JPH10274656A - Measurement method of glucose concentration - Google Patents
Measurement method of glucose concentrationInfo
- Publication number
- JPH10274656A JPH10274656A JP9455097A JP9455097A JPH10274656A JP H10274656 A JPH10274656 A JP H10274656A JP 9455097 A JP9455097 A JP 9455097A JP 9455097 A JP9455097 A JP 9455097A JP H10274656 A JPH10274656 A JP H10274656A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- glucose concentration
- measurement
- plasma
- specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 55
- 239000008103 glucose Substances 0.000 title claims abstract description 55
- 238000000691 measurement method Methods 0.000 title claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 51
- 210000004369 blood Anatomy 0.000 claims abstract description 48
- 239000008280 blood Substances 0.000 claims abstract description 48
- 230000003287 optical effect Effects 0.000 claims description 17
- 238000001514 detection method Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 4
- 238000005259 measurement Methods 0.000 abstract description 44
- 238000012937 correction Methods 0.000 abstract description 12
- 238000012545 processing Methods 0.000 abstract description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 14
- 210000000601 blood cell Anatomy 0.000 description 8
- 238000005070 sampling Methods 0.000 description 5
- 238000011088 calibration curve Methods 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 108010015776 Glucose oxidase Proteins 0.000 description 3
- 239000004366 Glucose oxidase Substances 0.000 description 3
- 229940116332 glucose oxidase Drugs 0.000 description 3
- 235000019420 glucose oxidase Nutrition 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000002123 temporal effect Effects 0.000 description 3
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 230000002414 glycolytic effect Effects 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000005534 hematocrit Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【０００１】[0001]
【発明が属する技術分野】臨床検査の分野における生化
学検査として、検体中のグルコース濃度を測定する方法
に関する発明である。更に詳しくは、測定される検体が
血漿であるのか、全血であるのかを判別し、検体に即し
た測定方法を選択する方法及び該方法を用いた測定装置
に関する発明である。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for measuring a glucose concentration in a sample as a biochemical test in the field of clinical test. More specifically, the present invention relates to a method for determining whether a sample to be measured is plasma or whole blood, selecting a measurement method suitable for the sample, and a measurement apparatus using the method.
【０００２】[0002]
【従来の技術】臨床検査では、グルコース濃度を測定す
る時に用いられる検体は、専用の採血管を用いて採血さ
れるが、採血管の中には抗凝固剤と解糖阻止剤が添加さ
れた血液を遠心分離することによって得られる血漿を使
用している。最近では遠心分離された採血管を、直接サ
ンプルラックに入れて、血漿を人がサンプルカップに分
取しなくとも自動分析装置にセットすることによって、
グルコース濃度の測定を行うことができる。2. Description of the Related Art In a clinical test, a sample used for measuring a glucose concentration is collected using a dedicated blood collection tube, and an anticoagulant and a glycolytic inhibitor are added to the blood collection tube. Plasma obtained by centrifuging blood is used. Recently, centrifuged blood collection tubes are placed directly in sample racks, and plasma is set in an automatic analyzer without humans dispensing them into sample cups.
Measurement of glucose concentration can be performed.
【０００３】グルコース濃度の測定は、一定量の血漿中
に含まれるグルコースを、グルコース分解酵素であるグ
ルコースオキシダーゼ（ＧＯＤ）によって分解し、この
時の分解生成物である過酸化水素量或いは分解時に消費
される酸素量を電気化学的に測定し、既存の検量線から
グルコースの濃度を求めている。[0003] In measuring the glucose concentration, glucose contained in a certain amount of plasma is decomposed by glucose oxidase (GOD), which is a glucose decomposing enzyme, and the amount of hydrogen peroxide, which is a decomposition product at this time, or consumed during decomposition. The amount of oxygen thus obtained is electrochemically measured, and the concentration of glucose is determined from an existing calibration curve.
【０００４】グルコース濃度を測定する検体としては、
以前から血漿が用いられてきた。グルコース濃度を測定
する検体は、抗凝固剤あるいは解糖阻止剤が添加された
専用の採血管を用いて患者から採血した血液を入れ、測
定を行う前には必ず遠心分離操作を行うことによって血
球層と血漿層に分離した後、先に述べた通り血漿のみを
分取してから自動分析装置で測定を行うか、最近は自動
分析装置によって血漿のみをサンプリングして測定を行
っていた。本発明では、採血をした後、遠心分離操作を
行わずに血漿層と血球層に分離していない検体のことを
全血と言うが、遠心分離操作を行っていない全血であっ
てもグルコース濃度を求めることができる測定方法が発
明された（特願平８−１３４８６７号）。全血を用いて
グルコース濃度を測定する場合は、全血中の固形成分と
して、赤血球や白血球などの血球成分が混在しているた
め血球成分である血球膜などの影響を受けて、実際の採
取量よりも固形成分量だけ少なくなることによりグルコ
ース濃度は血漿の値よりも低くなってしまう。そこで全
血を用いたグルコース濃度値と、検体のヘマトクリット
値との関係を見出し、その関係から補正式を求めた。こ
れによって全血であっても、補正式を用いて血漿の値に
補正して、血漿中のグルコース濃度値として測定結果を
出力する発明である。よって検体に全血を用いる場合で
あっても血漿を用いた時と同じ値を求めることが可能と
なった訳である。[0004] Samples for measuring glucose concentration include:
Plasma has been used for some time. Samples for measuring glucose concentration are prepared by placing blood collected from a patient using a special blood collection tube to which an anticoagulant or glycolytic inhibitor has been added, and performing a centrifugation operation before measurement. After separation into a plasma layer and a plasma layer, as described above, only the plasma is separated and then measured by an automatic analyzer, or recently, only the plasma is sampled by the automatic analyzer to measure. In the present invention, a sample that has not been separated into a plasma layer and a blood cell layer without centrifugation after blood collection is referred to as whole blood. A measurement method capable of determining the concentration has been invented (Japanese Patent Application No. 8-134687). When measuring glucose concentration using whole blood, the actual blood collection is affected by blood cell components such as red blood cells and white blood cells as solid components in the whole blood. If the amount of the solid component is smaller than the amount, the glucose concentration will be lower than the plasma value. Therefore, the relationship between the glucose concentration value using whole blood and the hematocrit value of the sample was found, and a correction formula was determined from the relationship. This is an invention in which even if it is whole blood, it is corrected to a plasma value using a correction formula, and the measurement result is output as a glucose concentration value in the plasma. Therefore, even when whole blood is used as a sample, the same value as when plasma is used can be obtained.
【０００５】ただし、測定する複数の検体が、全血の検
体と血漿の検体とが混在する場合は、全血の検体と血漿
の検体は測定を行う行程が異なるために、補正式を用い
る全血の検体群と補正式を用いない血漿の検体群をに分
けてから、それぞれの群ごとに測定を行う必要があり、
この時検体群に応じて測定装置の切り替えを行うことも
必要であった。この場合、測定者に対して検体を分ける
手間と装置の切り替えをする手間としての負担を測定者
に掛けることになる。また、測定装置の切り替えを行わ
ずに補正式を用いて測定することも可能ではあるが、こ
の場合は、一次微分法による測定工程に続いて平衡点法
による測定工程が行われるため血漿中のグルコース濃度
を測定する場合は、一次微分法による測定工程のみでグ
ルコース濃度値を求めることが可能であるのに、平衡点
法による測定も行われるため、その分測定時間が長くな
ってしまう。[0005] However, when a plurality of samples to be measured include a whole blood sample and a plasma sample, since the whole blood sample and the plasma sample have different measurement processes, a correction formula using a correction formula is used. It is necessary to divide the blood sample group and the plasma sample group without using the correction formula, and then perform measurement for each group,
At this time, it was necessary to switch the measuring device according to the sample group. In this case, a burden is imposed on the measurer as labor for dividing the sample and for switching the apparatus. It is also possible to measure using a correction formula without switching the measuring device, but in this case, the measurement process by the equilibrium point method is performed following the measurement process by the first derivative method, so that the plasma In the case of measuring the glucose concentration, the glucose concentration value can be obtained only by the measurement step by the first derivative method, but the measurement by the equilibrium point method is also performed, so that the measurement time becomes longer.
【０００６】１検体当たりのグルコース濃度値を求める
ために必要な測定時間は、検体として血漿を用いた場合
には、一次微分法によるグルコース濃度測定値（ＤＩ）
を求めるのみであるため２０秒程度である。検体として
全血を用いた場合は、まず一次微分法によるグルコース
濃度測定値（ＤＩ）を求めた後、引き続き平衡点法によ
るグルコース濃度測定値（ＥＰ）を求めるため、測定時
間が数秒程度余計に必要となり、検体数が多い場合は測
定が終了するまでに長時間にも及んでしまうという問題
があった。[0006] The measurement time required to determine the glucose concentration per sample is, when plasma is used as the sample, the measured glucose concentration (DI) by the first derivative method.
It is only about 20 seconds since the above is only required. When whole blood is used as the specimen, the glucose concentration measurement value (DI) is first determined by the first derivative method, and then the glucose concentration measurement value (EP) is determined by the equilibrium point method. When the number of specimens is large, there is a problem that it takes a long time until the measurement is completed.
【０００７】[0007]
【発明が解決しようとする課題】グルコース濃度を測定
する自動分析装置が、検体として全血であっても血漿で
あっても同等のグルコース濃度値を求めることのできる
装置を用いて、検体中のグルコース濃度を測定するとき
に、測定者が、検体が全血であるのか、血漿であるのか
の判別を行わなくとも、自動分析装置によって自動的に
全血と血漿の判別を行って測定を行う測定効率の良い自
動分析装置が望まれていた。そのためにグルコース濃度
を測定する検体が、自動分析装置によって検体がサンプ
リングされる前に、又はサンプリング時に、血漿である
か全血であるかの判別を電極又は光学的な方法で行った
後、該検体に即した測定方法を自動的に選択して一次微
分法によるグルコース濃度測定値（ＤＩ）を求め、引き
続き平衡点法によるグルコース濃度測定値（ＥＰ）を求
めて、ＤＩ及びＥＰの結果から補正式によって血漿理論
値を求めるか、又は一次微分法によってグルコース濃度
測定値（ＤＩ）を求める。本発明により、検体が全血と
血漿が混在する場合であっても、測定者が検体を分別を
行う必要がなく、検体を自動分析装置にセットするだけ
で検体に応じた測定方法を自動的に選択して測定を行う
ために、測定者に対しては負担がかからず効率的な測定
が行われるため測定時間を短縮することも可能となっ
た。SUMMARY OF THE INVENTION An automatic analyzer for measuring a glucose concentration uses a device capable of obtaining an equivalent glucose concentration value even if the sample is whole blood or plasma. When measuring the glucose concentration, the measurement is performed by automatically discriminating between the whole blood and the plasma by the automatic analyzer, without having to determine whether the sample is whole blood or plasma. An automatic analyzer with high measurement efficiency has been desired. For this purpose, the sample for which the glucose concentration is to be measured, before the sample is sampled by the automatic analyzer or at the time of sampling, after discrimination between plasma or whole blood is performed by an electrode or an optical method, The measurement method suitable for the sample is automatically selected, the glucose concentration measurement value (DI) is obtained by the first derivative method, and the glucose concentration measurement value (EP) is subsequently obtained by the equilibrium point method, and corrected from the DI and EP results. The theoretical plasma value is determined by the formula, or the measured glucose concentration (DI) is determined by the first derivative method. According to the present invention, even when the sample is a mixture of whole blood and plasma, there is no need for the measurer to separate the sample, and the measurement method according to the sample can be automatically performed simply by setting the sample in the automatic analyzer. In order to perform the measurement by selecting, the measurement is performed without any burden on the measurer, so that the measurement time can be shortened.
【０００８】[0008]
【課題を解決するための手段】検体中のグルコース濃度
を測定する時に、自動分析装置によって検体をサンプリ
ングする前か、又はサンプリング時に検体を判別する必
要がある。検体が全血であるのか、血漿であるのかの判
別方法は、電極を用いた判別方法若しくは光学的な判別
方法によって行う。以下に検体の判別する方法として前
者を電極法と称し、後者を光学的検出法と称して説明を
行う。When measuring the glucose concentration in a sample, it is necessary to determine the sample before or at the time of sampling the sample by an automatic analyzer. The method for determining whether the sample is whole blood or plasma is performed by a determination method using electrodes or an optical determination method. Hereinafter, as a method of discriminating a specimen, the former will be referred to as an electrode method, and the latter will be referred to as an optical detection method.
【０００９】電極法による検体の判別について説明す
る。検体の入った容器に対して相対的に移動する電極２
本を用いる。うち１本をサンプリングノズルと兼用とし
ても良い。この電極は検体の判別を行うと共に検体の液
面検知を同時に行う。検体の判別は、検体中に電極を挿
入して電気伝導度の測定を行う。電極が検体に接液した
時における電気伝導度は、固形成分である血球、通常の
場合は血球の大部分が赤血球となる。血球自体は伝導性
が悪いため、血球が混在することによって電気伝導度が
低下する。このため、電極が一定基準以下の電気伝導度
を検出した場合は、検体が全血であると判断することが
できる。一定量の検体を採取して一次微分法によるグル
コース濃度測定値（ＤＩ）を求めた後、更に平衡点法に
よるグルコース濃度測定値（ＥＰ）を求めて、ＤＩ及び
ＥＰの結果から、補正式によってグルコース濃度を求め
る。電極が一定基準以上の電気伝導度を検出した場合
は、血漿と判断することができるため、一定量の検体を
採取して一次微分法によるグルコース濃度測定値（Ｄ
Ｉ）からグルコース濃度を求めれば良い。The determination of a specimen by the electrode method will be described. Electrode 2 that moves relatively to the container containing the sample
Use a book. One of them may be used also as a sampling nozzle. This electrode performs the determination of the sample and simultaneously detects the liquid level of the sample. The determination of the sample is performed by inserting an electrode into the sample and measuring the electrical conductivity. The electrical conductivity when the electrode is in contact with the sample is that the blood cells, which are solid components, are usually red blood cells in most cases. Since blood cells themselves have poor conductivity, the electrical conductivity is reduced by the mixture of blood cells. For this reason, when the electrode detects an electric conductivity that is equal to or less than a certain reference, it can be determined that the sample is whole blood. After a certain amount of sample is collected and the glucose concentration measurement value (DI) is obtained by the first derivative method, the glucose concentration measurement value (EP) is further obtained by the equilibrium point method, and from the results of DI and EP, a correction formula is obtained. Determine the glucose concentration. When the electrode detects an electrical conductivity of a certain level or more, it can be determined that the sample is plasma. Therefore, a certain amount of a sample is collected and a glucose concentration measurement value (D
The glucose concentration may be determined from I).
【００１０】次に光学的検出法について説明する。光源
としてＬＥＤを用いて検体の入った採血管に対して光を
照射する。採血管内部の状態が反映された光信号を、光
センサで捕らえる。捕らえられる信号は反射光、散乱
光、透過光の何れでも良い。この時、採血管中の検体が
存在しない空気層と、検体が存在する検体層との境界
が、光信号の違いとして捕らえることが可能である。更
に検体層においては血漿層と血球層の境界も光信号とし
て捕らえることができるため、採血管中の検体が遠心分
離された血漿であるのか、全血であるのかの判別が可能
となる。Next, an optical detection method will be described. Using a LED as a light source, light is applied to a blood collection tube containing a sample. An optical signal reflecting a state inside the blood collection tube is captured by an optical sensor. The captured signal may be any of reflected light, scattered light, and transmitted light. At this time, the boundary between the air layer in the blood collection tube where the sample does not exist and the sample layer where the sample exists can be grasped as a difference in the optical signal. Further, in the sample layer, the boundary between the plasma layer and the blood cell layer can also be captured as an optical signal, so that it is possible to determine whether the sample in the blood collection tube is centrifuged plasma or whole blood.
【００１１】本発明において、一次微分法とは、グルコ
ース濃度が既知の標準溶液について測定開始後の過酸化
水素電極の出力（電流値）と測定時間との関係を測定
し、それに基づいて出力の時間的変化量（出力の時間に
よる微分値、従って出力速度）の最大値とグルコース濃
度との関係を検量線として予め求めておき、その後グル
コース濃度が未知の検体について過酸化水素電極の出力
の時間的変化を測定し、同様に時間的変化量の最大値を
測定し、その最大値から検量線に基づいてグルコース濃
度を求める方法を言う。In the present invention, the first derivative method refers to measuring the relationship between the output (current value) of a hydrogen peroxide electrode after the start of measurement and the measurement time for a standard solution having a known glucose concentration, and based on the measured value, The relationship between the maximum value of the temporal change (differential value of the output over time, and therefore the output speed) and the glucose concentration is determined in advance as a calibration curve, and then the output time of the hydrogen peroxide electrode is determined for a sample whose glucose concentration is unknown. This is a method of measuring the temporal change, similarly measuring the maximum value of the temporal change amount, and calculating the glucose concentration based on the calibration curve from the maximum value.
【００１２】本発明において、平衡点法とは、グルコー
ス濃度が既知の標準溶液について測定開始後の過酸化水
素電極の出力（電流値）が実質的に一定となるまで測定
を継続し、その一定出力とグルコース濃度との関係を検
量線として予め求めておき、その後、グルコース濃度が
未知の検体について過酸化水素電極の出力を測定し、出
力が一定量となるまで測定を継続し、検量線に基づいて
グルコース濃度を求める方法を言う。In the present invention, the equilibrium point method refers to a method of measuring a standard solution having a known glucose concentration until the output (current value) of the hydrogen peroxide electrode becomes substantially constant after the start of the measurement. The relationship between the output and the glucose concentration is determined in advance as a calibration curve, and then the output of the hydrogen peroxide electrode is measured for a sample whose glucose concentration is unknown, and the measurement is continued until the output becomes a constant amount. This is a method for determining the glucose concentration based on the above.
【００１３】[0013]
（実施形態１）図１に示す通り、サンプルラック（図示
せず）に収納された採血管１に電極とサンプリングノズ
ルが兼用しているノズル電極５と電極４を駆動部７の作
動によって降下させ、採血管１に侵入させて採血管１中
の検体液面２に到達したときに、電極が電気伝導度を検
知し、信号処理部８で伝導度を検出した時に駆動部７を
停止させる指令を出すことにより、電極部の下降を直ち
に終了させる。信号処理部８において、検知した電気伝
導度が一定基準以上の場合には、検体が血漿であると認
識して一次微分法による測定モードを選択して、ノズル
電極５に通じているシリンジ６の作動により一定量の検
体を吸引した後、一次微分法によるグルコース濃度測定
値（ＤＩ）を求め、補正式による補正を行わずにグルコ
ース濃度の結果を出力する。一方電気伝導度が一定基準
以下の場合は、検体が全血であることを信号処理部８で
認識して、一次微分法と平衡点法による測定モードを選
択し、一定量の検体を吸引し、一次微分法によるグルコ
ース濃度測定値（ＤＩ）を求め、続いて平衡点法による
グルコース濃度測定値（ＥＰ）を求めた後に、ＤＩ及び
ＥＰの結果から、補正式によって血漿理論値を求めグル
コース濃度の結果を出力する。(Embodiment 1) As shown in FIG. 1, a nozzle electrode 5 and an electrode 4, which are both used as an electrode and a sampling nozzle, are lowered by an operation of a driving unit 7 in a blood collection tube 1 stored in a sample rack (not shown). A command to stop the driving unit 7 when the electrode detects the electric conductivity when the sample liquid level 2 in the blood collection tube 1 is reached by invading the blood collection tube 1 and the signal processing unit 8 detects the conductivity. To immediately terminate the lowering of the electrode section. When the detected electrical conductivity is equal to or higher than a predetermined reference, the signal processing unit 8 recognizes that the sample is plasma, selects a measurement mode by the first-order differentiation method, and selects the measurement mode of the syringe 6 communicating with the nozzle electrode 5. After a certain amount of sample is aspirated by the operation, a glucose concentration measurement value (DI) is obtained by a first-order differentiation method, and the result of the glucose concentration is output without performing correction by a correction formula. On the other hand, when the electric conductivity is equal to or less than a certain standard, the signal processing unit 8 recognizes that the sample is whole blood, selects a measurement mode by the first derivative method and the equilibrium point method, and aspirates a certain amount of the sample. After measuring the glucose concentration measured by the first derivative method (DI) and then measuring the glucose concentration measured by the equilibrium point method (EP), the theoretical plasma value is calculated from the results of DI and EP by a correction formula to determine the glucose concentration. Outputs the result.
【００１４】ノズル電極５によって検体が一定量採取さ
れた後の反応過程を図４を用いて説明する。セル２１に
は予め所定量の緩衝液がポンプ２４によってバルブ２５
から流入されており、検体はセル２１に吐出することに
よって希釈される。セル２１内にはＧＯＤを固定した過
酸化水素電極２２を有し、セル内での検体希釈を充分に
行わせるためにスターラ２３および撹拌子（図示せず）
により撹拌を行っている。測定が終了すると、ポンプ２
６によりバルブ２７を介して測定液は排出される。グル
コース濃度の測定は、ノズルから検体を吐出した時間を
０として、過酸化水素電極２２からの出力と経過時間と
の関係を求めることにより行う。この時一次微分法、平
衡点法として図６にあるような結果を取り込む。以上の
流れを図３に示す。The reaction process after a certain amount of sample is collected by the nozzle electrode 5 will be described with reference to FIG. A predetermined amount of buffer solution is previously stored in the cell
The sample is diluted by discharging into the cell 21. The cell 21 has a hydrogen peroxide electrode 22 with a fixed GOD, and a stirrer 23 and a stirrer (not shown) for sufficiently diluting the sample in the cell.
Stirring. When the measurement is completed, pump 2
The measurement liquid is discharged by the valve 6 through the valve 27. The measurement of the glucose concentration is performed by setting the time when the sample is ejected from the nozzle to 0 and obtaining the relationship between the output from the hydrogen peroxide electrode 22 and the elapsed time. At this time, the results as shown in FIG. 6 are taken in as the first derivative method and the equilibrium point method. The above flow is shown in FIG.
【００１５】光学的検出方法による検体状態の判別につ
いて図２により説明する。血液検体の入った採血管１は
サンプルラック（図示せず）に収納されている。採血管
がサンプリング位置に到達したとき、光源１１のＬＥＤ
を点灯し、検体の入った採血管１に光を照射する。照射
された光は、採血管の状態を反映した光を反射して、こ
の反射光を光センサ１２へ入力する。採血管の状態を反
映した光とは、空気層と検体層の反射光量の差が認めら
れる光信号であり、検体層においては、血漿層と血球層
の反射光量の差が認められる光信号である。光センサの
情報は制御部に送られて、境界面が１つ（空気層１３と
検体層１６）である全血と境界面が２つ（空気層１３と
血漿層１４と血球層１５）である血漿とを光センサー１
２によって光信号として受光することができるため、光
信号の値から検体を判別して、以後電極法と同様に検体
に応じた工程によってグルコース濃度値を求める。The discrimination of the sample state by the optical detection method will be described with reference to FIG. The blood collection tube 1 containing the blood sample is stored in a sample rack (not shown). When the blood collection tube reaches the sampling position, the LED of the light source 11
Is turned on to irradiate the blood collection tube 1 containing the sample with light. The irradiated light reflects light reflecting the state of the blood collection tube, and inputs the reflected light to the optical sensor 12. The light reflecting the state of the blood collection tube is an optical signal in which a difference in the amount of reflected light between the air layer and the sample layer is recognized.In the sample layer, an optical signal in which a difference between the amount of reflected light between the plasma layer and the blood cell layer is recognized. is there. The information of the optical sensor is sent to the control unit, and the whole blood having one boundary surface (the air layer 13 and the sample layer 16) and two boundary surfaces (the air layer 13, the plasma layer 14, and the blood cell layer 15) are used. Optical sensor 1 with certain plasma
2, the specimen is discriminated from the value of the optical signal, and thereafter, a glucose concentration value is obtained by a process corresponding to the specimen in the same manner as in the electrode method.
【００１６】[0016]
【発明の効果】本発明により、検体が血漿であるのか、
全血であるのかの判別を自動的に行い、検体に応じて最
適な測定方法を自動的に選択して、測定することによっ
て測定時間の短縮化を可能にした。更にグルコース濃度
を測定する前に、測定対象となる検体を選択する必要が
なくなり、測定者の負担が軽減された。According to the present invention, whether the specimen is plasma,
It is possible to automatically determine whether the blood is whole blood, automatically select an optimal measurement method according to the sample, and measure the measurement, thereby shortening the measurement time. Further, there is no need to select a sample to be measured before measuring the glucose concentration, and the burden on the measurer is reduced.
【図１】電極による検体検知の概略図FIG. 1 is a schematic diagram of sample detection by electrodes.
【図２】光学的な検体検知の概略図FIG. 2 is a schematic diagram of optical sample detection.
【図３】測定工程のフローチャートFIG. 3 is a flowchart of a measurement process.
【図４】測定装置における測定部の概略図FIG. 4 is a schematic diagram of a measuring unit in the measuring device.
【図５】ＥＰとＤＩを示す概略図FIG. 5 is a schematic diagram showing EP and DI.
Claims (5)
おいて、該検体が血漿であるのか、全血であるのかの判
別を行い、検体に即した測定方法を選択することを特徴
とするグルコース濃度測定方法。1. A method for measuring glucose concentration in a sample, comprising determining whether the sample is plasma or whole blood, and selecting a measurement method suitable for the sample. Measuring method.
１に記載する測定方法。2. The method according to claim 1, wherein the determination of the sample is performed using an electrode.
請求項１に記載する測定方法。3. The method according to claim 1, wherein the determination of the sample is performed by optical detection.
求項１ないし３のいづれか１つに記載する測定方法。4. The method according to claim 1, wherein the determination of the sample is performed simultaneously with the detection of the liquid level.
の判別を請求項２又は請求項３に記載する方法を用いて
行い、検体に即した測定方法を適宜選択することを特徴
とするグルコース濃度測定装置。5. A method according to claim 2 or 3, wherein the determination is made as to whether the sample is plasma or whole blood, and a measurement method suitable for the sample is appropriately selected. Glucose concentration measuring device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9455097A JPH10274656A (en) | 1997-03-28 | 1997-03-28 | Measurement method of glucose concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9455097A JPH10274656A (en) | 1997-03-28 | 1997-03-28 | Measurement method of glucose concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH10274656A true JPH10274656A (en) | 1998-10-13 |
Family
ID=14113432
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9455097A Pending JPH10274656A (en) | 1997-03-28 | 1997-03-28 | Measurement method of glucose concentration |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH10274656A (en) |
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|---|---|---|---|---|
| WO2006011531A1 (en) * | 2004-07-27 | 2006-02-02 | Mitsubishi Kagaku Iatron, Inc. | Method of auto-discrimination of test sample |
| WO2006104005A1 (en) * | 2005-03-29 | 2006-10-05 | Sysmex Corporation | Method of analyte analysis and analyte analyzer |
| CN1332197C (en) * | 2002-10-31 | 2007-08-15 | 爱科来株式会社 | Analyzing electrode for content density and density analyzer |
| US7776023B2 (en) | 2001-12-12 | 2010-08-17 | Arkray, Inc. | Method and implement for opening hole in soft material |
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1997
- 1997-03-28 JP JP9455097A patent/JPH10274656A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7776023B2 (en) | 2001-12-12 | 2010-08-17 | Arkray, Inc. | Method and implement for opening hole in soft material |
| CN1332197C (en) * | 2002-10-31 | 2007-08-15 | 爱科来株式会社 | Analyzing electrode for content density and density analyzer |
| WO2006011531A1 (en) * | 2004-07-27 | 2006-02-02 | Mitsubishi Kagaku Iatron, Inc. | Method of auto-discrimination of test sample |
| US8557599B2 (en) | 2004-07-27 | 2013-10-15 | Mitsubishi Chemical Medience Corporation | Method for automatic determination of sample |
| WO2006104005A1 (en) * | 2005-03-29 | 2006-10-05 | Sysmex Corporation | Method of analyte analysis and analyte analyzer |
| US7854891B2 (en) | 2005-03-29 | 2010-12-21 | Sysmex Corporation | Method of specimen analysis and specimen analyzer |
| JP4881855B2 (en) * | 2005-03-29 | 2012-02-22 | シスメックス株式会社 | Sample analysis method and sample analyzer |
| US9028756B2 (en) | 2005-03-29 | 2015-05-12 | Sysmex Corporation | Specimen analyzing method and specimen analyzing apparatus |
| US9316583B2 (en) | 2005-03-29 | 2016-04-19 | Sysmex Corporation | Specimen analyzing method and specimen analyzing apparatus |
| US10261016B2 (en) | 2005-03-29 | 2019-04-16 | Sysmex Corporation | Specimen analyzing method and specimen analyzing apparatus |
| US8064061B2 (en) | 2006-03-30 | 2011-11-22 | Sysmex Corporation | Sample analyzer and sample analyzing method |
| RU2522270C2 (en) * | 2010-03-05 | 2014-07-10 | Б. Браун Мельзунген Аг | System and method of control of at least one blood parameter |
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