JPS5653465A - Improved method in measurement of enzyme immunity - Google Patents
Improved method in measurement of enzyme immunityInfo
- Publication number
- JPS5653465A JPS5653465A JP12925279A JP12925279A JPS5653465A JP S5653465 A JPS5653465 A JP S5653465A JP 12925279 A JP12925279 A JP 12925279A JP 12925279 A JP12925279 A JP 12925279A JP S5653465 A JPS5653465 A JP S5653465A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- immune
- measurement
- constituent
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005259 measurement Methods 0.000 title abstract 5
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 230000036039 immunity Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 239000000470 constituent Substances 0.000 abstract 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract 2
- 239000003638 chemical reducing agent Substances 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 230000008105 immune reaction Effects 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 abstract 2
- 108010044467 Isoenzymes Proteins 0.000 abstract 1
- 230000000274 adsorptive effect Effects 0.000 abstract 1
- 239000000427 antigen Substances 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 235000010323 ascorbic acid Nutrition 0.000 abstract 1
- 229960005070 ascorbic acid Drugs 0.000 abstract 1
- 239000011668 ascorbic acid Substances 0.000 abstract 1
- 229940079877 pyrogallol Drugs 0.000 abstract 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 abstract 1
- 229940001474 sodium thiosulfate Drugs 0.000 abstract 1
- 235000019345 sodium thiosulphate Nutrition 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To enable accurate measurement of an immune constituent in a sample by a method wherein a reductant is added to an immune reaction system and thereby the effect of various proteins and the like contained in the sample is removed when the measurement of the enzyme immunity is performed by the use of an enzyme-marked immune constituent.
CONSTITUTION: When the immune constituent of a microcomponent in an organism, such as, for example, antigen, antibody and further a receptor and isozyme, is measured by a method of enzyme immunity measurement wherein the enzyme- marked immune constituent is used, the reductant such as sodium thiosulfate, ascorbic acid and pyrogallol is added to the immune reaction system in such a degree that the final density thereof is about 0.02W0.3%. By this constitution, a phenomenon that various proteins contained in the sample make the adsorptive reaction nondifferentially with the enzyme of the enzyme-marked immune constituent and thereby give a considerable effect to the value of measurement is prevented.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12925279A JPS5653465A (en) | 1979-10-05 | 1979-10-05 | Improved method in measurement of enzyme immunity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12925279A JPS5653465A (en) | 1979-10-05 | 1979-10-05 | Improved method in measurement of enzyme immunity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5653465A true JPS5653465A (en) | 1981-05-13 |
| JPS6217707B2 JPS6217707B2 (en) | 1987-04-18 |
Family
ID=15004963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12925279A Granted JPS5653465A (en) | 1979-10-05 | 1979-10-05 | Improved method in measurement of enzyme immunity |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5653465A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59104555A (en) * | 1982-11-08 | 1984-06-16 | アボツト・ラボラトリ−ズ | Method of measuring terminal deoxynucleotidyltransferase |
| JPH06189760A (en) * | 1992-09-14 | 1994-07-12 | E I Du Pont De Nemours & Co | Composition for stabilizing enzymatic activity and immunoreaction of creatine kinase and creatine kinase isoenzyme |
| JPH10509805A (en) * | 1995-08-03 | 1998-09-22 | デイド、ケミストリー、システムズ、インコーポレイテッド | Post-synthesis chemical modification of particle reagents |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0461794U (en) * | 1990-10-01 | 1992-05-27 | ||
| JP6520940B2 (en) * | 2014-06-20 | 2019-05-29 | コニカミノルタ株式会社 | Sandwich type assay using labeled lectin and kit therefor |
-
1979
- 1979-10-05 JP JP12925279A patent/JPS5653465A/en active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59104555A (en) * | 1982-11-08 | 1984-06-16 | アボツト・ラボラトリ−ズ | Method of measuring terminal deoxynucleotidyltransferase |
| JPH06189760A (en) * | 1992-09-14 | 1994-07-12 | E I Du Pont De Nemours & Co | Composition for stabilizing enzymatic activity and immunoreaction of creatine kinase and creatine kinase isoenzyme |
| JPH10509805A (en) * | 1995-08-03 | 1998-09-22 | デイド、ケミストリー、システムズ、インコーポレイテッド | Post-synthesis chemical modification of particle reagents |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6217707B2 (en) | 1987-04-18 |
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