JPS5647763A - Determination of proteinic matter having fat-mobilizing activity in canceous-cell secretion such as abdominal dropsy or fluid in thoracic cavity - Google Patents
Determination of proteinic matter having fat-mobilizing activity in canceous-cell secretion such as abdominal dropsy or fluid in thoracic cavityInfo
- Publication number
- JPS5647763A JPS5647763A JP12462779A JP12462779A JPS5647763A JP S5647763 A JPS5647763 A JP S5647763A JP 12462779 A JP12462779 A JP 12462779A JP 12462779 A JP12462779 A JP 12462779A JP S5647763 A JPS5647763 A JP S5647763A
- Authority
- JP
- Japan
- Prior art keywords
- fat
- toxohormone
- fractions
- canceous
- matter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 206010030113 Oedema Diseases 0.000 title abstract 2
- 230000003187 abdominal effect Effects 0.000 title abstract 2
- 230000000694 effects Effects 0.000 title abstract 2
- 239000012530 fluid Substances 0.000 title abstract 2
- 230000028327 secretion Effects 0.000 title abstract 2
- 210000000115 thoracic cavity Anatomy 0.000 title abstract 2
- 206010028980 Neoplasm Diseases 0.000 abstract 2
- 239000007853 buffer solution Substances 0.000 abstract 2
- 201000011510 cancer Diseases 0.000 abstract 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 102000009027 Albumins Human genes 0.000 abstract 1
- 108010088751 Albumins Proteins 0.000 abstract 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 235000014113 dietary fatty acids Nutrition 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 abstract 1
- 239000000194 fatty acid Substances 0.000 abstract 1
- 229930195729 fatty acid Natural products 0.000 abstract 1
- 150000004665 fatty acids Chemical class 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 230000001483 mobilizing effect Effects 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
PURPOSE: To accurately determine proteinic matter (toxohormone L) having fat- mobilizing activity by an experiment not on a living body but in a test tube.
CONSTITUTION: From such canceous-cell secretion as abdominal dropsy or fluid in the thoracic cavity of a cancer patient or cancer-carrying animal, the fractions precipitated by an ammonium sulfate fractionation of 40W70% saturation are collected and treated on the ion exchanger chromatography by the gradient elution technique. As the ion exchanger, DEAE cellulose is specially preferable. Then the fractions having fat-mobilizing activity are lyophilized and filtered by using a hydrophilic gel. From the fractions thus obtained, toxohormone L can be separated and refined within a certain pH range by the isoelectric point cataphoresis. A proper number of fat-tissue slices are added to a Krebs-Ringer carbonic acid buffer solution, and to this a toxohormone L aqueous solution and a Krebs-Ringer carbonic acid buffer solution containing 5% albumin are added to determine the liberated fatty acid by the Dole's method.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP54124627A JPS592862B2 (en) | 1979-09-26 | 1979-09-26 | Method for quantifying protein substances with fat mobilization activity in cancer cell secretions such as ascites and pleural effusions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP54124627A JPS592862B2 (en) | 1979-09-26 | 1979-09-26 | Method for quantifying protein substances with fat mobilization activity in cancer cell secretions such as ascites and pleural effusions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5647763A true JPS5647763A (en) | 1981-04-30 |
| JPS592862B2 JPS592862B2 (en) | 1984-01-20 |
Family
ID=14890088
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP54124627A Expired JPS592862B2 (en) | 1979-09-26 | 1979-09-26 | Method for quantifying protein substances with fat mobilization activity in cancer cell secretions such as ascites and pleural effusions |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS592862B2 (en) |
-
1979
- 1979-09-26 JP JP54124627A patent/JPS592862B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS592862B2 (en) | 1984-01-20 |
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