JPS54119085A - Alkaliprotease - Google Patents

Alkaliprotease

Info

Publication number
JPS54119085A
JPS54119085A JP2730178A JP2730178A JPS54119085A JP S54119085 A JPS54119085 A JP S54119085A JP 2730178 A JP2730178 A JP 2730178A JP 2730178 A JP2730178 A JP 2730178A JP S54119085 A JPS54119085 A JP S54119085A
Authority
JP
Japan
Prior art keywords
activity
lysine
stable
carboxyl group
heating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2730178A
Other languages
Japanese (ja)
Other versions
JPS6222600B2 (en
Inventor
Takeji Masaki
Masami Soejima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda Pharmaceutical Co Ltd
Original Assignee
Takeda Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd
Priority to JP2730178A priority Critical patent/JPS54119085A/en
Publication of JPS54119085A publication Critical patent/JPS54119085A/en
Publication of JPS6222600B2 publication Critical patent/JPS6222600B2/ja
Granted legal-status Critical Current

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

NEW MATERIAL:The title substance characterized by: molecular weight 27,000 ± 1,000; peak of UV-abasorption specturm, 275W280 mμ, IR absorption spectrum, characteristic absorptions of protein; isoelectric point, about pH7; soluble in water and aqueous solution of NaCl having an ion strength of 0.01W0.1; optimum pH range for protease activity, ≥8,5; that for esterase activity, ≥7.5; that for amidase activity, about 8.5; optimum temperature for protease activity, about 50°C; stable at pH5W12 at a low temperature; stable by heating at 40°C and pH9.5 for 60 min; more than 90% of the activity is lost by heating at 70°C for 60 min; selectively and specifically hydrolyzes the ester bond and amide bond of carboxyl group in L-lysine; inhibited by diisopropyl phosphofluoride, etc.
USE: Selective and specific hydrolysis of ester bond and amide bond of carboxyl group in L-lysine.
PROCESS: Culturing of Achromobacter (e.g. Achromobacter lyticus M497-1).
COPYRIGHT: (C)1979,JPO&Japio
JP2730178A 1978-03-09 1978-03-09 Alkaliprotease Granted JPS54119085A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2730178A JPS54119085A (en) 1978-03-09 1978-03-09 Alkaliprotease

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2730178A JPS54119085A (en) 1978-03-09 1978-03-09 Alkaliprotease

Publications (2)

Publication Number Publication Date
JPS54119085A true JPS54119085A (en) 1979-09-14
JPS6222600B2 JPS6222600B2 (en) 1987-05-19

Family

ID=12217259

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2730178A Granted JPS54119085A (en) 1978-03-09 1978-03-09 Alkaliprotease

Country Status (1)

Country Link
JP (1) JPS54119085A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5951789A (en) * 1982-09-18 1984-03-26 Wako Pure Chem Ind Ltd Stabilized enzyme

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5951789A (en) * 1982-09-18 1984-03-26 Wako Pure Chem Ind Ltd Stabilized enzyme

Also Published As

Publication number Publication date
JPS6222600B2 (en) 1987-05-19

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