The present invention still further relates to a blood component separation device in which the woven or knitted fabric has a fiber diameter of 10 to 50 μm.
The present invention also relates to a blood component separation device in which the woven or knitted fabric is made of threads having a fiber diameter of about 10 to 30 μm. The present invention further relates to a blood component separation device in which the woven or knitted fabric is made of threads having a fiber diameter of about 10 to 100 μm.
This shows a blood component separation device of this order.

図面の簡単な説明 第１図は、本発明の血液成分分離用器具の一実施態様
を示す断面図であり、また 第２図は本発明の血液成分分離用器具を組込んだ処理
回路を示す図面である。BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a cross-sectional view showing one embodiment of the blood component separation device of the present invention, and FIG. 2 is a diagram showing a processing circuit incorporating the blood component separation device of the present invention.

発明を実施するための最良の形態 本発明の血液成分分離用器具は、実質的に血球浮遊液
を変性させない繊維に縒りをかけることによって得られ
た糸の織物または編物をフィルター材として用いること
を特徴とするものである。織物、編物には繊維の交差部
に“目”があり、この“目”がフィルター材として用い
られた場合が流路となる。しかして、織物、編物の場
合、この“目”は整然と配列されているので、織物また
は編物を積層して圧迫したとしても（すなわち充填密度
を上げても）、この目は確保され、赤血球濃厚液等の血
球浮遊液を迅速に流す血液成分分離用器具として有用な
効果を表わすものである。BEST MODE FOR CARRYING OUT THE INVENTION The blood component separation device of the present invention is characterized by using, as a filter material, a woven or knitted fabric made of yarn obtained by twisting fibers that do not substantially denature blood cell suspensions. Woven and knitted fabrics have "mesh" at the intersections of the fibers, and when used as a filter material, these "mesh" form flow paths. In the case of woven and knitted fabrics, these "mesh" are arranged in an orderly manner, so that even when the woven or knitted fabrics are stacked and compressed (i.e., even when the packing density is increased), these "mesh" remain intact, providing a useful effect as a blood component separation device that allows blood cell suspensions such as concentrated red blood cells to pass through quickly.

また繊維そのものを雑然と充填するよりも織物、編物
とした方が、充填効率がよく、より高い充填密度で充填
でき、同一容積においても不要な血球成分除去効率を上
げることができるものである。 Furthermore, packing efficiency is better and the packing density is higher when the fibers are woven or knitted rather than when the fibers themselves are packed randomly, and the efficiency of removing unnecessary blood cell components can be increased even for the same volume.

繊維による白血球および血小板の捕捉機構としては、
白血球および血小板の繊維に対する粘着および繊維と繊
維の隙間に白血球および血小板がひっかかることによる
捕捉が考えられる。特公昭58−54,126号においては、極
細繊維を用いることで繊維間の隙間の距離を小さくし、
また表面積を大きくして捕捉効率を高めている。このよ
うな極細繊維を用いる方法の他に、繊維間の隙間の距離
を小さくし表面積を大きくして捕捉効率を高める方法と
しては、フィルター材を圧縮して充填密度を上げること
が考えられる。しかしながら、繊維そのものを充填して
圧縮し充填密度を上げれば過速度が極端に遅くなり血
液成分分離用器具として満足できないものとなる。これ
は繊維そのものを雑然と充填するために、血球浮遊液の
流路が確保し難いために生じる現象と考えられた。そこ
で本発明者らは、血球浮遊液の流路をより確保し易いと
考えられる繊維の織物、編物に注目し、上記のごとく本
発明に到ったものである。 The mechanisms by which fibers capture leukocytes and platelets include:
It is thought that the adhesion of leukocytes and platelets to fibers and the capture of leukocytes and platelets by getting caught in the gaps between fibers are responsible for this. In Japanese Patent Publication No. 58-54126, ultrafine fibers are used to reduce the gaps between fibers,
Furthermore, the surface area is increased, thereby improving the capture efficiency. In addition to the method of using such ultrafine fibers, another method of increasing the capture efficiency by reducing the gap distance between fibers and increasing the surface area is to compress the filter material to increase the packing density. However, if the fibers themselves are packed and compressed to increase the packing density, the overflow rate becomes extremely slow, resulting in an unsatisfactory blood component separation device. This phenomenon is thought to occur because the fibers themselves are packed in a disorderly manner, making it difficult to ensure a flow path for the blood cell suspension. Therefore, the inventors focused on woven and knitted fibers, which are thought to more easily ensure a flow path for the blood cell suspension, and arrived at the present invention as described above.

以下、本発明の実施態様に基づき詳細に説明する。The present invention will be described in detail below based on embodiments.

本発明の血液成分分離用器具は実質的に血球浮遊液成
分を変性させない繊維に縒りをかけることによって得ら
れた糸の織物および編物よりなる群から選ばれた少なく
とも１種のハウジング内に充填されたフィルター材を有
することを特徴とするものである。 The blood component separation device of the present invention is characterized by having a filter material filled in a housing of at least one material selected from the group consisting of woven and knitted fabrics made of yarn obtained by twisting fibers that do not substantially denature blood cell suspension components.

織物および編物を構成する繊維の材質としては、実質
的に血球浮遊液成分を変性させないものであれば特に限
定されず、ポリアミド系、ポリエステル系、ポリアクリ
ロニトリル系、ポリスチレン系、ポリオレフィン系、ポ
リウレタン系などの合成繊維、アセテート系などの半合
成繊維、銅アンモニアセルロースなどの再生人造繊維、
ガラス繊維などの無機繊維、綿、絹糸、羊毛などの天然
繊維等各種のものが用いられるが、特にポリアミド系、
ポリエステル系、ポリアクリロニトリル系、ポリスチレ
ン系、ポリオレフィン系、ポリウレタン系およびアセテ
ート系のものが好ましい。 The material of the fibers constituting the woven and knitted fabrics is not particularly limited as long as it does not substantially denature the components of the blood cell suspension, and examples thereof include synthetic fibers such as polyamides, polyesters, polyacrylonitriles, polystyrenes, polyolefins, and polyurethanes, semi-synthetic fibers such as acetates, regenerated artificial fibers such as cuprammonium cellulose,
Various types of fibers are used, including inorganic fibers such as glass fiber, and natural fibers such as cotton, silk, and wool. In particular, polyamides,
Polyester-based, polyacrylonitrile-based, polystyrene-based, polyolefin-based, polyurethane-based and acetate-based materials are preferred.

このような材質からなる繊維から構成される織物また
は編物の目の大きさは、血球浮遊液を通過させた際充分
な流速を確保できかつ該血球浮遊液との接触の機会が充
分であることが好ましく、通過させる血球浮遊液が赤血
球を多く含む場合は赤血球を十分通過させ得るものであ
ることが好ましく、具体的には直径10〜200μｍ、さら
に望ましくは10〜100μｍ程度であることが好ましい。
この場合、赤血球を通過させ得るとは、赤血球が変形し
て通過することができる場合を含む。 The mesh size of the woven or knitted fabric made of fibers of such materials is preferably such that a sufficient flow rate can be ensured when a blood cell suspension is passed through and that there is sufficient opportunity for contact with the blood cell suspension. When the blood cell suspension to be passed through contains a large amount of red blood cells, the mesh size is preferably such that the red blood cells can be sufficiently passed through. Specifically, the mesh size is preferably about 10 to 200 μm, more preferably about 10 to 100 μm in diameter.
In this case, being able to allow red blood cells to pass through includes the case where red blood cells are able to pass through after being deformed.

織物または編物を構成する繊維の糸の太さは、上記の
ごとき目の大きさを確保できるものであれば特に限定さ
れないが、織物または編物とする製造性の面からおよび
充填密度を高めるうえから、通常直径200〜1500μｍ、
より好ましくは200〜1000μｍ程度のものとされる。ま
た繊維の糸は、複数のフィラメントからなる糸であり、
モノフィラメントは10〜50μｍ、より好ましくは10〜30
μｍのものとされる。 The thickness of the fiber threads constituting the woven or knitted fabric is not particularly limited as long as it can ensure the above-mentioned mesh size. However, from the viewpoint of manufacturability as a woven or knitted fabric and for increasing the packing density, the diameter is usually 200 to 1500 μm.
More preferably, the fiber thread is a thread made of a plurality of filaments, and the fiber thread is a thread made of a plurality of filaments.
The monofilament is 10 to 50 μm, more preferably 10 to 30
It is said to be in the μm range.

第１図は、本発明の血液成分分離用器具の一実施態様
を示すものである。この実施態様において、血液成分分
離用器具１は、丸盆状ハウジング部材2,2aが内部に空間
部を形成するように外周部の嵌合部3,3aにおいて液密に
嵌合され、該内部空間部にはメッシュ状支持材4,4aに挟
まれて、複数枚の織物および／または編物が積層されて
なるフィルター材が設けられて、構成されている。なお
一方のハウジング部材２の中央部には血球浮遊液流入口
６が、他方のハウジング部材2aの中央部には血球浮遊液
流出口７がそれぞれ内部空間に連通するように設けられ
ている。また、支持体4,4aは、例えばポリエステル等の
材質よりなり、複数枚の織物および／または編物よりな
るフィルター材５を支持すると同時に、メッシュ状とさ
れて導入出流体を均一流とする流路規制部の役目を兼ね
るものである。このような血液成分分離用器具１におい
て、織物および／または編物よりなるフィルター材５は
繊維の種類、太さなどにも影響されるが嵩密度が0.2〜
1.0g/cm2程度となるように積層されて充填される。フィ
ルター材５の嵩密度は、過される血球浮遊液の過速
度、白血球捕捉能等を大きく左右するものである。また
フィルター材５の充填量は過される血球浮遊液100ml
当り３〜15gになるようにすればよい。 FIG. 1 shows one embodiment of the blood component separation device of the present invention. In this embodiment, the blood component separation device 1 is configured such that round tray-shaped housing members 2, 2a are fitted liquid-tightly at fitting portions 3, 3a on the outer periphery to form an internal space, and a filter material composed of multiple laminated woven and/or knitted fabrics is sandwiched between mesh support members 4, 4a in the internal space. A blood cell suspension inlet 6 is provided in the center of one housing member 2, and a blood cell suspension outlet 7 is provided in the center of the other housing member 2a, both of which communicate with the internal space. The support members 4, 4a are made of a material such as polyester, and support the filter material 5 composed of multiple woven and/or knitted fabrics. Their mesh structure also serves as a flow path regulator to ensure uniform flow of the inlet and outlet fluids. In this blood component separation device 1, the woven and/or knitted filter material 5 has a bulk density of 0.2 to 1.5 mm, depending on the type and thickness of the fiber.
The filter material 5 is packed in layers so that the density is about 1.0 g/ cm² . The bulk density of the filter material 5 greatly affects the flow rate of the blood cell suspension being filtered, the leukocyte capturing capacity, etc. The packed amount of the filter material 5 is 100 ml per 100 ml of blood cell suspension being filtered.
The amount should be 3 to 15g per serving.

このような構成を有する血液成分分離用器具１は、例
えば第２図に示すようにして使用される。 The blood component separating device 1 having such a configuration is used, for example, as shown in FIG.

すなわち、過しようとする血球浮遊液を入れた血球
浮遊液バッグ８および生理食塩水を入れた生理食塩水バ
ッグ９を血液成分分離用器具１より上方に位置させ、そ
れぞれ調整バルブ10a,10bおよび三方コネクター11aを介
し、液体成分分離用器具１の血球浮遊液流入口６に連結
チューブ12aにより接続し、一方血液成分分離用器具１
より下方に位置させた回収バッグ13,14を同様に調整バ
ルブ10c,10dおよび三方コネクター11bを介して血液成分
分離用器具１の血球浮遊液流出口７に連結チューブ12b
により接続して処理回路を形成する。血球浮遊液の処理
は、まず生理食塩水バッグ９より生理食塩水を流して回
路内をプライミングした後、血球浮遊液バッグ８より血
球浮遊液を自然落差法により連結チューブ12aを通じて
血液成分分離用器具１に導入する。血液成分分離用器具
１に導入された血球浮遊液は織物および／または編布よ
りなるフィルター材により白血球、血小板等を捕捉さ
れ、赤血球、血漿が主となるものとなり連結チューブ12
bを通じて回収バッグ13へ回収される。さらに赤血球の
回収率を高めるためには、生理食塩水バッグ９より同様
にして連結チューブ12aを通じて血液成分分離用器具１
に生理食塩水を導入し、血液成分分離用器具１内に一部
残存している赤血球を生理食塩水と共に連結チューブ12
bを通じて他方の回収バッグ14へ回収することがなされ
得る。このように簡単な操作で効率よくかつ短時間で白
血球および血小板を分離除去できる。なお本発明の血液
成分分離用器具による白血球および血小板除去操作に適
用される血球浮遊液としては、血液、腹水や骨髄液等の
その他の体液、および赤血球濃厚液などの遠心分離操作
により得られた血球浮遊液、赤血球凝集剤のような血球
凝集剤を添加して得られた血球浮遊液、細胞電気泳動に
より得られた血球浮遊液などが含まれるものである。 That is, a blood cell suspension bag 8 containing the blood cell suspension to be filtered and a physiological saline bag 9 containing physiological saline are positioned above the blood component separation device 1, and are connected to the blood cell suspension inlet 6 of the liquid component separation device 1 by a connecting tube 12a via adjustment valves 10a, 10b and a three-way connector 11a, respectively.
Similarly, the collection bags 13 and 14 positioned further downward are connected to the blood cell suspension outlet 7 of the blood component separation device 1 via the adjusting valves 10c and 10d and the three-way connector 11b through the connecting tube 12b.
The blood cell suspension is treated by first priming the circuit with physiological saline from the physiological saline bag 9, and then the blood cell suspension is introduced from the blood cell suspension bag 8 into the blood component separation device 1 through the connecting tube 12a by natural head. The blood cell suspension introduced into the blood component separation device 1 has white blood cells, platelets, etc. captured by the filter material made of woven and/or knitted fabric, and the blood suspension becomes mainly composed of red blood cells and plasma, and is then passed through the connecting tube 12a.
In order to further increase the recovery rate of red blood cells, the blood is collected from the physiological saline bag 9 through the connecting tube 12a to the blood component separation device 1.
Physiological saline is introduced into the blood component separation device 1, and the red blood cells remaining in the blood component separation device 1 are separated into the connecting tube 12 together with the physiological saline.
and then collected into the other collection bag 14 through the blood component separation device 10b. In this way, leukocytes and platelets can be separated and removed efficiently in a short time with a simple operation. Blood cell suspensions applicable to the leukocyte and platelet removal operation using the blood component separation device of the present invention include blood, other body fluids such as ascites and bone marrow fluid, blood cell suspensions obtained by centrifugation of red blood cell concentrates, blood cell suspensions obtained by adding a blood cell agglutinating agent such as a red blood cell agglutinating agent, and blood cell suspensions obtained by cell electrophoresis.

以下、本発明を実施例に基づき、さらに具体的に説明
する。 The present invention will be described in more detail below based on examples.

実施例１ 直径12μｍのポリエステルフィラメントからの20tex
の糸を原料とする編物を、第１図に示すようにハウジン
グ空間（内径56mm、フィルター層長21mm）に70枚積層し
て嵩密度0.5g/cm3となるように充填し血液成分分離用器
具を作成した。200mlCPD加血液を入れた血球浮遊液バッ
グ８から落差750mmの位置に上記の血液成分分離用器具
をつけ、更に750mm下方に回収バッグ13（トランスファ
ーバッグＴ−200,テルモ（株）製）をつけそれぞれの間
を内径3mm、外径6mmの塩化ビニル樹脂製チューブで連結
して第２図に示したような処理回路を組みあげた。生理
食塩水バッグ９より約100mlの生理食塩水を流して回路
内をプライミング後自然落差法により血液を血液成分分
離用器具に流し、更に、約100mlの生理食塩水で赤血球
回収操作を実施した。この結果、血液の処理時間は２分
45秒であり、単位面積当りの処理速度は3.0ml/cm2/分
で、また白血球除去率は98.0％、赤血球回収率は95.0％
であった。Example 1 20 tex from polyester filaments with a diameter of 12 μm
A blood component separation device was fabricated by stacking 70 sheets of knitted fabric made from this yarn in a housing space (inner diameter 56 mm, filter layer length 21 mm) as shown in Figure 1, filling it to a bulk density of 0.5 g/ cm³ . The blood component separation device was attached 750 mm down from a blood cell suspension bag 8 containing 200 ml of CPD-added blood, and a collection bag 13 (Transfer Bag T-200, Terumo Corp.) was attached 750 mm further down, and the two bags were connected with polyvinyl chloride resin tubing with an inner diameter of 3 mm and an outer diameter of 6 mm, completing the processing circuit shown in Figure 2. After priming the circuit with approximately 100 ml of saline from the saline bag 9, blood was passed through the blood component separation device by gravity, and red blood cell collection was performed with approximately 100 ml of saline. The blood processing time was 2 minutes.
The time required for the treatment was 45 seconds, the processing speed per unit area was 3.0 ml/cm 2 /min, and the leukocyte removal rate was 98.0% and the red blood cell recovery rate was 95.0%.
It was.

なお白血球除去率および赤血球回収率は、フィルター
通過前と通過後の血液中の赤血球数および白血球数を自
動血算装置（ELT−8,オルソー社製）を用いて算定の
後、液量に基づいて各血球成分の絶対量を求めて算出さ
れた。 The leukocyte removal rate and red blood cell recovery rate were calculated by counting the number of red blood cells and white blood cells in the blood before and after passing through the filter using an automatic blood counter (ELT-8, Ortho), and then calculating the absolute amount of each blood cell component based on the liquid volume.

実施例２ 直径12μｍのポリエステルフィラメントからの20tex
の糸を原料とする織物を90枚積層して0.69g/cm3の嵩密
度となるようにハウジングに充填し、実施例１と同様な
回路・方法で血液を処理した。この結果、血液の処理時
間は３分であり、単位面積当りの処理速度は2.7ml/cm2/
分で、また白血球除去率は99.0％、赤血球回収率は96.0
％であった。Example 2 20 tex from polyester filaments with a diameter of 12 μm
90 sheets of fabric made from this yarn were stacked and packed into a housing to give a bulk density of 0.69 g/cm 3 , and blood was treated using the same circuit and method as in Example 1. As a result, the blood treatment time was 3 minutes, and the treatment rate per unit area was 2.7 ml/cm 2 /
minutes, and the leukocyte removal rate was 99.0% and the red blood cell recovery rate was 96.0%.
%.

比較例１ 直径12μｍのポリエステルフィラメントそのものを実
施例１と同様のハウジング空間に嵩密度0.35g/cm3（ヒ
トの力でこれ以上の嵩密度で充填することは困難であっ
た。）となるように均一に充填し、実施例1,2と同様な
形態の血液成分分離用器具を作成した。その後実施例１
と同様な回路・方法で血液を処理した。この時の血液処
理時間は４分30秒であり、単位面積当りの処理速度は2.
0ml/cm2/分で、また白血球除去率は62％、赤血球回収率
は93％であった。Comparative Example 1: Polyester filaments with a diameter of 12 μm were uniformly packed into the same housing space as in Example 1 to a bulk density of 0.35 g/cm 3 (it was difficult to pack at a bulk density higher than this using human strength), and a blood component separation device of the same configuration as in Examples 1 and 2 was fabricated.
The blood was processed using the same circuit and method as in the previous example. The blood processing time was 4 minutes and 30 seconds, and the processing speed per unit area was 2.
0 ml/cm 2 /min, and the leukocyte removal rate was 62% and the red blood cell recovery rate was 93%.

実施例３ 平均直径16μｍの天然綿フィラメントからの直径20te
xの糸を用いた編物を0.5g/cm3の嵩密度となるようにハ
ウジングに66枚積層して充填し、実施例と同様な回路・
方法で血液を処理した。この結果、血液の処理時間は３
分であり、単位面積当りの処理速度は2.7ml/cm2/分で、
白血球除去率は98.3％、赤血球回収率は96.0％であっ
た。Example 3: 20 te diameter yarns from natural cotton filaments with an average diameter of 16 μm
66 sheets of knitted fabric using yarn x were stacked and packed into a housing so that the bulk density was 0.5 g/cm 3 , and the same circuit and
The blood was treated by the method. As a result, the blood treatment time was 3
The treatment rate per unit area is 2.7 ml/cm 2 /min.
The leukocyte removal rate was 98.3%, and the red blood cell recovery rate was 96.0%.

比較例２ 実施例３と同様の平均直径16μｍの天然綿フィラメン
トそのものを0.35g/cm3（ヒトの力でこれ以上の嵩密度
で充填することは困難であった。）となるように均一に
充填し、実施例１と同様な回路・方法で血液を処理し
た。この時の血液の処理時間は７分30秒であり、単位面
積当りの処理速度は1.1ml/cm2/分で、また白血球除去率
は94.8％、赤血球回収率は93.2％であった。Comparative Example 2: The same natural cotton filaments as in Example 3, each having an average diameter of 16 μm, were uniformly packed to a density of 0.35 g/ cm3 (it was difficult to pack at a higher bulk density using human strength), and blood was treated using the same circuit and method as in Example 1. The blood treatment time was 7 minutes and 30 seconds, the treatment rate per unit area was 1.1 ml/ cm2 /min, the leukocyte removal rate was 94.8%, and the red blood cell recovery rate was 93.2%.

産業上の利用可能性 以上述べたように、本発明は、血球浮遊液流入口と血
球浮遊液流出口とを備えたハウジングと、実質的に血球
浮遊液を変質させない性質の複数の繊維に縒りをかける
ことにより得られた糸の織物および編物よりなる群から
選ばれた少なくとも１種のハウジング内に0.2〜1.0g/cm
3の嵩密度で充填されたフィルター材とからなることを
特徴とする血液成分分離用器具であるから、フィルター
材の充填密度を高くしても処理される血球浮遊液の流路
が確保されており、簡単な操作で、量、時間の両面で効
率よくかつ高捕捉能を有して除去でき、しかも繊維の糸
を織物および／または編物としているために、操作時に
おける繊維片の流出の虞れもなく、また血液のチャンネ
リングの発生も少ない。INDUSTRIAL APPLICABILITY As described above, the present invention provides a method for detecting blood cell suspensions by using a blood cell suspension containing a blood suspension inlet and a blood cell suspension outlet, and a method for detecting blood cell suspensions by using a blood cell suspension containing a blood suspension containing a blood suspension inlet and a blood cell suspension outlet.
Since the blood component separation device is characterized by comprising a filter material packed at a bulk density of 3 , a flow path for the blood cell suspension to be treated is ensured even when the packing density of the filter material is increased, and removal can be performed efficiently in terms of both amount and time with a high capture capacity through simple operation, and further, since the fiber threads are woven and/or knitted, there is no risk of fiber pieces leaking out during operation and there is little occurrence of blood channeling.

本発明の血液成分分離用器具は、織物および／または
編物の目の大きさが赤血球を通過させ得るもの、より好
ましくは直径10〜100μｍのものであると成分織物また
は編物の目による血液成分の流速は充分となりかつ血液
成分と繊維との接触の機会も充分となり、その結果白血
球および血小板の捕捉はより良好なものとなり、また繊
維がポリアミド系、ポリエステル系、ポリアクリロニト
リル系、ポリスチレン系、ポリオレフィン系、ポリウレ
タン系、およびアセテート系よりなる群から選ばれたも
のであると白血球および血小板の繊維に対する粘着によ
る捕捉も良好なものとなるためにさらに捕捉効率は良好
なものとなる。さらに織物または編物が、繊維の直径10
〜30μｍ程度の糸により構成されているものであると、
製造性面からもまた充填密度を上げるうえからもさらに
良好な血液成分分離用器具が得られる。 In the blood component separation device of the present invention, if the mesh size of the woven and/or knitted fabric is such that red blood cells can pass through, more preferably a diameter of 10 to 100 μm, the flow rate of the blood components through the mesh of the woven or knitted fabric will be sufficient and there will be sufficient opportunity for the blood components to come into contact with the fibers, resulting in better capture of leukocytes and platelets.Furthermore, if the fibers are selected from the group consisting of polyamides, polyesters, polyacrylonitriles, polystyrenes, polyolefins, polyurethanes, and acetates, the capture of leukocytes and platelets by adhesion to the fibers will also be good, resulting in even better capture efficiency.Furthermore, if the woven or knitted fabric has a fiber diameter of 10 to 100 μm,
If it is made up of threads of about 30 μm,
A blood component separation device that is more favorable in terms of manufacturability and increased packing density can be obtained.

Claims (7)

【特許請求の範囲】[Claims] 【請求項１】血液浮遊液流入口と血液浮遊液流出口とを
備えたハウジングと、直径が10〜50μｍでありかつ実質
的に血球浮遊液を変質させない性質の複数の繊維に縒り
をかけることにより得られた糸の織物および編物よりな
る群から選ばれた少なくとも１種のハウジング内に0.2
〜1.0g/cm3の嵩密度で充填されたフィルター材とからな
ることを特徴とする血液成分分離用器具。[Claim 1] A blood suspension containing a blood suspension inlet and an outlet, and a 0.2 mm diameter blood suspension in at least one housing selected from the group consisting of woven and knitted yarns obtained by twisting a plurality of fibers having a diameter of 10 to 50 μm and having a property that does not substantially alter the blood suspension.
and a filter material packed at a bulk density of 1.0 g/cm 3 or less. 【請求項２】織物または編物の目の大きさが赤血球を通
過させ得るものである請求の範囲第１項に記載の血液成
分分離用器具。2. The blood component separation device according to claim 1, wherein the size of the openings in the woven or knitted fabric is such that red blood cells can pass through. 【請求項３】織物または編物の目が直径10〜200μｍの
ものである請求の範囲第１項に記載の血液成分分離用器
具。3. The blood component separation device according to claim 1, wherein the woven or knitted fabric has a mesh diameter of 10 to 200 μm. 【請求項４】繊維が合成繊維、半合成繊維、再生人造繊
維、無機繊維または天然繊維である請求の範囲第１項に
記載の血液成分分離用器具。4. The blood component separation device according to claim 1, wherein the fibers are synthetic fibers, semi-synthetic fibers, regenerated artificial fibers, inorganic fibers or natural fibers. 【請求項５】繊維がポリアミド系、ポリエステル系、ポ
リアクリロニトリル系、ポリスチレン系、ポリオレフィ
ン系、ポリウレタン系、およびアセテート系よりなる群
から選ばれたものである請求の範囲第４項に記載の血液
成分分離用器具。5. The blood component separation device according to claim 4, wherein the fibers are selected from the group consisting of polyamides, polyesters, polyacrylonitriles, polystyrenes, polyolefins, polyurethanes, and acetates. 【請求項６】織物または編物が繊維の直径10〜30μｍの
糸により構成されているものである請求の範囲第１項に
記載の血液成分分離用器具。6. The blood component separation device according to claim 1, wherein the woven or knitted fabric is made of threads having a fiber diameter of 10 to 30 μm. 【請求項７】織物または編物の目が直径10〜100μｍの
ものである請求の範囲第１項に記載の血液成分分離用器
具。7. The blood component separation device according to claim 1, wherein the woven or knitted fabric has a mesh diameter of 10 to 100 μm.
JP62-505965A 1986-10-06 1987-10-06 Blood component separation equipment Expired - Lifetime JPH0817813B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62-505965A JPH0817813B2 (en) 1986-10-06 1987-10-06 Blood component separation equipment

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP23760686 1986-10-06
JP61-237606 1986-10-06
PCT/JP1987/000749 WO1988002264A1 (en) 1986-10-06 1987-10-06 Blood component separator
JP62-505965A JPH0817813B2 (en) 1986-10-06 1987-10-06 Blood component separation equipment

Publications (3)

Publication Number Publication Date
JPWO1988002264A1 JPWO1988002264A1 (en) 1988-10-06
JPH0817813B1 JPH0817813B1 (en) 1996-02-28
JPH0817813B2 true JPH0817813B2 (en) 1996-02-28

Family

ID=17017810

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Application Number Title Priority Date Filing Date
JP62-505965A Expired - Lifetime JPH0817813B2 (en) 1986-10-06 1987-10-06 Blood component separation equipment

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Country Link
EP (1) EP0365676B1 (en)
JP (1) JPH0817813B2 (en)
KR (1) KR880701568A (en)
AU (1) AU609741B2 (en)
CA (1) CA1305052C (en)
DE (1) DE3785459T2 (en)
ES (1) ES2005028A6 (en)
WO (1) WO1988002264A1 (en)

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EP0365676B1 (en) 1993-04-14
JPH0817813B1 (en) 1996-02-28
EP0365676A1 (en) 1990-05-02
CA1305052C (en) 1992-07-14
AU8073887A (en) 1988-04-21
WO1988002264A1 (en) 1988-04-07
DE3785459D1 (en) 1993-05-19
EP0365676A4 (en) 1989-11-30
KR880701568A (en) 1988-11-03
AU609741B2 (en) 1991-05-09
DE3785459T2 (en) 1993-08-19
ES2005028A6 (en) 1989-02-16

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