JPH11512620A - 発現されたタンパク質の検出および精製のためのコイルドコイルヘテロダイマー法および組成物 - Google Patents

発現されたタンパク質の検出および精製のためのコイルドコイルヘテロダイマー法および組成物

Info

Publication number
JPH11512620A
JPH11512620A JP9514507A JP51450797A JPH11512620A JP H11512620 A JPH11512620 A JP H11512620A JP 9514507 A JP9514507 A JP 9514507A JP 51450797 A JP51450797 A JP 51450797A JP H11512620 A JPH11512620 A JP H11512620A
Authority
JP
Japan
Prior art keywords
heterodimer
peptide
subunit
coil
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP9514507A
Other languages
English (en)
Japanese (ja)
Inventor
イー．，ジュニア ヒューストン，マイケル
ユ，レイ
バウティスタ，デイジー
エス． ホッジズ，ロバート
トリペット，ブライアン
ジェイ． カチア，ポール
ティー． アービン，ランドール
Original Assignee
ペンス
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ペンス filed Critical ペンス
Publication of JPH11512620A publication Critical patent/JPH11512620A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Gram-negative bacteria
    • C07K16/1214—Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP9514507A 1995-10-06 1996-10-04 発現されたタンパク質の検出および精製のためのコイルドコイルヘテロダイマー法および組成物 Pending JPH11512620A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US54039795A 1995-10-06 1995-10-06
US08/540,397 1995-10-06
PCT/US1996/016032 WO1997012988A1 (en) 1995-10-06 1996-10-04 Coiled-coil heterodimer methods and compositions for the detection and purification of expressed proteins

Publications (1)

Publication Number Publication Date
JPH11512620A true JPH11512620A (ja) 1999-11-02

Family

ID=24155285

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9514507A Pending JPH11512620A (ja) 1995-10-06 1996-10-04 発現されたタンパク質の検出および精製のためのコイルドコイルヘテロダイマー法および組成物

Country Status (4)

Country Link
EP (1) EP0854931A4 (de)
JP (1) JPH11512620A (de)
AU (1) AU695679B2 (de)
WO (1) WO1997012988A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2022505866A (ja) * 2018-11-02 2022-01-14 ユニバーシティ オブ ワシントン 直交タンパク質ヘテロ二量体

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6271198B1 (en) 1996-11-06 2001-08-07 Genentech, Inc. Constrained helical peptides and methods of making same
GB9714680D0 (en) 1997-07-11 1997-09-17 Medical Res Council Recombinant production of proteins
GB9714681D0 (en) * 1997-07-11 1997-09-17 Medical Res Council Recombinant production of proteins
GB9718358D0 (en) * 1997-08-30 1997-11-05 Univ Leeds Chemical modification
US7105307B2 (en) 1997-08-30 2006-09-12 Cyclacel, Ltd. Compositions and methods for screening for modulators of enzymatic activity
DE19815331A1 (de) * 1998-04-06 1999-10-14 Karlsruhe Forschzent Transkriptionsfaktoren und deren Verwendung
CA2329689A1 (en) 1998-06-12 1999-12-23 The Governors Of The University Of Alberta Composition and method for treatment of pseudomonas
US6380365B1 (en) * 1998-09-04 2002-04-30 Affitech As Temperature dependent ligand facilitated purification of proteins
GB2341115B (en) * 1998-09-04 2002-02-06 Actinova Ltd Chromatography
WO2002067969A2 (en) * 2001-02-21 2002-09-06 Medtronic Minimed, Inc. Stabilized insulin formulations
GB0315993D0 (en) * 2003-07-08 2003-08-13 Sussex Ip Ltd Peptide-based linker
WO2005024035A1 (en) * 2003-09-05 2005-03-17 National Research Council Of Canada Coiled-coil fusion proteins comprising cell receptor domains
US7786261B2 (en) 2004-09-02 2010-08-31 National Research Council Of Canada Coiled-coil fusion proteins comprising cell receptor domains
CN102939101A (zh) 2010-01-26 2013-02-20 科罗拉多大学董事会，法人 用于通用疫苗的流感病毒组合物和方法
AU2011286024B2 (en) * 2010-08-02 2014-08-07 Macrogenics, Inc. Covalent diabodies and uses thereof
US12281161B2 (en) 2021-07-28 2025-04-22 Trustees Of Boston University Polypeptides and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4040669A1 (de) * 1990-12-19 1992-06-25 Behringwerke Ag Verwendung von peptidpaaren mit extrem hoher spezifischer affinitaet zueinander im bereich der in vitro diagnostik
ATE196297T1 (de) * 1994-05-18 2000-09-15 Synthetic Peptides Inc Heterodimere trägerzusammensetzung von immunogenen polypeptiden und verfahren zu deren verwendung

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2022505866A (ja) * 2018-11-02 2022-01-14 ユニバーシティ オブ ワシントン 直交タンパク質ヘテロ二量体
JP2024149492A (ja) * 2018-11-02 2024-10-18 ユニバーシティ オブ ワシントン 直交タンパク質ヘテロ二量体

Also Published As

Publication number Publication date
EP0854931A4 (de) 2002-02-13
AU695679B2 (en) 1998-08-20
AU7258496A (en) 1997-04-28
WO1997012988A1 (en) 1997-04-10
EP0854931A1 (de) 1998-07-29

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