JPH10295367A - New microorganism belonging to genus bacillus - Google Patents
New microorganism belonging to genus bacillusInfo
- Publication number
- JPH10295367A JPH10295367A JP12015797A JP12015797A JPH10295367A JP H10295367 A JPH10295367 A JP H10295367A JP 12015797 A JP12015797 A JP 12015797A JP 12015797 A JP12015797 A JP 12015797A JP H10295367 A JPH10295367 A JP H10295367A
- Authority
- JP
- Japan
- Prior art keywords
- growth
- bacillus
- microorganism
- nacl
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 32
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 16
- 238000006243 chemical reaction Methods 0.000 claims abstract description 6
- 229920002472 Starch Polymers 0.000 claims abstract description 4
- 239000008107 starch Substances 0.000 claims abstract description 4
- 235000019698 starch Nutrition 0.000 claims abstract description 4
- 102000016938 Catalase Human genes 0.000 claims abstract description 3
- 108010053835 Catalase Proteins 0.000 claims abstract description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims abstract description 3
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 3
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 3
- 108010010803 Gelatin Proteins 0.000 claims abstract description 3
- 229910002651 NO3 Inorganic materials 0.000 claims abstract description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims abstract description 3
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 claims abstract description 3
- 239000002253 acid Substances 0.000 claims abstract description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 3
- 235000013345 egg yolk Nutrition 0.000 claims abstract description 3
- 210000002969 egg yolk Anatomy 0.000 claims abstract description 3
- 239000007789 gas Substances 0.000 claims abstract description 3
- 229920000159 gelatin Polymers 0.000 claims abstract description 3
- 239000008273 gelatin Substances 0.000 claims abstract description 3
- 235000019322 gelatine Nutrition 0.000 claims abstract description 3
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 3
- 238000010438 heat treatment Methods 0.000 claims abstract description 3
- 230000004899 motility Effects 0.000 claims abstract description 3
- 230000007935 neutral effect Effects 0.000 claims abstract description 3
- 239000001301 oxygen Substances 0.000 claims abstract description 3
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 3
- 230000009467 reduction Effects 0.000 claims abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 238000000354 decomposition reaction Methods 0.000 claims description 2
- 230000003834 intracellular effect Effects 0.000 claims description 2
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 abstract description 8
- 239000002184 metal Substances 0.000 abstract description 6
- 229910052751 metal Inorganic materials 0.000 abstract description 6
- 239000002351 wastewater Substances 0.000 abstract description 5
- 238000003794 Gram staining Methods 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 abstract 1
- 210000005056 cell body Anatomy 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 235000019512 sardine Nutrition 0.000 description 3
- 239000005909 Kieselgur Substances 0.000 description 2
- 241001125048 Sardina Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 2
- 238000004332 deodorization Methods 0.000 description 2
- 230000001877 deodorizing effect Effects 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229910001562 pearlite Inorganic materials 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 241000555825 Clupeidae Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 239000002759 woven fabric Substances 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、バチルス（Bacillus)
属に属する新規微生物に関する。BACKGROUND OF THE INVENTION The present invention relates to a Bacillus.
It relates to a novel microorganism belonging to the genus.
【０００２】[0002]
【従来の技術】排水浄化菌としては、各種のバチルス属
微生物が知られている。しかしながら、排水浄化におい
ては、単に浄化に止まらずに、その脱臭や、金属物質の
除去も大切な要素である。2. Description of the Related Art As wastewater purifying bacteria, various Bacillus microorganisms are known. However, in purification of wastewater, not only purification but also deodorization and removal of metal substances are important factors.
【０００３】しかしながら、現在までに知られているバ
チルス属微生物では、これらの全ての要素を満足する微
生物は発見させれおらず、そのような微生物が強く望ま
れていた。However, among the microorganisms of the genus Bacillus known to date, no microorganism satisfying all of these factors has been found, and such microorganisms have been strongly desired.
【０００４】[0004]
【発明が解決しようとする課題】そこで、本発明は、排
水の単なる浄化とともに、その脱臭や金属除去もできる
新規な微生物を提供することを目的とするものである。SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a novel microorganism which can not only purify wastewater but also deodorize and remove metals.
【０００５】[0005]
【課題を解決するための手段】本発明らは、上記のよう
な要素を全て満足することのできる有用な新菌株を求め
て鋭意探索したところ、浄化、脱臭、金属除去の全ての
機能を有する一菌株を土壌から分離することに成功し
た。この一菌株の菌学的性質の試験および分類は、下記
の文献に従い行なった。DISCLOSURE OF THE INVENTION The present invention has been intensively searched for a useful new strain which can satisfy all of the above factors, and has all the functions of purification, deodorization, and metal removal. One strain was successfully isolated from the soil. The test and classification of the mycological properties of this one strain were performed according to the following literature.
【０００６】バージェイズ マニュアル オブ デタ
ーミネティブ バクテリオロジー第９版（1994年）（Be
rgey's Manual of Determinaitive Bacteriology 8th E
dition） バージェイズ マニュアル オブ システマティック
バクテリオロジー第２版（1986年）（Bergey's Manua
l of Systematic Bacteriology 2nd Edition） ザ ジーナス バチルス（1973年）（U.S. Departmen
t of Agriculture)[0006] The Barjay's Manual of Deterministic Bacteriology 9th Edition (1994) (Be
rgey's Manual of Determinaitive Bacteriology 8th E
dition) Bergey's Manua, Manual of Systematic Bacteriology, 2nd Edition (1986)
l of Systematic Bacteriology 2nd Edition) The Genas Bacillus (1973) (US Departmen
t of Agriculture)
【０００７】その結果、その微生物は、耐熱性の胞子を
形成するグラム陽性桿菌であることからバチルスである
と同定されたが、菌体成分の分析結果が、上記の文献に
記載されているいずれの菌種とも一致せず、バチルスに
属する新菌であることが確認された。 当該微生物は、
Bacillus sp. CCC-a（寄託番号：ＦＥＲＭ Ｐ−１６１
７５）として工業技術院生命工学研究所微生物寄託セン
ターに寄託されている。[0007] As a result, the microorganism was identified as Bacillus because it was a gram-positive bacterium that forms heat-resistant spores. Did not correspond to the strain of Bacillus, and was confirmed to be a new bacterium belonging to Bacillus. The microorganism is
Bacillus sp. CCC-a (deposit number: FERM P-161)
75) has been deposited at the Microbial Depositary Center of the Institute of Biotechnology, National Institute of Advanced Industrial Science and Technology.
【０００８】以下、本発明のバチルスに属する新規微生
物の菌学的性質を説明する。Hereinafter, the mycological properties of the novel microorganism belonging to the Bacillus of the present invention will be described.
【０００９】 １．形態 ：桿菌 ２．グラム染色性 ：＋ ３．胞子 ：＋ 形 ：楕円形 位置 ：亜端立〜中立 胞子嚢 ：非膨出 ４．運動性 ：＋ ５．酸素に対する態度 ：好気性 ６．カタラーゼ ：＋ ７．５℃での成育 ：＋ ８．１０℃での成育 ：＋ ９．３０℃での成育 ：＋ １０．４０℃での成育 ：− １１．５０℃での成育 ：− １２．菌体内ＤＮＡのＧＣ含量 ：４０ｍｏｌ％ １３．嫌気下での成育 ：− １４．Ｖ−Ｐ反応 ：− １５．Ｖ−ＰブロスのｐＨ ：６．１ ６以下 ：− ７以上 ：− １６．グリコースからの酸の生成 ：＋ １７．グリコースからのガスの生成 ：＋ １８．ゼラチンの液化 ：＋ １９．デンプンの分解 ：− ２０．クエン酸塩の利用 ：− ２１．プロピオン酸塩の利用 ：− ２２．卵黄反応 ：− ２３．硝酸塩の還元 ：− ２４．ｐＨ６．８での成育 ：− （ニュートリエントブロス） ２５．ｐＨ５．７での成育 ：− ２６．２％Nacl存在下での成育 ：＋ ２７．５％Nacl存在下での成育 ：＋ ２８．７％Nacl存在下での成育 ：＋ ２９．１０％Nacl存在下での成育 ：＋ ３０．胞子の耐熱性 ：＋ （８０℃、１０分間の加熱処理後の生菌状態）[0009] 1. Form: Bacillus 2. Gram stainability: +3. 3. Spores: + Shape: Oval Position: Sub-neutral to neutral Spores: Non-bulging Motility: +5. 5. Attitude to oxygen: aerobic Catalase: + 7.5 ° C. growth: + 8.10 ° C. growth: + 9.30 ° C. growth: + 10.40 ° C. growth: − 11.50 ° C. growth: −12. 12. GC content of intracellular DNA: 40 mol% Growing under anaerobic condition: -14. VP reaction: -15. PH of VP broth: 6.16 or less: -7 or more: -16. Generation of acid from glucose: +17. Generation of gas from glucose: +18. Liquefaction of gelatin: +19. Decomposition of starch: -20. Utilization of citrate: -21. Use of propionate:-22. Egg yolk reaction: -23. Reduction of nitrate:-24. Growth at pH 6.8:-(nutrient broth) 25. Growth at pH 5.7:-Growth in the presence of 26.2% Nacl: + Growth in the presence of 27.5% Nacl: + Growth in the presence of 28.7% Nacl: + 29.10% Nacl Growth under: +30. Heat resistance of spores: + (live bacteria after heat treatment at 80 ° C for 10 minutes)
【００１０】本発明の新規微生物は、一般栄養培地であ
るならいかなる培地でも良好に成育する。[0010] The novel microorganism of the present invention grows well in any nutrient medium.
【００１１】培養は、好ましくは５〜３０℃程度で良好
に増殖する。The culture preferably grows well at about 5 to 30 ° C.
【００１２】本発明の新規微生物は、水や液体培地に分
散させ分散液の状態で各種用途に用いることができる
が、好ましくは微生物群を担体に固定し、取扱を容易に
した担体固定化微生物群として用いる。The novel microorganism of the present invention can be dispersed in water or a liquid medium and used in various applications in the form of a dispersion. Preferably, the microorganisms are immobilized on a carrier to facilitate handling. Use as a group.
【００１３】微生物群を固定する担体としては、微生物
の保持容量が大きく、また微生物の活性化が容易である
ことがこのましい。It is preferable that the carrier for immobilizing the microorganisms has a large holding capacity for the microorganisms and easy activation of the microorganisms.
【００１４】担体としては、岩石（例えば、真珠岩、珪
藻土）またはその粉砕物、砂利、砂、プラスチックス、
セラミックス（例えば、アルミナ、シリカ、天然ゼオラ
イト、合成ゼオライト）、タルク等も好ましく、特に、
多孔質セラミックス、多孔質プラスチックスのような連
続通気孔を有する多孔質材料が好ましい。例えば、真珠
岩の粉砕物を高温高圧で保持し熱処理した後、急激に減
圧して多孔質にした微細粉（商品名：パーライト）は、
菌類を着生させる性質があるため、好適である。このよ
うな多孔質材料において、通気孔の径は、２〜１０μｍ
程度であることが好ましい。また、このような担体の性
状としては、塊状物、粒体、粉体、微粉末またはいた状
物、針状物等いかなるものでもよいが、好ましくは平均
粒径が２ｍｍ以下、特に５０μｍ〜１ｍｍ程度の粉粒体
がよい。なお、このような担体固定化微生物群は、必要
に応じて、布やネット等の通水性や通気性のよい容器に
収納して使用してもよい。As the carrier, rock (eg, perlite, diatomaceous earth) or a crushed product thereof, gravel, sand, plastics,
Ceramics (eg, alumina, silica, natural zeolites, synthetic zeolites), talc, and the like are also preferable.
A porous material having continuous air holes such as porous ceramics and porous plastics is preferable. For example, a fine powder (trade name: pearlite), which is obtained by holding a pearlite pulverized material at high temperature and pressure and heat-treating it and then rapidly reducing the pressure to make it porous,
It is suitable because it has a property of growing fungi. In such a porous material, the diameter of the vent is 2 to 10 μm.
It is preferred that it is about. In addition, the properties of such a carrier may be any such as a lump, a granule, a powder, a fine powder or a powder, a needle, and the like. Good granularity is good. In addition, such a carrier-immobilized microorganism group may be stored and used in a container having good water permeability or air permeability such as a cloth or a net, if necessary.
【００１５】上記担体としては、これらの他、リボン状
やシート状の織布や不織布を用いることができる。As the carrier, other than these, a woven or nonwoven fabric in the form of a ribbon or sheet can be used.
【００１６】このような担体に微生物群を担持させるに
は、担体と本発明の微生物群分散液とを混合した後、乾
燥すればよいが、担体で直接微生物群を培養してもよ
い。In order to carry the microorganisms on such a carrier, the carrier and the dispersion of the microorganisms of the present invention may be mixed and then dried, but the microorganisms may be directly cultured on the carrier.
【００１７】担体の微生物群の担持量は、担持させると
きの条件によっても異なるが、好ましくは、５個〜２０
０億個／ｃｍ3 、好ましくは１０個〜１００億個／ｃｍ
3 である。The amount of the microorganisms to be carried on the carrier varies depending on the conditions at which the microorganisms are carried.
100 million pieces / cm 3 , preferably 10 to 10 billion pieces / cm 3
3
【００１８】本発明の担体固定化微生物群は、排水の浄
化に有効である。また、本発明の新規微生物は、養豚
所、公衆便所等に用いて、脱臭効果が確認された。さら
に、本発明の新規微生物においては、リン等の金属の回
収機能が確認された。さらにまた、土壌の改質にもちい
ても効果が確認された。The carrier-immobilized microorganisms of the present invention are effective for purifying wastewater. In addition, the novel microorganism of the present invention was used in pig farms, public toilets and the like, and its deodorizing effect was confirmed. Furthermore, in the novel microorganism of the present invention, the function of recovering metals such as phosphorus was confirmed. Furthermore, the effect was confirmed using soil modification.
【００１９】なお、本発明の新規微生物は、マウスによ
る経口投与試験によりその安全性が確認された。The safety of the novel microorganism of the present invention was confirmed by an oral administration test using mice.
【００２０】[0020]
【実施例】次に、本発明の実施例について説明する。ま
ず、次のような組成の培地を作成した。Next, an embodiment of the present invention will be described. First, a medium having the following composition was prepared.
【００２１】培地 ペプトン ５ｇ 肉エキス ５ｇ ＮａＣｌ ２．５ｇ 水 ５００ｍｌ（ｐＨ７．０） 以上の組成の培地を、１０００ｍｌ容器にいれ、これに
Bacillus sp. CCC-aを接種し、よく撹拌した後、静置し
て、３０℃で３日間培養した。Medium Peptone 5 g Meat extract 5 g NaCl 2.5 g Water 500 ml (pH 7.0) A medium having a composition of not less than 1000 ml is put in a 1000 ml container, and
Bacillus sp. CCC-a was inoculated, stirred well, allowed to stand, and cultured at 30 ° C. for 3 days.
【００２２】得られた培養液を遠心分離機かけ、菌体を
分離し、これをよく洗浄し、担体に付着させてパウダー
とした。担体としては珪藻土焼いて、粉砕したものを用
いた。The obtained culture was centrifuged to separate the cells, which were thoroughly washed and adhered to a carrier to obtain a powder. As the carrier, diatomaceous earth burned and pulverized was used.
【００２３】このパウダー状のBacillus sp. CCC-aを、
計量対象へ接種してのＣＯＤ、全窒素量および全リン量
の経時変化を計量した。試験方法は、ＣＯＤはＪＩＳ
０１０２−１７、全窒素量はＪＩＳ ０１０２−４５．
２、そして全リン量ＪＩＳ０１０２−４６．２に従って
おこなった。計量対象としては、海水での牛乳１００倍
液に、１リットル当たり０．２ｇの澱粉を加えたものを
用いた。そのままのものを比較例とし、これにBacillus
sp. CCC-aを接種したものを実施例とした。試験は、２
０℃に保って行なった。また、各量の測定にあたって
は、計量対象液を２時間静置し、その上澄み液を試験液
とした。This powdery Bacillus sp. CCC-a is
The time-dependent changes in COD, total nitrogen amount and total phosphorus amount inoculated to the measurement object were measured. The test method is JIS for COD
0102-17, total nitrogen amount is JIS 0102-45.
2, and the total phosphorus amount was carried out in accordance with JIS0102-46.2. As a measurement target, a solution obtained by adding 0.2 g of starch per liter to a 100-fold solution of milk in seawater was used. The Bacillus was used as a comparative example.
An example inoculated with sp. CCC-a was used as an example. Test 2
This was carried out at 0 ° C. In measuring each amount, the liquid to be measured was allowed to stand for 2 hours, and the supernatant was used as a test liquid.
【００２４】試験の結果を表１に示した。なお、試験
は、それぞれサンプルを２つずつ作製し、その平均を測
定値とした。Table 1 shows the results of the test. In the test, two samples were prepared, and the average was used as the measured value.
【００２５】[0025]
【表１】 [Table 1]
【００２６】表１から分かるように、本発明の新規微生
物であるBacillus sp. CCC-aは、水の浄化機能を有し、
金属を除去する作用があることが判明した。さらに、１
本のままの腐った鰯にBacillus sp. CCC-aのパウダーを
振りかけ２時間経過後にその臭いをかいだところ、明ら
かに臭気が減少していた。また、新鮮な鰯にBacilluss
p. CCC-aを振りかけ、２５℃で保管したところ４８時間
を経過しても腐臭はしなかった。一方、Bacillus sp. C
CC-aを振りかけずにそのままの状態で２５℃で保管した
鰯は、２４時間経過でかなり臭いがきつくなった。As can be seen from Table 1, the novel microorganism of the present invention, Bacillus sp. CCC-a, has a water purification function,
It has been found that there is an action of removing metal. In addition, 1
Bacillus sp. CCC-a powder was sprinkled on the rotten sardine as it was and the odor was smelled after 2 hours, and the odor was clearly reduced. Also, fresh sardines in Bacilluss
When sprinkled with p. CCC-a and stored at 25 ° C., no odor was found even after 48 hours. On the other hand, Bacillus sp. C
The sardine stored at 25 ° C. without sprinkling with CC-a became quite odorful after 24 hours.
【００２７】このように、本発明のBacillus sp. CCC-a
は、脱臭作用および鮮度保存作用も備えている。Thus, the Bacillus sp. CCC-a of the present invention
Also has a deodorizing action and a freshness preserving action.
Claims (1)
属する新規微生物。 １．形態 ：桿菌 ２．グラム染色性 ：＋ ３．胞子 ：＋ 形 ：楕円形 位置 ：亜端立〜中立 胞子嚢 ：非膨出 ４．運動性 ：＋ ５．酸素に対する態度 ：好気性 ６．カタラーゼ ：＋ ７．５℃での成育 ：＋ ８．１０℃での成育 ：＋ ９．３０℃での成育 ：＋ １０．４０℃での成育 ：− １１．５０℃での成育 ：− １２．菌体内ＤＮＡのＧＣ含量 ：４０ｍｏｌ％ １３．嫌気下での成育 ：− １４．Ｖ−Ｐ反応 ：− １５．Ｖ−ＰブロスのｐＨ ：６．１ ６以下 ：− ７以上 ：− １６．グリコースからの酸の生成 ：＋ １７．グリコースからのガスの生成 ：＋ １８．ゼラチンの液化 ：＋ １９．デンプンの分解 ：− ２０．クエン酸塩の利用 ：− ２１．プロピオン酸塩の利用 ：− ２２．卵黄反応 ：− ２３．硝酸塩の還元 ：− ２４．ｐＨ６．８での成育 ：− （ニュートリエントブロス） ２５．ｐＨ５．７での成育 ：− ２６．２％Nacl存在下での成育 ：＋ ２７．５％Nacl存在下での成育 ：＋ ２８．７％Nacl存在下での成育 ：＋ ２９．１０％Nacl存在下での成育 ：＋ ３０．胞子の耐熱性 ：＋ （８０℃、１０分間の加熱処理後の生菌状態）1. A novel microorganism belonging to the genus Bacillus having the following mycological properties. 1. Form: Bacillus 2. Gram stainability: +3. 3. Spores: + Shape: Oval Position: Sub-neutral to neutral Spores: Non-bulging Motility: +5. 5. Attitude to oxygen: aerobic Catalase: + 7.5 ° C. growth: + 8.10 ° C. growth: + 9.30 ° C. growth: + 10.40 ° C. growth: − 11.50 ° C. growth: −12. 12. GC content of intracellular DNA: 40 mol% Growing under anaerobic condition: -14. VP reaction: -15. PH of VP broth: 6.16 or less: -7 or more: -16. Generation of acid from glucose: +17. Generation of gas from glucose: +18. Liquefaction of gelatin: +19. Decomposition of starch: -20. Utilization of citrate: -21. Use of propionate:-22. Egg yolk reaction: -23. Reduction of nitrate:-24. Growth at pH 6.8:-(nutrient broth) 25. Growth at pH 5.7:-Growth in the presence of 26.2% Nacl: + Growth in the presence of 27.5% Nacl: + Growth in the presence of 28.7% Nacl: + 29.10% Nacl Growth under: +30. Heat resistance of spores: + (live bacteria after heat treatment at 80 ° C for 10 minutes)
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12015797A JP3769098B2 (en) | 1997-04-23 | 1997-04-23 | New microorganisms belonging to the genus Bacillus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12015797A JP3769098B2 (en) | 1997-04-23 | 1997-04-23 | New microorganisms belonging to the genus Bacillus |
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| Publication Number | Publication Date |
|---|---|
| JPH10295367A true JPH10295367A (en) | 1998-11-10 |
| JP3769098B2 JP3769098B2 (en) | 2006-04-19 |
Family
ID=14779372
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12015797A Expired - Lifetime JP3769098B2 (en) | 1997-04-23 | 1997-04-23 | New microorganisms belonging to the genus Bacillus |
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| Country | Link |
|---|---|
| JP (1) | JP3769098B2 (en) |
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| JP2003080290A (en) * | 2001-09-07 | 2003-03-18 | Tokyo Baiotsukusu:Kk | Drainage stain preventive agent and method for preventing drainage stain using the same |
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|---|---|---|---|---|
| JP2003080290A (en) * | 2001-09-07 | 2003-03-18 | Tokyo Baiotsukusu:Kk | Drainage stain preventive agent and method for preventing drainage stain using the same |
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| JPWO2016031804A1 (en) * | 2014-08-25 | 2017-06-08 | 株式会社日本環境科学研究所 | Composition, carrier, wastewater treatment system, wastewater treatment method, deodorization method, and batch-type wastewater treatment method |
| US10052399B2 (en) | 2014-08-25 | 2018-08-21 | Japan Environmental Science Company | Composition, support, wastewater treatment system, wastewater treating method, deodorization method, and batch wastewater treating method |
| CN113755405A (en) * | 2021-10-15 | 2021-12-07 | 南宁师范大学 | Bacillus bacterium F12 and its application |
| CN113755405B (en) * | 2021-10-15 | 2023-06-20 | 南宁师范大学 | Bacillus bacterium F12 and its application |
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