JPH0873344A - Enzyme-containing wax capsule - Google Patents

Abstract

PURPOSE: To obtain the subject capsule excellent in storage stability, effectively functioning as a scrubbing agent because of being collapsed with a proper physical stress, and useful as a face-washing agent by dispersing an enzyme in paraffin wax, candelilla wax or the like, cooling and filtering. CONSTITUTION: This capsule is prepared by dispersing an enzyme selected from (D) protease, lipase, and muramidase in the thermally melted mixture of (A) paraffin wax, (B) candelilla wax, and (C) carnauba wax, dispersing the dispersion in hot water, cooling the dispersion, and subsequently filtering the cooled dispersion. The components A, B and C are preferably used in amounts of 20-70wt.%, 10-50wt.% and 5-40wt.%, respectively, on the basis of the whole amount of the waxes, and the component D is preferably used in an amount of 0.1-10wt.% based on the final composition. The diameter of the capsule is preferably 0.2-1mm.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to an improved enzyme-encapsulating wax capsule composition for scrubbing agents.

[0002]

2. Description of the Related Art Conventionally, commercial products have been developed by incorporating enzymes such as protease, lipase, cellulase and muramitase into detergents such as household detergents and facial cleansers.

As an enzyme suitable for these purposes, an enzyme that is stable under ordinary storage conditions and retains its activity for a long period of time is desirable. In recent years, some relatively stable enzymes have been found by making full use of technologies such as screening of enzymes produced by various bacteria and gene manipulation, and they have been applied to powder detergents, etc. A stable enzyme has not yet been found in a system in which water coexists.

In order to solve these problems, attempts have been made to chemically modify the enzyme with polyoxyethylene glycol, polysaccharides or the like, or an enzyme which is embedded in sericin, fibroin, lauromacrogol or the like to be a water-insoluble substance. Stabilization technology is known. However, although these techniques can stabilize the enzyme, they have a problem that the applicable formulations are limited by those immobilized on a water-insoluble carrier.

In order to solve these problems, there is a technique of stabilizing by encapsulating with polysaccharides such as carrageenan, methyl cellulose, amylopectin, hydroxypropyl cellulose and cellulose derivatives, water-soluble polymers such as gelatin and polyvinyl alcohol. Are known.
However, if these capsules are blended in an aqueous formulation, not only the capsules swell due to long-term storage, the entrapped enzyme is inactivated, but also the formulation is not preferable.

It is known that the surface of the capsule is insolubilized for the purpose of preventing swelling of the capsule, but it is disintegrated by an appropriate physical stress and the enzyme is released during use, and the encapsulated enzyme is stable for a long period of time. There was no.

On the other hand, a scrubbing agent is blended in the face wash for the purpose of enhancing the dirt removal efficiency during face washing. Conventional scrubbing agents include polyethylene beads, crystalline cellulose, walnut shells, sawdust, and other hard scrubs, and agar,
Soft scrubs such as polyacrylates and alginates have been used.

However, conventional hard scrubs are often accompanied by irritation or foreign body sensation during use, and soft scrubs are often swelled in the preparation and adversely affect the system, or are not effective during use. A scrubbing agent having an appropriate hardness and stable in the formulation has been demanded.

That is, the object of the present invention is to provide a scrub having a proper scrubbing effect at the time of use because the encapsulated enzyme is stable for a long period of time even if it is blended with an aqueous facial cleanser, and the capsule does not swell excessively in the preparation. An object is to provide an enzyme-containing wax capsule composition for a drug.

[0010]

[Means for Solving the Problems] In order to achieve the above object, the first aspect of the present invention is to provide a method in which paraffin wax, candelilla wax and carnauba wax are heated and mixed and dissolved, and an enzyme selected from protease, lipase and muramitase. Is a wax capsule containing an enzyme for a scrub agent, which is obtained by dispersing and mixing, and then adding hot water to disperse and mix, and cooling and filtering.

According to a second aspect of the present invention, based on the total amount of wax, the paraffin wax content is 20 to 70.
% By weight, candelilla wax content 10-50
The enzyme-containing wax capsule according to claim 1, wherein the blending ratio of the carnauba wax and the carnauba wax is 5 to 40% by weight.

Furthermore, claim 3 of the present invention is the enzyme-encapsulating wax capsule according to claim 1, wherein the blending ratio of the enzyme is 0.1 to 10% by weight based on the final composition.

The structure of the present invention will be described in detail below. The enzyme-encapsulated wax capsule of the present invention is obtained by mixing and dissolving paraffin wax, candelilla wax and carnauba wax in a specific ratio, adding the enzyme and warm water to disperse, cooling, filtering, washing with water and drying. can get.

Paraffin wax used in the present invention,
Candelilla wax and carnauba wax are usually used in cosmetics, and their origin and manufacturing method are not particularly limited.

Paraffin wax, candelilla wax and carnauba wax are mixed in a specific mixing ratio, and the paraffin wax is 20 to 70% by weight and the candelilla wax is 10 to 50% by weight, based on the total amount of the wax.
It is preferable to use carnauba wax in a blending ratio of 5 to 40% by weight. Outside this range, the capsules may become too hard or too soft, which may affect the expression of enzyme activity, or may cause coloring or odor due to wax.

Examples of the enzyme used in the present invention include protease, lipase and muramitase. Also,
An immobilized enzyme in which these enzymes are immobilized on a carrier such as silk fibroin, polyethylene glycol (hereinafter abbreviated as PEG) or dextran can also be used.

The amount of the enzyme incorporated in the composition is preferably 0.1 to 10% by weight based on the total amount of the final composition. If it is less than 0.1% by weight, expression of enzyme activity cannot be expected in a preparation containing the present capsule composition. If the amount exceeds 10% by weight, capsule molding becomes difficult.

The particle size of the capsule is within a range in which the amount of the enzyme to be encapsulated is not limited, the productivity is good, the feeling of foreign matter is not felt during use, and the organoleptic properties of the scrubbing agent are excellent. 3 mm is preferable, and 0.2 is more preferable.
~ 1 mm.

The enzyme-encapsulating wax capsule of the present invention can be prepared, for example, by the following method. That is,
After the wax mixture with a specific blending ratio is heated and dissolved at 60 to 65 ° C, the enzyme with a specific blending ratio is added, and 0.5 to 10 times the amount of warm water at 65 ° C is added to the weight of the wax to mix them uniformly. Spread. The wax is solidified by cooling in a dispersed state. After filtration, washing with water and drying, the target enzyme-containing wax capsule can be obtained. If the amount of warm water is less than 0.5 parts by weight, dispersion becomes difficult, and 10
Even if the amount is more than 100 parts by weight, the effect commensurate with the composition cannot be expected.

The enzyme-encapsulated wax capsule of the present invention is applied to face wash such as body soap, cleansing cream, massage cream, cleansing lotion, toilet soap and creamy soap.

[0021]

The present invention will be described in detail below with reference to Examples and Comparative Examples. In the examples,% means% by weight unless otherwise specified.

The storage stability test and sensory evaluation as a scrubbing agent used in the present invention are as follows.

(1) Storage stability test 1 g of a sample was dissolved or dispersed in 100 ml of water and then stored at 40 ° C.
The enzyme activity immediately after and 1 month after the storage was stored in the thermostatic layer of and was shown as a percentage of the enzyme activity after 1 month with respect to the enzyme activity immediately after.

(2) Sensory evaluation Practical tests were conducted by 20 male and female panelists aged 20 to 40. That is, the face was massaged using a 3% aqueous dispersion of the sample, and in terms of hardness, "soft", "moderate",
"Hard", and "effective" in the scrub effect item,
The number of respondents who answered “no effect” and “feeling of foreign matter” was evaluated.

Examples 1 to 4 and Comparative Examples 1 to 5 Enzyme-containing wax capsules were prepared according to the composition of the present invention shown in Table 1 and the composition for comparison shown in Table 2, and the above storage stability test and sensory test were conducted. An evaluation was carried out.

[0026]

[Table 1]

[0027]

[Table 2]

Manufacturing Method Waxes having the composition ratios shown in Tables 1 and 2 above were heated and dissolved at 60 to 65 ° C. and uniformly mixed, and then the enzyme blending ratios in Tables 1 and 2 were based on the total amount of the final composition. The enzyme was added to the mixture so that the amount of the hot water at 65 ° C. was twice the amount of the wax, and the mixture was uniformly mixed and dispersed. The wax was solidified by cooling in a dispersed state. After filtration, it was washed with water and dried to obtain the desired enzyme-encapsulating wax capsule.

Table 3 shows the results of the storage stability test and sensory evaluation of the example and Table 4 of the comparative example, respectively.

[0030]

[Table 3]

[0031]

[Table 4]

As shown in Table 3, the enzyme-encapsulated wax capsule of the present invention was excellent in the storage stability of the encapsulated enzyme and the scrubbing effect.

Particle size 4 m using only paraffin wax
In Comparative Example 1 in which m was set, the enzyme activity was maintained, but the capsules were soft and there were many panelists who did not have a scrubbing effect or felt a foreign substance.

In Comparative Example 2 using only candelilla wax, many panelists felt a foreign substance because it was too hard. In addition, the capsules were discolored and had an odor, which was not preferable. Further, the enzyme activity could not be confirmed because the amount of the enzyme blended was as low as 0.01%.

In Comparative Example 3 containing only carnauba wax and 12% of enzyme, the capsule was easily disintegrated, so that it felt soft and had no scrubbing effect. In addition, the stability of the enzyme was poor.

Comparative Example 4 using paraffin wax and candelilla wax was excellent as a scrubbing agent, but was inferior in enzyme stability. Also, discoloration and odor that are thought to be due to candelilla wax were observed,
It was not sensually preferable.

Comparative Example 5 using candelilla wax and carnauba wax and having a particle size of 0.05 mm did not have a scrub effect because it was too fine.

[0038]

EFFECTS OF THE INVENTION As described above, the enzyme-encapsulated wax capsule composition of the present invention has excellent storage stability and effectively functions as a scrubbing agent because it disintegrates under moderate physical stress.
It is clear that it is effective when applied to facial cleansers.

Claims (3)

[Claims] A paraffin wax, candelilla wax, and carnauba wax are mixed by heating and dissolved, and then an enzyme selected from protease, lipase, and muramitase is dispersed and mixed, and then warm water is added and dispersed and mixed, followed by cooling and filtering. Enzyme-containing wax capsule for scrubbing agent. 2. A paraffin wax compounding ratio of 20 to 70% by weight, a candelilla wax compounding ratio of 10 to 50% by weight, and a carnauba wax compounding ratio of 5 to 40% by weight based on the total amount of wax. The enzyme-containing wax capsule according to 1. 3. The enzyme-containing wax capsule according to claim 1, wherein the blending ratio of the enzyme is 0.1 to 10% by weight based on the final composition.