JPS5639800A - Method for specific quantitative analysis of pyruvic acid - Google Patents

Method for specific quantitative analysis of pyruvic acid

Info

Publication number
JPS5639800A
JPS5639800A JP11490379A JP11490379A JPS5639800A JP S5639800 A JPS5639800 A JP S5639800A JP 11490379 A JP11490379 A JP 11490379A JP 11490379 A JP11490379 A JP 11490379A JP S5639800 A JPS5639800 A JP S5639800A
Authority
JP
Japan
Prior art keywords
acid
pyruvic acid
pyruvic
amino acid
hypotaurine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP11490379A
Other languages
Japanese (ja)
Other versions
JPS6319160B2 (en
Inventor
Kenji Soda
Toshiko Hirasawa
Kiyoyoshi Touyama
Kazuo Yonaha
Hidehiko Tanaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KOKUSAI SHIYAKU KK
Original Assignee
KOKUSAI SHIYAKU KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KOKUSAI SHIYAKU KK filed Critical KOKUSAI SHIYAKU KK
Priority to JP11490379A priority Critical patent/JPS5639800A/en
Publication of JPS5639800A publication Critical patent/JPS5639800A/en
Publication of JPS6319160B2 publication Critical patent/JPS6319160B2/ja
Granted legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine pyruvic acid accurately even in the presence of other salts, by subjecting a pyruvic acid solutio containing a hypotaurine to the action of an ω-amino acid-pyruvic acid transaminase to generate SO2 gas.
CONSTITUTION: A pyruvic acid solution containing a hypotaurine is subjected to the action of an ω-amino acid -pyruvic acid tansaminase which is an enzyme having very high specificity for the pruvic acid. The amount of SO2 gas in th resultant substances is measured to determine the pyruvic acid specifically. The purified and crystallized ω- amino acid -pyruvic acid transaminase has a molecular weight of 167,000±3,000, shows the maximum activity at pH8.5W10.5, and is specifically active only for the pyruvic acid. Thus, the determined value is not affected even by the presence of an amino acid, e.g. glutamic acid, methionine or alanine, α-ketoglutaric acid, α-ketobutyric acid, etc.
COPYRIGHT: (C)1981,JPO&Japio
JP11490379A 1979-09-07 1979-09-07 Method for specific quantitative analysis of pyruvic acid Granted JPS5639800A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11490379A JPS5639800A (en) 1979-09-07 1979-09-07 Method for specific quantitative analysis of pyruvic acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11490379A JPS5639800A (en) 1979-09-07 1979-09-07 Method for specific quantitative analysis of pyruvic acid

Publications (2)

Publication Number Publication Date
JPS5639800A true JPS5639800A (en) 1981-04-15
JPS6319160B2 JPS6319160B2 (en) 1988-04-21

Family

ID=14649507

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11490379A Granted JPS5639800A (en) 1979-09-07 1979-09-07 Method for specific quantitative analysis of pyruvic acid

Country Status (1)

Country Link
JP (1) JPS5639800A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01139893A (en) * 1987-11-26 1989-06-01 Hakuto Kagaku Kk Anti-clogging agent and method for preventing clogging of suction rolls for paper machines

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01139893A (en) * 1987-11-26 1989-06-01 Hakuto Kagaku Kk Anti-clogging agent and method for preventing clogging of suction rolls for paper machines

Also Published As

Publication number Publication date
JPS6319160B2 (en) 1988-04-21

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