JPH10127164A - Cultivation method of white truffle - Google Patents

Abstract

PROBLEM TO BE SOLVED: To cultivate white truffles and to made mass production of the white truffles having high quality without being affected by the weather and climate by growing the hyphae of white western truffles in a medium mixing with the hot water extraction liquid of carrots and obtaining the basidio carp thereof. SOLUTION: The hyphae of the ascomycetes Tuberaceae, scientific name white western truffle (Rhizopogon rubescens (TUL.)TUL). is grown in the medium 1 mixed with the hot water extraction liquid of the carrots and the fruit bodies 5 thereof are obtd. The white truffles are then cultivated. The hyphae described above are preferably grown at the prescribed medium 1 and are further cold dried at 5 to 10 deg.C.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a method for cultivating white truffles.

[0002]

2. Description of the Related Art Ascomycetes, Aspergillus oryzae, Scientific name White western ginger (Tuber.magnatum: commonly called white truffle) are prized in foreign countries, particularly in France and Italy.

[0003] White truffles are mycorrhizal fungi parasitic on beech roots, and the fruiting bodies become massive. So far, there have been reported methods of sowing spores in soil and cultivating them, inoculating white truffle hypha into cotton, attaching the cotton to beech trees, and burying them in the soil as they are. There are no successful cases yet, and all of these methods use natural materials, are not completely artificial cultivation methods, are influenced by weather and climate, and do not fit into mass production.

[0004] As a result of intensive studies, the present inventor has completed a method of artificially cultivating white truffles, which has been considered impossible so far.

[0005]

The gist of the present invention will be described with reference to the accompanying drawings.

[0006] Ascomycetes / Agaricaceae
Medium 1 in which mycelia of white western ginger wax (Tuber.magnatum: commonly known as white truffle) are mixed with hot water extract of carrot.
The present invention relates to a method for cultivating white truffles, wherein the fruiting body 5 is obtained by growing the same.

[0007] In addition, after growing hyphae of Ascomycetes, Asteraceae, and White name of white western ginger (Tuber.magnatum: commonly known as white truffle) in a predetermined medium 1, they are further cooled and dried at 5 to 10 ° C. The present invention relates to a method for cultivating white truffles, which is characterized by the following.

[0008] In addition, the medium 1 containing a hot water extract of carrots contains Ascomycetes, Agaricaceae, Scientific name white western ginger (Tuber.magnatum: commonly called white truffle)
The seedlings are then inoculated and grown, and then surrounded by air-permeable soil 4 and allowed to stand at 5 to 25 ° C. for about two months to grow fruiting bodies 5. And a method for cultivating white truffles.

[0009] In addition, medium 1 containing a hot water extract of hardwood chips such as beech, corn, etc., rice bran, yeast, okara, glucose, and carrots is mixed with Ascomycetes, Asteraceae, Scientific name White Western Shower ( Tuber. Magnatum (commonly known as white truffle) is inoculated and propagated, and then surrounded by soil 4 having a particle diameter of about 2 to 10 mm and left in this state at 5 to 25 ° C. for about 2 months to obtain fruiting bodies. 5 and then growing the fruiting body 5 at 5-10 ° C for at least 1
The present invention relates to a method for cultivating white truffles, which is allowed to stand for months.

[0010] In addition, ascomycetes, Agaricaceae and Scientific name white western ginger (Tuber.magn) in a medium containing carbohydrates such as glucose and mannose as a carbon source.
atum (commonly called white truffle) is inoculated and proliferated, and then set as dark as possible and allowed to stand for about two months to grow the fruiting body to obtain the fruiting body. It relates to a method for cultivating truffles.

[0011] In addition, aspergillus fungi, Asteraceae, Scientific name white western ginger (Tuber.magn) in a medium containing carbohydrates such as glucose and mannose as a carbon source.
atum (commonly known as white truffle) is inoculated and proliferated, and then allowed to stand at an ambient temperature of 5 to 25 ° C. for about two months to grow fruiting bodies and obtain the fruiting bodies. Pertaining to a cultivation method.

[0012] In addition, hyphae of Ascomycetes, Acacia oleraceae, and white trout (scientific name: white truffle) are grown on a medium containing carbohydrates such as glucose and mannose as a carbon source. The present invention relates to a method for cultivating white truffles, wherein a fruiting body is grown and the fruiting body is obtained.

The present invention is an invention obtained by repeating various experiments, and the claims summarize the results of the experiments.

[0013] Therefore, the present invention can completely cultivate white truffles, and can mass-produce the rare white truffles.

[0014]

BEST MODE FOR CARRYING OUT THE INVENTION

 <Experimental example 1> The medium composition is as follows.

Hot water extract of carrot and a mixture of 600 g of appropriately mixed hardwood chips such as beech and corn, 30 g of rice bran, 5 g of dry yeast (trade name: Ebios), 30 g of okara, 1 g of urea, and 20 g of glucose Carrot 2
00 g with 1 liter of water and heated at a heating temperature of about 100 ° C. for 20 to
Hot water extraction for 30 minutes (Add 1 liter when water is low.
)) To prepare a culture medium 1 adjusted to have a water content of about 60% by weight.

The culture medium 1 contains sodium hydroxide (lime),
The pH is adjusted to 6.5 to 7.5 by mixing urea. This is the optimal pH value found as a result of the experiment.

This medium 1 (about 550 to 600 g) is placed in a container 2 having a capacity of 850 cc, and after sterilization,
Inoculated with the mycelium of ascomycetes, Gingerbills, Scientific name white western ginger wax (Tuber.magnatum: commonly known as white truffle).

This mycelium is a mycelium isolated by agriculture and forestry fungi (Farming and Forest Fungi F-5-303) by air-floating a fresh white truffle fruit body collected in Italy.

The method of obtaining mycelia from the fruit bodies of raw white truffles is based on a known tissue separation method. Specifically, an inner meat piece free from various germs is taken from a raw truffle fruit body, and the meat piece is placed in a sterilized test tube. This test tube is filled with a medium of the above composition solidified with agar. When left in this state at 20 ° C. to 25 ° C. for about 20 days, mycelium (original species) of white truffle is formed. This original species is distributed to a plurality of containers, and the mycelium is grown by the same treatment.

After 15 to 30 days, the inside of the container 2 becomes white, and the culture (proliferation) of the mycelium is completed.

Next, the container 2 after completion of the culture of the mycelium is placed in a container 3 having a capacity of 30 cm × 30 cm × 30 cm.
The surroundings are surrounded by soil 4 (water content is adjusted to about 50% by weight).

This soil 4 is preferably unfertilized red soil. This is because bacteria grow on fertile soil. The soil 4 is desirably rough soil having a particle diameter of about 2 to 10 mm in order to secure air permeability. Furthermore, this soil 4 makes the distance a as long as possible as shown in FIG.
cm or more), so that the soil 4 is not dried so quickly.

When left in this state at 20 to 25 ° C. for about two months, a massive fruit body 5 (white truffle) is obtained from the medium 1.
About 2 to 3 small lumps of about 5 g and about 1 to 2 large lumps of about 30 to 40 g were generated (see FIG. 3).

[0024] The inventor collected this mass and cracked it. As a result, it was confirmed that no spores had yet been formed inside the mass, and that at this stage there was much water and it was too hard.

Therefore, the inventor has determined that it is necessary to further age this lump, and has performed the following operation.

The mass was left in a refrigerator at 5 to 10 ° C. for 1 to 4 months to cool and dry.

Thereafter, when this lump was taken out and cracked, it was found that the moisture had ceased, and that sufficient white spores were formed inside the lump to give it a moderate softness.

When this truffle was boiled and eaten, it was a white truffle commercially available.

After the cultivation in the container 2 is completed, the medium 1 is scraped out together with the mycelium in the container 2 into the container 3, and the soil 4 is similarly put into the container for cultivation to obtain white truffle. Make sure that.

<Experimental Example 2> A mycelium isolated from the fruit body of white truffle collected from Oshirakawa Nitta, Irihirose-mura, Kitauonuma-gun, Niigata Prefecture, was cultured and grown according to Experimental Example 1. The same truffle as in Example 1 was obtained.

<Experimental Example 3> D. Y. A
Medium, pH 7. D. Y. A medium containing 0.09% of dipotassium hydrogen phosphate, ammonium dihydrogen phosphate and magnesium sulfate, respectively. D. Y. M. A plate medium, pH 3.0 with 1N-NaCl and 1N-HCl
P. adjusted to 8.0. D. Y liquid medium;
D. D-glucose, which is a carbon source in the L medium, was replaced with D-xylose, D-mannose, D-galactose, D-maltose, and D-sucrose (all of which were made by Wako Pure Chemical Industries). When the cells were cultured and grown according to Experimental Example 1, the same truffles as in Experimental Example 1 were obtained.

Further, P.S. D. As a carbon source of the L medium,
Those using D-mannose had the best growth of mycelia.

Incidentally, P.S. D. The pH of the Y liquid medium is 5.0
The one adjusted to ~ 7.0 showed particularly good growth of hypha.

Further, when a culture medium 1 containing other carbohydrates such as rice bran and bran was used and cultured and grown according to Experimental Example 1, truffles were obtained as in Experimental Example 1.

Therefore, it is presumed that any medium used for culturing white truffles may be used as long as it contains carbohydrates as a carbon source.

<Experimental Example 4> Instead of the container 2, the medium 1 was placed in a polypropylene bag (packed with 2.5 kg, inoculated with 40 g).
, And cultured and grown in a room having a humidity of 75% in accordance with Experimental Example 1. Truffles were obtained in the same manner as in Experimental Example 1.

<Experimental Example 5> 2 cultured according to Experimental Example 4
Breaking the polypropylene bag of the individual bacterial bed, planting the internal bacterial bed in outdoor soil and leaving it for about two months, 16-33m
14 fruit bodies of m (total weight: 600 g) were confirmed.
The inventor has also confirmed that the growth of the fruiting body is not related to the season.

When the soil was grown using field soil or mountain soil as outdoor soil, the fruiting body grew to a harvestable size (1 cm or more) in about two months in the same manner as described above.

When the bacterial bed was left as it was without breaking the polypropylene bag, it was confirmed that the fruiting bodies grew along the inner peripheral wall of the polypropylene bag. Therefore, in order to grow white truffles that have good looks and good commercial properties, the bags must be broken.

<Experimental Example 6> When the bacterial bed cultured according to Experimental Example 1 was placed in a room and grown in a state where light was blocked, the growth of fruiting bodies was confirmed in about one month, and the fruiting body was confirmed in about two months. The entity has grown to a harvestable size. Also, the darker the room, the bigger the fruiting body could be harvested.

It is presumed that white truffles usually grow in the soil, and thus grow better in darker places.

The indoor temperature was set at a substantially constant temperature in the range of 5 to 25 ° C., and the growth condition was repeatedly confirmed. It was confirmed that it grew. When the temperature was set at 17 ° C., the fruiting body grew the largest. It was also confirmed that the fruiting bodies grew to a harvestable size even when the temperature was not constantly adjusted.

Therefore, it was confirmed that there was no need to pay particular attention to the temperature setting when growing white truffles.

[Brief description of the drawings]

FIG. 1 is a process explanatory view of this embodiment.

FIG. 2 is a process explanatory view of the present embodiment.

FIG. 3 is a process explanatory view of the present embodiment.

[Explanation of symbols]

 1 medium 2 volume 4 soil 5 fruiting body

Claims (7)

[Claims] 1. A fungus of Ascomycetes, Asteraceae, Scientific name White western ginger (Tuber.magnatum: commonly known as white truffle) is grown in a medium mixed with a hot water extract of carrot to obtain its fruiting body. A method for cultivating white truffles, characterized in that: 2. After growing hyphae of Ascomycetes, Asteraceae, Scientific name white western ginger (Tuber.magnatum: commonly known as white truffle) in a predetermined medium, a further 5 to 5 minutes.
A method for cultivating white truffles, which is cooled and dried at 10 ° C. 3. A medium mixed with a hot water extract of carrot, inoculated with an ascomycetes, a ginger of the family Asteraceae, and a seed of white western ginger (Tuber.magnatum: commonly known as white truffle), and then grown. Cultivating white truffles, wherein the surroundings are surrounded by air-permeable soil, the fruit body is allowed to grow at 5 to 25 ° C. for about two months, and then the fruit body is cooled and dried. Method. 4. A medium mixed with a hot water extract of hardwood chips such as beech and corn, rice bran, yeast, okara, glucose, and carrot, ascomycetes, Asteraceae, Scientific name White western ginger (Tuber) .magnatum: commonly known as white truffle), inoculated and grown, and then surrounded with soil having a particle diameter of about 2 to 10 mm.
A method for cultivating white truffles, comprising allowing the fruiting body to grow by leaving it at 25 ° C. for about two months, and then leaving the fruiting body to stand at 5 to 10 ° C. for at least one month. 5. A medium containing a carbohydrate such as glucose or mannose as a carbon source, ascomycetes, Asteraceae, Scientific name White western ginger wax (Tuber.magnatu)
m: so-called white truffle), inoculated and grown, and then allowed to stand as dark as possible for about two months to grow the fruiting body to obtain the fruiting body. How to grow truffles. 6. A medium containing a carbohydrate such as glucose or mannose as a carbon source, ascomycetes, Asteraceae, Scientific name White western ginger (Tuber.magnatu)
m: so-called white truffle), which is grown by inoculating a seed bacterium, and then allowed to stand at an ambient temperature of 5 to 25 ° C. for about 2 months to grow fruiting bodies and obtain the fruiting bodies. Cultivation method. 7. A mycelium of Ascomycetes, Acacia cholera, White name of white western ginger (Tuber.magnatum: commonly known as white truffle) is grown on a medium containing carbohydrates such as glucose and mannose as a carbon source. A method for cultivating white truffles, comprising growing fruit bodies and obtaining the fruit bodies.