JPH1138009A - Automatic analyzer - Google Patents

Abstract

(57) [Abstract] [Purpose] By stirring a particle solution so that particles are not uniformly dispersed and settled by rotating a particle bin, it is not necessary to stir the particle solution with a conventional stirring rod. Provided is an automatic analyzer that eliminates the possibility of contamination with other kinds of solutions (contamination). [Constitution] An automatic analyzer that rotates a reaction tube turret supporting a large number of reaction tubes, sequentially supplies particles and dispenses a labeling reagent to each reaction tube, and performs automatic analysis. It is characterized in that a particle bin for storing the contained particle solution is rotated, and the particle solution is stirred so that the particles are uniformly diffused.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for effectively stirring a particle solution by rotating a particle bin for storing the particle solution containing the particles, thereby effectively preventing the particles from settling in the particle solution. The present invention relates to an automatic analyzer capable of performing the above.

[0002]

2. Related Art As an automatic analyzer according to the present invention, for example, an automatic immunoassay for measuring a specific substance in a sample by an antigen-antibody reaction is known.

As the immunoassay using the antigen-antibody reaction, a non-competitive sandwich method and a competitive method of a one-step method and a two-step method are known.

A method of measuring the amount of antigen contained in a sample such as blood collected from a patient as a sample by a one-step non-competitive sandwich method will be described. For example, an antibody bound to an insoluble substance (solid phase) such as an inner wall or a particle (particle) of a synthetic resin container (hereinafter referred to as “solid phase antibody”)
A sample to be measured is added to a reaction tube to which an antibody to which a labeling substance such as a radioactive substance, a fluorescent substance, oxygen, or the like is bound (hereinafter, referred to as a “labeled antibody”) is added.

Thus, in the reaction tube, the antigen contained in the sample reacts with the solid-phase antibody by an antigen-antibody reaction (immune reaction) to form an antigen-antibody complex, and at the same time, the antigen-antibody complex is labeled. The antibody is also conjugated to form a conjugate in which the three components of solid phase antibody-antigen-labeled antibody are sandwiched. In this way, the label of the labeled antibody is bound to the solid phase using the antigen in the sample as a mediator.

Next, extra labeled antibodies other than the label bound to the solid phase and not binding to the antigen added to the reaction tube, antibody components not involved in the immune reaction, and the like are removed. An operation of separation (hereinafter referred to as “B / F separation”) is performed, and finally, the amount of the label proportional to the amount of the antigen bound to the solid phase is quantitatively measured by a physical or chemical method utilizing the properties of the label. Then, the antigen concentration in the sample is determined.

On the other hand, in the non-competitive sandwich method of the two-step method, first, a sample is added to a reaction tube to which only a solid-phase antibody has been previously added to cause a first reaction, and then a labeled antigen is added. This is a method in which a second reaction is performed to perform measurement.

In addition to the non-competitive sandwich method, a competitive method in which an antigen (generally referred to as a “labeled antigen”) previously labeled with a labeling substance and the antigen in a sample competitively reacts with the solid phase antibody described above. Is also known.

In such an immunoassay, when particles (a ferrite coated with plastic) are used as insoluble solids (solid phase), the particles are suspended in a solution in order to uniformly supply the particles. It is necessary to supply the reaction tube to the reaction tube in a state where it is not inserted. However, since particles have a specific gravity greater than that of water, it was necessary to stir the particle solution in the particle bin so that the particles would not uniformly diffuse and settle in the solution.

As a means for stirring the particle solution in the particle bin, a method of inserting a stirring rod into the particle bin and stirring the particle solution is adopted. In the case of using this stir bar, when the type of the particle solution in the particle bin is changed, if the stir bar is used as it is, contamination of another solution (contamination) occurs. Therefore, it was necessary to wash the stirring bar every time the type of the solution changed. This cleaning process becomes more complicated in steps, and the cleaning mechanism also needs to be automated, resulting in a problem that the mechanism becomes complicated.

Further, although the particle solution is expensive, it is discarded while leaving a considerable amount of unsuctionable amount for sucking the particle solution while holding the particle bin in an upright state. Was quite uneconomical.

[0012]

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above-mentioned problems, and is intended to stir a particle solution by rotating a particle bin so that the particles are not uniformly dispersed and settled. It is an object of the present invention to provide an automatic analyzer that eliminates the need for a conventional stirring process of a particle solution using a stirring rod and eliminates the possibility of mixing other types of solutions (contamination).

Another object of the present invention is to provide an automatic analyzer capable of reducing the amount of waste of an expensive particle solution by minimizing the incapable suction amount of the particle solution and reducing the cost.

[0014]

In order to solve the above problems, an automatic analyzer according to the present invention rotates a reaction tube turret supporting a large number of reaction tubes and supplies particles to each reaction tube. An automatic analyzer for sequentially performing dispensing of a labeling reagent and performing an automatic analysis, in which a particle bin for storing a particle solution containing particles is rotated, and the particle solution is stirred so that the particles do not settle. It is characterized by.

As a mode of rotation of the particle bin, there is provided reagent holding means for locking an upper portion of the particle bin and holding the lower portion in a rotatable state. It is preferable that the lower part of the particle bin be rotated as a fulcrum.

The particle bin is rotated in a state where it is always inclined, so that the particle solution located on the inner bottom peripheral edge of the particle bin can be sucked. There is an advantage that it can be minimized.

With respect to the structure for rotating the lower part of the particle bin, the reagent bin is inserted into a reagent holding means and a fitting hole provided below the reagent holding means and formed in a peripheral part. A stirring table,
An agitating shaft formed by projecting an eccentric pin at an upper end portion and connecting the eccentric pin to a central lower surface of the agitation table via a bearing; and a first driving means for rotating the agitation shaft. By rotating the shaft, the eccentric pin eccentrically moves and the stirring table horizontally rotates, whereby the lower part of the particle bin can be rotated with the upper locking part of the particle bin as a fulcrum.

A plurality of particle bins may be held in the reagent holding means, and the plurality of particle bins may be rotated and used sequentially. In this case, in addition to the above-described configuration in which the lower portion of the particle bin is rotated, a rotating plate provided below the stirring table, and a rocking connection unit that connects the stirring table and the rotating plate so as to be horizontally rotatable with each other. A rotating pulley rotatably attached to the stirring shaft and connected to the rotating plate; and a second driving unit for rotating the rotating pulley, wherein the reagent holding unit includes a plurality of particle bins. By holding and rotating the rotary pulley, a plurality of particle bins are sequentially positioned at the particle supply position.

The reagent holding means may comprise a reagent holder having an upper portion of the particle bin detachably locked and a lower portion rotatable, and a reagent table for detachably holding the reagent holder. .

The reagent holder may have a structure capable of simultaneously holding a particle bin and a labeled reagent bin. In this case, the particle bin removably locks the upper portions of the particle bin and the labeling reagent bin, and allows the stirrer table to freely insert the labeling reagent bin in addition to the insertion hole for inserting the particle bin. A hole is formed, and the lower part of the labeling reagent bin is loosely inserted into the play insertion hole. When the stirring table is horizontally rotated, the particle bin is rotated with the movement of the stirring table. The bottle does not come into contact with the stirring table during the movement of the stirring table, and can maintain a stationary state.

The swing connection means is not particularly limited as long as the stirring table and the rotating plate are connected to each other so as to be able to rotate horizontally, but a preferred construction is to have a connection rod, The upper part is pivotally connected to the lower surface of the stirring table and the lower part of the connecting rod is pivotally connected to the upper surface of the rotating plate, respectively. What is necessary is just to connect the said reagent table with each other.

It is necessary to use the particle solution and the labeling reagent at a predetermined temperature, and it is preferable that the automatic analyzer of the present invention is provided with a heat retaining means. For example, the particle bottle and the labeling reagent bottle are kept warm. Connecting a heat retaining case to the rotating pulley below the rotating plate,
The heat retaining case can be configured to be rotatable together with the rotating plate.

If a bar code indicating measurement items, operating conditions, and the like is attached to the front surface of the reagent holder, there is an advantage that the measurement items, operating conditions, and the like in measurement can be automatically read at the same time.

[0024]

DESCRIPTION OF THE PREFERRED EMBODIMENTS One embodiment of the present invention will be described below with reference to the accompanying drawings.

FIG. 1 is a top view showing one embodiment of the automatic immunoassay apparatus according to the present invention. FIG. 2 is a front view of FIG. FIG. 3 is an enlarged top view showing a part of a reagent table mechanism of the automatic immunoassay apparatus of the present invention.
FIG. 4 is a sectional view of FIG. FIG. 5 is an enlarged perspective view of the reagent holder that locks the particle bin and the labeled reagent bin. FIG. 6 is an enlarged perspective view showing a state where the locking of the particle bin and the labeling reagent bottle is released from the state of FIG. 7A and 7B are views showing a state in which the particle bin is horizontally rotated. FIG. 7A is a top view, and FIG.
7 (c) is a sectional view taken along the line CC and FIG. 7 (d).
FIG. 4 is a sectional view taken along line DD. FIG. 8 is an explanatory cross-sectional view showing a connection state between the stirring table and the rotary plate.

In FIG. 1, reference numeral 12 denotes an automatic analyzer according to the present invention, specifically, an automatic immunoassay apparatus, which has a reaction tube turret 14 which rotates intermittently at a predetermined speed. At the periphery of the upper surface of the reaction tube turret 14, there is formed a reaction tube holding portion 18 in which a plurality of reaction tube holders 16 are arranged in two rows, an inner row and an outer row. The number of the reaction tube holders 16 provided on the upper surface of the reaction tube turret 14 may be appropriately set according to the mode of the reaction, but in the example of FIG. An example in which a reaction tube holder 14 is installed is shown.

Reference numeral 20 denotes a reaction tube rack, which includes a plurality of receiving portions 21.
(In the example of FIG. 1, a case where two reaction tube racks are installed is shown). Reference numeral 22 denotes a reaction tube transfer means, which has a reaction tube holding portion 22a at a lower portion, and transfers the reaction tube held in the receiving portion 21 of the reaction tube rack 20 to the reaction tube holder 16, and The desired number of reaction tube holders 16 are transferred to another reaction tube holder 16 while skipping the desired number of reaction tube holders 16.

In this case, the reaction tubes held in the inner or outer rows of the reaction tube holders 16 can be transferred to the same circumference of the reaction tube holders 16, or the other circumference of the reaction tube holders 16 can be transferred. Can also be transferred to The reaction tube transfer means 22 performs a reaction tube supply operation of supplying a reaction tube to the reaction tube holding unit 18 and a reaction tube discarding operation of taking out a measured reaction tube from the reaction tube holding unit 18 and discarding it.

Reference numeral 26 denotes particle supply means for supplying particles to the reaction tube, and a particle nozzle 26a is provided below the particle supply means. Reference numeral 28 denotes a reagent dispensing means for dispensing a labeling reagent into the reaction tube, and a reagent nozzle 28a is provided at a lower portion thereof. Reference numeral 30 denotes a cleaning unit for performing B / F separation. Reference numeral 32 denotes a measuring reagent dispensing means for dispensing the measuring reagent into the reaction tube. Reference numeral 34 denotes a measuring means for measuring the amount of the label in the reaction solution after the reaction in the reaction tube.

A reagent table mechanism 36 has a reagent table 37. The reagent table 37 has a mounting hole 3
7a and 37a are drilled, and the mounting holes 37a and 37a are
Reagent holders 38, 38 are removably mounted via a. A particle bin 40 and a labeled reagent bin 42 are detachably attached to the reagent holders 38, 38, respectively.

Reference numeral 44 denotes a substrate, on which a stirring motor 46 as first driving means is provided via a support 48. The first drive pulley 50 is directly connected to the stirring motor 46.

An agitating shaft 52 is provided at its upper end with an eccentric pin 54 eccentrically projecting upward from the center of the upper surface of the agitating shaft (FIGS. 4 and 8). The upper end of the eccentric pin 54 is rotatably attached via a bearing 58 to the center of a stirring table 56 provided with a gap on the lower surface side of the reagent table 37 (FIG. 8).

On the stirring table 56, the particle bin 40 into which the lower part of the particle bin 40 is inserted is inserted.
Insertion hole 57 having a diameter slightly larger than the diameter of the lower part of
And a diameter larger than the diameter of the lower part of the labeling reagent bin 42 into which the lower part of the labeling reagent bin 42 is inserted (as will be described later, the stirring table 56 is caused to move horizontally. And the labeling reagent bottle 42 must have a diameter that does not make contact with the labeling reagent bottle 42).
9 are provided.

A first pulley 60 is fixed to the lower end of the stirring shaft 52. The first driving pulley 50 and the first pulley 60 are connected by a first timing belt 62. When the first driving pulley 50 rotates,
The first pulley 60 is transmitted to the first pulley 60, and rotates with the stirring shaft 52.

A fixed sleeve 64 is provided so as to rotatably cover the outer peripheral surface of the stirring shaft 52. The fixed sleeve 64 is supported by a stirrer motor 46 and a table rotation motor 7 serving as a second driving means via a support plate 66.
0.

A second drive pulley 72 is directly connected to the table rotation motor 70. 74 is the fixed sleeve 64
Is a rotary pulley rotatably provided on the outer peripheral surface of the motor. The second drive pulley 72 and the rotary pulley 74 are connected by a second timing belt 76. 77 is a column for supporting the table rotation motor 70 on the substrate 44.

A central portion of a cup-shaped rotary plate 78 provided below the stirring table 56 is opened, and is connected to the upper surface of the rotary pulley 74 through the opening.
Reference numeral 80 denotes a heat insulation case for keeping the particle bin 40 and the labeling reagent bin 42 warm. The heat retaining case 80 is supported and installed by supporting legs 68, 68 erected on the support plate 66 so as to be positioned below the reagent table so as to surround the rotating plate 78 from below and outside. I have. A heat insulating material 82 is attached to an outer peripheral surface of the heat insulating case 80.

The rotating plate 78 and the reagent table 3
7 are connected to each other by a connection portion 84. Further, the rotating plate 78 and the stirring table 56 are connected to each other via swing connection means 86 so as to be horizontally rotatable.

As shown in FIG. 8, the swing connection means 86
Has a connecting rod 88. The upper and lower portions of the connection rod 88 are pivotally connected to spherical joints 90 and 92 provided on the lower surface of the stirring table 56 and the upper surface of the rotary plate 78, respectively.

When the second drive pulley 72 makes a half turn,
The rotation is transmitted to the rotation pulley 74, the rotation plate 78 and the heat retaining case 80 make a half turn, at the same time, the reagent table 37 makes a half turn, and the stirring table 56 also makes a half turn through the swing connection means 86. Therefore, the positions of the pair of reagent holders 38, 38 attached to the reagent table 37 are interchanged. When the particle solution in the particle bin 40 of one reagent holder 38 and the labeling reagent in the labeling reagent bin 42 are used up, it is possible to immediately switch to a new reagent holder 38.

In the automatic analyzer 12 of the present invention, the configuration of the reagent holder 38 detachably mounted on the reagent table 37 is not known in the prior art.
This will be described in more detail with reference to FIG.

The reagent holder 38 is composed of a pair of holder pieces 96 and 98 which are openably and closably engaged via a hinge 94 provided at one end. The particle bin 40 is attached to each holder piece 96, 98.
And a locking hole 100 for locking the upper part of the labeling reagent bottle 42,
Semicircular portions 100a, 100b and 102 forming
a, 102b are provided.

An engaging projection 104 projects from the lower end of one holder piece 96, and an engaging receiving section 106 is provided at the lower end of the other holder piece 98. For example, the holder pieces 96 and 98 are opened and the semicircular portion 1
The holder bins 96 and 98 are closed by positioning the particle bin 40 and the labeling reagent bin 42 so as to correspond to 00a, 100b and 102a, 102b (FIG. 6). The particle bin 40 and the labeling reagent bin 42
The upper portion is locked at 0 and 102, and the lower portion is held in a rotatable state (FIG. 5).

The particle bin 40 and the labeling reagent bin 4
2 is removed, the engagement between the engagement projection 104 and the engagement receiving portion 106 is released, and the holder pieces 96, 98 are removed.
Can be opened.

With the particle bin 40 locked on the reagent holder 38 at its upper part, as described above,
7 (a) and 7 (b) when the lower part is horizontally rotated.
As shown in (c), the particle bin 40 rotates while always tilting. Therefore, since the tip of the nozzle 26a of the particle supply means 26 can be positioned at the inclined bottom, there is an advantage that the particle solution remaining as an unsuckable component can be minimized.

As is clear from FIGS. 7 (a), 7 (b) and 7 (d), since the labeling reagent does not need to be stirred, the operation is performed without rotating and maintaining the stationary state. However, it is needless to say that the horizontal rotation movement can be performed by applying the same configuration as the mechanism for horizontally rotating the particle bin 40 as necessary.

In FIG. 5, reference numeral 108 denotes the reagent holder 3.
A bar code provided on the front surface of one holder piece 96 of 8 indicates measurement items, operating conditions, and the like. This barcode 1
08 can be automatically read by the barcode reader 110 provided in the reagent table mechanism 36 to automatically advance the analysis operation.

The operation of the above configuration will be described.
When a switch means (not shown) is turned on, the motor of the automatic immunoassay device 12 is turned on and started, and the reaction tube turret 14 intermittently rotates at a predetermined speed, and the reaction tube holder 16 and the reaction tube holder 16 are rotated. The held reaction tube also rotates.

The stirring motor 46 operates according to a command from a computer in accordance with a preset program. When the stirring motor 46 starts, the stirring shaft 52 rotates via the first drive pulley 50, the timing belt 62, and the first pulley 60, and the eccentric pin 54 at the upper end of the stirring shaft 52 rotates eccentrically.

This eccentric rotation is transmitted to the stirring table 56 via the bearing 58, and the stirring table 56 performs horizontal rotation. Since the lower part of the particle bin 40 is inserted into the insertion hole 57 formed in the stirring table 56, the lower part of the particle bin 40 is also rotated in accordance with the horizontal movement of the stirring table 56. The particle solution stored in the particle bin 40 is constantly stirred, and the particles in the solution maintain a uniformly dispersed floating state and do not settle.

A play insertion hole 5 formed in the stirring table 56
Although the labeling reagent bin 42 is loosely inserted in the nozzle 9, the diameter of the loose insertion hole 59 is larger than the horizontal movement range of the stirring table 56. The stationary state is maintained without moving.

In this state, a predetermined amount of the particle solution in which the particles are uniformly suspended is supplied from the particle bin 40 to the reaction tube by the particle supply means 26, and the labeling reagent dispensing means 28 supplies the label from the labeling reagent bin 42 to the reaction tube. Dispense a predetermined amount of reagent into a reaction tube,
A predetermined reaction is performed.

Since the lower part of the particle bin 40 is rotated, as shown in FIG. 7C, the nozzle 26a of the particle supply means 26 is inserted inside the particle bin 40 in an inclined state. Inserted,
The particle solution is sucked. If the particle bin 40 is inclined as described above, the particle solution can be sucked from the peripheral portion of the inner bottom portion of the particle bin 40, so that there is an advantage that the unsuckable amount of the particle solution can be minimized.

Since the particle solution is expensive and the solution that cannot be sucked is the only option to be discarded, minimizing the unsuctionable amount is cost-effective. Since the labeling reagent is prepared as a homogeneous solution from the beginning, there is no need to stir, and since it is relatively inexpensive, the necessity to tilt and hold it to minimize the volume that cannot be aspirated is not so large at present. .

However, if the diameter of the loose insertion hole 59 formed in the stirring table 56 is made small and the insertion hole is used for the labeling reagent bin 42, the labeling reagent can be stirred, and the amount of suction that cannot be suctioned can be increased. It is needless to say that the suction can be performed with the minimum amount.

When all of the particle solution in the particle bin 40 and the labeling reagent in the labeling reagent bin 42 are sucked, another particle bin 40 containing a new particle solution and another labeling reagent containing a new labeling reagent are used. It needs to be replaced with a bin 42. If the reagent table 37 has only one reagent holder receiving hole 37a, the used reagent holder 38 is removed from the reagent holder receiving hole 37a and a new reagent holder 38 is inserted. However, if two reagent holder receiving holes 37a are provided in the reagent table 37 and the reagent holders 38 are inserted into the respective holes as in the above-described embodiment, the used reagent holder is rotated by rotating the reagent table 37. 38 and new reagent holder 3
8 can be exchanged.

As described above, according to the automatic analyzer of the present invention, by rotating the particle bin, the particle solution is stirred so that the particles are not uniformly diffused and settled, so that the conventional stirring rod is used. This eliminates the need for agitation processing of the particle solution and eliminates the possibility of mixing other types of solutions (contamination), thereby simplifying the analysis operation. In addition, since the unsuckable amount of the particle solution can be minimized, the amount of waste of the expensive particle solution can be minimized and the cost can be reduced.

[Brief description of the drawings]

FIG. 1 is a top view showing one embodiment of an automatic immunoassay apparatus according to the present invention.

FIG. 2 is a front view of FIG.

FIG. 3 is a top view of a main part of the automatic immunoassay apparatus of the present invention.

FIG. 4 is a sectional view of FIG. 3;

FIG. 5 is an enlarged perspective view of a reagent holder that locks a particle bin and a labeled reagent bin.

6 is an enlarged perspective view showing a state in which the locking of the particle bin and the labeling reagent bottle is released from the state of FIG. 5;

FIGS. 7A and 7B are diagrams showing a horizontal rotation state of the particle bin, wherein FIG. 7A is a top view, FIG.
(C) is a sectional view taken along line CC, and (d) is a sectional view taken along line DD.

FIG. 8 is an explanatory sectional view showing a connection state between a stirring table and a rotary plate.

[Explanation of symbols]

12 automatic analyzer, 14 reaction tube turret, 16
Reaction tube holder, 18 reaction tube holding portion, 18a inner periphery, 18b outer periphery, 20 reaction tube rack, 21 receiving portion, 22 reaction tube transfer means, 22a reaction tube gripper, 2
6 particle supply means, 26a particle nozzle, 28 reagent dispensing means, 28a reagent nozzle, 30
Washing means, 32 reagent dispensing means for measurement, 34 measuring means, 36 reagent table mechanism, 37 reagent table, 3
7a mounting hole, 38 reagent holder, 40 particle bottle, 42 labeled reagent bottle, 44 substrate, 46 stirring motor, 48 support, 50 first drive pulley, 52 stirring shaft, 54 eccentric pin, 56 stirring table, 5
7 Insertion hole, 58 Bearing, 59 Free insertion hole, 60
1st pulley, 62 first timing belt, 64 fixed sleeve, 66 support plate, 68 support leg, 70 table rotation motor, 72 second drive pulley, 74 rotation pulley, 76 second timing belt, 77 support, 78
Rotating plate, 80 insulation case, 82 insulation material, 84
Connecting part, 86 swing connecting means, 88 connecting rod, 9
0,92 spherical joint, 94 hinge part, 96, 98 holder piece, 100, 102 locking hole, 100a, 100
b, 102a, 102b semicircular portion, 104 engaging projection,
106 engagement receiving portion, 108 barcode, 110 barcode reader

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[Procedure amendment]

[Submission date] July 16, 1998

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0004

[Correction method] Change

[Correction contents]

A method of measuring the amount of antigen contained in a sample such as blood collected from a patient as a sample by a one-step non-competitive sandwich method will be described. For example, an antibody bound to an insoluble substance (solid phase) such as an inner wall or a particle (particle) of a synthetic resin container (hereinafter referred to as “solid phase antibody”)
If, radioactive substances, fluorescent substances, antibody conjugated with a labeling substance enzyme arsenide (hereinafter referred to as "labeled antibody") and the reaction tube are added in advance, adding an analyte to be measured.

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0007

[Correction method] Change

[Correction contents]

On the other hand, in the two-step non-competitive sandwich method, the first reaction is carried out by adding a sample to a reaction tube to which only a solid phase antibody has been previously added, and then a labeled antibody is added. This is a method in which a second reaction is performed by adding a substance to perform measurement.

Claims (10)

[Claims] 1. An automatic analyzer for rotating a reaction tube turret supporting a large number of reaction tubes and sequentially supplying particles and dispensing a labeling reagent to each reaction tube to perform an automatic analysis, An automatic analyzer, wherein a particle bin for storing a particle solution containing particles is rotated, and the particle solution is stirred so that the particles are uniformly dispersed. 2. A reagent holding means for holding an upper portion of the particle bin and holding the lower portion in a rotatable state, wherein the lower portion of the particle bin is rotated around an upper locking portion of the particle bin as a fulcrum. 2. The automatic analyzer according to claim 1, wherein the automatic analyzer is operated. 3. A particle remaining as an unsuckable component in the particle bin by rotating the particle bin in a state of being always inclined so as to be able to suck a particle solution located on the inner peripheral edge of the particle bin. 3. The automatic analyzer according to claim 1, wherein the amount of the solution can be minimized. 4. A reagent holding means, a stirring table provided below the reagent holding means and having a particle hole inserted into an insertion hole formed in a peripheral portion thereof, and an eccentric pin projected on an upper end portion. A stirring shaft formed by connecting the eccentric pin to a central lower surface of the stirring table via a bearing, and first driving means for rotating the stirring shaft. 4. The particle table according to claim 2, wherein the pin moves eccentrically and the stirring table horizontally rotates, whereby the lower part of the particle bin is rotated about the upper locking part of the particle bin as a fulcrum. Automatic analyzer. 5. A rotating plate provided below the stirring table, swing connection means for connecting the stirring table and the rotating plate so as to be able to rotate horizontally with each other, and rotatably attached to the stirring shaft. The apparatus further includes a rotating pulley connected to the rotating plate, and second driving means for rotating the rotating pulley, wherein a plurality of particle bins are held by the reagent holding means, and the rotating pulley is rotated. 5. The automatic analyzer according to claim 4, wherein the plurality of particle bins are sequentially positioned at a particle supply position. 6. A reagent holder comprising: a reagent holder in which an upper part of a particle bin is detachably locked and a lower part is rotatable; and a reagent table which detachably holds the reagent holder. An automatic analyzer according to any one of claims 2 to 5. 7. The reagent holder removably locks the upper portions of the particle bin and the labeling reagent bin, and the label holder has a play hole for inserting the particle reagent bin in the stirring table in addition to the insertion hole for inserting the particle bin. An insertion hole is formed, and the lower part of the labeling reagent bottle is loosely inserted into the play insertion hole. When the stirring table rotates horizontally, the particle bin rotates with the movement of the stirring table. 7. The automatic analyzer according to claim 6, wherein the bottle does not come into contact with the stirring table during the movement of the stirring table and maintains a stationary state. 8. The swing connection means has a connection rod, and an upper part of the connection rod is pivotally mounted on a lower surface of the stirring table and a lower part of the connection rod is pivotally mounted on an upper surface of the rotary plate. The automatic analyzer according to any one of claims 5 to 7, wherein the stirring table and the rotating plate are connected to each other so as to be horizontally rotatable, and the rotating plate and the reagent table are connected to each other. . 9. The heat insulation case for keeping the particle bin and the labeling reagent bottle warm is provided below the reagent table so as to surround the rotary plate from below and outside. The automatic analyzer as described. 10. The automatic analyzer according to claim 7, wherein a barcode indicating the components of the particle solution and the labeling reagent is attached to a front surface of the reagent holder.