JPS5599191A - Purification of kallikrein in human urine - Google Patents

Purification of kallikrein in human urine

Info

Publication number
JPS5599191A
JPS5599191A JP595279A JP595279A JPS5599191A JP S5599191 A JPS5599191 A JP S5599191A JP 595279 A JP595279 A JP 595279A JP 595279 A JP595279 A JP 595279A JP S5599191 A JPS5599191 A JP S5599191A
Authority
JP
Japan
Prior art keywords
kallikrein
human urine
purification
sepharose
arginine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP595279A
Other languages
Japanese (ja)
Inventor
Sadaaki Iwanaga
Nobuhisa Ogawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mochida Pharmaceutical Co Ltd
Original Assignee
Mochida Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mochida Pharmaceutical Co Ltd filed Critical Mochida Pharmaceutical Co Ltd
Priority to JP595279A priority Critical patent/JPS5599191A/en
Publication of JPS5599191A publication Critical patent/JPS5599191A/en
Pending legal-status Critical Current

Links

Landscapes

  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To purify kallikrein (K) in human urine effectively, by treating a K-containing solution with arginine-Sepharose.
CONSTITUTION: Human urine or a crude solution contaning kallikrein (K) is adjusted to pH7.0W9.0, and brought into contact with arginine-Sepharose (A) adjusted to the same pH, preferably about 8.0, to have K adsorbed on A specifically. The adsorbed K is then eluted with a buffer of pH7.0W9.0 containing 0.1W0.5mol of sodium chloride.
COPYRIGHT: (C)1980,JPO&Japio
JP595279A 1979-01-18 1979-01-18 Purification of kallikrein in human urine Pending JPS5599191A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP595279A JPS5599191A (en) 1979-01-18 1979-01-18 Purification of kallikrein in human urine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP595279A JPS5599191A (en) 1979-01-18 1979-01-18 Purification of kallikrein in human urine

Publications (1)

Publication Number Publication Date
JPS5599191A true JPS5599191A (en) 1980-07-28

Family

ID=11625222

Family Applications (1)

Application Number Title Priority Date Filing Date
JP595279A Pending JPS5599191A (en) 1979-01-18 1979-01-18 Purification of kallikrein in human urine

Country Status (1)

Country Link
JP (1) JPS5599191A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4780209A (en) * 1984-06-06 1988-10-25 Japan Chemical Research Co., Ltd. Process for concentrating and separating trypsin inhibitor and kallidinogenase in human urine
JP2006127180A (en) * 2004-10-29 2006-05-18 Nec Infrontia Corp Pc card

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4780209A (en) * 1984-06-06 1988-10-25 Japan Chemical Research Co., Ltd. Process for concentrating and separating trypsin inhibitor and kallidinogenase in human urine
JP2006127180A (en) * 2004-10-29 2006-05-18 Nec Infrontia Corp Pc card

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