JPS554308A - Isolation of deoxyribonucleic acid from milt of fish - Google Patents

Isolation of deoxyribonucleic acid from milt of fish

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Publication number
JPS554308A
JPS554308A JP7540078A JP7540078A JPS554308A JP S554308 A JPS554308 A JP S554308A JP 7540078 A JP7540078 A JP 7540078A JP 7540078 A JP7540078 A JP 7540078A JP S554308 A JPS554308 A JP S554308A
Authority
JP
Japan
Prior art keywords
milt
fish
homogenized
deoxyribonucleic acid
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP7540078A
Other languages
Japanese (ja)
Inventor
Nobutaka Sugimoto
Kunio Yokozawa
Hifumi Kishimoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
YUKI GOSEI YAKUHIN KOGYO KK
Original Assignee
YUKI GOSEI YAKUHIN KOGYO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by YUKI GOSEI YAKUHIN KOGYO KK filed Critical YUKI GOSEI YAKUHIN KOGYO KK
Priority to JP7540078A priority Critical patent/JPS554308A/en
Publication of JPS554308A publication Critical patent/JPS554308A/en
Pending legal-status Critical Current

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Abstract

PURPOSE: Homogenized milt of fish is made to a specific acidic value in pH to permit agitation without dilution with water and prevent the deterioration caused by deamination or others and followed by extraction with alkali, thus isolating DNA, an intermediate for drugs or food.
CONSTITUTION: After homogenized, milt of fish is adjusted to 4.5W6.5 in pH by inorganic or organic acid and stirred at 80W100°C for 0.5W2 hr. Then, an alkali aqueous solution is added to adjust the pH to over 8 and the stirring is continued, the solid part is separated and an acid is added to the filtrate to isolate deoxyribonucleic acid (DNA). The adjustment of the homogenized milt of fish to a specific pH value permits the increase in quantity to be treated per batch and the deterioration caused by deamination or others on heating can be prevented.
COPYRIGHT: (C)1980,JPO&Japio
JP7540078A 1978-06-23 1978-06-23 Isolation of deoxyribonucleic acid from milt of fish Pending JPS554308A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7540078A JPS554308A (en) 1978-06-23 1978-06-23 Isolation of deoxyribonucleic acid from milt of fish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7540078A JPS554308A (en) 1978-06-23 1978-06-23 Isolation of deoxyribonucleic acid from milt of fish

Publications (1)

Publication Number Publication Date
JPS554308A true JPS554308A (en) 1980-01-12

Family

ID=13575081

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7540078A Pending JPS554308A (en) 1978-06-23 1978-06-23 Isolation of deoxyribonucleic acid from milt of fish

Country Status (1)

Country Link
JP (1) JPS554308A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4985552A (en) * 1986-04-17 1991-01-15 Crinos Industria Farmacobiologica S.P.A. Process for obtaining chemically defined and reproducible polydeoxyribonucleotides

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4985552A (en) * 1986-04-17 1991-01-15 Crinos Industria Farmacobiologica S.P.A. Process for obtaining chemically defined and reproducible polydeoxyribonucleotides

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