JPH10182402A - Agent for promoting synthesis of hyaluronic acid - Google Patents
Agent for promoting synthesis of hyaluronic acidInfo
- Publication number
- JPH10182402A JPH10182402A JP8345101A JP34510196A JPH10182402A JP H10182402 A JPH10182402 A JP H10182402A JP 8345101 A JP8345101 A JP 8345101A JP 34510196 A JP34510196 A JP 34510196A JP H10182402 A JPH10182402 A JP H10182402A
- Authority
- JP
- Japan
- Prior art keywords
- hyaluronic acid
- skin
- lavender
- cosmetic
- lavender extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920002674 hyaluronan Polymers 0.000 title claims abstract description 40
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title claims abstract description 39
- 229960003160 hyaluronic acid Drugs 0.000 title claims abstract description 39
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 22
- 238000003786 synthesis reaction Methods 0.000 title claims abstract description 22
- 230000001737 promoting effect Effects 0.000 title abstract description 8
- 239000003795 chemical substances by application Substances 0.000 title abstract description 5
- 229940083980 lavender extract Drugs 0.000 claims abstract description 27
- 235000020723 lavender extract Nutrition 0.000 claims abstract description 27
- 239000002537 cosmetic Substances 0.000 claims abstract description 19
- 239000004480 active ingredient Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 10
- 239000000203 mixture Substances 0.000 abstract description 10
- 244000178870 Lavandula angustifolia Species 0.000 abstract description 7
- 235000010663 Lavandula angustifolia Nutrition 0.000 abstract description 7
- 239000000284 extract Substances 0.000 abstract description 7
- 239000001102 lavandula vera Substances 0.000 abstract description 7
- 235000018219 lavender Nutrition 0.000 abstract description 7
- 230000032683 aging Effects 0.000 abstract description 6
- 239000003814 drug Substances 0.000 abstract description 5
- 229940079593 drug Drugs 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 239000003921 oil Substances 0.000 abstract description 3
- 239000004094 surface-active agent Substances 0.000 abstract description 3
- 239000003906 humectant Substances 0.000 abstract description 2
- 244000025254 Cannabis sativa Species 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 16
- 238000000605 extraction Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 235000019441 ethanol Nutrition 0.000 description 8
- 239000002904 solvent Substances 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 6
- 239000006210 lotion Substances 0.000 description 6
- 239000006071 cream Substances 0.000 description 5
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 4
- 150000001298 alcohols Chemical class 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000037303 wrinkles Effects 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- -1 colorings Substances 0.000 description 3
- 239000012228 culture supernatant Substances 0.000 description 3
- 210000004207 dermis Anatomy 0.000 description 3
- 150000002016 disaccharides Chemical class 0.000 description 3
- 239000002552 dosage form Substances 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 239000003205 fragrance Substances 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000003755 preservative agent Substances 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- 210000001626 skin fibroblast Anatomy 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 229920002683 Glycosaminoglycan Polymers 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000001815 facial effect Effects 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 229940075507 glyceryl monostearate Drugs 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 210000004927 skin cell Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- ALSTYHKOOCGGFT-KTKRTIGZSA-N (9Z)-octadecen-1-ol Chemical compound CCCCCCCC\C=C/CCCCCCCCO ALSTYHKOOCGGFT-KTKRTIGZSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 241000561734 Celosia cristata Species 0.000 description 1
- 102000011413 Chondroitinases and Chondroitin Lyases Human genes 0.000 description 1
- 108010023736 Chondroitinases and Chondroitin Lyases Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 239000005639 Lauric acid Substances 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 210000001520 comb Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002431 foraging effect Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 229940097043 glucuronic acid Drugs 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- KIUKXJAPPMFGSW-MNSSHETKSA-N hyaluronan Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)C1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H](C(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-MNSSHETKSA-N 0.000 description 1
- 229940099552 hyaluronan Drugs 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229940119170 jojoba wax Drugs 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 229940055577 oleyl alcohol Drugs 0.000 description 1
- XMLQWXUVTXCDDL-UHFFFAOYSA-N oleyl alcohol Natural products CCCCCCC=CCCCCCCCCCCO XMLQWXUVTXCDDL-UHFFFAOYSA-N 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000004215 skin function Effects 0.000 description 1
- 230000008491 skin homeostasis Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 210000001179 synovial fluid Anatomy 0.000 description 1
- BORJONZPSTVSFP-UHFFFAOYSA-N tetradecyl 2-hydroxypropanoate Chemical compound CCCCCCCCCCCCCCOC(=O)C(C)O BORJONZPSTVSFP-UHFFFAOYSA-N 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000004127 vitreous body Anatomy 0.000 description 1
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、安全性の高いラベ
ンダー抽出液を有効成分として含有するヒアルロン酸合
成促進剤、好ましくは化粧料の形態に調製したヒアルロ
ン酸合成促進剤に関する。TECHNICAL FIELD The present invention relates to a hyaluronic acid synthesis promoter containing a highly safe lavender extract as an active ingredient, and preferably to a hyaluronic acid synthesis promoter prepared in the form of a cosmetic.
【０００２】[0002]
【従来の技術】高齢化社会を迎えつつある我が国におい
て、近年、加齢とともに増加するしわ、しみなどの皮膚
の老化に対する研究が盛んとなっている。皮膚は、主
に、表皮、真皮、皮下組織に分けられる。特に真皮の主
な構成細胞である線維芽細胞は、コラーゲンなどのタン
パク質やヒアルロン酸などのグリコサミノグリカンを産
生し、結合組織(細胞外マトリックス)を形成し、皮膚の
恒常性維持に重要な役割を果たしている。2. Description of the Related Art In Japan, which is undergoing an aging society, studies on skin aging such as wrinkles and spots, which increase with aging, have been actively conducted in recent years. Skin is mainly divided into epidermis, dermis and subcutaneous tissue. In particular, fibroblasts, the main constituent cells of the dermis, produce proteins such as collagen and glycosaminoglycans such as hyaluronic acid, form connective tissue (extracellular matrix), and are important for maintaining skin homeostasis. Plays a role.
【０００３】ヒアルロン酸は皮膚真皮組織に存在するグ
リコサミノグリカンの中核をなす物質であり、グルクロ
ン酸とＮ−アセチルグルコサミンが交互にβ−グリコシ
ド結合した二糖の繰返し構造を有する分子量数百万に達
する高分子化合物である。このヒアルロン酸は、皮膚、
じん帯、関節液、目の硝子体など、生体に広く分布して
おり、その機能としては、細胞の支持、細胞間隙におけ
る水の保持、皮膚の潤滑性と柔軟性の保持、ならびに、
外力(機械的障害)および細菌感染の防止などが考えられ
ている。また、最近の研究によれば、ヒアルロン酸は単
に支持機能に留まらず、細胞の増殖や情報伝達にも関与
していることが明らかとなっている[後藤 真：炎症, 15
(2), 105-113 (1995)]。[0003] Hyaluronic acid is a core substance of glycosaminoglycan present in skin dermis tissue, and has a molecular weight of several million having a repeating structure of disaccharide in which glucuronic acid and N-acetylglucosamine are alternately β-glycosidically linked. Is a high molecular compound. This hyaluronic acid is found in the skin,
Ligaments, synovial fluid, vitreous body of the eye, etc. are widely distributed in the living body, its functions include cell support, water retention in the intercellular space, maintenance of skin lubricity and flexibility, and
External force (mechanical damage) and prevention of bacterial infection are considered. In addition, recent studies have shown that hyaluronic acid is not only involved in supporting functions, but also involved in cell proliferation and information transmission [Shin Goto: Inflammation, 15
(2), 105-113 (1995)].
【０００４】老化に伴って、皮膚中のヒアルロン酸含量
が減少することが報告されており[Maria O.Longas：Car
bohydrate Research, 159, 127-136 (1987)]、その結
果、小皺やかさつきなどの老化をもたらすと言われてい
る。従来より、皮膚に対するヒアルロン酸の優れた機能
に着目して、ニワトリの鶏冠やストレプトコッカス(Str
eptococcus)属微生物の発酵由来のヒアルロン酸が種々
の化粧料に配合されている。しかし、これらの化粧料は
ヒアルロン酸を皮膚表面に塗布するだけのものであり、
ヒアルロン酸が高分子であるため皮膚から吸収されな
い。従って、化粧料を洗い落とせば効果が消え、根本か
ら皮膚機能を改善するものにはならず、十分な効果が期
待できなかった。このため、ヒアルロン酸産生能を有す
る細胞を活性化して皮膚におけるヒアルロン酸量を増加
させる物質、即ち、ヒアルロン酸合成促進効果を有する
物質が、皮膚の老化に対して有用と考えられ、シワ改善
剤として注目されている。It has been reported that hyaluronic acid content in the skin decreases with aging [Maria O. Longas: Car
bohydrate Research, 159 , 127-136 (1987)], which is said to result in aging, such as fine wrinkles and bulkiness. Traditionally, focusing on the superior function of hyaluronic acid on the skin, chicken cockscomb and Streptococcus (Str
Hyaluronic acid derived from fermentation of microorganisms belonging to the genus eptococcus) has been incorporated into various cosmetics. However, these cosmetics only apply hyaluronic acid to the skin surface,
Hyaluronic acid is a polymer and is not absorbed by the skin. Therefore, if the cosmetic is washed off, the effect disappears, the skin function is not fundamentally improved, and a sufficient effect cannot be expected. Therefore, a substance that activates cells capable of producing hyaluronic acid and increases the amount of hyaluronic acid in the skin, that is, a substance having a hyaluronic acid synthesis promoting effect is considered to be useful for aging of the skin, and a wrinkle improving agent It is attracting attention.
【０００５】[0005]
【発明が解決しようとする課題】本発明の課題は、皮膚
細胞を活性化することによりヒアルロン酸を多量に産生
させ、これによって皮膚の老化を防止し、肌のシワの発
生を予防し、滑らかでしっとりした瑞々しい肌を与える
ことができる、皮膚に対して安全性の高いヒアルロン酸
合成促進剤を提供することであった。An object of the present invention is to activate skin cells to produce a large amount of hyaluronic acid, thereby preventing aging of the skin, preventing the occurrence of wrinkles on the skin, and smoothing the skin. Another object of the present invention is to provide a hyaluronic acid synthesis promoter which can give moist and fresh skin and is highly safe for skin.
【０００６】[0006]
【課題を解決するための手段】本発明者らは、上記課題
を解決すべく、種々の植物抽出液についてスクリーニン
グを行った。このスクリーニングは、正常ヒト皮膚線維
芽細胞に対するヒアルロン酸合成促進効果を指標にして
行った。その結果、ラベンダー抽出液が目的の効果を有
することを見い出し、本発明を完成するに至った。即
ち、本発明は、ラベンダー抽出液を有効成分として含有
するヒアルロン酸合成促進剤を提供するものである。本
発明の好ましい態様においては、該ヒアルロン酸合成促
進剤を化粧料の形態に調製する。Means for Solving the Problems The present inventors screened various plant extracts to solve the above-mentioned problems. This screening was performed using the effect of promoting hyaluronan synthesis on normal human dermal fibroblasts as an index. As a result, they found that the lavender extract had the desired effect, and completed the present invention. That is, the present invention provides a hyaluronic acid synthesis promoter containing a lavender extract as an active ingredient. In a preferred embodiment of the present invention, the hyaluronic acid synthesis promoter is prepared in the form of a cosmetic.
【０００７】[0007]
【発明の実施の形態】抽出に用いるラベンダーは全草で
あってもよいが、地上部、特に花茎部が好ましい。ま
た、ラベンダーを採取後そのまま用いてもよいし、天日
乾燥などの乾燥処理をしたものを用いてもよい。ラベン
ダーの抽出溶媒としては、水または低級アルコール(メ
チルアルコール、エチルアルコールまたはブタノールな
ど)、アセトンもしくは酢酸エチルなどの有機溶媒の１
種または２種以上を適宜混合して使用することができ
る。好ましい抽出溶媒は、水または低級アルコールの単
独、または水と低級アルコールの混合液である。特に好
ましい抽出溶媒は、低級アルコール単独である。BEST MODE FOR CARRYING OUT THE INVENTION The lavender used for the extraction may be whole plant, but the above-ground part, especially the flower stem part is preferable. The lavender may be used as it is after being collected, or may be used after being subjected to a drying treatment such as solar drying. Lavender extraction solvents include water or organic solvents such as lower alcohols (eg, methyl alcohol, ethyl alcohol or butanol), acetone or ethyl acetate.
Species or a mixture of two or more can be used as appropriate. Preferred extraction solvents are water or lower alcohols alone or a mixture of water and lower alcohols. Particularly preferred extraction solvents are lower alcohols alone.
【０００８】抽出は一般的な方法で行ってよく、通常は
上記ラベンダーを上記溶媒に浸漬し、所望により撹拌お
よび／または加熱することにより行う。ラベンダー／抽
出溶媒(重量／容量)の比は、好ましくは１／５００〜１
／２、さらに好ましくは１／１００〜１／５の範囲であ
る。抽出温度は、通常は５〜１００℃、好ましくは５〜
５０℃の範囲であり、抽出時間は、通常、室温付近の場
合は、１〜１４日間であり、５０℃以上の場合は、１〜
４８時間である。[0008] The extraction may be performed by a general method, and is usually performed by immersing the lavender in the solvent and, if desired, stirring and / or heating. The ratio of lavender / extraction solvent (weight / volume) is preferably 1/500 to 1
/ 2, more preferably 1/100 to 1/5. The extraction temperature is usually 5 to 100 ° C, preferably 5 to 100 ° C.
The extraction time is usually 1 to 14 days when the temperature is around room temperature, and 1 to 14 days when the temperature is 50 ° C. or more.
48 hours.
【０００９】このようにして得た抽出液をそのままで本
発明のヒアルロン酸合成促進剤に用いることができる
が、この抽出液を濃縮あるいは適当な溶媒(例えば、水
または低級アルコール)で希釈して用いることもでき
る。好ましくは、上記抽出液を濃縮し、濃縮物に水を加
え、析出した不溶物を除去し、得られた濾液を濃縮ある
いは適当な溶媒で希釈して本発明のヒアルロン酸合成促
進剤に用いる。The extract thus obtained can be used as it is for the hyaluronic acid synthesis promoter of the present invention, but this extract is concentrated or diluted with an appropriate solvent (eg, water or lower alcohol). It can also be used. Preferably, the extract is concentrated, water is added to the concentrate to remove precipitated insolubles, and the obtained filtrate is concentrated or diluted with an appropriate solvent, and used for the hyaluronic acid synthesis promoter of the present invention.
【００１０】本発明のヒアルロン酸合成促進剤の剤型に
は特に制限はなく、化粧品、医薬部外品、医薬品などに
配合して用いることができる。特に、化粧料の形態に調
製して用いるのが好ましい。化粧料の形態としては、洗
顔クリーム、化粧水、乳液などが挙げられる。The dosage form of the hyaluronic acid synthesis promoter of the present invention is not particularly limited, and it can be used by blending it in cosmetics, quasi-drugs, pharmaceuticals and the like. In particular, it is preferable to prepare and use it in the form of a cosmetic. Examples of the form of the cosmetic include a face wash cream, a lotion, an emulsion and the like.
【００１１】本発明のヒアルロン酸合成促進剤中のラベ
ンダー抽出液の配合割合は、ラベンダー抽出液の濃度お
よび剤型によって異なるが、通常は０.００１〜９９重
量％、好ましくは０.０１〜５０重量％の範囲内であ
る。さらに具体的には、後記実施例１に記載のラベンダ
ー抽出液を用いて化粧料を調製する場合、化粧料中のラ
ベンダー抽出液の配合量は、化粧料全量の０.００１〜
１０重量％、好ましくは０.００５〜５重量％、さらに
好ましくは０.０１〜１重量％の範囲内である。The mixing ratio of the lavender extract in the hyaluronic acid synthesis promoter of the present invention varies depending on the concentration and the dosage form of the lavender extract, but is usually 0.001 to 99% by weight, preferably 0.01 to 50% by weight. % By weight. More specifically, when a cosmetic is prepared using the lavender extract described in Example 1 below, the amount of the lavender extract in the cosmetic is 0.001 to 0.001 of the total amount of the cosmetic.
It is in the range of 10% by weight, preferably 0.005 to 5% by weight, more preferably 0.01 to 1% by weight.
【００１２】本発明のヒアルロン酸合成促進剤は、ラベ
ンダー抽出液の他に、上記剤型に調製する際に一般的に
使用される各種成分を含有する。化粧料に調製する場合
に一般的に使用される他の成分は、例えば、油分、界面
活性剤、保湿剤、増粘剤、防腐剤、香料、着色料、薬剤
などである。The hyaluronic acid synthesis promoter of the present invention contains, in addition to the lavender extract, various components generally used in preparing the above-mentioned dosage form. Other ingredients commonly used in preparing cosmetics include, for example, oils, surfactants, humectants, thickeners, preservatives, fragrances, colorings, drugs and the like.
【００１３】[0013]
【実施例】以下に本発明のヒアルロン酸合成促進剤に用
いるラベンダー抽出液の製造、ヒアルロン酸合成促進効
果の測定、ならびに各種化粧料の製造に関する実施例を
挙げ、本発明を更に詳しく説明するが、本発明はこれら
実施例に限定されるものではない。実施例１ ラベンダー抽出液の製造 ラベンダー(高砂薬業)２４０gにエタノール１８００ml
を加え、４０℃の加温下に１０日間静置することによ
り、浸漬抽出を行った。抽出液を濾過し、１３２５mlの
抽出液を得た。この抽出液を６０℃の加温下に減圧濃縮
して乾固した後、水６００mlを加え、４℃にて４８時間
静置した。生成した不溶性の沈殿物を濾過して除去し、
５８０mlの濾液を得た。これに蒸留水５８０mlを加え、
ラベンダー抽出液１１６０mlを得た。EXAMPLES The present invention will be described in more detail below with reference to Examples relating to the production of a lavender extract used for the hyaluronic acid synthesis promoter of the present invention, the measurement of the hyaluronic acid synthesis promoting effect, and the production of various cosmetics. However, the present invention is not limited to these examples. Example 1 Production of Lavender Extract Liquid 1800 ml of ethanol in 240 g of lavender (Takasago Pharmaceutical)
Was added, and the mixture was allowed to stand at a temperature of 40 ° C. for 10 days to perform immersion extraction. The extract was filtered to obtain 1325 ml of extract. The extract was concentrated under reduced pressure while heating at 60 ° C. and dried to dryness. 600 ml of water was added thereto, and the mixture was allowed to stand at 4 ° C. for 48 hours. The insoluble precipitate formed is removed by filtration,
580 ml of filtrate were obtained. Add 580 ml of distilled water to this,
1160 ml of a lavender extract was obtained.
【００１４】実施例２ 正常ヒト皮膚線維芽細胞のヒア
ルロン酸合成能の測定 １０％牛胎児血清含有のＲＩＴＣ８０-７培地(極東製薬
工業株式会社)で継代培養した正常ヒト皮膚線維芽細胞
(ＮＢ１ＲＧＢ)を試験に用いた。この培地を、実施例１
で調製したラベンダー抽出液を最終濃度０.５％となる
ように含有させた０.５％牛胎児血清含有のＲＩＴＣ８
０-７培地に置き換え、５％ＣＯ2下に３７℃で３日間培
養した。次いで、培養上清を回収し、培養上清中のヒア
ルロン酸含量を今成らの方法[Biol.Pharm.Bull., 17(1
2), 1663-1665 (1994)]によって測定した。即ち、回収
した培養上清を、プロテアーゼ処理およびコンドロイチ
ナーゼ処理することによって、ヒアルロン酸をヒアルロ
ン酸不飽和二糖(ΔＤi−ＨＡ)に変換し、その量(ピーク
強度)をＨＰＬＣにて測定した。なお、ラベンダー抽出
液を添加せずに上記と同様に処理して得た結果を対照と
した。これらの測定結果を図１にグラフとして示した。
このグラフから明らかなように、０.５％ラベンダー抽
出液を添加した場合、無添加に比べてヒアルロン酸不飽
和二糖が４３％増えることがわかった。即ち、ラベンダ
ー抽出液は、正常ヒト皮膚線維芽細胞のヒアルロン酸合
成を促進することが明らかになった。 Example 2 Measurement of Hyaluronic Acid Synthesis Ability of Normal Human Skin Fibroblasts Normal human skin fibroblasts subcultured in RITC80-7 medium containing 10% fetal bovine serum (Kyokuto Pharmaceutical Co., Ltd.)
(NB1RGB) was used for the test. This medium was prepared in Example 1
RITC8 containing 0.5% fetal bovine serum containing the lavender extract prepared in the above at a final concentration of 0.5%.
The medium was replaced with 0-7 medium and cultured at 37 ° C. for 3 days under 5% CO 2 . Next, the culture supernatant was collected, and the content of hyaluronic acid in the culture supernatant was determined using the method described in the present [Biol. Pharm. Bull., 17 (1
2), 1663-1665 (1994)]. That is, the recovered culture supernatant was treated with protease and chondroitinase to convert hyaluronic acid into hyaluronic acid unsaturated disaccharide (ΔDi-HA), and the amount (peak intensity) was measured by HPLC. . In addition, the result obtained by treating in the same manner as above without adding the lavender extract was used as a control. These measurement results are shown as a graph in FIG.
As is clear from this graph, when the 0.5% lavender extract was added, the amount of the unsaturated disaccharide of hyaluronic acid was increased by 43% as compared with the case where no lavender extract was added. That is, it was revealed that the lavender extract promoted the synthesis of hyaluronic acid in normal human skin fibroblasts.
【００１５】実施例３ 洗顔クリーム ラベンダー抽出液(実施例１で調製)を有効成分として下
記の洗顔クリームの処方(全１００重量％)に用いる。 Example 3 Facial cleansing cream Lavender extract (prepared in Example 1) is used as an active ingredient in the following facial cleansing cream formulation (100% by weight in total).
【表１】 成分Ａ 重量％ ミリスチン １４.０ ステアリン酸 １２.０ ラウリン酸 ３.５ オレイルアルコール １.５ ヤシ油脂肪酸アミドプロピルベタイン １０.５ 成分Ｂ 重量％ 濃グリセリン １８.０ 水酸化カリウム ７.０ 防腐剤(パラオキシ安息香酸エステル) 適量 精製水 残部 成分Ｃ 重量％ ラベンダー抽出液 ０.５ 香料 ０.２ 成分Ａを加熱溶解し、８０℃に保持する。別に８０℃で
加熱溶解した成分Ｂを成分Ａに加え、充分混合する。撹
拌しながら冷却を行い、５０℃にて成分Ｃを加え、洗顔
クリームを得た。TABLE 1 Ingredient A wt% myristine 14.0 stearic acid 12.0 lauric acid 3.5 oleyl alcohol 1.5 coconut oil fatty acid amidopropyl betaine 10.5 component B wt% concentrated glycerin 18.0 potassium hydroxide 7. 0 Preservative (paraoxybenzoic acid ester) Appropriate amount of purified water Remaining component C weight% lavender extract liquid 0.5 Perfume 0.2 Component A is dissolved by heating and kept at 80 ° C. Separately, the component B heated and dissolved at 80 ° C. is added to the component A and mixed well. The mixture was cooled while stirring, and the component C was added at 50 ° C. to obtain a face wash cream.
【００１６】実施例４ 化粧水 ラベンダー抽出液(実施例１で調製)を有効成分として下
記の化粧水の処方(全１００重量％)に用いる。 Example 4 Lotion A lavender extract (prepared in Example 1) is used as an active ingredient in the following lotion formulation (100% by weight in total).
【表２】 成 分 重量％ 濃グリセリン ４.０ ソルビット液(７０重量％水溶液) ４.０ クエン酸ナトリウム ０.３ ポリオキシエチレン硬化ヒマシ油 ０.５ エタノール １５.０ ラベンダー抽出液 １.０ 香料 ０.０５ クエン酸(pＨ調整剤) 適量 精製水 残部 全成分を室温にて撹拌および混合して均一な溶液とし、
pＨ５.５に調整して化粧水を得た。Table 2 Ingredients % by weight concentrated glycerin 4.0 Sorbit solution (70% by weight aqueous solution) 4.0 Sodium citrate 0.3 Polyoxyethylene hydrogenated castor oil 0.5 Ethanol 15.0 Lavender extract 1.0 Fragrance 0.05 Citric acid (pH adjuster) Appropriate amount of purified water All remaining components are stirred and mixed at room temperature to form a uniform solution.
The pH was adjusted to 5.5 to obtain a lotion.
【００１７】実施例５ 乳液 ラベンダー抽出液(実施例１で調製)を有効成分として下
記の乳液の処方(全１００重量％)に用いる。 Example 5 Emulsion Lavender extract (prepared in Example 1) is used as an active ingredient in the following emulsion formulation (total 100% by weight).
【表３】 成分Ａ 重量％ ショ糖脂肪酸エステル(第一工業製薬Ｓ-１６０) １.０ 濃グリセリン ６.０ カルボキシビニルポリマー ０.０６ 水酸化カリウム ０.０２８ 防腐剤(パラオキシ安息香酸エステル) 適量 精製水 残部 成分Ｂ 重量％ オリブ油 ４.０ ホホバ油 ４.０ 乳酸ミリスチル ２.０ 自己乳化型モノステアリン酸グリセリン １.５ 親油型モノステアリン酸グリセリン １.５ 成分Ｃ 重量％ ラベンダー抽出液 ０.５ 香料 ０.２ 成分Ａを加熱溶解し、８０℃に保持する。別に８０℃で
加熱溶解した成分Ｂを成分Ａに加え、充分混合する。撹
拌しながら冷却を行い、５０℃にて成分Ｃを加え、乳液
を得た。[Table 3] Component A wt% sucrose fatty acid ester (Daiichi Kogyo Seiyaku S-160) 1.0 concentrated glycerin 6.0 carboxyvinyl polymer 0.06 potassium hydroxide 0.028 preservative (paraoxybenzoate) Purified water Remaining component B wt% Olive oil 4.0 Jojoba oil 4.0 Myristyl lactate 2.0 Self-emulsifying glyceryl monostearate 1.5 Lipophilic glyceryl monostearate 1.5 Component C wt% lavender extract 0 0.5 Fragrance 0.2 Heat and dissolve Component A and keep at 80 ° C. Separately, the component B heated and dissolved at 80 ° C. is added to the component A and mixed well. The mixture was cooled while stirring, and the component C was added at 50 ° C. to obtain an emulsion.
【００１８】実施例６ 実地試験 ラベンダー抽出液を、ヒアルロン酸合成促進効果を持つ
物質として洗顔クリーム、化粧水、乳液などの化粧料に
配合し、これらをヒトに対して適用した結果、皮膚に対
する官能的影響は極めて良好であった。 Example 6 Field test Lavender extract was formulated as a substance having a hyaluronic acid synthesis-promoting effect in cosmetics such as face-wash cream, lotion, milky lotion and the like. Effect was very good.
【００１９】[0019]
【発明の効果】本発明のヒアルロン酸合成促進剤を用い
ることにより、皮膚細胞が活性化されて皮膚におけるヒ
アルロン酸量が増加し、その結果、皮膚の老化防止効果
が図られ、肌のシワの発生が予防され、そして滑らかで
しっとりした瑞々しい肌が得られる。EFFECTS OF THE INVENTION By using the hyaluronic acid synthesis promoter of the present invention, the skin cells are activated and the amount of hyaluronic acid in the skin is increased. The occurrence is prevented and smooth, moist and fresh skin is obtained.
【図１】 ラベンダー抽出液のヒアルロン酸合成促進効
果を調べた結果を示すグラフである。FIG. 1 is a graph showing the results of examining the effect of a lavender extract on promoting hyaluronic acid synthesis.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 ＦＩ // Ａ６１Ｋ 7/50 Ａ６１Ｋ 7/50 ──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. 6 Identification code FI // A61K 7/50 A61K 7/50
Claims (2)
するヒアルロン酸合成促進剤。1. A hyaluronic acid synthesis promoter comprising a lavender extract as an active ingredient.
ヒアルロン酸合成促進剤。2. The hyaluronic acid synthesis promoter according to claim 1, which is prepared in the form of a cosmetic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8345101A JPH10182402A (en) | 1996-12-25 | 1996-12-25 | Agent for promoting synthesis of hyaluronic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8345101A JPH10182402A (en) | 1996-12-25 | 1996-12-25 | Agent for promoting synthesis of hyaluronic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH10182402A true JPH10182402A (en) | 1998-07-07 |
Family
ID=18374298
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8345101A Pending JPH10182402A (en) | 1996-12-25 | 1996-12-25 | Agent for promoting synthesis of hyaluronic acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH10182402A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008007412A (en) * | 2006-06-27 | 2008-01-17 | Maruzen Pharmaceut Co Ltd | Involucrin production promoter and epidermal keratinization normalizing agent |
| US7989590B2 (en) | 2005-03-22 | 2011-08-02 | Rohto Pharmaceutical Co., Ltd | Peptides that increase collagen or hyaluronic acid production |
| WO2013046272A1 (en) * | 2011-09-29 | 2013-04-04 | 株式会社 資生堂 | Promoter for expression of clock gene and hyaluronic acid synthase gene |
| JPWO2013046272A1 (en) * | 2011-09-29 | 2015-03-26 | 株式会社 資生堂 | Clock gene and hyaluronic acid synthase gene expression promoter |
| JP2016180007A (en) * | 2016-07-12 | 2016-10-13 | 株式会社 資生堂 | Hyaluronic acid synthase gene expression promoter |
-
1996
- 1996-12-25 JP JP8345101A patent/JPH10182402A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7989590B2 (en) | 2005-03-22 | 2011-08-02 | Rohto Pharmaceutical Co., Ltd | Peptides that increase collagen or hyaluronic acid production |
| JP2008007412A (en) * | 2006-06-27 | 2008-01-17 | Maruzen Pharmaceut Co Ltd | Involucrin production promoter and epidermal keratinization normalizing agent |
| WO2013046272A1 (en) * | 2011-09-29 | 2013-04-04 | 株式会社 資生堂 | Promoter for expression of clock gene and hyaluronic acid synthase gene |
| JPWO2013046272A1 (en) * | 2011-09-29 | 2015-03-26 | 株式会社 資生堂 | Clock gene and hyaluronic acid synthase gene expression promoter |
| US9795811B2 (en) | 2011-09-29 | 2017-10-24 | Shiseido Company, Ltd. | Expression promoting agent for clock gene and hyaluronic acid synthase gene |
| EP2762130B1 (en) * | 2011-09-29 | 2018-03-28 | Shiseido Company, Ltd. | Promoter for expression of clock gene and hyaluronic acid synthase gene |
| JP2016180007A (en) * | 2016-07-12 | 2016-10-13 | 株式会社 資生堂 | Hyaluronic acid synthase gene expression promoter |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0668072B1 (en) | Cosmetic composition containing a plant extracellular matrix extract | |
| EP0727217A2 (en) | Pharmaceutical composition containing god-type ellagitannin as active ingredient | |
| US5955083A (en) | Use of eriobotrya japonica extract, in particular in cosmetics for stimulating glycosaminoglycan synthesis | |
| KR20090056397A (en) | Skin external composition containing Bok herbal extract as an active ingredient | |
| KR102606356B1 (en) | Cosmetic composition for regenerating skin and improving skin wrinkle | |
| JP3202810B2 (en) | Cosmetics | |
| JPH07101871A (en) | Biological hyaluronic acid synthesis promoter | |
| JPH07277939A (en) | Skin external preparation | |
| KR20100092494A (en) | Mixed butyric-formic esters of acid polysaccharides, and their preparation and use as skin cosmetics | |
| JP3249844B2 (en) | Skin cosmetics | |
| EP0531978A2 (en) | Promotor for biological hyaluronic acid synthesis, composition externally applied to the skin and use thereof | |
| JPH09241146A (en) | Skin external agent | |
| JP3172753B2 (en) | Biological hyaluronic acid synthesis promoter | |
| JPH06336422A (en) | External skin preparation | |
| JPH10182402A (en) | Agent for promoting synthesis of hyaluronic acid | |
| KR100295875B1 (en) | Cosmetics Containing Green Bean Extract | |
| KR101662272B1 (en) | Natural cosmetic composition for skin moisturizing comprising Mixed extract of barley and germ and rice bran of black rice and preparation method thereof | |
| JP3522609B2 (en) | Hyaluronic acid production promoter and skin external preparation containing the same | |
| JP3643690B2 (en) | Skin preparation | |
| JPH0967251A (en) | Hyaluronidase inhibitor containing rosmarinic acid as active ingredient | |
| KR20150116504A (en) | A cosmetics composition containing tea tree and solt | |
| JPH11171784A (en) | External preparation for skin | |
| JP3585154B2 (en) | Anti-aging skin external preparation | |
| JP2001253817A (en) | Collagen synthesis promoter and method for producing the same and skin care preparation containing the same | |
| WO1999009945A1 (en) | Use of the rhoeo discolor plant extracts in cosmetics and pharmaceutics, in particular in dermatology |