JPS5578255A - Simultaneous simple and quick measurement method for low and high specific gravity lipo protein cholesterol and its measuring kit - Google Patents

Simultaneous simple and quick measurement method for low and high specific gravity lipo protein cholesterol and its measuring kit

Info

Publication number
JPS5578255A
JPS5578255A JP15232478A JP15232478A JPS5578255A JP S5578255 A JPS5578255 A JP S5578255A JP 15232478 A JP15232478 A JP 15232478A JP 15232478 A JP15232478 A JP 15232478A JP S5578255 A JPS5578255 A JP S5578255A
Authority
JP
Japan
Prior art keywords
specific gravity
protein cholesterol
low
lipo protein
coloring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP15232478A
Other languages
Japanese (ja)
Inventor
Tsutomu Irikura
Koichi Takagi
Takamichi Okada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyorin Pharmaceutical Co Ltd
Original Assignee
Kyorin Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyorin Pharmaceutical Co Ltd filed Critical Kyorin Pharmaceutical Co Ltd
Priority to JP15232478A priority Critical patent/JPS5578255A/en
Publication of JPS5578255A publication Critical patent/JPS5578255A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To enable to measure quickly and simply the low and high specific gravity substances mensioned above at the same time by measuring the absorbance through the addition of coloring enzyme solution to the supernatant liquid, after selectively depositing the low specific gravity lipo protein cholesterol through the addition of reagent to the inspected serum. CONSTITUTION:The low specific gravity lipo protein cholesterol is selectively deposited by adding deposition reagent of phosphorus tungsten acid sodium-magnesium chloride to inspected serum. This deposit is separated and dissolved into potassium chloride or sodium chloride, the coloring enzyme is added to obtain the low specific gravity lipo protein cholesterol through the measurement of absorbance. On the other hand, coloring enzyme is added to the supernatant, and the amount of the high density lipo protein cholesterol is obtained with the similar means. As the coloring enzyme, cholesterolesterase, cholesteroloxidase, and addition of 4- aminoantipyrine and phenol as coloring agent to the peroxidase, are used.
JP15232478A 1978-12-08 1978-12-08 Simultaneous simple and quick measurement method for low and high specific gravity lipo protein cholesterol and its measuring kit Pending JPS5578255A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP15232478A JPS5578255A (en) 1978-12-08 1978-12-08 Simultaneous simple and quick measurement method for low and high specific gravity lipo protein cholesterol and its measuring kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15232478A JPS5578255A (en) 1978-12-08 1978-12-08 Simultaneous simple and quick measurement method for low and high specific gravity lipo protein cholesterol and its measuring kit

Publications (1)

Publication Number Publication Date
JPS5578255A true JPS5578255A (en) 1980-06-12

Family

ID=15538030

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15232478A Pending JPS5578255A (en) 1978-12-08 1978-12-08 Simultaneous simple and quick measurement method for low and high specific gravity lipo protein cholesterol and its measuring kit

Country Status (1)

Country Link
JP (1) JPS5578255A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61195359A (en) * 1985-02-25 1986-08-29 Nitsusui Seiyaku Kk Production of clear serum
CN114774513A (en) * 2022-05-20 2022-07-22 浙江伊利康生物技术有限公司 Reagent and method for measuring small and dense low-density lipoprotein cholesterol
CN114891854A (en) * 2022-05-20 2022-08-12 浙江伊利康生物技术有限公司 Reagent and kit for measuring small and dense low-density lipoprotein cholesterol

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61195359A (en) * 1985-02-25 1986-08-29 Nitsusui Seiyaku Kk Production of clear serum
CN114774513A (en) * 2022-05-20 2022-07-22 浙江伊利康生物技术有限公司 Reagent and method for measuring small and dense low-density lipoprotein cholesterol
CN114891854A (en) * 2022-05-20 2022-08-12 浙江伊利康生物技术有限公司 Reagent and kit for measuring small and dense low-density lipoprotein cholesterol

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