JPS56112902A - Novel protein polysaccharide wak - Google Patents

Novel protein polysaccharide wak

Info

Publication number
JPS56112902A
JPS56112902A JP1429380A JP1429380A JPS56112902A JP S56112902 A JPS56112902 A JP S56112902A JP 1429380 A JP1429380 A JP 1429380A JP 1429380 A JP1429380 A JP 1429380A JP S56112902 A JPS56112902 A JP S56112902A
Authority
JP
Japan
Prior art keywords
wak
protein polysaccharide
aspergillus oryzae
precipitate
antitumoral
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP1429380A
Other languages
Japanese (ja)
Other versions
JPS5724358B2 (en
Inventor
Keijiro Miyata
Jun Matsumoto
Kenichi Edanami
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wakamoto Pharmaceutical Co Ltd
Original Assignee
Wakamoto Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wakamoto Pharmaceutical Co Ltd filed Critical Wakamoto Pharmaceutical Co Ltd
Priority to JP1429380A priority Critical patent/JPS56112902A/en
Publication of JPS56112902A publication Critical patent/JPS56112902A/en
Publication of JPS5724358B2 publication Critical patent/JPS5724358B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

PURPOSE: To obtain an antitumoral protein polysaccharide WAK, by culturing protein polysaccharide WAK-producing bacteria such as Aspergillus oryzae A155, Aspergillus oryzae IFO4176 or Aspergillus oryzae NK.
CONSTITUTION: Protein polysaccharide WAK-producing bacteria are cultured in a culture medium containing embryos of crop such as rice or wheat. The culture product obtained is extracted with, for example, hot water. To the extract is added a metal salt such as CaCl2 to separate nucleic acids. To this solution is added CH3OH and the precipitate formed is dissolved in water. This solution is mixed again with CH3OH to form a precipitate. This precipitate is washed successively with 70% C2H5OH and 95% C2H5OH, and then dried to crude powder. Then, this crude powder is fractionated to obtain an eluate containing antitumoral components by a 0.1W0.4 M-NaCl concentration gradient method using, for example, DEAE cellulose and then title WAK is collected by concentration, salt removal and gel filtration of the eluate through, for example, Sephadex G-200.
COPYRIGHT: (C)1981,JPO&Japio
JP1429380A 1980-02-09 1980-02-09 Novel protein polysaccharide wak Granted JPS56112902A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1429380A JPS56112902A (en) 1980-02-09 1980-02-09 Novel protein polysaccharide wak

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1429380A JPS56112902A (en) 1980-02-09 1980-02-09 Novel protein polysaccharide wak

Publications (2)

Publication Number Publication Date
JPS56112902A true JPS56112902A (en) 1981-09-05
JPS5724358B2 JPS5724358B2 (en) 1982-05-24

Family

ID=11857037

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1429380A Granted JPS56112902A (en) 1980-02-09 1980-02-09 Novel protein polysaccharide wak

Country Status (1)

Country Link
JP (1) JPS56112902A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107746433B (en) * 2017-09-14 2021-03-16 天津科技大学 A kind of preparation method of cellulose-based antibacterial material dialdehyde cellulose-lysine

Also Published As

Publication number Publication date
JPS5724358B2 (en) 1982-05-24

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