JPS56148295A - Preparation of l-glutamic acid by fermentation method - Google Patents

Preparation of l-glutamic acid by fermentation method

Info

Publication number
JPS56148295A
JPS56148295A JP5100180A JP5100180A JPS56148295A JP S56148295 A JPS56148295 A JP S56148295A JP 5100180 A JP5100180 A JP 5100180A JP 5100180 A JP5100180 A JP 5100180A JP S56148295 A JPS56148295 A JP S56148295A
Authority
JP
Japan
Prior art keywords
bacteria
glutamic acid
dna
substance
ability
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP5100180A
Other languages
Japanese (ja)
Inventor
Takayasu Tsuchida
Kiyoshi Miwa
Shigeru Nakamori
Haruo Momose
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Priority to JP5100180A priority Critical patent/JPS56148295A/en
Priority to GB811959A priority patent/GB2076853B/en
Priority to DE19813115515 priority patent/DE3115515A1/en
Priority to IT21232/81A priority patent/IT1136577B/en
Priority to FR8107788A priority patent/FR2482133A1/en
Priority to US06/255,169 priority patent/US4427773A/en
Publication of JPS56148295A publication Critical patent/JPS56148295A/en
Priority to US06/434,853 priority patent/US4778762A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/13—Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE:To improve the productivity of the titled substance, by extracting DNA having the genetic information related to the biosynthesis of the titled substance from bacteria, having the ability to produce the titled substance, and belonging to the genus Brevibacterium, incorporating the DNA into a vector, and introducing the recombined gene into the bacteria. CONSTITUTION:Chromosomic deoxyribonucleic acid (DNA) carrying the genetic information related to the biosynthesis of L-glutamic acid is extracted from bacteria, e.g. Brevibacterium lactofermentum ATCC 13869, belonging to the genus Brevibacterium, and having the ability of produce the L-glutamic acid and then treated with a restriction endonuclease. A plasmid or phage obtained from bacteria capable of donating the DNA or a variant strain deficient in the ability to produce the L- glutamic acid derived from the donor bacteria is then treated with the endonuclease and mixed with the chromosomic DNA to give a combined substance. The resultant combined substance is then introduced into the bacteria or a variant strain having the L-glutamic acid requirement thereof by the transforming method to give the aimed transformed strain.
JP5100180A 1980-04-17 1980-04-17 Preparation of l-glutamic acid by fermentation method Pending JPS56148295A (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP5100180A JPS56148295A (en) 1980-04-17 1980-04-17 Preparation of l-glutamic acid by fermentation method
GB811959A GB2076853B (en) 1980-04-17 1981-01-15 L-glutamic acid producing microorganisms
DE19813115515 DE3115515A1 (en) 1980-04-17 1981-04-16 METHOD FOR THE FERMENTATIVE PRODUCTION OF L-GLUTAMIC ACID
IT21232/81A IT1136577B (en) 1980-04-17 1981-04-16 PROCEDURE FOR PRODUCING L-GLUTAMIC ACID BY FERMENTATION
FR8107788A FR2482133A1 (en) 1980-04-17 1981-04-17 PROCESS FOR THE PRODUCTION OF L-GLUTAMIC ACID BY FERMENTATION USING MICROORGANISMS OBTAINED BY GENETIC TRANSFORMATION
US06/255,169 US4427773A (en) 1980-04-17 1981-04-17 Method for producing L-glutamic acid by fermentation
US06/434,853 US4778762A (en) 1980-04-17 1982-10-18 Plasmid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5100180A JPS56148295A (en) 1980-04-17 1980-04-17 Preparation of l-glutamic acid by fermentation method

Publications (1)

Publication Number Publication Date
JPS56148295A true JPS56148295A (en) 1981-11-17

Family

ID=12874535

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5100180A Pending JPS56148295A (en) 1980-04-17 1980-04-17 Preparation of l-glutamic acid by fermentation method

Country Status (1)

Country Link
JP (1) JPS56148295A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57186492A (en) * 1981-04-17 1982-11-16 Kyowa Hakko Kogyo Co Ltd Transformation of bacterium
JPS58105999A (en) * 1981-12-17 1983-06-24 Kyowa Hakko Kogyo Co Ltd Novel vector-plasmid
JPS58126789A (en) * 1981-12-29 1983-07-28 Kyowa Hakko Kogyo Co Ltd Method for developing genetic character
JPS58192900A (en) * 1982-05-04 1983-11-10 Ajinomoto Co Inc Complex plasmid
JPS58216199A (en) * 1982-06-10 1983-12-15 Ajinomoto Co Inc Hybrid plasmid

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5441392A (en) * 1977-09-07 1979-04-02 Mitsubishi Chem Ind Ltd Preparation of plasmid

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5441392A (en) * 1977-09-07 1979-04-02 Mitsubishi Chem Ind Ltd Preparation of plasmid

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57186492A (en) * 1981-04-17 1982-11-16 Kyowa Hakko Kogyo Co Ltd Transformation of bacterium
JPS58105999A (en) * 1981-12-17 1983-06-24 Kyowa Hakko Kogyo Co Ltd Novel vector-plasmid
JPS58126789A (en) * 1981-12-29 1983-07-28 Kyowa Hakko Kogyo Co Ltd Method for developing genetic character
JPS58192900A (en) * 1982-05-04 1983-11-10 Ajinomoto Co Inc Complex plasmid
JPS58216199A (en) * 1982-06-10 1983-12-15 Ajinomoto Co Inc Hybrid plasmid

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