JPH082297B2 - アルギン酸分解酵素及びその製造法と使用菌並びにアルギン酸分解法 - Google Patents

アルギン酸分解酵素及びその製造法と使用菌並びにアルギン酸分解法

Info

Publication number: JPH082297B2
Authority: JP; Japan
Prior art keywords: alginate; enzyme; degrading enzyme; optimum; alginic acid
Prior art date: 1989-09-07
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

JP1230511A

Other languages

English (en)

Japanese (ja)

Other versions

JPH0394675A (ja

Inventor

克巳村田

和雄村上

功日下部

稔秀佐藤

Original Assignee

株式会社紀文

株式会社紀文フードケミファ

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-09-07

Filing date

1989-09-07

Publication date

1996-01-17

1989-09-07 Application filed by 株式会社紀文, 株式会社紀文フードケミファ filed Critical 株式会社紀文

1989-09-07 Priority to JP1230511A priority Critical patent/JPH082297B2/ja

1989-12-13 Priority to CA002005398A priority patent/CA2005398A1/fr

1991-04-19 Publication of JPH0394675A publication Critical patent/JPH0394675A/ja

1993-10-06 Priority to US08/132,223 priority patent/US5348875A/en

1996-01-17 Publication of JPH082297B2 publication Critical patent/JPH082297B2/ja

2011-01-17 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

102000004190 Enzymes Human genes 0.000 title claims description 63
108090000790 Enzymes Proteins 0.000 title claims description 63
241000894006 Bacteria Species 0.000 title claims description 14
238000004519 manufacturing process Methods 0.000 title claims description 13
238000000034 method Methods 0.000 title claims description 7
235000010443 alginic acid Nutrition 0.000 claims description 21
229920000615 alginic acid Polymers 0.000 claims description 21
IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 12
229960001126 alginic acid Drugs 0.000 claims description 12
239000000783 alginic acid Substances 0.000 claims description 12
150000004781 alginic acids Chemical class 0.000 claims description 12
239000000661 sodium alginate Substances 0.000 claims description 12
235000010413 sodium alginate Nutrition 0.000 claims description 12
229940005550 sodium alginate Drugs 0.000 claims description 12
239000002253 acid Substances 0.000 claims description 10
FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims description 9
229940072056 alginate Drugs 0.000 claims description 9
241000588914 Enterobacter Species 0.000 claims description 8
238000000354 decomposition reaction Methods 0.000 claims description 4
229920001542 oligosaccharide Polymers 0.000 claims description 4
150000002482 oligosaccharides Chemical class 0.000 claims description 4
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims description 3
239000000758 substrate Substances 0.000 claims description 3
238000012258 culturing Methods 0.000 claims description 2
241000822976 bacterium M-1 Species 0.000 claims 1
244000005700 microbiome Species 0.000 claims 1
239000000243 solution Substances 0.000 description 35
230000000694 effects Effects 0.000 description 14
238000006243 chemical reaction Methods 0.000 description 13
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 9
239000000872 buffer Substances 0.000 description 8
239000006228 supernatant Substances 0.000 description 8
238000005194 fractionation Methods 0.000 description 7
238000011084 recovery Methods 0.000 description 7
RVBUGGBMJDPOST-UHFFFAOYSA-N 2-thiobarbituric acid Chemical compound O=C1CC(=O)NC(=S)N1 RVBUGGBMJDPOST-UHFFFAOYSA-N 0.000 description 6
230000009471 action Effects 0.000 description 6
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 6
238000006911 enzymatic reaction Methods 0.000 description 6
238000000746 purification Methods 0.000 description 6
229920005654 Sephadex Polymers 0.000 description 5
239000012507 Sephadex™ Substances 0.000 description 5
150000003839 salts Chemical class 0.000 description 5
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
238000002835 absorbance Methods 0.000 description 4
229910052921 ammonium sulfate Inorganic materials 0.000 description 4
235000011130 ammonium sulphate Nutrition 0.000 description 4
150000001875 compounds Chemical class 0.000 description 4
238000010586 diagram Methods 0.000 description 4
239000002609 medium Substances 0.000 description 4
239000000203 mixture Substances 0.000 description 4
239000000047 product Substances 0.000 description 4
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
102000004317 Lyases Human genes 0.000 description 3
108090000856 Lyases Proteins 0.000 description 3
230000008859 change Effects 0.000 description 3
230000000593 degrading effect Effects 0.000 description 3
229910052751 metal Inorganic materials 0.000 description 3
239000002184 metal Substances 0.000 description 3
239000011734 sodium Substances 0.000 description 3
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical group CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
239000001888 Peptone Substances 0.000 description 2
108010080698 Peptones Proteins 0.000 description 2
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
238000005377 adsorption chromatography Methods 0.000 description 2
229910000147 aluminium phosphate Inorganic materials 0.000 description 2
239000011575 calcium Substances 0.000 description 2
229910052791 calcium Inorganic materials 0.000 description 2
229940041514 candida albicans extract Drugs 0.000 description 2
230000003247 decreasing effect Effects 0.000 description 2
238000010828 elution Methods 0.000 description 2
239000000499 gel Substances 0.000 description 2
239000001963 growth medium Substances 0.000 description 2
229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
235000019796 monopotassium phosphate Nutrition 0.000 description 2
229910052757 nitrogen Inorganic materials 0.000 description 2
235000019319 peptone Nutrition 0.000 description 2
239000011591 potassium Substances 0.000 description 2
229910052700 potassium Inorganic materials 0.000 description 2
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
239000011541 reaction mixture Substances 0.000 description 2
102220201851 rs143406017 Human genes 0.000 description 2
238000004809 thin layer chromatography Methods 0.000 description 2
239000012138 yeast extract Substances 0.000 description 2
AYRXSINWFIIFAE-UHFFFAOYSA-N 2,3,4,5-tetrahydroxy-6-[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexanal Chemical compound OCC1OC(OCC(O)C(O)C(O)C(O)C=O)C(O)C(O)C1O AYRXSINWFIIFAE-UHFFFAOYSA-N 0.000 description 1
PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
KUWPCJHYPSUOFW-YBXAARCKSA-N 2-nitrophenyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1[N+]([O-])=O KUWPCJHYPSUOFW-YBXAARCKSA-N 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
102000016938 Catalase Human genes 0.000 description 1
108010053835 Catalase Proteins 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
241000588697 Enterobacter cloacae Species 0.000 description 1
241000589565 Flavobacterium Species 0.000 description 1
108010010803 Gelatin Proteins 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
IAJILQKETJEXLJ-SQOUGZDYSA-N L-guluronic acid Chemical compound O=C[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O IAJILQKETJEXLJ-SQOUGZDYSA-N 0.000 description 1
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
-1 Lactose enol Chemical class 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
229930195725 Mannitol Natural products 0.000 description 1
229910002651 NO3 Inorganic materials 0.000 description 1
AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
102000004316 Oxidoreductases Human genes 0.000 description 1
108090000854 Oxidoreductases Proteins 0.000 description 1
241000589774 Pseudomonas sp. Species 0.000 description 1
241000607284 Vibrio sp. Species 0.000 description 1
230000004913 activation Effects 0.000 description 1
238000005273 aeration Methods 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
AEMOLEFTQBMNLQ-UHFFFAOYSA-N beta-D-galactopyranuronic acid Natural products OC1OC(C(O)=O)C(O)C(O)C1O AEMOLEFTQBMNLQ-UHFFFAOYSA-N 0.000 description 1
229910052793 cadmium Inorganic materials 0.000 description 1
BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
239000012295 chemical reaction liquid Substances 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
238000006482 condensation reaction Methods 0.000 description 1
239000012228 culture supernatant Substances 0.000 description 1
AEMOLEFTQBMNLQ-YBSDWZGDSA-N d-mannuronic acid Chemical compound O[C@@H]1O[C@@H](C(O)=O)[C@H](O)[C@@H](O)[C@H]1O AEMOLEFTQBMNLQ-YBSDWZGDSA-N 0.000 description 1
239000007857 degradation product Substances 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
238000000605 extraction Methods 0.000 description 1
238000001914 filtration Methods 0.000 description 1
239000008273 gelatin Substances 0.000 description 1
229920000159 gelatin Polymers 0.000 description 1
235000019322 gelatine Nutrition 0.000 description 1
235000011852 gelatine desserts Nutrition 0.000 description 1
238000002523 gelfiltration Methods 0.000 description 1
239000008103 glucose Substances 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
229910052588 hydroxylapatite Inorganic materials 0.000 description 1
PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
239000002054 inoculum Substances 0.000 description 1
238000004255 ion exchange chromatography Methods 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
239000008101 lactose Substances 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
239000000594 mannitol Substances 0.000 description 1
235000010355 mannitol Nutrition 0.000 description 1
238000005259 measurement Methods 0.000 description 1
239000013028 medium composition Substances 0.000 description 1
229910021645 metal ion Inorganic materials 0.000 description 1
230000004899 motility Effects 0.000 description 1
229960003104 ornithine Drugs 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
230000000704 physical effect Effects 0.000 description 1
229910000160 potassium phosphate Inorganic materials 0.000 description 1
239000008057 potassium phosphate buffer Substances 0.000 description 1
235000011009 potassium phosphates Nutrition 0.000 description 1
230000009467 reduction Effects 0.000 description 1
238000011218 seed culture Methods 0.000 description 1
PTLRDCMBXHILCL-UHFFFAOYSA-M sodium arsenite Chemical compound [Na+].[O-][As]=O PTLRDCMBXHILCL-UHFFFAOYSA-M 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
239000002689 soil Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
239000000600 sorbitol Substances 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
230000019086 sulfide ion homeostasis Effects 0.000 description 1
239000001052 yellow pigment Substances 0.000 description 1

Classifications

- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

JP1230511A 1989-09-07 1989-09-07 アルギン酸分解酵素及びその製造法と使用菌並びにアルギン酸分解法 Expired - Fee Related JPH082297B2 (ja)

Priority Applications (3)

Application Number	Priority Date	Filing Date	Title
JP1230511A JPH082297B2 (ja)	1989-09-07	1989-09-07	アルギン酸分解酵素及びその製造法と使用菌並びにアルギン酸分解法
CA002005398A CA2005398A1 (fr)	1989-09-07	1989-12-13	Alginase et sa preparation; bacteries utilisees et methode de decomposition de l'acide alginique
US08/132,223 US5348875A (en)	1989-09-07	1993-10-06	Production of alginase from Enterobacter cloacae M-1 (FERM BP-2577)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP1230511A JPH082297B2 (ja)	1989-09-07	1989-09-07	アルギン酸分解酵素及びその製造法と使用菌並びにアルギン酸分解法

Publications (2)

Publication Number	Publication Date
JPH0394675A JPH0394675A (ja)	1991-04-19
JPH082297B2 true JPH082297B2 (ja)	1996-01-17

Family

ID=16908901

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP1230511A Expired - Fee Related JPH082297B2 (ja)	1989-09-07	1989-09-07	アルギン酸分解酵素及びその製造法と使用菌並びにアルギン酸分解法

Country Status (2)

Country	Link
JP (1)	JPH082297B2 (fr)
CA (1)	CA2005398A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
KR101239757B1 (ko) *	2012-11-15	2013-03-06	주식회사 지디	알긴산 분해효소를 생산하는 신규 미생물 페니바실러스 라우투스 ｇｄ－ａ2와 알긴산 분해용 생촉매 및 이를 이용한 알긴산 올리고당의 제조 방법

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US8283338B2 (en)	2007-11-30	2012-10-09	Kao Corporation	GIP secretion inhibitor

1989
- 1989-09-07 JP JP1230511A patent/JPH082297B2/ja not_active Expired - Fee Related
- 1989-12-13 CA CA002005398A patent/CA2005398A1/fr not_active Abandoned

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
KR101239757B1 (ko) *	2012-11-15	2013-03-06	주식회사 지디	알긴산 분해효소를 생산하는 신규 미생물 페니바실러스 라우투스 ｇｄ－ａ2와 알긴산 분해용 생촉매 및 이를 이용한 알긴산 올리고당의 제조 방법

Also Published As

Publication number	Publication date
CA2005398A1 (fr)	1991-03-07
JPH0394675A (ja)	1991-04-19

Legal Events

Date	Code	Title	Description
2005-02-22	R250	Receipt of annual fees	Free format text: JAPANESE INTERMEDIATE CODE: R250
2005-12-06	R250	Receipt of annual fees	Free format text: JAPANESE INTERMEDIATE CODE: R250
2006-11-28	R250	Receipt of annual fees	Free format text: JAPANESE INTERMEDIATE CODE: R250
2007-11-29	FPAY	Renewal fee payment (event date is renewal date of database)	Free format text: PAYMENT UNTIL: 20080117 Year of fee payment: 12
2007-12-04	FPAY	Renewal fee payment (event date is renewal date of database)	Free format text: PAYMENT UNTIL: 20090117 Year of fee payment: 13
2009-01-17	LAPS	Cancellation because of no payment of annual fees

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US5348875A (en)	1994-09-20	Production of alginase from Enterobacter cloacae M-1 (FERM BP-2577)
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JPH0614772A (ja)	1994-01-25	新規エステル分解酵素ａおよびその製造方法
EP0320685B1 (fr)	1993-10-13	Procédé de préparation d'un enzyme alpha amylase thermostable à l'aide d'une culture de micro-organimes superconducteurs
JPH10229876A (ja)	1998-09-02	α−１，３−多分岐デキストラン水解酵素、その製造法及び環状イソマルトオリゴ糖の製造法
JP2843110B2 (ja)	1999-01-06	プルラナーゼ
JPH08280395A (ja)	1996-10-29	トレハロースの製造方法

JPH082297B2 - アルギン酸分解酵素及びその製造法と使用菌並びにアルギン酸分解法 - Google Patents

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