JPS554308A - Isolation of deoxyribonucleic acid from milt of fish - Google Patents
Isolation of deoxyribonucleic acid from milt of fishInfo
- Publication number
- JPS554308A JPS554308A JP7540078A JP7540078A JPS554308A JP S554308 A JPS554308 A JP S554308A JP 7540078 A JP7540078 A JP 7540078A JP 7540078 A JP7540078 A JP 7540078A JP S554308 A JPS554308 A JP S554308A
- Authority
- JP
- Japan
- Prior art keywords
- milt
- fish
- homogenized
- deoxyribonucleic acid
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000251468 Actinopterygii Species 0.000 title abstract 4
- 102000053602 DNA Human genes 0.000 title abstract 4
- 108020004414 DNA Proteins 0.000 title abstract 4
- 238000002955 isolation Methods 0.000 title 1
- 239000003513 alkali Substances 0.000 abstract 2
- 230000009615 deamination Effects 0.000 abstract 2
- 238000006481 deamination reaction Methods 0.000 abstract 2
- 230000006866 deterioration Effects 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 238000013019 agitation Methods 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000010790 dilution Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 150000007522 mineralic acids Chemical class 0.000 abstract 1
- 150000007524 organic acids Chemical class 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Saccharide Compounds (AREA)
Abstract
PURPOSE: Homogenized milt of fish is made to a specific acidic value in pH to permit agitation without dilution with water and prevent the deterioration caused by deamination or others and followed by extraction with alkali, thus isolating DNA, an intermediate for drugs or food.
CONSTITUTION: After homogenized, milt of fish is adjusted to 4.5W6.5 in pH by inorganic or organic acid and stirred at 80W100°C for 0.5W2 hr. Then, an alkali aqueous solution is added to adjust the pH to over 8 and the stirring is continued, the solid part is separated and an acid is added to the filtrate to isolate deoxyribonucleic acid (DNA). The adjustment of the homogenized milt of fish to a specific pH value permits the increase in quantity to be treated per batch and the deterioration caused by deamination or others on heating can be prevented.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7540078A JPS554308A (en) | 1978-06-23 | 1978-06-23 | Isolation of deoxyribonucleic acid from milt of fish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7540078A JPS554308A (en) | 1978-06-23 | 1978-06-23 | Isolation of deoxyribonucleic acid from milt of fish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS554308A true JPS554308A (en) | 1980-01-12 |
Family
ID=13575081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7540078A Pending JPS554308A (en) | 1978-06-23 | 1978-06-23 | Isolation of deoxyribonucleic acid from milt of fish |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS554308A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4985552A (en) * | 1986-04-17 | 1991-01-15 | Crinos Industria Farmacobiologica S.P.A. | Process for obtaining chemically defined and reproducible polydeoxyribonucleotides |
-
1978
- 1978-06-23 JP JP7540078A patent/JPS554308A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4985552A (en) * | 1986-04-17 | 1991-01-15 | Crinos Industria Farmacobiologica S.P.A. | Process for obtaining chemically defined and reproducible polydeoxyribonucleotides |
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