JPH0819372A - Production of silk protein - Google Patents
Production of silk proteinInfo
- Publication number
- JPH0819372A JPH0819372A JP6157608A JP15760894A JPH0819372A JP H0819372 A JPH0819372 A JP H0819372A JP 6157608 A JP6157608 A JP 6157608A JP 15760894 A JP15760894 A JP 15760894A JP H0819372 A JPH0819372 A JP H0819372A
- Authority
- JP
- Japan
- Prior art keywords
- silk
- silk protein
- powder
- film
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 19
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 19
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 13
- 235000013305 food Nutrition 0.000 claims abstract description 9
- 241000255789 Bombyx mori Species 0.000 claims description 13
- 238000004108 freeze drying Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 108010022355 Fibroins Proteins 0.000 description 8
- 210000004907 gland Anatomy 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 210000003437 trachea Anatomy 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010013296 Sericins Proteins 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940088679 drug related substance Drugs 0.000 description 1
- 230000000075 effect on senility Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 210000002438 upper gastrointestinal tract Anatomy 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】この発明は、絹タンパク質の製造
方法に関するものである。さらに詳しくは、この発明
は、健康増進のために有益な飲食品添加物等として有用
な絹タンパク質の高効率で簡便な製造方法と、この方法
により得られた絹タンパク質に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing silk protein. More specifically, the present invention relates to a highly efficient and simple method for producing a silk protein useful as a food and drink additive useful for promoting health, and a silk protein obtained by this method.
【０００２】[0002]
【従来の技術とその課題】近年、コレステロールを低下
させ、肝臓を保護し、老人症に対しても効果がある等の
世評から、いわゆる健康増進の観点から絹タンパク質が
注目されている。この絹タンパク質については学術的に
もその有用性が検討されており（たとえば「化学」４７
巻８号、第５３０〜５３３頁）、絹糸の主成分であるフ
ィブロインは、分子量約３０万という大きな分子量を持
ち、成形可能なタンパク質として、食品、化粧品、医療
等のための素材として今後の技術展開に大きな期待が寄
せられているものである。2. Description of the Related Art In recent years, silk proteins have been attracting attention from the viewpoint of so-called health promotion from the public opinion that they lower cholesterol, protect the liver, and have an effect on senility. The usefulness of this silk protein has been studied academically (eg, “Chemistry” 47.
(Vol. 8, p. 530-533), fibroin, which is the main component of silk, has a large molecular weight of about 300,000, and is a future technology as a moldable protein and as a material for food, cosmetics, medical treatment, etc. There are great expectations for the development.
【０００３】このような絹タンパク質については、これ
までにもその製造方法が提案されており、たとえば蚕か
ら吐出された繭または生糸を酸またはアルカリ、もしく
は酵素処理して絹フィブロインを回収してさらに精製す
る方法（特開平２−１７７８６４号公報等）や、熟蚕か
ら絹糸線を摘出し、乾燥後に水で膨潤させ、水洗してセ
リシンを除去するフィブロインの製造方法（特開平３−
２０９３９９号公報）などが提示されている。For such silk protein, a method for producing such silk protein has been proposed so far. For example, cocoons or raw silk discharged from silkworms are treated with acid, alkali or enzyme to recover silk fibroin. A method for purification (Japanese Patent Application Laid-Open No. 2-177864, etc.), or a method for producing fibroin, in which a silk thread is extracted from a mature silkworm, dried, swollen with water, and washed with water to remove sericin (Japanese Patent Application Laid-Open No. 3-30078).
209399).
【０００４】しかしながら、従来の製造方法について
は、繭または生糸から絹フィブロインを取得する場合に
は、得られる絹フィブロインはＢ型の結晶構造を有して
いるため、これらを処理して食品用等に適したフィブロ
インのアミノ酸ポリマーまたはオリゴマーとして、ある
いは粉末として利用するにはその取扱いが面倒であっ
て、効率的ではなかった。However, according to the conventional production method, when silk fibroin is obtained from cocoons or raw silk, the obtained silk fibroin has a B-type crystal structure, and therefore, it is processed to be used for foods and the like. It was not efficient to use as an amino acid polymer or oligomer of fibroin suitable for the above or as a powder.
【０００５】また、絹糸線からフィブロインを製造する
従来の方法では、熟蚕の頭部の切断、腹部の押圧による
絹糸線の取出し等の面倒な手順が必要であり、同様に効
率的な手法であるとは言い難いのが実情であった。そこ
で、この発明は、以上の通りの従来技術の欠点を解消
し、取扱いが容易で、効率的な生産が可能とされる新し
い絹タンパク質の製造方法を提供することを目的として
いる。Further, in the conventional method for producing fibroin from a silk thread, a troublesome procedure such as cutting the head of a mature silkworm and removing the silk thread by pressing the abdomen is required, and the method is similarly efficient. It was hard to say that there was. Therefore, an object of the present invention is to solve the drawbacks of the conventional techniques as described above, to provide a new silk protein production method which is easy to handle and enables efficient production.
【０００６】[0006]
【課題を解決するための手段】この発明は、上記の課題
を解決するものとして、熟蚕を−３０℃以下で凍結乾燥
し、絹糸線を取出して粉末またはフィルム状とすること
を特徴とする絹タンパク質の製造方法を提供する。Means for Solving the Problems As a solution to the above problems, the present invention is characterized in that a mature silkworm is freeze-dried at -30 ° C or lower, and a silk thread is taken out to obtain a powder or a film. A method for producing silk protein is provided.
【０００７】[0007]
【作用】この発明は、熟蚕を−３０℃以下の温度におい
て凍結乾燥し、絹糸線を取出すことを大きな特徴として
いる。この際の凍結温度は極めて重要な条件であって、
−３０℃以下、特に−５０℃程度の凍結乾燥が、絹糸の
取出しにとっては重要である。−３０℃以上のたとえば
−２０℃での凍結処理では充分でない。The present invention is characterized in that the silkworm silk is removed by freeze-drying the silkworm at a temperature of -30 ° C or lower. The freezing temperature at this time is a very important condition,
Freeze-drying at −30 ° C. or lower, especially at −50 ° C. is important for taking out silk yarn. Freezing treatment at -30 ° C or higher, for example, at -20 ° C is not sufficient.
【０００８】−３０℃以下の温度での凍結乾燥により、
熟蚕の凍結体は容易に粉砕され、絹糸線が簡単に取出さ
れることになる。この絹糸線は、たとえば図１の解剖図
において、 ａ前腸 ｂ中腸中央縦走筋 ｃ気管叢 ｄ脂肪体 ｅ中
腸横走筋 ｆ中腸 ｇ卵巣 ｈ絹糸腺 ｉ小腸 ｊ結腸
ｋ直腸 ｌ気管灰白部 ｍ中腸縦走筋 ｎ縦走気管
ｏ精巣 ｐ周気管腺 ｑマルピーギ管 の（ｈ）絹糸線として示される部位にあり、拡大すると
図２のように、 ａ吐糸管 ｂフィリッピ腺 ｃ前部糸腺 ｄ中部糸腺
ｅ後部糸腺 によって構成されている。By lyophilization at temperatures below -30 ° C,
The frozen body of the mature silkworm is easily crushed, and the silk thread is easily taken out. This silk line is, for example, in the anatomical view of FIG. 1, a foregut b middle midgut longitudinal muscle c tracheal plexus d fat pad e midgut transverse muscle f midgut g ovary h silk gland i small intestine j colon k rectum l trachea Gray area m Midgut longitudinal muscle n Vertical trachea
o testis p peri-tracheal gland q malpighian canal (h) in a region shown as silk thread line, and when enlarged, as shown in Fig. 2, a spit tube b filippi gland c anterior thread gland d central thread gland
e It is composed of posterior thread glands.
【０００９】凍結乾燥には、−３０℃以下の温度を実現
するために、液体窒素、液体ヘリウム等の各種の冷媒が
使用される。凍結乾燥によって絹糸線タンパク質は容易
に粉末化され、かつ、この粉末によりフィルム状等に成
形することが容易となる。これらの粉末またはフィルム
は、分子量分布に応じて、あるいはその使用目的等に応
じて形状、大きさが選択される。そして、この粉末また
はフィルムは飲食品組成物、医療用組成物、化粧品等に
そのまま、あるいは適宜に加工して添加される。In freeze-drying, various refrigerants such as liquid nitrogen and liquid helium are used in order to achieve a temperature of -30 ° C or lower. By freeze-drying, the silk thread protein is easily pulverized, and the powder facilitates molding into a film or the like. The shape and size of these powders or films are selected depending on the molecular weight distribution or the purpose of use. Then, this powder or film is added to a food / beverage composition, a medical composition, a cosmetic or the like as it is or after being appropriately processed.
【００１０】さらにまた、これら粉末またはフィルム
は、膨潤、酵素分解、または加水分解し、フィブロイン
のアミノ酸ポリマー、オリゴマーとしても活用すること
が可能になる。フィルム状とするためには、各種のバイ
ンダー成分、たとえば他のタンパク質や多糖類等を用い
てもよい。なお、原料としての熟蚕は、目的とする絹糸
線タンパク質の食品、原薬等への利用を考慮すると、無
菌もしくは減菌飼育した熟蚕を用いるのがより好ましい
と考える。Furthermore, these powders or films can be swelled, enzymatically decomposed or hydrolyzed to be utilized as amino acid polymers and oligomers of fibroin. To form a film, various binder components such as other proteins and polysaccharides may be used. As the raw silkworm, it is more preferable to use a mature silkworm that has been sterilized or sterilized in consideration of the intended use of the silk thread protein in foods, drug substances and the like.
【００１１】以下、実施例を説明する。Examples will be described below.
【００１２】[0012]
【実施例】５歳熟蚕を液体窒素を用いて凍結乾燥した。
得られた蚕は、極めて簡単に砕け、絹糸線だけが回収さ
れた。この絹糸線を粉末とした。この粉末タンパク質
は、次の表１の通りのアミノ酸組成を有し、絹糸と近似
した組成のアミノ酸構成であることが確認された。Example A 5-year-old mature silkworm was freeze-dried using liquid nitrogen.
The obtained silkworm was crushed very easily and only the silk thread was recovered. This silk thread was used as a powder. It was confirmed that this powdery protein had an amino acid composition as shown in Table 1 below and had an amino acid composition similar to that of silk thread.
【００１３】この粉末はそのまま食品に添加して使用さ
れた。This powder was used as it was added to foods.
【００１４】[0014]
【表１】 [Table 1]
【００１５】[0015]
【発明の効果】以上詳しく説明した通り、この発明によ
り、極めて簡単に高効率での絹タンパク質の取得が可能
となり、得られた粉末またはフィルムはそのまま食品等
に利用することが可能となる。Industrial Applicability As described in detail above, according to the present invention, silk protein can be obtained very easily and highly efficiently, and the obtained powder or film can be used as it is for foods and the like.
【図１】絹糸線の部位を示した蚕の解剖図である。FIG. 1 is an anatomical view of a silkworm showing a portion of a silk thread.
【図２】絹糸線を拡大して示した図である。FIG. 2 is an enlarged view of a silk thread line.
Claims (4)
線を取出して粉末またはフィルム状とすることを特徴と
する絹タンパク質の製造方法。1. A method for producing a silk protein, which comprises freeze-drying a matured silkworm at −30 ° C. or lower and taking out a silk thread to obtain a powder or a film.
方法。2. The production method according to claim 1, which is freeze-dried at −50 ° C.
またはフィルム状の絹タンパク質。3. A powder or film silk protein produced by the method of claim 1.
品。4. A food or drink containing the silk protein of claim 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6157608A JP2763746B2 (en) | 1994-07-08 | 1994-07-08 | Method for producing silk protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6157608A JP2763746B2 (en) | 1994-07-08 | 1994-07-08 | Method for producing silk protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0819372A true JPH0819372A (en) | 1996-01-23 |
| JP2763746B2 JP2763746B2 (en) | 1998-06-11 |
Family
ID=15653451
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6157608A Expired - Fee Related JP2763746B2 (en) | 1994-07-08 | 1994-07-08 | Method for producing silk protein |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2763746B2 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002262792A (en) * | 2001-03-09 | 2002-09-17 | Ryoso:Kk | Low-protein artificial rice for therapeutic diet and method for producing the same |
| US6482420B2 (en) | 2000-12-27 | 2002-11-19 | Noboru Huziwara | Composition having bactericidal action, cosmetics containing said composition and ultraviolet ray screening agent |
| CN105850901A (en) * | 2016-04-18 | 2016-08-17 | 华南农业大学 | Detection of concentration of ammonia in breeding environment and application thereof in establishing silkworm growth and development judgment system |
| CN107440085A (en) * | 2017-07-11 | 2017-12-08 | 四川省蚕业科技开发总公司 | A kind of production technology of edible silkworm chrysalis |
| JPWO2021251387A1 (en) * | 2020-06-09 | 2021-12-16 |
-
1994
- 1994-07-08 JP JP6157608A patent/JP2763746B2/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6482420B2 (en) | 2000-12-27 | 2002-11-19 | Noboru Huziwara | Composition having bactericidal action, cosmetics containing said composition and ultraviolet ray screening agent |
| JP2002262792A (en) * | 2001-03-09 | 2002-09-17 | Ryoso:Kk | Low-protein artificial rice for therapeutic diet and method for producing the same |
| CN105850901A (en) * | 2016-04-18 | 2016-08-17 | 华南农业大学 | Detection of concentration of ammonia in breeding environment and application thereof in establishing silkworm growth and development judgment system |
| CN107440085A (en) * | 2017-07-11 | 2017-12-08 | 四川省蚕业科技开发总公司 | A kind of production technology of edible silkworm chrysalis |
| JPWO2021251387A1 (en) * | 2020-06-09 | 2021-12-16 | ||
| WO2021251387A1 (en) * | 2020-06-09 | 2021-12-16 | Ｓｐｉｂｅｒ株式会社 | Meat-like food composition |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2763746B2 (en) | 1998-06-11 |
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