JPS559036A - Purification of igm - Google Patents

Purification of igm

Info

Publication number
JPS559036A
JPS559036A JP8176978A JP8176978A JPS559036A JP S559036 A JPS559036 A JP S559036A JP 8176978 A JP8176978 A JP 8176978A JP 8176978 A JP8176978 A JP 8176978A JP S559036 A JPS559036 A JP S559036A
Authority
JP
Japan
Prior art keywords
solution
igm
fractionation
cohn
subjected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP8176978A
Other languages
Japanese (ja)
Other versions
JPS6143330B2 (en
Inventor
Yatsuhiro Kamimura
Katsuhiro Uryu
Satoru Funakoshi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tanabe Pharma Corp
GC Biopharma Corp
Original Assignee
Green Cross Corp Japan
Green Cross Corp Korea
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan, Green Cross Corp Korea filed Critical Green Cross Corp Japan
Priority to JP8176978A priority Critical patent/JPS559036A/en
Publication of JPS559036A publication Critical patent/JPS559036A/en
Publication of JPS6143330B2 publication Critical patent/JPS6143330B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE: To obtain purified IgM having high stability, in an industrial scale in high efficiency, by subjecting pasty Cohn's fraction III to a combination of acrinol precipitation, polyethylene glycol fractionation, etc.
CONSTITUTION: Pasty Cohn's fraction III obtained by the Cohn's cold alcohol fractionation, is suspended in an aqueous solution of an inorganic salt (e.g. 0.5W 2.0W/V% NaCl solution), to extract IgM from the fraction. Acrinol is added to the suspension, and the insoluble components are removed. The supernatant liquid is subjected to the salting-out, and the insoluble matters are removed. Ammonium sulfate is added to the liquid, and salted out. The precipitation is dissolved in an inorganic salt solution or in distilled water, and the solution is subjected to the polyethylene glycol fractionation. The precipitate is dissolved in water. The solution is made weak acidic (esp. pH 5.2W5.4), and subjected to the isoelectric point fractionation to obtain a supernatant liquid containing pure IgM.
COPYRIGHT: (C)1980,JPO&Japio
JP8176978A 1978-07-04 1978-07-04 Purification of igm Granted JPS559036A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP8176978A JPS559036A (en) 1978-07-04 1978-07-04 Purification of igm

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP8176978A JPS559036A (en) 1978-07-04 1978-07-04 Purification of igm

Publications (2)

Publication Number Publication Date
JPS559036A true JPS559036A (en) 1980-01-22
JPS6143330B2 JPS6143330B2 (en) 1986-09-26

Family

ID=13755661

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8176978A Granted JPS559036A (en) 1978-07-04 1978-07-04 Purification of igm

Country Status (1)

Country Link
JP (1) JPS559036A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63165497A (en) * 1986-12-26 1988-07-08 ライオン株式会社 Detergent composition for bathroom and bath furnace
JP2007332080A (en) * 2006-06-15 2007-12-27 Kaneka Corp Method for purifying IgM type antibody, adsorbent for IgM type antibody recognition antigen
JP2016506953A (en) * 2013-02-06 2016-03-07 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ Protein purification method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63165497A (en) * 1986-12-26 1988-07-08 ライオン株式会社 Detergent composition for bathroom and bath furnace
JP2007332080A (en) * 2006-06-15 2007-12-27 Kaneka Corp Method for purifying IgM type antibody, adsorbent for IgM type antibody recognition antigen
JP2016506953A (en) * 2013-02-06 2016-03-07 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ Protein purification method

Also Published As

Publication number Publication date
JPS6143330B2 (en) 1986-09-26

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