JPH08291012A - Soil disease control method for plants - Google Patents

Abstract

(57) [Abstract] [Purpose] A method for controlling soil diseases of plants by applying a fungus belonging to the genus Gliocladium to the plant cultivation soil, wherein Provided is a method for controlling soil disease of a cultivated plant efficiently and persistently by enhancing the growth property, the fixing property and the storage property. [Composition] In a method for controlling soil diseases of plants by applying a fungus belonging to the genus Gliocladium to the soil for cultivating plants, organic matter, preferably peat moss, wood chips, pulp dregs, matured leaf soil, to the soil for cultivating plants, The fully-ripened compost is mixed so that the dry weight is 5% by weight or more based on the total amount of the soil.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plant soil disease control method, and more particularly to an effective plant soil disease control method using a fungus belonging to the genus Gliocladium.

[0002]

2. Description of the Related Art In recent years, as a method for protecting cultivated plants from various diseases, a method of preventing the occurrence of diseases by using microorganisms that compete with pathogenic bacteria causing various diseases in consideration of safety and sustainability of effects has been proposed. Widely used.

Microorganisms used to control soil diseases of cultivated plants so far include Trichoderma spp.
Examples include fungi belonging to the genus Gliocladium, actinomycetes belonging to the genus Streptomyces, bacteria belonging to the genus Bacillus and Pseudomonas, and the method for controlling soil diseases of plants using these microorganisms and containing these microorganisms. A large number of soil disease control agents that are used are also being researched and developed.

Among these, as research and development relating to soil disease control using a fungus belonging to the genus Gliocladium, for example, a porous carrier and a sporulated dilute acid described in JP-A-3-95105. And a biocontrol preparation for suppressing or preventing soil diseases of plants containing fungi (Trichoderma spp., Talalomyces spp., Gliocladium spp., Etc.) treated with the above, and JP-A-5-262.
By culturing a fungus (Trichoderma spp., Gliocladium spp.) Having the ability to control a soil-based plant pathogen described in Japanese Patent No. 614, and exposing this culture to an inactivated soil-based plant pathogen. There is a method for producing a soil disease controlling agent, which comprises increasing the control ability of the fungi against soil plant pathogens to obtain a soil disease controlling agent.

When the fungus belonging to the genus Gliocladium is used for soil disease control as described above, the bacterium belonging to the genus Gliocladium is usually used as a cell culture. As a method for culturing bacterial cells, a method of culturing using a liquid medium such as potato dextrose medium, Tuapec Dox medium, or Sabouraud medium is generally used, but the liquid culture is poor in preservation and the bacterial cells at the end of the culture are cultivated. Had a problem of rapid death and lysis. Therefore, as a method for improving the preservation property of the culture, as in the invention described in JP-A-3-95105, a method of storing the sporulated cell culture by mixing it with a porous carrier, or A method has been adopted in which a porous carrier is impregnated with the liquid medium and cultured as a bacterial cell culture in which many spores are formed on the carrier. But,
Even with a microbial cell-containing composition having improved storability of such microbial cells, when it is applied to a plant-cultivating soil, a nutrient source necessary for the microbial cells to grow in the plant-cultivating soil is sufficient. If not present, there was a problem that fungi belonging to the genus Gliocladium did not grow in the soil for plant cultivation, or did not grow slowly and had poor fixability, and soil diseases could not be controlled sufficiently.

As described above, in a method for controlling soil diseases of plants by applying fungi belonging to the genus Gliocladium to the soil for cultivating plants, in the soil for cultivation after applying the fungus belonging to the genus Gliocladium. There has been a demand for a soil disease control method for proliferating and colonizing fungi belonging to the genus Gliocladium and improving the preservability to efficiently and sustainably control soil diseases of cultivated plants.

[0007]

The present invention has been made from the above viewpoint, and a method for controlling soil diseases of plants by applying a fungus belonging to the genus Gliocladium to the soil for plant cultivation, It is an object of the present invention to provide a method for efficiently and sustainably controlling soil diseases of cultivated plants by enhancing the growth, colonization and storability of fungi belonging to the genus Gliocladium in cultivation soil.

[0008]

In order to solve the above-mentioned problems, the present inventors blended organic matter in plant-cultivating soil in an amount of 5% by weight or more on a dry weight basis based on the total amount of the soil, and then adding gliocladium thereto. It was decided to apply fungi belonging to the genus.

That is, the present invention relates to a method for controlling soil diseases of plants by applying a fungus belonging to the genus Gliocladium to the soil for cultivating plants, wherein the organic soil is added to the soil for cultivating plants in a dry weight with respect to the total amount of the soil. It is a method for controlling soil diseases of plants, which is characterized in that the content is 5% by weight or more.

Hereinafter, the present invention will be described in detail. In the soil disease control method of the present invention, to control the soil disease of the plant by applying a fungus belonging to the genus Gliocladium to the soil for plant cultivation, as the Gliocladium genus used in the present invention, It is not particularly limited as long as it is a fungus belonging to the genus Gliocladium, and examples thereof include Gliocladium aureum and Gliocladium catenulatu.
m), Gliocladium delicate essence (Gliocla
dium deliquescens), Gliocladium nigrum, Gliocladium penicilloides, Gliocladium roseum, Gliocladium sagariensi
s), Gliocladium vermoeseni
vermoeseni), Glioocdium virens (Glioc
ladium virens) and the like. They are,
One kind may be used alone, or two or more kinds may be mixed and used.

When the fungus belonging to the genus Gliocladium is used in the present invention, a culture obtained by culturing the cells in a medium in which the cells of the fungus belonging to the genus Gliocladium can grow can be used. It is more preferable to use a culture containing a fungus belonging to the genus Gliocladium in which spores are sufficiently formed by culture.

Cultivation of a fungus belonging to the genus Gliocladium can be carried out in the same manner as a usual culture method for microorganisms. For example, in the laboratory, a culture method such as culturing in a potato dextrose agar medium at 25 ° C. for 10 days can be mentioned. In the case of large-scale culture, ordinary liquid culture or solid culture using a plant-derived solid component such as bran, a porous body impregnated with sugar or a nitrogen source, or the like is possible.

Further, in order to obtain a culture of the genus Gliocladium that has been sufficiently sporulated, for example, the genus Gliocladium may be cultured by the following method. First, a gliocladium genus is liquid-cultured in a potato dextrose medium sterilized by an autoclave or the like. Next, as a medium, plant-derived solid components, for example, corn residue, sweet potato, potato skin and residue after starch separation, vegetables, fruit pomace, stevia extract residue, beer dregs, sake lees (yeast Solids, etc. obtained from cereals, beans, oil seeds, etc., and porous carriers such as zeolite, attapulgite, sepialite, montmorillonite, perlite, Kanuma soil, red jade stone, pumice, charcoal, coral sand, etc. Prepare what is contained (preferable mixing ratio is 5 to 70% by weight of the amount of the porous carrier compounded with the amount of the plant-derived solid component), sterilize it with an autoclave, etc. Inoculate a liquid culture of Umbus and incubate at 15 to 35 ° C. for 2 to 40 days. The obtained culture can be used in the present invention as it is or after being dried if necessary.

In the method for controlling soil diseases of the present invention, the fungal culture belonging to the genus Gliocladium thus prepared is applied to the soil for cultivating plants. Organic matter in cultivation soil as a nutrient source,
It is blended so that when the whole soil is dried, 5% by weight or more of organic matter is contained with respect to the total amount of the soil. When applying a fungal culture belonging to the genus Gliocladium, if the organic matter content in the cultivation soil is 5% by weight or less, the ability of the fungus belonging to the genus Gliocladium to establish in soil, proliferative property, and storability May decrease, and a sufficient soil disease control effect may not be obtained.

In the control method of the present invention, the organic matter to be contained in the cultivation soil is not particularly limited as long as it is an organic matter. For example, cereals, beans, and wastes thereof (brass, rice bran, okara, etc.), Examples include ripe compost, ripe leaf soil, pulp dregs, peat moss, wood chips, organic substances contained in bark of cedar and the like. Further, among these organic substances, in the present invention, it is preferable to use an organic substance that can be a nutrient source for Gliocladium spp., Although it is difficult to be a nutrient source for various filamentous fungi and bacteria generally existing in soil. Used. As the organic matter preferably used in the soil disease control method of the present invention having such characteristics, organic matter that is advanced in fermentation contained in ripe compost and ripe mulch, pulp pulp, peat moss,
Examples include organic substances that are not easily decomposed by general soil microorganisms contained in wood chips, bark of cedar and the like. In the present invention, by adding such an organic matter to the cultivated soil, it is possible to selectively grow only the fungus belonging to the genus Gliocladium to settle in the soil, and also improve the preservability of the bacterium. Therefore, it is possible to control soil diseases efficiently and sustainably.

When an organic matter is contained in the soil for cultivation in the method for controlling soil diseases of the present invention, the organic matter may be taken out from various plant-derived or animal-derived materials and used, but the organic matter-containing substance is included from the viewpoint of usability. It is preferable to use the material itself. Examples of plant-derived organic matter-containing materials include pulp dregs, peat moss, wood chips, bark of cedar, and the like, and animal-derived organic matter-containing materials include cow dung, chicken dung, and fish meal. . Further, these plant-derived and animal-derived various organic material-containing materials can be used alone or as a mixture of two or more kinds, but as a mixture of two or more kinds, ripe compost, ripe mulch, etc. Can be preferably mentioned. Furthermore, among these, in the present invention, it is preferable to use a material mainly composed of a plant-derived organic matter-containing material, for example, ripe mulch, ripe compost, peat moss, pulp dregs, wood chips and the like. These organic-substance-containing materials contain organic substances in a proportion of approximately 5 to 95% by weight, although they vary depending on the materials.

In the present invention, these organic material-containing materials are blended so that the organic material content is 5% by weight or more based on the total amount of soil for cultivation. Most of the organic material-containing materials derived from plants are in soil. Since it rarely contains organic matter that ferments with oxygen and consumes oxygen or generates a large amount of harmful substances such as ammonia and hydrogen sulfide, when mixing plant-derived organic matter-containing material with the soil, It is also possible to set the upper limit of the compounding amount of the organic substance-containing material to 100% by weight. However, animal-derived organic matter-containing materials often contain organic matter that ferments in the soil and consumes oxygen and generates a large amount of harmful substances such as ammonia and hydrogen sulfide. When mixed with the soil, the upper limit of the amount of the animal-derived organic material-containing material to be mixed with respect to the total soil is preferably about 30% by weight.

Here, as the ripe mulch used for controlling soil diseases of the present invention, it is preferable to use a well-decomposed ripe mulch obtained from a deciduous forest zone, but such a mature mulch becomes gradually difficult to obtain. As a substitute therefor, it is possible to use, for example, a fully-ripened mulch produced as follows. In the maturation area with a roof, deciduous leaves of deciduous trees centering on rice husk, cherry tree, and oak,
Activated sludge with a water content of about 60%, Okara with a water content of about 65%,
Stack chicken dung, chemical fertilizer, etc. in appropriate amounts in layers and
While cutting back at a rate of about once every four months, ripen for half a year to two years to make a fully-ripened leaf soil.

Further, the fully-ripened compost used for controlling soil diseases of the present invention is no different from what is generally called fully-ripened compost, and can be produced, for example, as follows. Appropriate amount of rice straw, sawdust, cow dung, coal ash, etc. are piled up on a maturing station with a roof, cut back at a rate of about once every 2-3 months, and matured for half a year to 2 years to make a fully-ripened compost. The fully-ripened leaf soil and the fully-ripened compost thus obtained are preferably used in the present invention in which the fermentation is sufficiently advanced for the above reasons.

When peat moss is used as the organic matter-containing material, humic acid or the like contained in peat moss may dissolve in water to lower the pH of the cultivated soil and may damage cultivated plants. It is preferable to adjust the pH of the peat moss by washing with water or by adding calcium oxide, calcium carbonate, coal ash, charcoal or the like to the peat moss before blending with.

As the base material of the soil for cultivation in the method for controlling soil diseases of the present invention, general materials such as upland soil, rice soil,
One or more selected from mountain soil, Akadama soil, charcoal, zeolite, perlite, vermiculite, Kanuma soil, pumice stone, coral sand and the like can be mentioned. In addition to the above organic substances, it is possible to add various components to the cultivation soil according to various purposes. For example, slow-release fertilizers are often added.

A fungus belonging to the genus Gliocladium (such as a culture of the above-mentioned Gliocladium genus) is applied to the thus-prepared cultivation soil at the time of plant cultivation. The seeds of the cultivated plant are sprinkled with Gliocladium spp. And sown on the soil for cultivation. When acclimatizing melicron bacteria, Glyocladium spp. Is added to the soil for acclimatization. Cultivate plants by applying Umbu bacteria, for example, sowing seeds of cultivated plants, inoculate, sow tanemo, or make seedlings in pots, or when planting Gliocladium spp. Examples thereof include a method of applying the soil to the root of the plant and a method of mixing Gliocladium spp. To the surface soil around the roots of the transplanted seedlings.

Application of fungi belonging to the genus Gliocladium
Regarding the amount, glioclade is used for 100 g of cultivation soil.
10-10 as colony forming unit of the genus¹¹CFU
It is preferable to apply it so that This application rate
Soil with less than 10 CFU per 100 g of cultivation soil
The expression of disease control effect tends to be unstable, and ¹¹
Even if it is applied over CFU, the effect will reach the ceiling and it will be economical.
It is a disadvantage.

The plant to which the soil disease control method of the present invention is applied is not particularly limited, but a vegetable selected from the solanaceae such as eggplant, cucumber, spinach, leeks, etc., Cucurbitaceae, Acalyptaceae and Lilyaceae is used in the present invention. The plant can be mentioned as a plant that exhibits a remarkable effect when the soil disease control method is applied. Further, as soil diseases of plants to which the soil disease control method of the present invention is applied, those in which the pathogen belongs to fungi, for example, Pythium, Rhizoctonia, Corticium, Sclerotinia, Verticillium, Fusarium. Alternatively, a soil disease in which a fungus belonging to the genus Phytophytra is used as a pathogen can be mentioned.

[0025] Specifically, for example, Rhizoctonia solani, such as eggplant, tomato, pepper, cabbage, etc., and white silkworm, Corticium rolfushie, such as leek, chrysanthemum, green bean, etc.
sii), lettuce, cabbage, and other sclerotinia sclerotiorum (Sclerotinia sclerotiorum), spinach seedling wilt disease Pythium ultimum (Pyth
ium ultimum) and Pythium afanidelmartum (Pyt
hium aphanidermatum), eggplant, tomato etc. Verticillium dahlier (Verticillium dahrie)
liae), wilt fungus such as tomato and spinach, Fusarium wilt disease such as cabbage and turnip, Fusarium oxysporum, Phytophthora capsichi (Phytophthora capsi)
ci) etc. can be mentioned.

[0026]

Embodiments of the present invention will be described below.

[0027]

[Production Example 1] Cultivation of fungi belonging to the genus Gliocladium An inorganic carrier obtained by mixing 1 part by weight of zeolite with 1 part by weight of calcined attapulgite having a particle size of 2 to 3 mm and potato dextrose medium at a rate of 350 mL per 1 kg Was placed in a stainless steel vat with a lid of 25 cm × 30 cm × 10 cm and sterilized by autoclave at 120 ° C. for 20 minutes to obtain a solid medium.

Gliocladium virens IFO 916 was added to the solid medium obtained above.
9 was inoculated and cultured at 28 ° C. for 14 days, 5 L of sterilized water was added, gently stirred, and filtered with a sieve having a diameter of 0.2 mm. The obtained filtrate is centrifuged to precipitate a solid, and the taken-out precipitate is suspended in sterilized water to give 7.1 × 1.
A fungal suspension belonging to the genus Gliocladium containing ⁰⁹ spores / mL was obtained.

Further, in the same manner as the above-mentioned Gliocladium virens, a fungal suspension belonging to the genus Gliocladium containing 4.3 × 10 ⁹ spores of Gliocladium deliquescens IFO9016 / mL. A suspension was obtained.

[0030]

[Production Example 2] Cultivation of fungi belonging to the genus Gliocladium Tapa water was added to make the water content 67%, and 3 g of bran 25c
1 kg each was put into three stainless steel bats with a lid of m × 30 cm × 10 cm, and sterilized at 120 ° C. for 20 minutes in an autoclave. After that, each stainless bat was placed on the Gliocladium deli essence.
quescens) IFO 9016, Gliocladium penicilloides IFO5
869, Gliocladium
roseum) IFO31390 was inoculated and cultured at 27 ° C. for 18 days.

[0031]

[Production Example 3] Preparation of pathogen contamination source An inorganic carrier obtained by mixing 1 part by weight of baked montmorillonite having a particle size of 1 to 3 mm, baked zeolite, and baked attapulgite is impregnated with 400 mL of potato dextrose medium per kg. It was 60% water content
1 kg of bran is added and mixed, 40 cm x 60 cm x
It was placed in a stainless steel bat with a 10 cm lid. A total of four stainless steel vats containing such a medium were prepared, sterilized in an autoclave at 120 ° C. for 20 minutes, and then the stainless steel vats were each subjected to Rhizoctonia solani (Rhizoctonia).
solani), Corticium
rolfsii), Pythium ultimu
m), Pythium aphan
idermetum) pathogen. The four stainless steel vats were placed in an incubator at 24 ° C. and cultured for 16 days. Then, the stainless vat was transferred to a ventilation dryer at 27 ° C., and the contents were dried for 2 days with stirring to prepare a pathogen inoculum.

[0032]

[Examples 1 to 4] 60 kg of red tama soil baked at 800 ° C,
To 20 kg of pumice stone and 20 kg of peat moss adjusted to pH 6.2 with calcium oxide, 250 g of slow-acting fertilizer Magamp K (manufactured by Grace) was added to prepare a cultivation soil. The organic matter content of this cultivation soil was 14.6% in terms of dry matter. This cultivation soil was put into each of four plastic containers of 36 cm × 45 cm × 15 cm from the bottom to 10 cm, and 300 mL of the spore suspension of gliocladium virens produced in Production Example 1 was sprinkled on each of them and mixed well. Watered well. After placing these 4 containers in a greenhouse and lightly irrigating them once a day for 4 days, 3 types of pathogen inoculum prepared in Production Example 3 (Pisium ultimum, Rhizoctonia solani (container Two
1) and Pythium afanidelmartum) 1 each
50 g of each was sprayed, and only the surface layer was lightly mixed.

Then, 30 seeds of spinach (variety: okame) were placed in a container inoculated with Pythium ultimum and a container inoculated with Pythium afanidelmartum (Examples 1 and 2) and Rhizoctonia solani. 30 seeds of cucumber (cultivar: Tokiwa Crawling) were placed in one of the containers inoculated with (Example 3), and eggplant (cultivar: in the other one of the containers inoculated with Rhizoctonia solani).
Thirty seeds of Senryo No. 2) were sown (Example 4). These containers were placed in a greenhouse, seedlings were raised for 30 days, and the number of dead seedlings was measured.

For comparison, cultivation soil was prepared in the same manner as in Examples 1 to 4 except that 20 kg of vermiculite was used instead of 20 kg of peat moss.
The organic matter content of this cultivation soil was 1% or less in terms of dry matter. The same test as in Examples 1 to 4 was performed using this cultivation soil.

The test results of the above examples are shown in Table 1 together with the test results of the comparative examples.

[0036]

[Table 1]

As is clear from these results, according to the soil disease control method of the present invention in which the organic matter is contained in the plant cultivation soil and the fungus belonging to the genus Gliocladium is inoculated, the plant cultivation soil containing almost no organic matter. Compared to the comparative example in which the fungus belonging to the genus Gliocladium was inoculated, cucumber,
It controls soil diseases of eggplant and spinach very efficiently.

[0038]

[Production Example 4] Preparation of nursery soil Soil was sifted to remove particles of 3 mm or more. This mountain soil 200kg, perlite 40kg, natural zeolite 20
kg, vermiculite 10 kg, coral sand 5 kg and slow-release fertilizer magamp K 400 g were added. The total amount of organic matter in this nursery soil was 1% or less in terms of dry matter.

[0039]

[Production Example 5] Preparation of fully-ripened leaf soil To 10 m ³ of deciduous leaves of deciduous trees centered on rice husk 3 m ³ , cherry tree and oak, 80 kg of activated sludge having a water content of 60%, 120 kg of okara at a water content of 65%, 90 kg of poultry manure, chemical fertilizer (N- (P−K = 10−10−10) 32 kg was piled up in layers on a maturing plant with a roof and ripened for 1.5 years while turning back once every 4 months. The organic matter content of this ripe mulch was 21.8% in terms of dry matter.

[0040]

[Production Example 6] Preparation of fully-ripened compost Rice straw 100 kg, sawdust 200 kg, cow dung 5000 k
g, 500 kg of coal ash is piled up on a maturing plant with a roof,
It was cut back once every three months and aged for 1.5 years to produce a fully-ripened compost. The organic matter content of this ripe compost is 17.
It was 2%.

[0041]

[Examples 5 to 9] Production Example 5 was applied to the nursery soil produced in Production Example 4.
After mixing the ripe mulch produced in (1) or the ripe compost of Production Example 6 at various ratios shown in Table 2, the water content of each mixture was measured, and tap water was added so that the water content would be 62%, followed by stirring. 1% by weight of the inoculum of Gliocladium roseum IFO 31390 produced in Production Example 2 was inoculated and mixed into each of the obtained cultivation soils, and the mixture was left indoors for 3 days.
After that, the corticium produced in Production Example 3 was added to each medium.
0.5% by weight of the infectious agent of Rolffii was inoculated into each of the soils, and 30 paper pots (made of sugar beet made by Nippon Sugar Beet Co., Ltd.) were packed.

Into this paper pot, seeds of three scallions (cultivar: pale yellow type Kujo green onion, made by Takii seedling) were sown and cultivated in a greenhouse for 1 month. During that time, strains affected by Corticium lolfsi were measured, and the diseased strain rate was calculated by the following formula.

[0043]

[Equation 1] Disease-causing strain rate (%) = {Number of disease-causing strains / (Number of disease-causing strains + Number of healthy strains)} × 100

For comparison, the nursery soil produced in Production Example 4 was mixed with the mature mulch soil produced in Production Example 5 or the mature compost produced in Production Example 6 so that the organic matter content in the mixture was out of the range of the present invention. Cultivation soil was prepared in the same manner as in Examples 5 to 9 except that the above was performed, and a leeks cultivation test was performed in the same manner as in Examples 5 to 9 above.

The test results of the above examples are shown in Table 2 together with the test results of the comparative examples.

[0046]

[Table 2]

As is clear from these results, according to the soil disease control method of the present invention, in which the organic matter is contained in the plant cultivation soil and fungi belonging to the genus Gliocladium are inoculated, the soil for plant cultivation containing almost no organic matter is used. As compared with a comparative example in which a fungus belonging to the genus Gliocladium was inoculated, soil diseases caused by white silkworm fungus on green onions were controlled very efficiently.

[0048]

[Examples 10 to 15] After tap water was added to the ripe mulch produced in Production Example 5 and the ripe compost produced in Production Example 6 until the water content became 48%, 10 kg of each was placed in a pail can and chlorpicrin was added. Covered and kept for 7 days. Then, after 10 days have passed while degassing by removing the lid and occasionally stirring the inside, sterilized water was added thereto to raise the water content to 48%. On the other hand, 10 kg of the matured leaf soil of Production Example 5 and 10% of the matured compost of Production Example 6 each having a water content of 48% in the same manner as above were placed in a pail can and heated by an autoclave until the center of the pail can reached 80 ° C. After that, the mixture was naturally cooled while being kept in the autoclave, and then sterilized water was added thereto to raise the water content to 48%. Further, 10 kg of the matured leaf soil of Production Example 5 and 10% of the matured compost of Production Example 6 each having a water content of 48% in the same manner as described above were placed in pail cans, and thereafter those which were not treated were also prepared.

200 mL of a suspension containing 4.3 × 10 ⁹ cells / mL of the spores of Gliocladium deliquescense IFO9016 obtained in the above Production Example 1 was placed in each of these 6 pail cans. Was added with stirring. Then, the pail can was left still in a constant temperature room at 25 ° C. with the lid lightly covered. The number of fungi belonging to the genus Gliocladium in each pail can content was measured 1 month and 6 months after the start of standing.

For comparison, the nursery soil produced in Production Example 4 was sterilized in an autoclave in the same manner as described above to obtain a water content of 48%.
Then, this was inoculated with Gliocladium deliquescens in the same manner as above, and the same experiment was conducted.

The number of bacteria was measured by the following method. Take 10 g of sample arbitrarily and dilute to 100 mL with sterilized water.
Disperse in Waring blender over 2 minutes. Take 1 mL of this dispersion and dilute it to 0.1% with sterile water.
A fungal selection medium (Smith, VL1990 Phytopothology, 80: 880) belonging to the genus Gliocladium having the composition shown in Table 3
) Was cultured for 1 week at 25 ° C. and the concentration of gliocladium bacteria growing was measured.

[0052]

[Table 3] The results of the examples are shown in Table 4 together with the results of the comparative examples.

[0053]

[Table 4]

As is clear from these results, the fungus belonging to the genus Gliocladium applied to the cultivation soil containing organic matter by the method of the present invention was applied to the cultivation soil containing almost no organic matter of Comparative Example. Compared to fungi belonging to the genus Gliocladium, even if the soil was not autoclaved or chlorpicrin-treated, it showed good growth and colonization. Can be said to be.

[0055]

INDUSTRIAL APPLICABILITY When the soil disease control method of the present invention is used in a method for controlling soil diseases of plants by applying a fungus belonging to the genus Gliocladium to soil for plant cultivation,
It is possible to efficiently control the soil diseases of cultivated plants by sustaining them by enhancing the proliferative property, colonization property and storability of fungi belonging to the genus Gliocladium in the soil for cultivating plants.

Claims (4)

[Claims] 1. A method for controlling soil diseases of plants by applying a fungus belonging to the genus Gliocladium to the soil for plant cultivation, wherein the organic soil is added to the soil for plant cultivation in a dry weight of 5% by weight based on the total amount of the soil. A method for controlling soil diseases of plants, which comprises blending as described above. 2. A method for controlling a soil disease of a plant according to claim 1, wherein the organic matter is mainly mixed with the plant-cultivating soil as a plant-derived organic matter-containing material. 3. The method for controlling soil diseases according to claim 2, wherein the material containing a plant-derived organic matter is selected from peat moss, wood chips, pulp dregs, ripe mulch and ripe compost. 4. Soil diseases include solanaceae, cucurbitaceae, azalea,
The method for controlling a pest according to claim 1, which is a disease for vegetables selected from the family Liliaceae.