JPS55162060A - Measuring method for glycosyl hemoglobin - Google Patents

Measuring method for glycosyl hemoglobin

Info

Publication number
JPS55162060A
JPS55162060A JP6977379A JP6977379A JPS55162060A JP S55162060 A JPS55162060 A JP S55162060A JP 6977379 A JP6977379 A JP 6977379A JP 6977379 A JP6977379 A JP 6977379A JP S55162060 A JPS55162060 A JP S55162060A
Authority
JP
Japan
Prior art keywords
hemoglobin
developer
sodium azide
glycosyl
methemoglobin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP6977379A
Other languages
Japanese (ja)
Inventor
Toshiaki Tsutsui
Hideki Jinno
Kozo Muramatsu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Chemical Corp
Original Assignee
Mitsubishi Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Chemical Industries Ltd filed Critical Mitsubishi Chemical Industries Ltd
Priority to JP6977379A priority Critical patent/JPS55162060A/en
Publication of JPS55162060A publication Critical patent/JPS55162060A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PURPOSE: To secure the simple and high-reproducibility measurement for the glycosyl hemoglobin in the blood, by giving the analysis to the pre-processed sample through the chromatography method using the specified developer.
CONSTITUTION: The sodium azide of about 40W1,000ml/l is added to the blood in which the erythrocyte is subjected to hemolysis, and thus the contained glycosyl hemoglobin and the hemoglobin are modified to methemoglobin each to obtain azido methoglycosyl hemoglobin (a) and azido methemoglobin (b) respectively which are used for the sample of the liquid chromatography. In other words, the sodium azide aqueous solution (preferably pH6.6W7.1) of about 400W1,000ml/l is used for the 1st developer of the chromatogrphy in order to elute component (a). Then the 2nd developer (such as the phosphoric acid buffer solution) which contains no sodium azide is used to elute component (b). Then the components isolated and eluted are measured by the spectro-photometer.
COPYRIGHT: (C)1980,JPO&Japio
JP6977379A 1979-06-04 1979-06-04 Measuring method for glycosyl hemoglobin Pending JPS55162060A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP6977379A JPS55162060A (en) 1979-06-04 1979-06-04 Measuring method for glycosyl hemoglobin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6977379A JPS55162060A (en) 1979-06-04 1979-06-04 Measuring method for glycosyl hemoglobin

Publications (1)

Publication Number Publication Date
JPS55162060A true JPS55162060A (en) 1980-12-17

Family

ID=13412433

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6977379A Pending JPS55162060A (en) 1979-06-04 1979-06-04 Measuring method for glycosyl hemoglobin

Country Status (1)

Country Link
JP (1) JPS55162060A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5872055A (en) * 1981-10-26 1983-04-28 Sogo Seibutsu Igaku Kenkyusho:Kk Simple measuring method for hemoglobin a1 in blood
CN102893147A (en) * 2010-03-31 2013-01-23 积水医疗株式会社 Method of analyzing hemoglobins

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5872055A (en) * 1981-10-26 1983-04-28 Sogo Seibutsu Igaku Kenkyusho:Kk Simple measuring method for hemoglobin a1 in blood
CN102893147A (en) * 2010-03-31 2013-01-23 积水医疗株式会社 Method of analyzing hemoglobins
US9304139B2 (en) 2010-03-31 2016-04-05 Sekisui Medical Co., Ltd. Method of analyzing hemoglobins

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