JPS5615687A - Immobilized protease and its preparation - Google Patents
Immobilized protease and its preparationInfo
- Publication number
- JPS5615687A JPS5615687A JP8970979A JP8970979A JPS5615687A JP S5615687 A JPS5615687 A JP S5615687A JP 8970979 A JP8970979 A JP 8970979A JP 8970979 A JP8970979 A JP 8970979A JP S5615687 A JPS5615687 A JP S5615687A
- Authority
- JP
- Japan
- Prior art keywords
- protease
- fibroin
- aqueous solution
- solution
- alkali
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 6
- 239000004365 Protease Substances 0.000 title abstract 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 6
- 108010022355 Fibroins Proteins 0.000 abstract 4
- 239000007864 aqueous solution Substances 0.000 abstract 4
- 108090000145 Bacillolysin Proteins 0.000 abstract 2
- 102000035092 Neutral proteases Human genes 0.000 abstract 2
- 108091005507 Neutral proteases Proteins 0.000 abstract 2
- 239000003513 alkali Substances 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 108010013296 Sericins Proteins 0.000 abstract 1
- 229910052783 alkali metal Inorganic materials 0.000 abstract 1
- -1 alkali metal salt Chemical class 0.000 abstract 1
- 238000011033 desalting Methods 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To produce an immobilized protease having improved thermal stability and pH stability industrially and at a low cost, by incorporating an alkali protease and/or neutral protease in a specific wt% into fibroin.
CONSTITUTION: An aqueous solution of fibroin is incorporated with an aqueous solution of an alkali protease and/or neutral protease adjusted to pH5W5.5. The fibroin and the protease are salted out and precipitated by the use of an inorganic and/or organic salt, and the resulted precipitate is washed with water and dried to prepare an immobilized protease. The aqueous solution of fibroin is obtaned by dissolving sericin scoured and removed from silk, e.g. raw silk or cocoon, in an aqueous solution of an alkali metal salt, or further dialyzing and desalting the solution, and the concentration of the equeous solution is preferably 2W20wt%.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8970979A JPS6013672B2 (en) | 1979-07-13 | 1979-07-13 | Immobilized protease and its production method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8970979A JPS6013672B2 (en) | 1979-07-13 | 1979-07-13 | Immobilized protease and its production method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5615687A true JPS5615687A (en) | 1981-02-14 |
| JPS6013672B2 JPS6013672B2 (en) | 1985-04-09 |
Family
ID=13978291
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8970979A Expired JPS6013672B2 (en) | 1979-07-13 | 1979-07-13 | Immobilized protease and its production method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6013672B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62188177U (en) * | 1986-05-22 | 1987-11-30 | ||
| JP3024212U (en) * | 1995-10-30 | 1996-05-17 | 有限会社ゴーイング東京 | Filter element for engine air cleaner |
-
1979
- 1979-07-13 JP JP8970979A patent/JPS6013672B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6013672B2 (en) | 1985-04-09 |
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