JPS5520721A - Separation of biologically active material from hematopiesis tissue of domestic animal - Google Patents
Separation of biologically active material from hematopiesis tissue of domestic animalInfo
- Publication number
- JPS5520721A JPS5520721A JP9350978A JP9350978A JPS5520721A JP S5520721 A JPS5520721 A JP S5520721A JP 9350978 A JP9350978 A JP 9350978A JP 9350978 A JP9350978 A JP 9350978A JP S5520721 A JPS5520721 A JP S5520721A
- Authority
- JP
- Japan
- Prior art keywords
- solution
- biologically active
- domestic animal
- active material
- spleen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000011149 active material Substances 0.000 title abstract 3
- 238000000926 separation method Methods 0.000 title 1
- 239000000243 solution Substances 0.000 abstract 5
- 239000000463 material Substances 0.000 abstract 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract 2
- 210000000952 spleen Anatomy 0.000 abstract 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 abstract 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 229920005654 Sephadex Polymers 0.000 abstract 1
- 239000012507 Sephadex™ Substances 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 238000004108 freeze drying Methods 0.000 abstract 1
- 239000007789 gas Substances 0.000 abstract 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 239000003456 ion exchange resin Substances 0.000 abstract 1
- 229920003303 ion-exchange polymer Polymers 0.000 abstract 1
- 229910052742 iron Inorganic materials 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 210000004165 myocardium Anatomy 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 239000000843 powder Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000011347 resin Substances 0.000 abstract 1
- 229920005989 resin Polymers 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 230000004936 stimulating effect Effects 0.000 abstract 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
PURPOSE: To separate a biologically active material for use as a stimulating material in the segeneration of heart muscle and a growth controlling material, by purifying a manow or spleen of domestic animal, followed by treatment.
CONSTITUTION: A narrow or spleen of a domestic animal is dipped into a buffer solution in the pH of 3.4W7.5 or in 30W99.5 wt.% alcohol solution to prepare a dipped solution, which is dialyzed for a night. Hydrogen sulfide gas is introduced into the dialyzed solution so that iron is precipitated and removed. Colored impurities are removed by adding 0.01W0.5 wt.% based on a liquid amount of active carbon to the solution or by using a decoloring resin. The solution thus obtained is directly subjected to freeze drying or after being passed through Sephadex or an ion exchange resin to separate biologically active material. This material is yellow solid powder, has 14 wt.% of nitrogen, and consists mainly of a protein exhibiting a maximum absorption in a wave length of 270W280 mm.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9350978A JPS5520721A (en) | 1978-07-31 | 1978-07-31 | Separation of biologically active material from hematopiesis tissue of domestic animal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9350978A JPS5520721A (en) | 1978-07-31 | 1978-07-31 | Separation of biologically active material from hematopiesis tissue of domestic animal |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5520721A true JPS5520721A (en) | 1980-02-14 |
Family
ID=14084308
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9350978A Pending JPS5520721A (en) | 1978-07-31 | 1978-07-31 | Separation of biologically active material from hematopiesis tissue of domestic animal |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5520721A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5534615A (en) * | 1994-04-25 | 1996-07-09 | Genentech, Inc. | Cardiac hypertrophy factor and uses therefor |
| US5624806A (en) * | 1994-04-25 | 1997-04-29 | Genentech, Inc. | Antibodies to cardiac hypertrophy factor and uses thereof |
| US6472585B1 (en) | 1994-04-25 | 2002-10-29 | Genentech, Inc. | Cardiotrophin-1 defective mouse |
| US7258983B2 (en) | 1994-04-25 | 2007-08-21 | Genentech, Inc. | Cardiotrophin-1 compositions and methods for the treatment of tumor |
-
1978
- 1978-07-31 JP JP9350978A patent/JPS5520721A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5534615A (en) * | 1994-04-25 | 1996-07-09 | Genentech, Inc. | Cardiac hypertrophy factor and uses therefor |
| US5624806A (en) * | 1994-04-25 | 1997-04-29 | Genentech, Inc. | Antibodies to cardiac hypertrophy factor and uses thereof |
| US5627073A (en) * | 1994-04-25 | 1997-05-06 | Genentech, Inc. | Hybridomas producing antibodies to cardiac hypertrophy factor |
| US5679545A (en) * | 1994-04-25 | 1997-10-21 | Genentech, Inc. | Gene encoding cardiac hypertrophy factor |
| US5723585A (en) * | 1994-04-25 | 1998-03-03 | Genentech, Inc. | Method of purifying cardiac hypertrophy factor |
| US6117650A (en) * | 1994-04-25 | 2000-09-12 | Genentech, Inc. | Assay for cardiac hypertrophy |
| US6472585B1 (en) | 1994-04-25 | 2002-10-29 | Genentech, Inc. | Cardiotrophin-1 defective mouse |
| US7258983B2 (en) | 1994-04-25 | 2007-08-21 | Genentech, Inc. | Cardiotrophin-1 compositions and methods for the treatment of tumor |
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