JPH1017600A5 - - Google Patents

Info

Publication number
JPH1017600A5
JPH1017600A5 JP1996188369A JP18836996A JPH1017600A5 JP H1017600 A5 JPH1017600 A5 JP H1017600A5 JP 1996188369 A JP1996188369 A JP 1996188369A JP 18836996 A JP18836996 A JP 18836996A JP H1017600 A5 JPH1017600 A5 JP H1017600A5
Authority
JP
Japan
Prior art keywords
nacl
eluted
column
equilibrated
edta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1996188369A
Other languages
English (en)
Other versions
JPH1017600A (ja
Filing date
Publication date
Priority claimed from JP8188369A external-priority patent/JPH1017600A/ja
Priority to JP8188369A priority Critical patent/JPH1017600A/ja
Application filed filed Critical
Priority to DE69735039T priority patent/DE69735039D1/de
Priority to PT97110586T priority patent/PT816504E/pt
Priority to EP97110586A priority patent/EP0816504B1/en
Priority to AT97110586T priority patent/ATE315092T1/de
Priority to US08/886,152 priority patent/US5880273A/en
Publication of JPH1017600A publication Critical patent/JPH1017600A/ja
Priority to US09/196,222 priority patent/US6323017B1/en
Priority to US09/961,253 priority patent/US20030040093A1/en
Publication of JPH1017600A5 publication Critical patent/JPH1017600A5/ja
Pending legal-status Critical Current

Links

Description

【００３４】
（２） （１）で得られた上清部分を、ＮａＣｌで１Ｍに調整し、１５分間撹拌した後、予め５０ｍＭ Ｔｒｉｓ−ＨＣｌ（ｐＨ ７．４）、１ｍＭ ＥＤＴＡおよび１Ｍ ＮａＣｌよりなる緩衝液で平衡化したブチルトヨパール６５０Ｍカラムに付した。 カラムを同じ緩衝液で洗浄した後、プロテインをＮａＣｌ（１−０Ｍ）の直線グラジュエントにより溶出した。 ＰＡＦアセチルヒドロラーゼ活性は、１−０Ｍ ＮａＣｌフラクション中において、単一ピークとして溶出された。
【００５０】
【配列表】
【００５１】
【００５２】
【００５３】
JP8188369A 1996-06-28 1996-06-28 血小板活性化因子アセチルヒドロラーゼおよびその遺伝子 Pending JPH1017600A (ja)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP8188369A JPH1017600A (ja) 1996-06-28 1996-06-28 血小板活性化因子アセチルヒドロラーゼおよびその遺伝子
DE69735039T DE69735039D1 (de) 1996-06-28 1997-06-27 Acetylhydrolase des Plättchen aktivierenden Faktors, und entsprechendes Gen
PT97110586T PT816504E (pt) 1996-06-28 1997-06-27 Acetil-hidrolase para o factor activador das plaquetas, e seu gene
EP97110586A EP0816504B1 (en) 1996-06-28 1997-06-27 Platelet activating factor acetylhdrolase, and gene thereof
AT97110586T ATE315092T1 (de) 1996-06-28 1997-06-27 Acetylhydrolase des plättchen aktivierenden faktors, und entsprechendes gen
US08/886,152 US5880273A (en) 1996-06-28 1997-06-30 Platelet activating factor acetylhydrolase, and gene thereof
US09/196,222 US6323017B1 (en) 1996-06-28 1998-11-20 Platelet activating factor acetylhydrolase, and gene thereof
US09/961,253 US20030040093A1 (en) 1996-06-28 2001-09-25 Platelet activating factor acetylhydrolase, and gene thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP8188369A JPH1017600A (ja) 1996-06-28 1996-06-28 血小板活性化因子アセチルヒドロラーゼおよびその遺伝子

Publications (2)

Publication Number Publication Date
JPH1017600A JPH1017600A (ja) 1998-01-20
JPH1017600A5 true JPH1017600A5 (ja) 2004-07-15

Family

ID=16222415

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8188369A Pending JPH1017600A (ja) 1996-06-28 1996-06-28 血小板活性化因子アセチルヒドロラーゼおよびその遺伝子

Country Status (6)

Country Link
US (3) US5880273A (ja)
EP (1) EP0816504B1 (ja)
JP (1) JPH1017600A (ja)
AT (1) ATE315092T1 (ja)
DE (1) DE69735039D1 (ja)
PT (1) PT816504E (ja)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0658205B1 (en) 1993-06-25 2000-03-15 Smithkline Beecham Plc Lipoprotein associated phospholipase a2, inhibitors thereof and use of the same in diagnosis and therapy
SK286518B6 (sk) * 1997-08-13 2008-12-05 Icos Corporation Purifikovaný a izolovaný polypeptidový fragment PAF-AH, spôsob jeho produkcie a použitia, farmaceutická kompozícia, izolovaný polynukleotid, DNA vektor a hostiteľská bunka
WO2000024910A1 (en) * 1998-10-28 2000-05-04 Smithkline Beecham Plc Process of purification of low density lipoprotein associated phospholipase a2 using immobilized metal affinity chromatography
KR19990084203A (ko) * 1999-09-28 1999-12-06 이승렬 이동 전화 단말기의 착신 신호 및 송신 자동 제어 방법 및장치
ATE269342T1 (de) * 2001-12-07 2004-07-15 Sirs Lab Gmbh Verbindungen zur bestimmung der aktivität von phospholipase a2
NZ536228A (en) * 2002-04-15 2008-04-30 Burcon Nutrascience Mb Corp Individual isolated and purified 2S, 7S and 12S canola proteins may be used in conventional applications of protein isolates, such as, protein fortification of processed foods
US6890831B2 (en) * 2002-06-03 2005-05-10 Sanyo Electric Co., Ltd. Method of fabricating semiconductor device
DE602004026439D1 (de) 2003-05-28 2010-05-20 Glaxo Group Ltd Lp-pla2-aktivitätstest mit hohem durchsatz
US7741020B2 (en) 2004-04-16 2010-06-22 Glaxo Group Limited Methods for detecting Lp-PLA2 activity and inhibition of Lp-PLA2 activity
US20150017671A1 (en) 2004-04-16 2015-01-15 Yaping Shou Methods for detecting lp-pla2 activity and inhibition of lp-pla2 activity
US20140283157A1 (en) 2013-03-15 2014-09-18 Diadexus, Inc. Lipoprotein-associated phospholipase a2 antibody compositions and methods of use

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3563091B2 (ja) * 1993-08-03 2004-09-08 第一サントリーファーマ株式会社 酸化リン脂質分解酵素およびその遺伝子
ATE315655T1 (de) * 1993-10-06 2006-02-15 Icos Corp Acethylhydrolase des plättchen aktivierenden faktors
WO1997012984A1 (en) * 1995-09-29 1997-04-10 Smithkline Beecham Plc COMPOUND HAVING SEQUENCE HOMOLOGY WITH LIPOPROTEIN ASSOCIATED PHOSPHOLIPASE A2 (Lp-PLA2)/PAF ACETYL HYDROLASE

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