JPH0987682A - Contact lens cleaning agent and cleaning / disinfecting method - Google Patents

Abstract

(57) Abstract: An enzyme-based contact lens cleaning agent for cleaning protein stains and lipid stains at the same time, which maintains stable activity for a long time, and one-step contact lens cleaning of the contact lens detergent. Providing a contact lens cleaning and disinfecting method for use with a disinfectant container. SOLUTION: The contact lens detergent is an enzyme aqueous solution in which the mixing ratio of enzyme protease and lipase is 2: 1 to 1: 2, and placenta extract is added in an amount of 0.1 to 0.
5g / 100ml of total amount is mixed, and the pH of the solution is 4.5-
Adjust to 6.0. Further, the cleaning and disinfecting method combines the contact lens cleaning agent and a one-step contact lens cleaning and disinfecting container to efficiently perform cleaning and disinfection in a short time.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a long-term stable contact lens cleaning agent and a cleaning / disinfecting method combining the contact lens cleaning agent and a contact lens cleaning / disinfecting device.

[0002]

2. Description of the Related Art Contact lenses are not transparent when they are worn or handled, because stains such as proteins, lipids and inorganic substances existing in tears and fingertips during handling adhere to and deposit on the surface of the contact lens. If the dirt on the lens surface becomes severe, the cornea becomes inflamed. Therefore, when mounting the contact lens, it is necessary to regularly clean and clean the dirt attached to the surface of the contact lens.

Among these stains, the use of an aqueous solution of enzyme or hypobromite is known to remove protein stains.
It is known to use an aqueous solution of a single surfactant or a combination of different surfactants to remove lipid stains, and to use an aqueous solution of EDTA ・ 2Na etc. to remove inorganic stains and prevent adhesion. Has been.

Further, as a method for cleaning and disinfecting a contact lens with a cleaning agent for an enzyme solution, conventional cleaning and sterilizing treatments are performed in two steps, and these steps are performed in one step. The method of doing it in a short time is shifting.

[0005]

In recent years, the use of an enzyme has become popular as a cleaning agent for contact lenses, but the mainstream is one that uses a protease that is an enzyme for cleaning protein stains alone. There is. In addition to this, there is also a combination of amino acids, but since this is also a kind of protein, it can be a substrate for protease.

On the other hand, it is known to use an enzyme lipase for cleaning lipid stains on contact lenses. However, it is difficult to keep lipase in a protease for a long period of time, and storage at low temperature is the only method. Is an effective means of.
However, it is almost impossible to always store at low temperature in general-purpose products. Therefore, in order to wash lipid stains, it is usually necessary to use a surfactant alone or a combination of different surfactants. A method of relying on the detergency or decomposing lipids with an oxidizing agent or the like has been adopted, and at present, a contact lens detergent combining the enzyme protease and lipase has not been put into practical use.

[0007] Further, sugar alcohols (eg sorbitol), glycerin, calcium salts, borate, etc. are known as stabilizers for the enzyme protease. Can not bear the storage of the period.

An object of the present invention is to eliminate the above-mentioned disadvantages of the conventional examples, to cleanse protein stains and lipid stains at the same time, and to maintain stable activity for a long period of time, and an enzyme-based contact lens detergent, and the contact lens. It is an object of the present invention to provide a method for cleaning and disinfecting contact lenses, which is a combination of a cleaning agent and a contact lens cleaning / disinfecting device capable of cleaning and disinfecting in one step.

[0009]

Means for Solving the Problems In order to achieve the above object, the present invention firstly comprises adding a placenta extract as an enzyme stabilizer to an aqueous solution of an enzyme in which a contact lens detergent comprises protease and lipase. The pH was adjusted, secondly, the mixing ratio of protease and lipase was 2: 1 to 1: 2, and thirdly, the placenta extract was 0.1 to 0.5 g / total 10 as an enzyme stabilizer in the enzyme aqueous solution.
0ml, 4th, pH of the solution was adjusted to 4.5-6.0, 5th, contact lens cleaning and disinfecting method, placenta extract was added to enzyme aqueous solution consisting of protease and lipase as enzyme stabilizer. Further, the gist is to perform cleaning and disinfection by combining a contact lens cleaning agent having a pH adjusted of the solution and a contact lens cleaning / disinfecting device capable of cleaning and disinfecting in one step.

According to the first to fourth aspects of the present invention, the contact lens cleaning agent comprises 0.1% placenta extract as an enzyme stabilizer in an aqueous enzyme solution comprising a protease and a lipase in a mixing ratio of 2: 1 to 1: 2. ~ 0.5 parts by weight was added, and the pH of the solution was adjusted to 4.5 ~ 6.0.
Even after storage under conditions of ° C, humidity of 70% and period of 6 months (corresponding to storage for 3 years at room temperature), the residual activity ratio of protease and lipase is high and the storage stability is excellent.

According to the present invention of claim 5, a placenta extract is added as an enzyme stabilizer in an amount of 0.1 to 0.1 in an aqueous enzyme solution containing a protease and a lipase in a mixing ratio of 2: 1 to 1: 2.
By mixing 0.5 parts by weight and a contact lens cleaning agent with a solution pH adjusted to 4.5 to 6.0 and a contact lens cleaning / disinfecting device that can be cleaned and disinfected in a single step, dirty contact lenses can be removed in a short time. It can be efficiently cleaned and disinfected.

[0012]

[Embodiment]

<Sample used for test> 1. As the enzyme protease, Clear Lens-Pro-2.5MG manufactured by Novo Nordisk BioIndustry was used. 2. As the enzyme lipase, clear lens-lipo-200L manufactured by Novo Nordisk BioIndustry was used. 3. As the placenta extract, a water-soluble placenta extract (mixture of amino acid and enzyme extracted from placenta of animal) manufactured by Nichirei was used. 4. Commercially available sorbitol and pH adjuster citrate-phosphate buffer were used, and purified water was ion-exchanged water.

<Method of measuring residual activity rate of enzyme> Protease was measured by the following basic concept called DMC method and lipase was called pH stat method, and the activity value of enzyme at the time of adjustment was set to 100 (%). The activity value over time was shown by the residual activity rate (%).

DMC Method DMC (dimethylcasein) is hydrolyzed by an enzyme protease to a low molecular weight peptide, and the terminal amino group in this peptide quantitatively reacts with trinitrobenzenesulfonic acid to produce a coloring substance. . The concentration of this coloring substance is measured by measuring the absorbance at 425 nm with an absorptiometer, and the back-calculation is performed to obtain the protease activity rate.

PH Stat Method The pH change of the solution caused by the decomposition of lipids by lipase is detected by an electrical or mechanical system, and the pH is set again by automatically adding an alkali or an acid.
After returning to H, the activity ratio of lipase is determined by titration of alkali or acid with respect to the reaction time.

<Method of measuring light transmittance of contact lens> UV-Visible Recording Spectrophot manufactured by Shimadzu Corporation
The light transmission of each contact lens in the visible light range of 500 nm is measured by ometer UV160. In the measurement results, the light transmittance of 94% or more was considered to be clean.

[0017]

EXAMPLES The present invention will be specifically described below with reference to Examples and Comparative Examples, but this is for specifically explaining the effects of the present invention, and the present invention is not limited thereto. . In Examples 1 to 5, the composition ratio of the enzyme protease and lipase was changed to 3: 1 to 1: 3, and the contact lens cleaning agent having the composition shown in Table 1 below was prepared, and the temperature was 40 °.
Table 1 shows the results of measuring the residual activity rate of each enzyme after 1 month of storage at C and a humidity of 75%.

[0018]

[Table 1] From the results in Table 1, it is considered that the mixing ratio of the protease and the lipase is preferably 2: 1 to 1: 2 in which the residual activity ratio of the protease and the lipase is 55% or more, for the preservability.

In Examples 6 to 11, an aqueous enzyme solution having a ratio of enzyme protease to lipase of 1: 1 was adjusted to pH 4. by using a citric acid-phosphate buffer solution as a pH adjuster.
The contact lens cleaning agent having the composition shown in Table 2 below was prepared by changing it from 0 to 6.5, and the residual activity ratio of the enzyme after 6 months of storage at 40 ° C and 75% humidity was measured. Shown in 2.

[0020]

[Table 2] From the results shown in Table 2, it can be seen that the residual activity ratio of the protease and lipase of Examples 7 to 10 in which the pH of the solution was adjusted to 4.5 to 6.0 was 68% or more, which was effective for storage stability.

In Examples 12 to 16, placenta extract was added to the formulation of Example 3 as an enzyme stabilizer in an amount of 0 to 0.5 g / total amount of 10
Adjust the contact lens cleaning agent according to the formulation in Table 3 below by adding it so that the amount becomes 0 ml, and adjust the temperature to 40 ° C.
Table 3 shows the results of measuring the residual activity rate of the enzyme after 6 months of storage at C and a humidity of 75%.

[0022]

[Table 3] The results shown in Table 3 show that in Examples 13 to 16 in which 0.1 to 0.5 g of placenta extract was added as an enzyme stabilizer, the residual activity ratio of protease and lipase was 83% or more, and excellent preservability was maintained. are doing.

In Examples 17 to 21, the formulation of Example 7 was supplemented with 0 to 0.5 g of placenta extract as an enzyme stabilizer / total amount of 10
The contact lens cleaning agent having the composition shown in Table 4 below was prepared at a temperature of 40 ° C.
The results of measuring the residual activity of the enzyme after 6 months of storage at a humidity of 75% are shown in Table 4.

[0024]

[Table 4] Looking at the results in Table 4, 0.1 to 0.5 g / 100 ml of placenta extract was added as an enzyme stabilizer and the pH of the solution was adjusted to 4.5.
In each of Examples 18 to 21 prepared as above, the residual activity ratio of protease and lipase was 96% or more, and excellent preservability is maintained.

In Examples 22 to 26, 0.2 g of placenta extract as an enzyme stabilizer / 100 m in total amount was added to an aqueous enzyme solution in which the mixing ratio of the enzyme protease and lipase was 2: 1 to 1: 2.
l After adding and adjusting the pH of the solution to 4.5, store the contact lens cleaning agent at a temperature of 40 ° C and a humidity of 75% for 6 months, then use Aseptron, a commercially available one-step cleaning and disinfecting unit.
(Bausch & Lomb) and Micro-Bio-Lizer (Seiko Contact Lens Co., Ltd.) were used to perform a cleaning and disinfection test with a dirty soft contact lens worn for 1 day, and the light transmittance of the soft contact lens before and after cleaning was measured. The results are shown in Table 5.

In the cleaning and disinfecting method here, in Aseptron, the temperature of the cleaning liquid is heated from room temperature to 80 ° C., and kept at this temperature for about 10 minutes, and then cooling is started about 60 minutes later.
The cycle is complete. On the other hand, Micro-Bio-Lize
In r, the temperature of the cleaning liquid is heated from room temperature to 60 ° C, held at this temperature for about 60 minutes, further heated to 80 ° C, held at this temperature for about 20 minutes, and then cooling is started. One cycle is completed after about 100 minutes.

[0027]

[Table 5] Looking at the results in Table 5, in all the test results of Examples 22 to 26, the light transmittance after washing of the soft contact lens washed with the contact lens detergent of the present invention was
It shows 94.5% or more, and cleaning and disinfection can be effectively performed using a commercially available one-step heat disinfection unit.

[0028]

As described above, the contact lens cleaning agent of the present invention can be treated with the enzyme even after being stored under the conditions of a temperature of 40 ° C., a humidity of 70% and a period of 6 months (equivalent to storage at room temperature for 3 years). The residual activity ratio of protease and lipase is 96% or more, and it has excellent preservability.

Further, by combining the contact lens cleaning agent of the present invention with a contact lens cleaning / disinfecting container capable of cleaning and disinfecting in one step, the light transmittance after cleaning is 94.5 even without cleaning by hand scraping or the like. %
As described above, cleaning and disinfection can be efficiently performed in a short time.

Claims (5)

[Claims] 1. A placenta extract is added as an enzyme stabilizer to an aqueous enzyme solution consisting of protease and lipase,
Furthermore, the contact lens cleaning agent characterized in that the pH of the solution is adjusted. 2. The mixing ratio of protease and lipase is 2:
The contact lens cleaning agent according to claim 1, which has a ratio of 1 to 1: 2. 3. The contact lens cleaning agent according to claim 1, wherein the amount of the enzyme stabilizer, placenta extract, is 0.1 to 0.5 g / 100 ml in total. 4. The contact lens cleaning agent according to claim 1, wherein the pH of the solution is adjusted to 4.5 to 6.0. 5. A placenta extract is added as an enzyme stabilizer to an enzyme aqueous solution consisting of protease and lipase,
Furthermore, the cleaning and disinfecting method is characterized in that cleaning and disinfection are performed by combining a contact lens cleaning agent having a pH adjusted of a solution and a contact lens cleaning and disinfecting device capable of cleaning and disinfecting in one step.