JPS5530655A - Latex sensitive to human igg antibody for quantitizing human igg and its particle composite - Google Patents

Latex sensitive to human igg antibody for quantitizing human igg and its particle composite

Info

Publication number
JPS5530655A
JPS5530655A JP10389378A JP10389378A JPS5530655A JP S5530655 A JPS5530655 A JP S5530655A JP 10389378 A JP10389378 A JP 10389378A JP 10389378 A JP10389378 A JP 10389378A JP S5530655 A JPS5530655 A JP S5530655A
Authority
JP
Japan
Prior art keywords
human igg
latex
igg
buffer liquid
quantitizing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP10389378A
Other languages
Japanese (ja)
Inventor
Tetsuo Tomiyama
Taku Ogura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Priority to JP10389378A priority Critical patent/JPS5530655A/en
Priority to CH776779A priority patent/CH645726A5/en
Priority to GB7929796A priority patent/GB2030293B/en
Priority to DE19792934757 priority patent/DE2934757C2/en
Priority to FR7921529A priority patent/FR2435040A1/en
Publication of JPS5530655A publication Critical patent/JPS5530655A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE: To make it possible to simply and highly sensitively quantitize the human IgG without being required to condense urine or the like by sensitizing special latex particles to human immunity globulinG(IgG) and by making the same contains special stabilizing agent.
CONSTITUTION: Human IgG is injected into rabbit or the like, and blood is sampled to obtain antiserum, from which a purified human IgG. The purified human IgG diluted with the mixture of a phosphoric salt buffer liquid and the physiological solution of sodium chloride is reacted with in a buffer liquid of pH 6.5 to 8 or polystyrene latex, which has a narrow diameter distribution of 0.01 to 10 (or 0.1 to 1.0)μ ranging from 0.2 to 0.005μ and having specific weight of 0.9 to 1.4 while having a latex particle concentration of 0.05 to 5%. After this reaction, the centrifugal separation and rinsing are performed. Finally, the latex is suspended in a dilution which is prepared by adding arbumin of cow serum and NaN3 to the aqueous solution of for buffering the phophoric acid or glycine with a view to preventing coaggulation.
COPYRIGHT: (C)1980,JPO&Japio
JP10389378A 1978-08-28 1978-08-28 Latex sensitive to human igg antibody for quantitizing human igg and its particle composite Pending JPS5530655A (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP10389378A JPS5530655A (en) 1978-08-28 1978-08-28 Latex sensitive to human igg antibody for quantitizing human igg and its particle composite
CH776779A CH645726A5 (en) 1978-08-28 1979-08-27 PREPARATION FOR DETERMINING HUMAN IMMUNOGLOBULIN G, METHOD FOR THE PRODUCTION THEREOF AND ITS USE.
GB7929796A GB2030293B (en) 1978-08-28 1979-08-28 Composition for determination of human immunoglobulin g process for its preparation and its use
DE19792934757 DE2934757C2 (en) 1978-08-28 1979-08-28 Composition and method for the determination of human immunoglobulin-G
FR7921529A FR2435040A1 (en) 1978-08-28 1979-08-28 COMPOSITION FOR DETERMINING HUMAN IMMUNOGLOBULIN G, METHOD FOR ITS PREPARATION AND ITS USE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10389378A JPS5530655A (en) 1978-08-28 1978-08-28 Latex sensitive to human igg antibody for quantitizing human igg and its particle composite

Publications (1)

Publication Number Publication Date
JPS5530655A true JPS5530655A (en) 1980-03-04

Family

ID=14366092

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10389378A Pending JPS5530655A (en) 1978-08-28 1978-08-28 Latex sensitive to human igg antibody for quantitizing human igg and its particle composite

Country Status (5)

Country Link
JP (1) JPS5530655A (en)
CH (1) CH645726A5 (en)
DE (1) DE2934757C2 (en)
FR (1) FR2435040A1 (en)
GB (1) GB2030293B (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56140255A (en) * 1980-04-03 1981-11-02 Terumo Corp Detecting reagent of antibody for antiacetylcholine receptor, and its using method
JPS59193356A (en) * 1983-03-17 1984-11-01 バイオウィッテッカー・インコーポレーテッド Fluorophotometric analysis of total IgE concentration and reagents therefor
CN105929171A (en) * 2016-04-13 2016-09-07 柏荣诊断产品（上海）有限公司 High performance human urine immune globulin G detection kit
JP2021170021A (en) * 2016-10-03 2021-10-28 ヒュービットジェノミクス株式会社 Testing method that enables specific diagnosis of early pathology of diabetic nephropathy

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4331649A (en) 1978-10-10 1982-05-25 Burroughs Wellcome Co. Immune complex assay
FI792576A7 (en) * 1979-08-20 1981-01-01 Orion Yhtymae Oy Improved enzyme immunoassay method.
CA1206876A (en) * 1982-09-13 1986-07-02 Ernest C. Adams Method for measuring igg in a neonatal foal or calf and in colostrum
JPS59100863A (en) * 1982-12-01 1984-06-11 Fuji Seiyaku Kogyo Kk Test slide for diagnosis and its production
DE3303083A1 (en) * 1983-01-31 1984-08-02 Behringwerke Ag, 3550 Marburg IMMUNOLOGICAL LATEX AGGLUTINATION METHOD AND AGENT
EP0194156B1 (en) * 1985-03-08 1990-02-21 Sanko Junyaku Co., Ltd. Method of measuring the amount of immune antibody in serum

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3088875A (en) * 1959-10-27 1963-05-07 Hyland Lab Immunological diagnostics utilizing polystyrene latex particles of 0.15 to 0.25 micron
NL125235C (en) * 1965-04-15
GB1384399A (en) * 1971-02-01 1975-02-19 Hoffmann La Roche Automated method of obtaining serological data

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56140255A (en) * 1980-04-03 1981-11-02 Terumo Corp Detecting reagent of antibody for antiacetylcholine receptor, and its using method
JPS59193356A (en) * 1983-03-17 1984-11-01 バイオウィッテッカー・インコーポレーテッド Fluorophotometric analysis of total IgE concentration and reagents therefor
CN105929171A (en) * 2016-04-13 2016-09-07 柏荣诊断产品（上海）有限公司 High performance human urine immune globulin G detection kit
JP2021170021A (en) * 2016-10-03 2021-10-28 ヒュービットジェノミクス株式会社 Testing method that enables specific diagnosis of early pathology of diabetic nephropathy

Also Published As

Publication number Publication date
CH645726A5 (en) 1984-10-15
FR2435040B1 (en) 1984-09-21
GB2030293A (en) 1980-04-02
DE2934757A1 (en) 1980-03-13
FR2435040A1 (en) 1980-03-28
GB2030293B (en) 1983-03-23
DE2934757C2 (en) 1984-12-20

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