JPS5592697A - Method for quantitative analysis using immobilized enzyme - Google Patents

Method for quantitative analysis using immobilized enzyme

Info

Publication number
JPS5592697A
JPS5592697A JP16516978A JP16516978A JPS5592697A JP S5592697 A JPS5592697 A JP S5592697A JP 16516978 A JP16516978 A JP 16516978A JP 16516978 A JP16516978 A JP 16516978A JP S5592697 A JPS5592697 A JP S5592697A
Authority
JP
Japan
Prior art keywords
solution
oxidizing
hydrogen peroxide
agent
immobilized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP16516978A
Other languages
Japanese (ja)
Inventor
Yoshiharu Kondo
Junichiro Kikutake
Masakazu Sugiura
Masaru Yoshida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanyo Chemical Industries Ltd
Original Assignee
Sanyo Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanyo Chemical Industries Ltd filed Critical Sanyo Chemical Industries Ltd
Priority to JP16516978A priority Critical patent/JPS5592697A/en
Publication of JPS5592697A publication Critical patent/JPS5592697A/en
Pending legal-status Critical Current

Links

Landscapes

  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine the amount to hydrogen peroxide in a solution, in high accuracy, by oxidizing a detecting agent added to the solution with the hydrogen peroxide in the presence of an immobilized peroxidase as a catalyst, and determining the oxidized detecting agent.
CONSTITUTION: Hydrogen peroxide in a solution is determined by (1) oxidizing an intermediate electron transfer material such as metal ions having two or more ionic valences, with the hydrogen peroxide in the presence of an immobilized peroxidase as a catalyst, (2) separating the immobilized enzyme from the solution containing the oxidized intermediate electron transfer material, (3) adding a detecting agnet to the solution thereby oxidizing the agent, and (4) determining the amount of the oxidized detecting agent in the solution. A compound such as saccharides, purine derivatives, etc. can also be determined by (1) oxidizing the compound in the presence of an immobilized oxidase, and (2) carrying out the above procedures using the produced hydrogen peroxide as an oxidizing agent.
COPYRIGHT: (C)1980,JPO&Japio
JP16516978A 1978-12-29 1978-12-29 Method for quantitative analysis using immobilized enzyme Pending JPS5592697A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP16516978A JPS5592697A (en) 1978-12-29 1978-12-29 Method for quantitative analysis using immobilized enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP16516978A JPS5592697A (en) 1978-12-29 1978-12-29 Method for quantitative analysis using immobilized enzyme

Publications (1)

Publication Number Publication Date
JPS5592697A true JPS5592697A (en) 1980-07-14

Family

ID=15807163

Family Applications (1)

Application Number Title Priority Date Filing Date
JP16516978A Pending JPS5592697A (en) 1978-12-29 1978-12-29 Method for quantitative analysis using immobilized enzyme

Country Status (1)

Country Link
JP (1) JPS5592697A (en)

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