JPS5660342A - Determination method of nitrous acid by microbe electrode - Google Patents

Determination method of nitrous acid by microbe electrode

Info

Publication number
JPS5660342A
JPS5660342A JP13675979A JP13675979A JPS5660342A JP S5660342 A JPS5660342 A JP S5660342A JP 13675979 A JP13675979 A JP 13675979A JP 13675979 A JP13675979 A JP 13675979A JP S5660342 A JPS5660342 A JP S5660342A
Authority
JP
Japan
Prior art keywords
nitrous acid
microbe
electrode
specimen
decrease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP13675979A
Other languages
Japanese (ja)
Inventor
Motohiko Hikima
Takeo Yasuda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Priority to JP13675979A priority Critical patent/JPS5660342A/en
Publication of JPS5660342A publication Critical patent/JPS5660342A/en
Pending legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To selectively, easily and rapidly measure the concentration of nitrous acid in the specimen by immersing a microbe electrode using nitrification bacteria into the specimen and detecting the decrease in the dissolved oxygen in the microbe film corresponding to the nitrous acid concentration as the decrease in output current.
CONSTITUTION: A microbe electrode 11 formed by fixing the active bacterium layer of nitrous acid oxidation becteria 1 by a thin film 3 having microholes is immersed into the specimen contg. nitrous acid. If the components such as nitrous acid and oxygen in the specimen diffuse into the microbe film 1 through the porous film 3 of the electrode 11, the microbes oxidize only the nitrous acid and consume oxygen. In proportion to this, the dissolved oxygen of the part near an oxygen electrode diaphragm 4 decreases and the output current of the microbe electrode 11 decreases. A proportional relation holds between the decrease in this output current and the concentration of nitrous acid, and therefore if the amount or rate of decrease of the output current is measured, the ammonia concentration in the specimen is obtained.
COPYRIGHT: (C)1981,JPO&Japio
JP13675979A 1979-10-23 1979-10-23 Determination method of nitrous acid by microbe electrode Pending JPS5660342A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP13675979A JPS5660342A (en) 1979-10-23 1979-10-23 Determination method of nitrous acid by microbe electrode

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13675979A JPS5660342A (en) 1979-10-23 1979-10-23 Determination method of nitrous acid by microbe electrode

Publications (1)

Publication Number Publication Date
JPS5660342A true JPS5660342A (en) 1981-05-25

Family

ID=15182832

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13675979A Pending JPS5660342A (en) 1979-10-23 1979-10-23 Determination method of nitrous acid by microbe electrode

Country Status (1)

Country Link
JP (1) JPS5660342A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04136750A (en) * 1990-09-28 1992-05-11 Haimo Kk Regulating method for microorganism film for biosensor
US5206170A (en) * 1991-03-28 1993-04-27 Nakano Vinegar Co., Ltd. Sulfur dioxide detector device embodied by using s02-oxidant bioagent
CN105758913A (en) * 2016-05-16 2016-07-13 扬州大学 Preparation method of straw cellulose-molybdenum disulfide complex modified electrode and application of straw cellulose-molybdenum disulfide complex modified electrode in detecting nitrite in water body

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS53120592A (en) * 1977-03-30 1978-10-21 Shiyuuichi Suzuki Measurement of saccharides in microorganism cell
JPS54128393A (en) * 1978-03-28 1979-10-04 Ajinomoto Kk Method of measuring volatile substance

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS53120592A (en) * 1977-03-30 1978-10-21 Shiyuuichi Suzuki Measurement of saccharides in microorganism cell
JPS54128393A (en) * 1978-03-28 1979-10-04 Ajinomoto Kk Method of measuring volatile substance

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04136750A (en) * 1990-09-28 1992-05-11 Haimo Kk Regulating method for microorganism film for biosensor
US5206170A (en) * 1991-03-28 1993-04-27 Nakano Vinegar Co., Ltd. Sulfur dioxide detector device embodied by using s02-oxidant bioagent
CN105758913A (en) * 2016-05-16 2016-07-13 扬州大学 Preparation method of straw cellulose-molybdenum disulfide complex modified electrode and application of straw cellulose-molybdenum disulfide complex modified electrode in detecting nitrite in water body

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